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Monitoring Salmonella sp dan Escherichia coli dalam Bahan Pakan Ternak (Salmonella sp and Escherichia coli Monitoring in the Animal Feed Ingredients) Suwito, Widodo
Buletin Peternakan Vol 34, No 3 (2010): Buletin Peternakan Vol. 34 (3) Oktober 2010
Publisher : Fakultas Peternakan UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.515 KB) | http://journal.ugm.ac.id/index.php/buletinpeternakan/article/view/86

Abstract

Quality of livestock feed affects production and animal health. Fish and bone meal is animal feed ingredients that need to be monitored because highly contaminated with of Salmonella sp and E. coli. Salmonella sp and E. coli arepathogen causing diarrhea in cattle and humans. The aim of this study was to investigate both E. coli and Salmonella contamination animal feed ingredients. A total of 38 animal feed ingredients samples from a poultry farm in Sukabumiconsisting of 15 samples fish meal, bone meal 13 samples and corn 10 samples. These samples were analyzed for Salmonella sp and E. coli by isolation and identification using biochemical analysis. Salmonella Weltevreden wasisolated from fish meal at rates of 6% from the 15 samples. E. coli was isolated at rates of 6% from the 15 fish meal samples and 7% from 13 bone meal samples, while corn samples were free from Salmonella sp and E. coli. This studyshowed that bone meal and fish meal was contaminated slightly by Salmonella Weltevreden and E. coli.(Key words: Feed, Livestock, Salmonella sp, E. coli)
DETEKSI Escherichia coli 0157:H7 DARI SUSU DAN DAGING MENGGUNAKAN SERUM KEBAL MONOSPESIFIK Suwito, Widodo
Jurnal Sain Veteriner Vol 28, No 2 (2010)
Publisher : Fakultas Kedokteran Hewan

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Abstract

Escherichia coli 0157:H7 merupakan salah satu bakteri patogen penyebab food borne disease pada manusia. Deteksi E.coli 0157:H7 pada serum keba] monospesifik berdasarkan somatik antigen (0) dan flagella(H). Tujuan dari penelitian ini untuk mendeteksi E.coli 0157:H7 menggunakan serum kebal monospesifik dengan cara absorbsi dan uji koaglutinasi. Serum kebal monospesifik diperoleh dengan cara menyuntikkan antigen E.coli 0] 57:H7 pada kelinci jenis New Zealand White. Serum dikoleksi dan diabsorbsi menggunakan antigen somatik E.coli heterolog sampai tidak terjadi reaksi agglutinasi. Hasil penelitian menunjukkan bahwa dengan serum kebal monospesifik dapat digunakan untuk menedeteksi E.coli 0 157:H7 dalam susu dan daging.Kata kunci: serum keba1, monospesifik, E.coli 0157:H7
ISOLATION AND IDENTIFICATION BACTERIA OF CLINICAL MASTITIS ON ETTAWAH CROSSBRED GOAT Suwito, Widodo; Wahyuni, A.E.T.H.; Nugroho, Widagdo Sri; Sumiarto, Bambang
Jurnal Sain Veteriner Vol 31, No 1 (2013)
Publisher : Fakultas Kedokteran Hewan

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Abstract

ABSTACT Ettawah crossbred goat (PE) IS much more usefull to milk goat produce. One of constraints in milk goat is clinical mastitis. Clinical mastitis is economic inflict a financial lost because stopped of milk production, high cost of treatment, and even cause death. The aim of the present study was to determine the kind bacteria that can cause clinical mastitis in PE. A total of 13 clinical mastitis milk and water used to wash the six udder samples were collected from dairy PE farms. All samples were subjected  for isolation and identification of bacteria based on biochemical reactions. From the clinical mastitis milk samples, bacteria isolated were Staphylococcus aureus (55.55%), Pseudomonas sp (27.77%), Streptococcus sp (8.3%) and Bacillus sp (8.33%). Whereas, Pseudomonas sp (77.77%) and Bacillus sp (22.23%) were isolated from the water used to wash the udder. The study showed that more clinical mastitis in PE caused by Staphylococcus aureus. Water use to wash the udder was source of Pseudomonas sp causing clinical mastitis in PE.  
Isolasi and Identification of Bacteria from the Urine Fluid Organic Fertilizer (POC)Ettawa Crossbred (PE) in The Sleman Regency Suwito, Widodo; Wahyuni, A.E.T.H.; Nugroho, Widagdo Sri; Sumiarto, Bambang; Bekti, Utomo Bimo
Jurnal Sain Veteriner Vol 31, No 2 (2013)
Publisher : Fakultas Kedokteran Hewan

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Abstract

Urine is a by product from Ettawa crossbred (PE) goat farm in Sleman regency. In addition, the manure urine goats can be used for fluid organic fertilizer (POC). Recently, organic farming is more interesting for people because it does not use any chemical fertilizers. Salmonella sp and E. coli O157:H7 are pathogenic bacteria that can contaminate agricultural products and dangerous for public health. One of sources of contamination in agricultural products is due to the organic fertilizer. Therefore, the aim of the present study was to determine pathogenic bacteria from POC urine PE goats in Sleman regency. A total of 8 POC samples were collected from PE goat urine in Sleman regency. These samples were analyzed for Salmonella sp, E. coli O157:H7 and other pathogenic bacteria by using biochemical reactions. All samples are negative for Salmonella sp. and E. coli O157:H7 as well. Whereas, 85% of 8 samples are positive for Pseudomonas sp. The present study showed that POC from PE goats farm in Sleman regency are negative for pathogenic bacteria.  
Faktor-Faktor Risiko Mastitis Subklinis pada Kambing Peranakan Etawah di Kabupaten Sleman, Yogyakarta (RISK FACTORS OF SUBCLINICAL MASTITIS ON ETTAWA CROSSBRED GOAT IN SLEMAN REGENCY, YOGYAKARTA) Suwito, Widodo; Nugroho, Widagdo Sri; Sumiarto, Bambang; Wahyuni, Agnesia Endang Tri Hastuti
Jurnal Veteriner Vol 15, No 1 (2014)
Publisher : Jurnal Veteriner

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Abstract

In Sleman, a regency in Yogyakarta special region, Etawah crossbred goats are excessively bred for thedairy produce called the goat’s milk. Subclinical mastitis is one of diseases which reduce the yield of goat’smilk. The aim of this study was to determine the risk factors that contribute to the subclinical mastitis onthe Etawah crossbred goats in Sleman. The 200 samples one of which contains 10 mL of goat’s milk weretaken from the udders of the Etawah crossbred goats from the eight goat farms in Sleman. The 200samples were analyzed for the subclinical mastitis using California Mastitis Test (CMT). The data of riskfactors were gathered through a questionnaire. The risk factors on Etawah crossbred goats in Sleman weredetermine with the use of bivariate analysis chi square (X)2, odds ratio (OR) and relative risk (RR). Thegoat’s milk which subclinical mastitis was isolation and identification of bacteria based on biochemicaltests. The risk factors that cause the subclinicall mastitis on Etawah crossbred goats in Sleman were (1)milk yield (X2=14.23; OR=6.52; RR=4.42), (2) age status of lactation (X2=1.60; OR=59.09; RR=17.94), (3)age of weaning (X2=26.06; OR=2.22; RR=1.91), and (4) Body Condition Score (BCS) (X2=13.89; OR=1.29;RR=1.22). Goat’s milk which subclinicall mastitis were isolated  Bacillus sp  (70%), Staphylococcus sp(33%), Pseudomonas sp (29%), Streptococcus sp (25%), Corynebacterium sp (12%), and E. coli (4%).
Determinasi Salmonella sp dan Ektoparasit dalam Pupuk Organik dari Kotoran Sapi Potong di Yogyakarta Suwito, Widodo; -, Supriadi; Winarti, Erna; Bimo Bekti, Utomo
Biota Biota Volume 13 Nomor 2 Tahun 2014
Publisher : PBI Yogyakarta

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Abstract

AbstractOne of by products derived from beef cattle farm is manure. The manure can be processed to make organic fertilizer. Salmonella sp is bacterium of the manure origin and harmful for human. Currently, organic fertilizer is mostly used for vegetables and fruits, but the problem is people consume those vegetables. The aim of this study was to determine Salmonella sp and ectoparasite in organic fertilizer which were made from the manure of beef cattle farm in Yogyakarta. A total of 10 manure samples were collected the farms to make organic fertilizer. The manure was fermented with lactic acid bacteria (BAL) for one month. Before and after fermented, the manure was isolated and identified for Salmonella sp based on biochemical reactions and ectoparasite using native method. The study showed that there were no Salmonella sp and ectoparasite in the organic fertilizer made from the manure fermented for one month.Keywords: Organic fertilizer, manure, farm, Salmonella spAbstrakSalah satu hasil sampingan dari peternakan sapi potong adalah kotoran ternak. Kotoran ternak dapat dimanfaatkan untuk dibuat pupuk organik. Salmonella sp merupakan bakteri yang berasal dari kotoran ternak dan dapat membahayakan kesehatan manusia. Saat ini pupuk organik banyak digunakan untuk tanaman sayuran dan buah-buahan, sedangkan sebagian masyarakat mengonsumsi sayuran dalam keadaan mentah. Tujuan dari penelitian ini adalah untuk mengetahui Salmonella sp dan ektoparasit dalam pupuk organik yang dibuat dari kotoran sapi pada peternakan sapi potong di Yogyakarta. Telah dikumpulkan sebanyak 10 sampel kotoran sapi yang akan dibuat pupuk organik dari peternakan sapi potong di Yogyakarta. Kotoran sapi difermentasi dengan bakteri asam laktat (BAL) selama satu bulan. Kotoran sapi sebelum dan sesudah difermentasi dilakukan isolasi dan identifikasi Salmonella sp berdasarkan reaksi biokimia dan ektoparasit dengan metode natif. Penelitian ini menunjukkan bahwa di dalam kotoran sapi yang dibuat pupuk organik dengan fermentasi selama satu bulan tidak ditemukan Salmonella sp.Kata kunci: Pupuk organik, rabuk, peternakan sapi, Salmonella sp
ANALISIS MIKROBIOLOGI SUSU KAMBING PERANAKAN ETTAWA (PE) DARI KABUPATEN SLEMAN YOGYAKARTA Suwito, Widodo; Sri Nugroho, Widagdo; Wahyuni, AETH Wahyuni; Sumiarto, Bambang
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.189 KB) | DOI: 10.21157/j.ked.hewan.v8i2.2625

Abstract

Tujuan penelitian ini mengetahui kualitas mikrobiologi susu kambing mentah yang diambil langsung dari ambing. Dalam penelitian ini digunakan 50 sampel susu kambing peranakan Ettawa (PE). Sampel dianalisis terhadap total plate count (TPC), jumlah Staphylococcus sp., jumlah koliform, Escherichia coli (E. coli), dan Salmonella sp. berdasarkan reaksi biokimia. Rerata untuk TPC; Staphylococcus sp.; total koliform masing-masing adalah 1,65x10 3 ; 5,75x10 3 ; 1,3x10 cfu/ml, sedangkan E. coli dan Salmonella sp. adalah negatif. Berdasarkan Standar Nasional Indonesia (SNI) No 01-6366-2000 tentang persyaratan susu segar, maka TPC, koliform, E. coli, dan Salmonella sp. memenuhi standar, sedangkan Staphylococcus sp. melebihi ambang batas. Hasil penelitian menunjukkan bahwa susu kambing mentah yang diambil langsung dari ambing masih layak konsumsi.
Impacts of Verotoxigenic and Enterohaemorrhagic Escherichia Coli (Vtec and Ehec) on Animal, Human and Food Suwito, Widodo
Indonesian Bulletin of Animal and Veterinary Sciences Vol 19, No 2 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.554 KB) | DOI: 10.14334/wartazoa.v19i2.922

Abstract

Verotoxigenic and enterohaemorrhagic Escherichia coli, VTEC and EHEC are strains that produce a toxin on the Vero cells in vitro, therefore the toxin called verotoxin. Strain VTEC and EHEC have been isolated from human and various animal species, mainly ruminants and pigs. The prevalence of VTEC in cattle is 35%, milk 10%, cheese 1.5%. In beef meat, pork, poultry, goat and sheep is 3.7, 1.5, 1.5, 2 and 2.5%, respectively. In contrast, the human prevalence in United States, Spain and France 5 – 10, 2.5 and 3.4%, respectively. In Indonesia, nine cases of VTEC were reported by Ciptomangunkusumo Hospital and four out of nine cases were died. The verotoxin also named Shiga toxins (Stx), is active in vivo as a pathogen on the endothelial cells of the blood vessels of the gastro intestinal mucosa, kidneys, brain and other tissues of human and piglets. Verotoxin in human, ruminants and piglets causes bloody diarrhea so with ruminants. In the adult ruminants, verotoxin results in clinical signs and they act as asymptomatic carriers of VTEC and EHEC strains but in human, it produced haemorrhagic colitis (HC), haemolytic uremic syndrome (HUS) and thrombocytopenia purpura (TPP). Infection in human by the most famous EHEC strain belongs to the O157:H7 serotype, through faecal contamination or environment of either food of animal origin, or other foodstuffs (fruits and vegetables). Infection from EHEC strains could be prevented by avoiding food of animal origin and unpasteurized milk, and by taking care of food hygiene for comsumption.   Key words: verotoxin, enterohaemorrhagic, VTEC, EHEC, Shiga toxin (stx), ruminant
Mastitis in Ettawa Crossbred Goat (Pe) Caused by Staphylococcus Aureus: Epidemiology, Clinical Signs, Pathogenesis, Diagnosis and Control Suwito, Widodo; S, Indarjulianto
Indonesian Bulletin of Animal and Veterinary Sciences Vol 23, No 1 (2013)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.598 KB) | DOI: 10.14334/wartazoa.v23i1.953

Abstract

Mastitis in Ettawa crossbred goat is commonly found and caused economic loss. Staphylococcus aureus is one of bacteria caused clinical mastitis or subclinical mastitis in Ettawa crossbred goat. The aim of this review was to provide an overview of clinical and subclinical mastitis in the Ettawa crossbred goat caused by S. aureus from epidemiological aspect, clinical symptoms, pathogenesis, diagnosis, treatment, prevention and control. Mastitis should be eliminated because it lead to death for the goats and lambs. In addition, S. aureus has greater risk for contamination in milk because it produces heat-stable toxin. Isolation and identification bacteria with total of somatic cell counts are important as a reference to determine the actions to decrease the occurrence of mastitis. Some preventive measures for mastitis include clean milking, dipping the teats with a disinfectant and antibiotic treatment during dry lactation. Key words: Ettawa crossbred goat, mastitis, S. aureus, diagnosis, preventif
Verotoxigenic Escherichia coli (VTEC) isolated from cow milk Suwito, Widodo
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 3 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (183.713 KB) | DOI: 10.14334/jitv.v14i3.346

Abstract

Verotoxigenic Escherichia coli (VTEC) strains are responsible for serious human illnesses. These strains are commonly found in milk. The aim of this study was to determine the occurrence of verotoxigenic E. coli in milk. A total of 351 milk samples, were collected from dairy farms in Bogor, Sukabumi and Cianjur. These samples were analyzed for VTEC using biochemical, serological and vero cell cytotoxicity assays. VTEC O157:H7 isolates were found in milk collected from dairy herds in Bogor and Sukabumi at rates 0.47% of 214 samples, 1.10% of 91 samples respectively, and none in Cianjur. Hemolytic E. coli isolates were found in 0.94% of 214 milk samples from Bogor, 2.2% of 91 milk samples from Sukabumi and none from Cianjur. From E. coli isolates, 53 isolates (67.95%) were verotoxigenic, consisted of: two E. coli O157:H7 isolates and 51 non O157:H7 isolates.Therefore this study showed the occurrence of VTEC in milk samples from dairy farms in Bogor, Sukabumi and Cianjur. Key words: Milk, E. coli (VTEC) O157:H7, Verotoxigenic