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Journal : TEKNIK

Optimization of Penicillium Lagena Medium Cultivication on Antifungal Pathogen of Phellinus Lamaoensis Using Surface Methode Nabilah, Siti; Sunaryanto, Rofiq; Syamsu, Khaswar
TEKNIK Vol 38, No 2 (2017): (Desember 2017)
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/teknik.v38i2.10306

Abstract

Phellinus lamaoensis (Murr.) Hein is fungal pathogen that can cause brown root rot disease in cocoa, tea, rubber, and coffee plants. Endophytic fungi, Penicillium lagena, isolated from bandotan (Ageratum conyzoides Linn.), medicinal plant, is able to inhibit the growth of pathogenic, P. lamaoensis. The effect of carbon source, nitrogen source, and mineral solution was studied. Lactose, yeast extract, and mineral solution were media components which showed significant effect toward production of P. lagena active compound. Composition optimization of these three medium components was done by response surface methodology (RSM). The Optimal response region of the significant factor was predicted by using a second order polynomial model with statistical design, central composite design (CCD). Higest production of P. lagena active compound by quadratic model was predicted to be 69.233%  with medium composition 44.77 g L-1 lactose, 13.02 g L-1 yeast extract, and 15.95 mL L-1 mineral solution. Verification value in laboratory is 58.365%, lower 15.7% than its prediction. Optimization increase P. lagena active compound 9 fold compared to unoptimize media.
Co-Authors . Gunawan . Suharyanto A. Aziz Darwis A. Herryandie Abdul Aziz Darwis ABDULLAH, M Irfani ABDULLAH, M Irfani Aida Farida Anas M. Fauzi Anas M. Fauzi Anas Miftah Fauzi Ani Suryani Anwar Nur Arkenan, Yandra Asep Riswoko ASTUTIATI NURHASANAH Bambang Prasetya Dede Rais Diana Dewi, Diana DIANA NURANI Djoko Said Damardjati Djumali Mangunwidjaja Dwi Purnomo Dwi Setyaningsih DYAH NOOR HIDAYATI E. Gumbira Said E. Gumbira-Sa’id E. Mardliyati Endang Gumbira Said Endang Warsiki Eni Dwi Islamiati, Eni Dwi ERWAHYUNI ENDANG PRABANDARI Erwahyuni Prabandari, Erwahyuni Etik Mardliyati, Etik Etty Riani Fitriani Kasim Gamma Irca Pradikta Gumbira-Sa'id, E Gumbira-Sa’id, E. Han Roliadi Hendri Muchtar, Hendri Iftachul Farida Illah Sailah Irawadi Jamaran IS HELIANTI Lanjar Sumarno Liesbetini Haditjaroko Liesbetini Hartoto Lucky Risanto M. ANWAR NUR Misgiyarta, nFN Muhammad Daud Muhammad Tasrif Mulyorini Rahayuningsih Munarso, S Joni N. A. Evalia Nabilah, Siti Nabilah, Siti Nastiti Siswi Indrasti NIKNIK NURHAYATI Nur Atifah Nur Richana Nyoman J Wistara Nyoman Jaya Wistara, Nyoman Nyoman WISTARA PERMATASARI, Shinta Prabandani, Erwahyuni E PUJI LESTARI Purbowati, Ike Sitoresmi M PUTRI, FENTRI PARAMITHA Rini Purnawati Rofiq Sunaryanto S Heris Anita, S Heris Sarifah Nurjanah Sri, Herastuti Sudirman Sudirman Suprihatin Suprihatin Suryadarma, Prayoga TRI-PANJI, . TRI-PANJI, . Tun T. Irawadi, Tun T. Tun Tedja Irawadi UNTUNG MURDIYATMO Wahono Sumaryono Wasrin Safii Wasrin Syafii Widya FATRIASARI Yandra Arkeman Yeni, Gustri Yernisa Yernisa