M. Syaifudin
Program Studi Budidaya Perairan, Fakultas Pertanian, Universitas Sriwijaya

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Relation between broodstock number and spawning frequency and egg production of humpback grouper (Cromileptes altivelis)

Jurnal Akuakultur Indonesia Vol 6, No 2 (2007): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

This study was performed to determine spawning frequency, number of ovulated egg and spawning time of humpback grouper (Cromileptes altivelis) broodstock in hatchery.  Broodstock of 20-83 fish in weight of 1.5-2.0 kg were reared in circular concrete tank 225 m3.  The results showed that increasing in number of broodstock increases spawning frequency (R2= 0.694), and ovulated eggs number was also increased (R2= 0.828).  Spawning of humpback grouper can occur in the third to fourth week in every month. Keywords: reproductive biology, spawning, Cromileptes altivelis   ABSTRAK Penelitian ini bertujuan untuk mengetahui frekuensi pemijahan, jumlah telur dan waktu pemijahan populasi induk ikan kerapu tikus (Cromileptes altivelis) di hatchery.  Jumlah induk yang bervariasi antara 20-83 ekor dengan ukuran 1,5-2 kg ditempatkan dalam bak beton bulat, kapasitas 225 m3. Hasil penelitian ini menunjukkan bahwa dengan bertambahnya jumlah induk, maka frekuensi pemijahan yang diperoleh juga semakin meningkat (R2=0,694), dan produksi telur juga semakin meningkat (R2=0,828). Pemijahan kerapu tikus dapat berlangsung setiap bulan, di mana waktu pemijahan terjadi pada kuarter keempat hingga kuarter ketiga. Kata kunci: Biologi reproduksi, Pemijahan, Cromileptes altivelis

Fin Type Variation of Lionhead Strain Goldfish (Carassius auratus) Offspring

Jurnal Akuakultur Indonesia Vol 3, No 3 (2004): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

 Lionhead strain goldfish (Carassius auratus) inheritance produce many variations in phenotype qualitative traits of their offspring that is not common with the parents. Lionhead is an ornamental freshwater fish, they do not have a dorsal fin, but it is a beauty finfish, is popular to the people and have a high economic value. Of the 846 offspring of lionhead is produced in this experiment, and 57,7% of them have dorsal fin (42,3% normal), 13,1% of them have anal fin which did not similar with their parents, 58,6% caudal fin of them did not have similar to their parents. It might be caused by incompletely segregation in meiosis and many gen responsible to certain phenotype trait. Another abnormalities such as no anal fin, blindness, stumped and no pigmen in their gill lamella also occurred. Key words: Inheritance, fin, phenotype, abnormality   ABSTRAK Ikan maskoki strain lionhead menghasilkan keturunan dengan fenotip yang sangat bervariasi dan berbeda dengan induknya. Ikan ini merupakan ikan hias air tawar yang tidak memiliki sirip punggung namun tetap memiliki keindahan, sehingga menjadi begitu populer di masyarakat dan memiliki nilai ekonomi yang tinggi. Sebanyak 846 keturunan (anakan) telah dihasilkan dalam percobaan ini, dan sebanyak 57,7% dari populasi tersebut memiliki sirip punggung (berarti 42,3% merupakan keturunan normal), 13,1% memiliki sirip dubur yang tidak mirip dengan induknya. Variasi keturunan ikan maskoki strain lionhead ini disebabkan oleh segregasi yang tidak sempurna dalam proses meiosis dan banyaknya gen yang terlibat dalam pembentukan penotip tertentu. Abnormalitas lainnya juga terjadi pada keturunan ikan maskoki strain lionhead ini, seperti tidak adanya sirip dubur, mata buta, tubuh pendek dan tidak adanya pigmen pada lemela insang Kata kunci: Keturunan, sirip, fenotip, abnormalitas  

Radio Protective Effects of Ginseng Extract in Gamma-Rays Induced Chromosomal Damages of Human Lymphocyte

Atom Indonesia Vol 34, No 1 (2008): January 2008
Publisher : PPIKSN-BATAN

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Abstract

Ginsan, a polysaccharide extracted from Panax ginseng and subsequently referred as ginseng, posses various biological properties as an anticancer and antioxidant agent. Ginseng also approved effective against radiation effects through its immunomodulating actions in whole body irradiated mouse. But its protective effects on radiation induced DNA damage are not thoroughly investigated, mainly in human. This experiment aimed to assess the effects of ginseng at 2 working doses in suppressing radiation effects of human peripheral blood lymphocyte (PBL) i.e. chromosome aberration and micronuclei yields. The treatment times were 24 hours before, subsequently (0 hour) or 3 hours after and irradiation with gamma rays at doses of 0.5 - 2.0 Gy (dose rate of 3.16 Gy/min). Treated and untreated blood cultivation and metaphase spreading technique was done according to standard procedures. Results showed that without ginseng treatments, radiation significantly increased dicentrics and micronuclei frequencies. Different with the results in mouse study, however, our results indicated that none of the experimental concentrations of ginseng crude water extract tested had an effect on baseline chromosomal aberration and micronuclei (MN) yields in PBL. A protective effect was only seen in chromosome aberration yields of sample irradiated with 2.0 Gy and treated with ginseng 3 h post irradiation rather than 24 h pre-irradiation in one volunteer. Opposite results that ginseng suspected to be a weak radiosensitizer was found in some cases. This may be due to discrepancies exist in route of treatment and its fundamental mechanisms of protective action between both studies. Even though in general it was not effective, the possible mechanism involved in radioprotective influence of ginseng is discussed.

Effectiveness of Gamma Rays in Attenuating Rodent Malaria Parasites of Plasmodium berghei in Blood of Mice

Atom Indonesia Vol 39, No 1 (2013): April 2013
Publisher : PPIKSN-BATAN

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Abstract

Malaria is a major public health problem in Indonesia. Therefore, an effective vaccine against this disease is actively being sought by using gamma rays to attenuate the parasites. However, the safety and efficacy of the resulting vaccine are dependent on the precise irradiation dose. The aim of this research was to determine the exact time when the parasites are attenuated by gamma ray exposure. Mice blood containing Plasmodium berghei of 5,0 X 107 parasites/ml was irradiated with gamma rays at doses of 0, 150, 175 and 200 Gy (doses rate of 380 Gy/h) and then was injected intraperitoneally to mice at 0, 1, 2, 3, and 4 h post irradiation. The parasitemia (parasite density) in mouse blood was observed starting with day 2 and repeated every 2-4 days up to 28 days. The survival of the mice was also observed during the experiment. The results showed that the pre-patent period advanced with exposing infected blood to 150 and 175 Gy irradiations, suggesting some degree of attenuation. The amount of radiation required to render the parasites non-viable is about 175 Gy for an inoculum of a number of parasites, but a delay of 4 h resulted in the death of parasites. There was no difference in the infectivity of irradiated parasite injected 1 h and 2 h post irradiation in terms of parasitemia and the survival of mouse. For a dose of 200 Gy which was injected 2 h post irradiation, no parasitemia was found in the blood and animals which died after times varying from 1 to 4 weeks. We concluded that irradiated parasites should be injected into the host within 1 h after irradiation.Received: 16 January 2013; Revised: 23 April 2013; Accepted: 24 April 2013

Molecular Identification of Mycobacterium tuberculosis and Analysis of Its Resistance to Rifampin in Sputa From Tuberculosis Suspected Patients

Atom Indonesia Vol 36, No 2 (2010): August 2010
Publisher : PPIKSN-BATAN

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Abstract

An accurate identification of different species of Mycobacterium provides to allow appropriate treatment for Mycobacterium tuberculosis infection. Beside that, drug resistance of M. tuberculosis strains to rifampin is not clearly understood in contributing to the spread of tuberculosis in Indonesia. To assess the molecular mechanism of rifampin resistance, a number of clinical specimens of M. tuberculosis were analyzed their molecular nature of a part of the rpoB gene using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) methods. DNA’s extracted from sputum samples were amplified and 32P-labeled by PCR with the specific primers and the product was analyzed their mutation conferring resistance by MDE gel electrophoresis. Of the 70 specimens tested, 57 specimens were positive for M. tuberculosis organism only, three specimens contained a mixture of M. tuberculosis and non tuberculosis mycobacteria (NTM), and 10 specimens were negative approved by Duplex PCR. Of these sixty DNA positive samples (thus the sensitivity of PCR was 85.71%), 5 (8.3%) of them suspected to contain mutations in rpoB which were associated with rifampin resistance. Even though the frequency of mutation was low, the results from our study clearly indicate that the molecular mechanism of rifampin resistance in M. tuberculosis isolates from Indonesia involves alterations in the rpoB gene. Molecular diagnosis by PCR which is fast and easy to perform is useful for early and rapid detection of TB in sputum specimen. Received: 27 February 2009; Revised: 28 August 2010; Accepted: 30 August 2010

The Feasibility of Gamma Irradiation for Developing Malaria Vaccine

Atom Indonesia Vol 37, No 3 (2011): December 2011
Publisher : PPIKSN-BATAN

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Abstract

Malaria, a plasmodial disease, causes more than one million deaths per year and has a significant public health impact. Improved access to prompt treatment with effective antimalarial drugs need to be conducted for prevention of infection in high risk groups. However, the parasite as causal agent has exhibited a potential danger of wide-spread resistances. This warning has directed attention to the study of alternative methods of protection against the disease, among them is to do the immunization. A deeper understanding of the nature and regulation of protective immune mechanisms against this parasite will facilitate the development of much needed vaccines. Developing a malaria vaccine remains an enormous scientific, technical, and financial challenge. Currently a vaccine is not fully available. Among the practical applications of radiobiological techniques that may be of considerable interest for public health is the use of ionizing radiation in the preparation of vaccines. Convincing data were reported that sporozoites of Plasmodium berghei irradiated with X- or gamma-rays, provide an antigenic stimulus effective to induce a protective immune response in mice and rats against subsequent sporozoite infection. Irradiated parasites are better immunogens than killed ones and although non-infective they are still metabolically active, as shown by continued protein and nucleic acid synthesis. There is a substantial number of data from human studies demonstrating that sporozoites attenuated by radiation are potent inducers of protective immunity and that they are safe and do not give rise to the asexual erythrocytic infections that cause malaria. This vaccine is relatively inexpensive to produce, easy to store, and transportable without refrigeration. A long-term effort and commitment to providing resources must be maintained and increased to achieve the goal of a malaria vaccine candidate where ionizing radiation as a tool to prepare is seemingly feasible. Received: 20 November 2010; Revised: 11 July 2011; Accepted: 01 August 2011

PENYELESAIAN PERSAMAAN SCHRÖDINGER POTENSIAL NON-SENTRAL SCARF HIPERBOLIK PLUS ROSEN-MORSE TRIGONOMETRIK MENGGUNAKAN METODE SUPERSIMETRI MEKANIKA KUANTUM

Jurnal Spektra Vol 16, No 2 (2015): Spektra: Jurnal Fisika dan Aplikasinya
Publisher : Jurnal Spektra

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Abstract

AbstrakTujuan dari penelitian ini adalah menyelesaikan persamaan Schrödinger potensial non-sentral Scarf hiperbolik plus Rosen-Morse trigonometrik menggunakan metode Supersimetri Mekanika Kuantum (SUSI MK).Spektrum energi dan fingsi radial diperoleh dari penyelesaian persamaan Schrödinger bagian radial, sedangkan fungsi gelombang bagian sudut dan bilangan kuntum orbital diperoleh dari persamaan Schrödinger bagian sudut polar.Spektrum energi dan bilangan kuantum orbital ditentukan dengan sifat shape invariance.Penentuan fungsi gelombang tingkat dasar bagian radial ditentukan dengan sifat lowering operator dan fungsi gelombang tereksitasi ditentukan dengan sifat raising operator.Baik untuk bagian radial maupun bagian polar ditentukan dengan menggunakan sifat lowering operator dan raising operator. AbstractThe purpose of this research is to solve the Schrödinger equation of non-central potential Scarf hyperbolic plus Rosen-Morse trigonometric method Supersymmetry Quantum Mechanics (SUSY QM). Energy spectrum and radial functions derived from the completion of the radial part of the Schrödinger equation, while the wave functions of the corners and orbital quantum numbers obtained from the Schrödinger equation part of the polar angle. Energy spectrum and orbital quantum number is determined by the nature of the shape invariance. Determination of the wave functions of the radial part of the base rate is determined by the nature of lowering operators and the excited wave function is determined by the nature of the raising operator. Both for the radial and polar parts determined by using the properties of lowering and raising operators.Keywords: Schrödinger equation, the non-central potential Scarf hiperbolic plus Rosen-Morse trigonometric, Supersymmetry Quantum Mechanics

Relation between broodstock number and spawning frequency and egg production of humpback grouper (Cromileptes altivelis)

Jurnal Akuakultur Indonesia Vol 6, No 2 (2007): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Original Source | Check in Google Scholar | Full PDF (166.057 KB)

Abstract

This study was performed to determine spawning frequency, number of ovulated egg and spawning time of humpback grouper (Cromileptes altivelis) broodstock in hatchery.  Broodstock of 20-83 fish in weight of 1.5-2.0 kg were reared in circular concrete tank 225 m3.  The results showed that increasing in number of broodstock increases spawning frequency (R2= 0.694), and ovulated eggs number was also increased (R2= 0.828).  Spawning of humpback grouper can occur in the third to fourth week in every month. Keywords: reproductive biology, spawning, Cromileptes altivelis   ABSTRAK Penelitian ini bertujuan untuk mengetahui frekuensi pemijahan, jumlah telur dan waktu pemijahan populasi induk ikan kerapu tikus (Cromileptes altivelis) di hatchery.  Jumlah induk yang bervariasi antara 20-83 ekor dengan ukuran 1,5-2 kg ditempatkan dalam bak beton bulat, kapasitas 225 m3. Hasil penelitian ini menunjukkan bahwa dengan bertambahnya jumlah induk, maka frekuensi pemijahan yang diperoleh juga semakin meningkat (R2=0,694), dan produksi telur juga semakin meningkat (R2=0,828). Pemijahan kerapu tikus dapat berlangsung setiap bulan, di mana waktu pemijahan terjadi pada kuarter keempat hingga kuarter ketiga. Kata kunci: Biologi reproduksi, Pemijahan, Cromileptes altivelis

Assessment of Individual Radiosensitivity in Inhabitants of Takandeang Village - A High Background Radiation Area in Indonesia

Atom Indonesia Vol 45, No 1 (2019): April 2019
Publisher : PPIKSN-BATAN

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Abstract

People living in high background radiation area (HBRA) possibly developed the radioadaptive response (RAR) phenomena. Mamuju area in West Sulawesi Indonesia also known as the HBRA in Indonesia due to high natural uranium contents. It is possible that RAR was developed in Mamuju inhabitants. To prove this hypothesis, here in this study evaluation of the individual radiosensitivity in Takandeang Village, Mamuju inhabitants using G2 micronucleus (MN) assay was conducted. Association between blood groups and TP53 Arg72Pro polymorphism with individual radiosensitivity also evaluated in this study. Using G2 MN assay we assessed the individual radiosensitivity of Takandeang Village inhabitants and control samples. For each sample, three parameters were calculated. The spontaneous (baseline) MN number, MN number after 0.5 Gy in vitro irradiation and radiation induced MN were calculated to predict the individual radiosensitivity. The radiation induced MN was defined by subtracting the spontaneous MN number from the MN number after irradiation. The mean and SD of the number of micronucleus induced by radiation found in control group (CG) was set as the cut-off value to determine the individual radiosensitivity in all samples. The radiation induced MN higher than the mean CG + 1SD CS was scored as 1, indicating a milder radiosensitive phenotype, whereas a result higher than the mean CG + 2SD CG was scored as 2, and indicated a more severe radiosensitive phenotype. When the individual value was lower than the mean CG + 1SD CG, a score of 0 was attributed to the tested subject. Results showed that four individuals in Takandeang Village inhabitants were having a milder radiosensitive phenotype, whilst the other was categorized as normal radiosensitive. The similar finding also found in control samples. Our study failed to find a correlation between radiosensitivity and blood group also the TP53 Arg72Pro polymorphism. Overall our study revealed the possibility of RAR phenomena in Takandeang Village inhabitants. Further investigation using a different point of radiation dose value and larger sample number should be performed to validate this study results.        

KELANGSUNGAN HIDUP DAN PERTUMBUHAN BENIH IKAN LELE (Clarias sp.) PADA SALINITAS MEDIA YANG BERBEDA

Jurnal Akuakultur Rawa Indonesia Vol 5, No 1 (2017): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

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Abstract

ABSTRACT The research consisted of two phases, pre research and research phase. Pre research was carrried out to quantify the isoosmotic of Juvenile catfish by conductivity both on media and fish body which purpose to make  the curve of the correlation between salinity with conductivity. The research phase was the maintenance of Juvenile Catfish for 20 days to prove isoosmotic value to survival rate, growth and feed conversion. This research used a completely randomized design with 5 treatments and 3 replications. The treatments were media freshwater (control), 2 ppt (P1), 4 ppt (P2), 6 ppt (P3), 8 ppt (P4). The research value showed there was positive correlation between salinity and conductivity. The isoosmotic value for Juvenil Catfish was 5,893 mS/cm or 4,11 ppt salinity. Survival, growth and feed conversion ratio shows the results were not significantly different (P <0,05). Meanwhile, regression equation indicated salinity (X) that optimal for the maintenance of Juvenile Catfish ranging from 2,6 to 5,0 ppt. Keywords : Conductivity, Growth, Isoosmotic, Salinity, Survival rate