Ni Ketut Suwiti
Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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FENOMENA JEMBRANA DISEASE DAN BOVINE IMMUNODEFICIENCY VIRUS PADA SAPI BALI Suwiti, Ni Ketut
Buletin Veteriner Udayana Vol. 1 No. 1 Pebruari 2009
Publisher : Buletin Veteriner Udayana

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Abstract

Sapi bali(Bos sondaicus) merupakan salah satu ternak unggulan sebagai penghasil daging di Indonesia yang harus terus dikembangkan dan ditingkatkan populasinya. Dari tahun ke tahun permintaan akan daging semakin meningkat, data Bappenas menunjukkan untuk memenuhi kebutuhan daging nasional,pada tahun 2002-2003 telah diimpor sapi bakalan dari Australia sebanyak 325.000 – 375.000, sedangkan impor daging pertahun setara dengan 100.000 ekor sapi (Apfindo 2005).Berdasarkan data tersebut sapi bali sangat berpeluangdikembangkan secara makimum untuk memenuhi kebutuhan daging di IndonesiaFenomena adanya dua bovine lentivirus pada sapi bali yakni Jembrana Disease Virus danBovine Immunodeficiency Virus, segera harus dibuktikan keberadaannya. Selanjutnya dapat dikembangkan penelitian yang bertujuan kearah produksi vaksin heterolog untuk mencegah penyakit jembrana, danakhirnya membantu peningkatan populasi sapi bali.
DETEKSI HISTOLOGIK KESEMBUHAN LUKA PADA KULIT PASCA PEMBERIAN DAUN MENGKUDU (Morinda Citrofilia Linn) Suwiti, Ni Ketut
Buletin Veteriner Udayana Vol 2 No. 1 Pebruari 2010
Publisher : Buletin Veteriner Udayana

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Abstract

A study to detect the microscopic structure of skin wound in mice (Mus musculus) aftertreatment with mengkudu leaves (Morinda citrifolia Linn.), by histological methods, hasbeen carried out. In this study were detected the level of wound healing every weeks. Thesamples were collected from skin with was given incision injury. The tissue samples werefixed, dehydrated and embedded in paraffin and 4 – 5 ?. sections. Harris-Haematoxilin-Eosin staining method, using to identified of histological structure.Microscopic analysiswas performed using a binocular light microscope (450X). The study showed that,histological structure of normaly skin we deteted in fourth weeks ware composed byepidermis, dermis and hipodermis tissue. We observed for the presence ofthe ephitelialsquomous complexs, hairfollicles, connective tissue with vein and arteriole and adiposetissue.
STUDI HISTOLOGI LIMPA SAPI BALI Setiasih, Ni Luh Eka; Suwiti, Ni Ketut; Suastika, I Putu; Piraksa, I Wayan; Susari, Ni Nyoman Werdi
Buletin Veteriner Udayana Vol. 3 No. 1 Pebruari 2011
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The objective of this study to find out the histological of spleen of bali cattle. Thespleenwere collected from 20 bali cattle in Pesanggaran abattoir, Denpasar and thenevaluated microscopically. Histological findings were assessed by H-E stainpreparations.Microscopically, showed that the spleen of bali cattle consists of capsule,white and red pulp. The capsule composed of connective tissue and smooth muscle withthick of these layer is 24,3 ± 3,7 ?m. The white pulp subdivided into the marginal zone andthe follicle. It is composed of reticulocyte (lymphocytes and macrophages) andreticular fibers. The red pulp composed of arterioles, capillary, and vascular sinusoidswhich are large numbers of erythrocytes, macrophages, dendritic cells, plasma cells andsparse lymphocytes.
Pengaruh Pemberian Pegagan (Centella asiatica) terhadap Struktur Mikroskopis Hati Mencit Pasca Infeksi Salmonella typhi Bhirawa, Brahma Tusta; Suwiti, Ni Ketut; Setiasih, Ni Luh Eka; Besung, I Nengah Kerta
Buletin Veteriner Udayana Vol. 4 No.2 Agustus 2012
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Abstract

Centella asiatica (pegagan) merupakan salah satu tanaman obat yang memilikibanyak fungsi, salah satunya adalah sebagai imunostimulator, yang berarti dapatmerangsang tubuh untuk meningkatkan respon imun. Namun efek pegagan terhadapstruktur mikroskopis hati pasca diinfeksi Salmonella typhi (S.typhi) belum pernahdilakukan. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak pegagan terhadapstruktur mikroskopis hati mencit yang diinfeksi S.typhi . Penelitian ini menggunakan 24ekor mencit jantan umur 8-12 minggu, yang dibagi menjadi 4 grup. P0 sebagai kontroldiberikan aquades, dan grup P1,P2, dan P3, masing-masing diberikan 125 mg/bb, 250kg/bb mg, 500 mg kg/bb. perlakuan diulang sebanyak 6 kali. Extrak pegagan diberikansecara oral selama 14 hari, pada hari ke 15 seluruh mencit diinfeksi dengan S.typhi,kemudian pada hari ke 14 semua mencit dinekropsi dan hati diambil untuk diprosespembuatan preparat dengan metode pewarnaan hematoxylin eosin (HE). Pengamatandilakukan terhadap gambaran mikroskopis berupa perdarahan, degenerasi vakuola, dannekrosis. Hasil analisis menunjukkan mencit yang tidak diberikan pegagan dengan mencityang diberikan pegagan dosis 125 mg/kg bb., memberikan hasil yang tidak berbeda nyataNamun mencit yang diberikan pegagan dosis 250 mg/kg bb, dan 500 mg/kg bb memberikangambaran mikroskopis yang berbeda nyata.
IDENTIFIKASI DAGING SAPI BALI DENGAN METODE HISTOLOGIS Suwiti, Ni Ketut
Majalah Ilmiah Peternakan Vol 11, No 1 (2008)
Publisher : Majalah Ilmiah Peternakan

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ABSTRAK Telah dilakukan penelitian identifikasi daging sapi bali dengan metode histologis yakni melakukan pengamatan terhadap struktur mikroskopis. Sampel daging sapi bali. berupa muskulus pectoralis profundus, diperoleh dari pasar swalayan di Denpasar. Sampel difiksasi, didehidrasi dan diembedding dalam parafin selanjutnya dilakukan pemotongan dengan mikrotom ketebalan 4 - 5 µ. Dibuat sediaan histologis dengan metode pewarnaan Harris-Haematoxilin-Eosin. Pengamatan terhadap struktur histologi dilakukan dengan mikroskop cahaya binokuler pembesaran 450x. Hasil penelitian menunjukkan struktur histologi daging sapi bali terdiri atas serabut otot skelet longitudinal dan tranversal dengan multinuklleus. Nukleus terletak dipinggir sel. Ditemukan jaringan ikat padat dan jaringan lemak dengan pembuluh darah. Diameter serabut otot berukuran 8,40 ± 1,41 µm. Pada pengamatan histologis juga ditemukan : endomisium, perimisium dan epimisium IDENTIFICATION OF BALI CATTLE MEAT WITH HISTOLOGICAL METHODS ABSTRACT A study to identification the microscopic structure of bali cattle meat by histological methods, has been carried out. The meat bali cattle samples were collected from musculus pectoralis profundus has been taken from Denpasar supermarket. The tissue samples were fixed, dehydrated and embedded in paraffin and 4 - 5 µ. sections. Harris-Haematoxilin-Eosin staining method, using to identified of histological structure. Microscopic analysis was performed using a binocular light microscope (450X). The study showed that, histological structure of bali cattle beef was composed by longitudinally and transverselly skeletal muscle with a multinucleated. The nuclei in the periphery of the cell, there are dense connective tissue, fat, with small blood vessels. The skeletal myofibers diameter of muscle is 8.40 ± 1,41 µm. We observed for the presence of : endomysium , perimysium and epimysium.
Deteksi Protein Bovine Major Histocompatibility Complex Klas I dan Klas II pada Sapi Madura Suwiti, Ni Ketut; Abdul Rantam, Fedik; Kerta Besung, I Nengah
Jurnal Veteriner Vol 9, No 3 (2008)
Publisher : Jurnal Veteriner

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Abstract

A study to detect the presence of bovine major histocompatibility complex (Bo-MHC) in maduracattle has been carried out. Lymphocyte samples were collected from 25 cattle. The protein of lymphocytesamples was analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) andtransferred onto nitrocellulose membrane. Bo-MHC proteins on the membrane were detected by monoclonalantibodies (anti-Bo-MHC class I) and BAQ150A (anti-Bo-MHC class II). The proteins of 48 kDa and 11kDa were detected by MAb B5C which appeared to be the alpha and beta chain of Bo-MHC class I. MAbBAQ150A detected protein band of 26 KDa which was likely to be the beta chain of Bo-MHC class II. Bo-MHC class I dan class II were clearly detected in madura cattle by MAbs which provides a basis for furtherinvestigation on the role of the MHC in the susceptibility the animals to many diseases.
STRUKTUR HISTOLOGI DUODENUM, JEJENUM, DAN ILEUM SAPI BALI Werdi Susari, Ni Nyoman; Eka Setiasih, Ni Luh; Suwiti, Ni Ketut
Jurnal Veteriner Vol 10, No 1 (2009)
Publisher : Jurnal Veteriner

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A study to find out the histological structures of small intestine (ie. duodenum, jejunum, and ileum) of bali cattle have been carried out. A total of 20 small intestines were collected from the abattoir at Pesanggaran, Denpasar Bali. Histological examinations were performed on Haematoxyline Eosin stained small intestines slides. The duodenum, jejunum and ileum of the small intestines have four mucosa membranes: i) mucosa membrane, ii) sub mucosa membrane, iii) muscular membrane and, iv) serous membrane. The average thickness of mucosa membrane of duodenum, jejunum and ileum was 36,3±13,5 mm; 29,9±3,1 mm; and 38,8±11,9 mm, respectively. Each part has villi with an average length of 27,5±8,3 mm; 20,1±3,7 mm; and 18,5±4,3 mm, respectively. This mucosa membranes consist of single columnar epithelium, muscularis mucosa, and lamina propria. The average thickness of sub mucosa membrane was 47,3±15,3 mm; 10,4±2,6 mm; and 16,9±5,6 mm, respectively. Sub mucosa membrane consisted of connective tissue. The average thickness of muscular membrane was 46,9±8,8 mm; 28,1±5,1 mm; and 62,4±11,3 mm, respectively, which consisted of circular and longitudinal smooth muscle. The average thickness of serous membrane was 19,9±3,1 mm; 11,9 ±3,0 mm; and 12,1±3,6 mm, respectively, which consisted of mainly connective tissue. Goblet cells were seen through all the epithel of small intestines with the highest number seen in the ileum. Specific structure of duodenum, jejunum, and ileum which is Brunner’s glands in the sub mucosa membranes of duodenum, circular plicae in the jejunum and mesenteric gland in the ileum were also observed.
STUDI HISTOLOGI USUS BESAR SAPI BALI Suwiti, Ni Ketut; Setiasih, Ni Luh Eka; Suastika, I Putu; Piraksa, I Wayan; Susari, Ni Nyoman Werdi
Buletin Veteriner Udayana Vol. 2 No. 2 Agustus 2010
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Abstract

A Study to detect the microscopic structure of large intestine (caecum, colon, rectum) ofbali cattle has been carried out. The samples were collected from 20 of bali cattle in Pesanggaran abattoir, Denpasar. The tissue samples were fixed, dehydrated and embeddedin paraffin and 4-5 mm sections. Harris-Haematoxilin-Eosin staining method, using toidentified of histological structure. Microscopic analysis was performed using binocularlight microscope (100 x, 450x). The study showed that the wall of the caecum, colon, andrectum are made up of four layers : mucosae, submucosae, muscularis externa, and serosa.The lined by mucosae ephitelium of simple columnar cells. The thick of mucosaare 29,9 ± 5,0 mm (caecum) 37,5 ± 8,4 mm (colon) and 58,9 ± 9,5 mm (rectum)respectively. The submucosae contain connective tissue and thick of these layerare 54,8 ± 8,8 mm; 21,2 ± 7,1 mm; and 16,5 ± 4,5 mm respectively. The muscularis externaconsists of two layers of smooth muscle inner circular and outer longitudinal. The thick ofthe mucularis externa are 98,5 ± 15,4 mm; 166,9 ± 44,4 mm; and 479 ± 28,6 mmrespectively. The serosa forms the outermost layer with thick 29,5 ± 8,5 mm;20,9 ± 7,6mm; and 12,1 ± 3,6 mm respectively. We observed for the presence richof Goblet cells and few limphatic nodules (Peyer’s patches) but no showed villi.
Pengaruh Pemberian Pegagan (Centella asiatica) terhadap Gambaran Mikroskopis Limpa Mencit yang Diinfeksi Salmonella typhi Budiawan, I Gede Oka; Suwiti, Ni Ketut; Suastika, I Putu; Besung, I Nengah Kerta
Buletin Veteriner Udayana Vol. 5 No.1 Pebruari 2013
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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak pegagan terhadap gambaran histologis limpa mencit yang diinfeksi Salmonella typhi. Mencit sebanyak 24 ekor dibagi empat kelompok, yakni kelompok I (P0) sebagai kontrol diberikan aquades steril, kelompok II (P1) diberikan pegagan dengan dosis 125 mg/kg bb, kelompok III (P2) diberikan pegagan 250 mg/kg bb, dan kelompok IV (P3) diberikan pegagan 500 mg/kg bb. Setiap perlakuan diulang sebanyak 6 kali. Pegagan di berikan setiap hari selama 14 hari. Setelah 14 hari seluruh mencit diinfeksi S.typhi. Pada hari ke-15 dilakukan nekropsi untuk pengambilan sampel berupa limpa dan dibuat preparat histologi. Pengamatan preparat dilakukan di Laboratorium Histologi, meliputi persentase nekrosis. Metote pewarnaan menggunakan Haematoxylin Eosin (HE). Data yang diperoleh dianalisis dengan Uji Kruskal-Wallis. Hasil analisis menunjukan gambaran  mikroskopis limpa mencit yang diberikan pegagan dosis 500 mg/kg bb berbeda nyata (P<0,05) dengan limpa mencit yang diberikan pegagan dosis 125 mg/kg bb, 250 mg/kg bb dan mencit yang tidak diberikan pegagan.  
Pengaruh Pemberian Pegagan (Centella asiatica) Terhadap Gambaran Mikroskopis Ginjal Mencit yang Diinfeksi Salmonella typhi Hendrayana, Komang Ariya; Suwiti, Ni Ketut; Kardena, I Made; Besung, I Nengah Kerta
Buletin Veteriner Udayana Vol. 5 No.1 Pebruari 2013
Publisher : Buletin Veteriner Udayana

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Abstract

Telah dilakukan penelitian mengenai pengaruh pemberian pegagan (Centella asiatica) terhadap gambaran mikroskopis ginjal mencit yang telah diinfeksi  Salmonella typhi. Penelitian menggunakan 24 ekor  mencit jantan berumur 8 – 12 minggu, dengan berat badan 20 – 35 gram, dibagi menjadi empat perlakuan dengan enam kali ulangan, yaitu kelompok P0 dengan pemberian aquades steril, kelompok P1 dengan pemberian pegagan 125 mg/kg bb, kelompok P2 dengan pemberian pegagan 250 mg/kg bb, dan kelompok P3 dengan pemberian 500 mg/kg bb. Setelah 14 hari seluruh mencit diinfeksi dengan S. typhi. Pada hari ke 15 dilakukan nekropsi untuk pengambilan sampel berupa ginjal dan dibuat preparat histologi. Selanjutnya dilakukan pengamatan terhadap gambaran mikroskopis yang meliputi perubahan : infiltrasi sel radang, perdarahan, degenerasi vakuola dan nekrosis. Metode pewarnaan menggunakan Haematoxylin dan Eosin (HE). Hasil penelitian menunjukkan perlakuan P0 berbeda nyata dengan P1, P2, dan P3. Demikian juga dengan derajat kerusakan ginjal tersebut. Semakin tinggi dosis pegagan yang diberikan semakin berpengaruh terhadap perbaikan struktur mikroskopis ginjal.