I Nengah Suwastika
Tadulako University

Published : 48 Documents
Articles

Pertumbuhan Organ Tanaman Buah Naga(Hylocerus undatus) Pada Medium Ms Dengan Penambahan Bap Dan Sukrosa Sulistiami, Ari; Waeniati, Waeniati; Suwastika, I Nengah
Online Journal of Natural Science FMIPA Vol 1, No 1 (2012)
Publisher : Online Journal of Natural Science FMIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.2012/

Abstract

Dragon fruit (Hylocyrus undatus) is a commercial-cultivated plant that has a high economic value and believed to be a healthy-food. Propagation of seedling by tissue culture technique is challenging step during cultivation of these plant. This research was aimed to determine the efeect of sucrose and BAP concentration in the dragon fruit (H. undatus). This experiment was arranged in completely randomized  design with 4 treatments and 3 replications. The treatmnts were : MS0 + 0,5 ppm BAP + 35 g/L sucrose (A1), MS0 + 0,5 ppm BAP + 405 g/L sucrose (A2), MS0 + 0,5 ppm BAP + 45 g/L sucrose (A3), and MS0 + 0,5 ppm BAP + 50 g/L sucrose (A4). The results showed that all medium tested were suitable medium in inducing organs, including emerge of buds, thorns and roots. The best medium in inducing organogenesis of the plant were MS0 + 0,5 ppm BAP + 50 g/L sucrose.
Organogenesis Tanaman Jeruk Keprok (CITRUS NOBILIS LOUR.) Secara In Vitro Pada Media MS Dengan Penambahanberbagai Konsentrasi IAA (INDOLE ACETID ACID) Dan BAP (BENZYL AMINO PURIN) Harliana, Harliana; Weaniati, Weaniati; Muslimin, Muslimin; Suwastika, I Nengah
Online Journal of Natural Science FMIPA Vol 1, No 1 (2012)
Publisher : Online Journal of Natural Science FMIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.2012/

Abstract

Research on"Organogenesis of Tangerine orange (Citrus nobilisLour) onMSmediumsupplemented withvariousconcentrations ofIAAandBAP," has been performedatthe Tissue CultureLaboratory ofthe Faculty ofForestry, Tadulako University, Palu during the period of January andMay 2012. The goal of this study was to determine the optimum growth factor combination of IAA and BAP in MS based medium, for organogenesis of the plant. This research was arranged on Completely RandomizedDesign(CRD) withsix treatments and three replications. Growth factor combinations tested in this study were:  0.1ppm IAA + 0.4ppmBAP(C1), 0.1ppm IAA + 0.6ppm BAP(C2), 0.1ppmIAA+0.8ppmBAP(C3), 0.1ppm IAA + 1.0ppmBAP(C4), 1.0ppm IAA + 0.6ppmBAP(C5), and1.0ppmIAA+1.0ppmBAP(C6). The result indicating that the best organogenesis of Orange explant was in MS medium supplemented with 0.1 ppm IAA and 1.0 ppm BAP (C4). This medium was suitable in inducing shoot and leaf, faster than its in other medium. Explant on this medium was also produced higher number of leaves than it on other treatments.
Perbanyakan Tanaman Melon (Cucumis melo L.) Secara In Vitro Pada Medium Ms Dengan Penambahan Indole Acetic Acid (IAA) Dan Benzil Amino Purin (BAP) Lidyawati, Ni Nyoman; Waeniati, Waeniati; Muslimin, Muslimin; Suwastika, I Nengah
Online Journal of Natural Science FMIPA Vol 1, No 1 (2012)
Publisher : Online Journal of Natural Science FMIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.2012/

Abstract

The research on organogenesis of melon (Cucumis melo L.) was done at Tissue Culture Laboratory Forestry Faculty UNTAD Palu, during January until May 2012. The aim of this research was to determine the best concentration of IAA and BAP hormones in inducing organs of this plant.  Sterile seedling of melon was used as explants in this work. The experiment was arranged in  Completely Randomized Design (CRD)  with  4 treatments and 4 replications. The treatments were MS0 + 0,1 ppm IAA + 0,5 ppm BAP (M1), MS0 + 0,1 ppm IAA + 0,7 ppm BAP (M2), MS0 + 0,1 ppm IAA + 0,9 ppm BAP (M3) and MS0 + 0,1 ppm IAA + 1 ppm BAP (M4). Parameters observed on this study were the day appear of shoot and leaves, the number of shoots and leaves and also the present of callus and root. The result showed that the best medium for organs induction was MS0 + 0,1 ppm IAA + 1 ppm BAP, which shoot and leaf emerged in the day of 3,75 and 5,75 after induction, respectively. This media was also induced the number of shoots and leaves, i.e 4,75 and 8,75, respectively. Nevertheles this media was not suitable enough in inducing root formation.
Pengaruh Penambahan Air Kelapa Dan Berbagai Konsentrasi Hormon 2,4-D Pada Medium Ms Dalam Menginduksi Kalus Tanaman Anggur Hijau (Vitis vinifera L.) Dwi PYD, Niluh Made; Waeniati, Waeniati; Muslimin, Muslimin; Suwastika, I Nengah
Online Journal of Natural Science FMIPA Vol 1, No 1 (2012)
Publisher : Online Journal of Natural Science FMIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.2012/

Abstract

Green grape (Vitis vinifera L.) is popular cultivar of grape that produce valuable of table fruit whic usually grown via vegetative propagation. Another  chalanging tecnology in grape propagate is by induction of callus in tissue culture tecnique. The study on grape callus induction was done at Tissue Culture Laboratory-Forestry Faculty-Tadulako University Palu, during periode of January 2012 until May 2012. This experiment was arranged in completely randomized design with 3 different mediums as treatments and 3 replications. The treatments were MS0 + 2,4-D 0.5 ppm + 10% coconut water (D1), MS0 + 2,4-D 1 ppm + 10% coconut water (D2), dan MS0 + 2,4-D 1.5 ppm + 10% coconut water (D3). The observed parameters in this research were the days appear of callus, percentage of explant producing callus (%), also observation on morphology and the cell of callus. The result showed that the MS medium with 10% coconut water and 2.4-D could induce the callus of the green grape and the best medium was MS0 + 2.4-D 1.5 ppm + 10% coconut water (D3). It was shown by responding in emerging of callus in 11 days after induction, the percentage of explant producing callus was 76.67%. Produced callus was active in cell proliferation,  and it has a compac texture, with brown-greeny color of callii.
ORGANOGENESIS TANAMAN ANGGUR HIJAU (Vitis vinifera L.) PADA MEDIUM MSDENGAN PENAMBAHAN IAA (Indole Acetid Acid) DANBERBAGAI KONSENTRASI BAP (Benzil Amino Purin) Tajuddin, Riska; Suwastika, I Nengah; Muslimin, muslimin
Online Journal of Natural Science FMIPA Vol 1, No 1 (2012)
Publisher : Online Journal of Natural Science FMIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.2012/

Abstract

Research onorganogenesisof Grape (Vitis viniferaL.) on MS medium supplemented with IAA and various sconcentrations of BAP, was conducted at the Tissue Culture Laboratory, Faculty of Forestry Untad Palu, during periode of Januari and April 2012.This research was aimed to obtain the best combination ofIAA and BA Pin MS medium that stimulate organogenesis of Grape. This experiment was arranged in completely randomized design with 4 treatments and 3 replications.The treatmentswere: MS medium+ 0.1ppm IAA+ 0.1 ppm BAP(B1), MS medium+ 0.1 ppm IAA +0.3 ppm BAP (B2), MS medium + 0.1ppm IAA + 0.5ppm BAP(B3) and MS medium+ 0.1 ppm IAA+0.7BAP(B4). Parameters observed in this study were the days appear of shoots, the days appear of leaves, percentage of explant producing shoots (%), and the presence or absence of roots. The results showedthat MS medium+ 0.1 ppm IAA+0.7ppm BAP(B4) gived the best results in speedupof shootsandleaves,in 7 days after induction.
Induksi Kalus Tanaman Kakao (Theobroma cacao L.) Pada Media MS Dengan Penambahan 2,4-D, BAP Dan Air Kelapa Ariati, Sri Niken; Waeniati, Waeniati; Muslimin, Muslimin; Suwastika, I Nengah
Online Journal of Natural Science FMIPA Vol 1, No 1 (2012)
Publisher : Online Journal of Natural Science FMIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.2012/

Abstract

Study on callus induction of cacao (Theobroma cacao L.) was conducted at the Tissue Culture laboratory, Forestry faculty, Tadulako University, Palu, during periode of January until April 2012. This research was aimed to obtain the best medium formulation for induction and growth of cacao callus. Calli was induced from embryo  on MS based medium containing of 2,4-D, BAP and coconut water. The intact plant as explant was taken from Local Farming. This experiment was arranged in completely randomized  design with 4 treatments and 3 replications. The treatments were : MS medium + 2 ppm 2,4-D + 15% coconut water (N1), MS medium + 2 ppm 2,4-D + 0,2 ppm BAP, 15% coconut water (N2), MS medium + 2 ppm 2,4-D + 0,2 ppm BAP (N3), and MS medium + 2 ppm 2,4-D (N4). Parameters observed in this study were the days appear of callus, percentage of explant producing callus (%), also observation on morphology and the cell of callus. The best medium for callus  induction was MS medium + 2 ppm 2,4-D + 15% coconut water (N1). This medium produces healthy-compac callus which active in cell proliferation, in 6 days after induction.
Daftar Isi Natural Science: Journal of Science and Technology Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 8, No 2 (2019): Volume 8 Number 2 (August 2019)
Publisher : Univ. Tadulako

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Abstract

Daftar Isi Natural Science: Journal of Science and Technology
BACTERIAL MER GENES ARE POTENTIAL GENETIC MATERIAL FOR BIOREMEDIATION ON MERCURY POLUTION Suwastika, I Nengah
Online Journal of Natural Science FMIPA Vol 2, No 1 (2013): Volume 2 Number 1 (March 2013)
Publisher : Online Journal of Natural Science FMIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.2012/

Abstract

Remediation over mercury-contaminated areas become major concern and needs to be solved. Recently, various parts of ecosystem were contaminated because of increase of mercury emission into environment. Methyl-mercury is the most toxic form, which commonly found in contaminated area. This pollutant was rapidly increased due to uncontrolled emission of mercury from power plan, gold mining process and from other sources. The one possible and reasonable method in mercury bioremediation is by using genetically modified plant expressing bacterial-mercury resistance gene. Group of Mer gene was characterized encode mercury transporter and mercury detoxification protein in bacteria. On this study, Blast search on database based on known Mer protein sequence, it shows that these proteins were conserved among bacteria. These groups of genes are potentially to be cloned and transformed into plant for phytoremediation purposes. In order to enhance mercury uptake and reduce its toxicity, multiple genes transformation are considered to be effective approach for transgenic plant construction. This transgenic plant should survive in medium with high concentration of mercury and it should have activities in mercury uptake, detoxification, and Volatilization.
Induksi Kalus Klon Kakao (Theobroma cacao L) Sulawesi 2 Pada Medium MS Dengan Penambahan 2,4-D, BAP Dan Air Kelapa Urfiana, Urfiana; Haliana, Haliana; Muslimin, Muslimin; Suwastika, I Nengah
Online Journal of Natural Science FMIPA Vol 2, No 1 (2013): Volume 2 Number 1 (March 2013)
Publisher : Online Journal of Natural Science FMIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.2012/

Abstract

Sulawesi 2 cacao clone is one of the clones which grown by local farmer in Central Sulawesi. One problem in cacao development in this time is the availability of high quality of seeds. One posible way in overcoming of this problem is through the application of tissue culture techniques via embryogenesis. The early stages of embryogenesis is callus induction, which aim to get emryonic callus cells and it can be a model in cacao research. This study was conducted over March and June 2013 in the Tissue Culture Laboratory, Faculty of Forestry Tadulako University. Explant that used was Stamen of flower of Sulawesi 2 cacao clone. This experiment was based on a complete randomized design (CRD) with 4 treatments, and it were repeated 3 times. On every single unit was growth 10 of explants. Different medium as treatments tested were: MS0 + 2 ppm 2,4-D + (15%) Coconut water (MK1), MS0 + 2 ppm 2,4-D + 0.2 ppm BAP + (15%) Coconut water (MK2) , MS0 + 3 ppm 2,4-D + (15%) coconut water (MK3), ms0 + 3 ppm 2,4-D + 0.2 ppm BAP + (15%) coconut water (MK4). Callus development were observed based on the emergence of callus, the percentage of explants producing callus, and cell callus morphology. Callus induction was done under dark condition and  temperature of 26º C. The results showed that all of the tested treatments can induce the callus of cacao. The best medium was MS medium + 3 ppm 2,4-D + (15%) Coconut water (MK3), characterized by the appearance of white, callus in intermediate-type, the callus mass was relatively larger than its under others treatments. Under this treatment, explant can produce uniform-relatively big cell and active in proliferation in 10 days after culture
ORGANOGENESIS TANAMAN BAWANG MERAH (ALLIUM ASCALONICUM L.) LOKAL PALU SECARA IN VITRO PADA MEDIUM MS DENGAN PENAMBAHAN IAA DAN BAP Rufaida, Anna; Waeniaty, Waeniaty; Muslimin, Muslimin; Suwastika, I Nengah
Online Journal of Natural Science FMIPA Vol 2, No 2 (2013): Volume 2 Number 2 (August 2013)
Publisher : Online Journal of Natural Science FMIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.2012/

Abstract

Research on plant organogenesis Shallots (Allium ascalonicum L.) local Palu on MS medium with the addition of IAA and BAP, have been carried out in March and July 2013 in the Tissue Culture Laboratory, Faculty of Forestry Tadulako University. This study aims to get a combination of the concentration of IAA and BAP in MS medium onion plant organogenesis spur. Explants were used in the form of lateral shoots from the onion bulbs. This experiment is based on a complete randomized design (CRD) with 4 treatments, each treatment was repeated 5 times and every single unit test using a 2 explants. Growth media as treatments tested were: A1 (MS0 + 0.01 ppm IAA + 1 ppm BAP), A2 (MS0 + 0.05 ppm IAA + 1 ppm BAP), A3 (MS0 + 0.1 ppm IAA + 1 ppm BAP), A4 (MS0 + 0.5 ppm IAA + 1 ppm BAP). Judging from the appearance of the root, plantlet height, number of leaves, number of shoots, number of roots, chlorophyll content and the number of stomata per explant cultures tested The results showed that all treatments tested were able to induce organ onion crop Local Palu.. Based on these parameters, A1 is the best media in promoting organogenesis Local onion Palu. The media gives the best results appear to speed root, shoot number, leaf number and chlorophyll content. Other than that, the media A4 is also the best medium for the initiation stage plantlets before acclimatization. The media gives the best results for the number of stomata, while emerging roots and number of roots.