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Journal : BIOTROPIA

Fiber Disruption of Betung Bamboo (Dendrocalamus asper) by Combined Fungal and Microwave Pretreatment Fatriasari, Widya; Syafii, Wasrin; Wistara, Nyoman; Syamsu, Khaswar; Prasetya, Bambang; Anita, S Heris; Risanto, Lucky
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol 22, No 2 (2015)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2015.22.2.363

Abstract

Combined fungal and microwave pretreatment is an attractive method to alter carbohydrate and lignin structure of lignocellulosic materials for improving hydrolysis process in order to convert these lignocellulosic materials to bioethanol. This study was conducted to obtain the information on the characteristic changes of cellulose and lignin after combined biologicaland microwave pretreatment of Betung Bamboo. Based on our previous research, incubation for 30 days both for 5 and 10% liquid-inoculum loading of white rot fungi, Trametes versicolor, was chosen as the fungal pretreatment prior to microwave pretreatment at 30% power level for 5, 10 and 12.5 minutes. The evaluation of characteristic changes after combined pretreatment was performed using the analysis of FT-IR spectroscopy, X-Ray diffraction and SEM.FTIR spectra demonstrated that the combined pretreatment only affected the decrease of intensity bands of FTIR spectra with increasing microwave irradiation time, without any changes in the functional groups.This band intensity decrease occured on unconjugated bonds of carbohydrate peaked at 1736 (C=0 in xylan), 1373 (C-H deformation in cellulose and hemicellulose), 1165 (C-O-C vibration in cellulose and hemicellulose), and 895 (C-H deformation or C-O-C stretching at β-glicosidic linkage characteristic in cellulose) cm-1. The pretreatment decreased the hydrogen bond stretching of cellulose and the linkage between lignin and carbohydrate. This decrease of hydrogen bond was associated with crystallinity of bamboo cellulose,ilustrated by occurring structural changes.  Syringil propane units were more soluble than guiacyl moieties.  The crystallinity of cellulose tended to increase slightly due to the cleavage of the amorphous fraction. SEM images illustrated that the pretreatment disrupted the fiber structure while the longer microwave irradiation, the greater fiber degradation level. Prolonging microwave irradiation on 5% inoculum loading decreased lignin degrading ability. Crystalline allomorph of 5% inoculum loading for 10 minutes showed that Iβ (triclinic) structure of cellulose has transformed into monoclinic (Iα) structure.Key words: betung bamboo, combined biological andmicrowave pretreatment, cellulose and lignin changes, FTIR, SEM, XRD
Co-Authors . Gunawan . Suharyanto A. Aziz Darwis A. Herryandie Abdul Aziz Darwis ABDULLAH, M Irfani ABDULLAH, M Irfani Aida Farida Anas M. Fauzi Anas M. Fauzi Anas Miftah Fauzi Ani Suryani Anwar Nur Arkenan, Yandra Asep Riswoko ASTUTIATI NURHASANAH Bambang Prasetya Dede Rais Diana Dewi, Diana DIANA NURANI Djoko Said Damardjati Djumali Mangunwidjaja Dwi Purnomo Dwi Setyaningsih DYAH NOOR HIDAYATI E. Gumbira Said E. Gumbira-Sa’id E. Mardliyati Endang Gumbira Said Endang Warsiki Eni Dwi Islamiati, Eni Dwi ERWAHYUNI ENDANG PRABANDARI Erwahyuni Prabandari, Erwahyuni Etik Mardliyati, Etik Etty Riani Fitriani Kasim Gamma Irca Pradikta Gumbira-Sa'id, E Gumbira-Sa’id, E. Han Roliadi Hendri Muchtar, Hendri Iftachul Farida Illah Sailah Irawadi Jamaran IS HELIANTI Lanjar Sumarno Liesbetini Haditjaroko Liesbetini Hartoto Lucky Risanto M. ANWAR NUR Misgiyarta, nFN Muhammad Daud Muhammad Tasrif Mulyorini Rahayuningsih Munarso, S Joni N. A. Evalia Nabilah, Siti Nabilah, Siti Nastiti Siswi Indrasti NIKNIK NURHAYATI Nur Atifah Nur Richana Nyoman J Wistara Nyoman Jaya Wistara, Nyoman Nyoman WISTARA PERMATASARI, Shinta Prabandani, Erwahyuni E PUJI LESTARI Purbowati, Ike Sitoresmi M PUTRI, FENTRI PARAMITHA Rini Purnawati Rofiq Sunaryanto S Heris Anita, S Heris Sarifah Nurjanah Sri, Herastuti Sudirman Sudirman Suprihatin Suprihatin Suryadarma, Prayoga TRI-PANJI, . TRI-PANJI, . Tun T. Irawadi, Tun T. Tun Tedja Irawadi UNTUNG MURDIYATMO Wahono Sumaryono Wasrin Safii Wasrin Syafii Widya FATRIASARI Yandra Arkeman Yeni, Gustri Yernisa Yernisa