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Journal : Jurnal Mikrobiologi Indonesia

Analysis of Reducing Sugars on Hydrolysis of Cassava Starch by a Thermostable a-Amylasefrom Bacillus stearothemophilus TII 12 Lestari, Puji; Darwis, Abdul Aziz; Syamsu, Khaswar; Richana, Nur; Damardjati, Djoko Said
Jurnal Mikrobiologi Indonesia Vol 6, No 1 (2001): JURNAL MIKROBIOLOGI INDONESIA
Publisher : Jurnal Mikrobiologi Indonesia

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Abstract

The product of a-amylase hydrolysis was evaluated for their application In sugar syrup Industry. The objectives ofthis experiment were to determine the enzyme production of Bacillus stearothermophilus TH,2 in a five liter bioreactorand to analyze its hydrolysis product on cassava starch using thin layer chromatography and high performance liquidchromatography. The bacterium was cultured in the bloreactor for 48 hours, and then the biomass, enzyme activity,protein and reducing sugar contents in the filtrate were evaluated In the course of cultivation. The strain secreted anextraceiiuiar a-amylase in the optimal condition at pH 6.5, 5O´C, agitation of 300 rpm and aeration of 1.5 vvm for 24hours. The highest activity of a.amylase and reducing sugar content of 1 068.87 U/mI and 4.48 g/l respectively wereobtained after 24 hours incubation. Hydrolysis products by the crude enzyme on cassava starch were evaluated atdifferent Incubation time. In the course of incubation the content of glucose, dextrin, maltose and oligosaccharideswere increasing. After 24 hours the concentration of glucose and maltose reached 51 970 and 10 090 ppm respectively.Based on the enzymatic products, we concluded that thermostabie a.amylsse produced by B. slearothermophilus was aaendo-a-amylase.
Co-Authors . Gunawan . Suharyanto A. Aziz Darwis A. Herryandie Abdul Aziz Darwis ABDULLAH, M Irfani ABDULLAH, M Irfani Aida Farida Anas M. Fauzi Anas M. Fauzi Anas Miftah Fauzi Ani Suryani Anwar Nur Arkenan, Yandra Asep Riswoko ASTUTIATI NURHASANAH Bambang Prasetya Dede Rais Diana Dewi, Diana DIANA NURANI Djoko Said Damardjati Djumali Mangunwidjaja Dwi Purnomo Dwi Setyaningsih DYAH NOOR HIDAYATI E. Gumbira Said E. Gumbira-Sa’id E. Mardliyati Endang Gumbira Said Endang Warsiki Eni Dwi Islamiati, Eni Dwi ERWAHYUNI ENDANG PRABANDARI Erwahyuni Prabandari, Erwahyuni Etik Mardliyati, Etik Etty Riani Fitriani Kasim Gamma Irca Pradikta Gumbira-Sa'id, E Gumbira-Sa’id, E. Han Roliadi Hendri Muchtar, Hendri Iftachul Farida Illah Sailah Irawadi Jamaran IS HELIANTI Lanjar Sumarno Liesbetini Haditjaroko Liesbetini Hartoto Lucky Risanto M. ANWAR NUR Misgiyarta, nFN Muhammad Daud Muhammad Tasrif Mulyorini Rahayuningsih Munarso, S Joni N. A. Evalia Nabilah, Siti Nabilah, Siti Nastiti Siswi Indrasti NIKNIK NURHAYATI Nur Atifah Nur Richana Nyoman J Wistara Nyoman Jaya Wistara, Nyoman Nyoman WISTARA PERMATASARI, Shinta Prabandani, Erwahyuni E PUJI LESTARI Purbowati, Ike Sitoresmi M PUTRI, FENTRI PARAMITHA Rini Purnawati Rofiq Sunaryanto S Heris Anita, S Heris Sarifah Nurjanah Sri, Herastuti Sudirman Sudirman Suprihatin Suprihatin Suryadarma, Prayoga TRI-PANJI, . TRI-PANJI, . Tun T. Irawadi, Tun T. Tun Tedja Irawadi UNTUNG MURDIYATMO Wahono Sumaryono Wasrin Safii Wasrin Syafii Widya FATRIASARI Yandra Arkeman Yeni, Gustri Yernisa Yernisa