Bambang Sutrisno
Fakultas Kedokteran Hewan Universitas Gadjah Mada

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Lesi Patologik Organ dan Jaringan Ikan Nila (Oerochromis niloticus) yang Diinfeksi Bakteri Staphylococcus sp. Sutrisno, Bambang; Purwandari K., Yuli
Jurnal Sain Veteriner Vol 22, No 1 (2004)
Publisher : Fakultas Kedokteran Hewan

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Lesi Patologik Organ Dan Jaringan Ikan Nila (Oerochromis nilaticus) Yang Diinfeksi Bakteri Staphylococcus sp. Sutrisno, Bambang; Purwandari K., Yuli
Jurnal Sain Veteriner Vol 22, No 1 (2004)
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PENGARUH TEMPERATUR TERHADAP PATOGENESITAS OOSISTA Eimeriu tenella PADA AYAM PEDAGING Sri Rohayati, Ery; Rahmawati, Dewi; Sutrisno, Bambang
Jurnal Sain Veteriner Vol 1, No 29 (2011)
Publisher : Fakultas Kedokteran Hewan

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Koksidiosis adalah problem industri ayam yang pencegahan penyakit tersebut masih menjadi masalah di Indonesia. Penelitian ini bertujuan untuk mengetahui pengaruh temperar terhadap patogenesitas oosistaEimeria tenella pada ayam pedaging. Sebanyak 15 ekor day old chick (DOC) pedaging umur 17 hari dibagi menjadi tiga kelompok, masing-masing terdiri dari 5 ekor ayam. Setiap ayam dari kelompok A, B, dan C,masing-masing kelompok diinfeksi secara oral dengan 5000 oosista yang ditretmen dengan temperatu 4"C selama 17 hari, dengan 5000 oosista yang ditretmen pada temperatu 50"C selama 15 hari dan dengan 5000oosista yang ditretmen dengan temperatur ruang(27"C). Ayam dinekropsikan pada hari ke-5 dan ke-7 setelah infeksi untuk dilihat gambaran histopatologinya. Gambaran lesi sekum dianalisis dengan metode deskriptif, sedangkan derajat lesinya dianalisis dengan metode Rank test. Hasil penelitian menyimpulkan bahwa temperaturberpengaruh terhadap patogenesitas oosista E . tenella dan untuk mematikano osista E . tenella disarankan dengan pemanasan 50 "C selama 15 hari.Kata kunci: koksidiosis, Eimeria tenella, lesion score, gambaran histopatologi
STUDI PERKEMBANGAN DAN SITOPATOLOGI Eimeria tenella PADA MEMBRANCHORIOALLANTOIS Sutrisno, Bambang; Prastowo, Joko
Jurnal Sain Veteriner Vol 21, No 1 (2003)
Publisher : Fakultas Kedokteran Hewan

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Penelitian ini bertujuan untuk mengetahui perkembangan dari siklus hidup Eimeria tenella gambaran histopatologik membran chorioallontois sebagai akibat infeksi sporosista dan oosista Eimeria tenella, untuk mengetahui kemungkinan penggunaan membran chorioallantois sebagai medium k-ultur Eimeria tenella. Penelitian ini menggunakan 50 butir telur ayam berembrio umur 10 hari yang dibagi ke dalam 5 kelompok, masing-masing 10 butir. Kelompok 1, telur diinfeksi dengan 5.000 oosista, kelompok II diinfeksi dengan 10.000 oosista, kelompok 111 diinfeksi dengan 25.000 sporosista, kelompok IV diinfeksi dengan 50.000 sporosista dan kelompok V diperlukan sebagai kontrol. Telur-telur tersebut diinkubasi pada suhu 41°C selama 6 hari. Mulai hari ke 2 setelah infeksi, dua telur dad masing-masing kelompok diperiksa dengan membuka kerabangnya, membran chorioallantois diamati untuk melihat perubahan makroskopik dan histologik. Hasil yang didapat adalah kelompok I dan II tidak ada perbedaan dan oosista tidak berkembang bahkan mengalami nekrosis. Kelompok III dan /V menunjukkan perkembangan di dalam membran chorioallantois, hari ke 2 setelah infeksi sel epitel membesar dan terisi oleh sporozoit, hari ke 3 setelah infeksi sudah ada skizon generasi I, hari ke 4 setelah infeksi sel-sel epitel membesar dan ada stadium makrogamet, skizon generasi II dan zigot, dan had ke 5 setelah infeksi sudah terdapat stadium oosista. Inokulasi sporosista Eimeria tenella pada membran chorioallantois dapat berkembang menjadi stadium berikutnya, sedang inokulasi oosista Eimeria tenella pada membran chorioallantois tidak menunjukkan perkembangan. Membran chorioallantois dapat digunakan sebagai medium kultur Eimeria tenella.Kata kunci : Chorioallantois, Eimeria tenella, Skizon , Makrogamet
The Pathological Study of Suspected Swollen Head Syndrome in Broiler Chickens Sutrisno, Bambang; Widyarini, Sitarina; Wasito, R; Wuryastuty, Hastari
Jurnal Sain Veteriner Vol 31, No 1 (2013)
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The  objective of this study was to determine the pathological lesions of the broiler chickens having the clinical signs that appeared to be the swollen head syndrome (SHS),  such as simetrically and bilaterally swollen periorbital areas. Eighteen broiler chickens were collected from three poultry farms of the three diffrerent areas, e.g. Kulonprogo, Sleman and Bantul at Special Province of Yogyakarta, respectively. Six of those broiler chickens were selected from each poultry farm consisted of three SHS- broiler chickens and three non SHS-broiler chickens. All of broiler chickens were necropsied, observed anatomic patologic lesions, and then the organs of interest were processed and stained routinely with hematoxylin and eosin for histopathologic examination. Subcutaneus edema and cellulitis were found in the frontal and occipital skin. Based on the histopathological examination, purulent and granulomatous cellulitis and lymphocytic perivasculitis, and subcutaneus edema were found in the frontal and occipital skin. Other lesions, such as: necrotic, lymphocytic sinusitis, tracheitis and pneumonia accompanied by atrophy of mucosal glands were also present. It is concluded that the pathological lesions of SHS in the broiler chickens were found not only in the frontal and occipatal skin, but also in both lower and upper respiratory tracts.      
Comparation Protection Level of Newcastle Disease in Broiler Wibowo, Sarwo Edy; Asmara, Widya; Wibowo, Michael Haryadi; Sutrisno, Bambang
Jurnal Sain Veteriner Vol 31, No 1 (2013)
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Newcastle Disease (ND) is both  respiratory and digestive diseases in poultry caused by avian paramyxovirus type 1 (APMV-1). Field data showedthat there were still many cases of Newcastle Disease faced by farmers despite of vaccination programs had been  doneroutinely. The aim of this research is to find out the effectiveness of  some routine ND vaccination program in broiler chiken challengedeither with viscerotropic velogenic Newcastle Disease (VVND) virus or virulent ND virus from field isolates. One hundred broiler chickens were divided into 4 groups of 25 each. In theGroup I,vaccination was carried out at day 1 with combination of ND-IB live vaccineand ND killed vaccine, and booster at day 18 with live ND vaccine, in the Group II, chickens were vaccinated with live ND-IB vaccine at day 1 and day 18 and  in the Group III, chickens  were vaccinated with live ND-IB vaccine at day 1 and vaccinated with ND live vaccine at day 18. Challenge test performed in twenty broiler chickens of each group with virulent ND that has chicken lethal dose fifty (CLD50) 4,8. Virus preparation 26 and then diluted to 10-4, to obtain dilution 10000. Twenty chicken from each group were then given 0.5 cc dilution of 6 HA virulent virus at 28 days of old. Six challenged chicken from group I showed ND clinical symptom and were eventually death.  This mean that the vaccine program provided 70% protection. Whereas all challenged chicken from the Groups II and III were sick, then died meaning that these vaccination programs did not give any protection at all. Bsed on the present study, it is concluded that the administration of ND live vaccine priming along with ND killed vaccine is needed to improve the protection against velogenic NDV.    
Cytotoxic Tobacco Smoke in The Cornea of Wistar Rats Fed Turmeric (Curcuma Domestica Val.) Extract Kusumawardani, Agapietta; Sarwendah, Kurniati; Rahmad, Laelatul; Milah, Nida Ul; Herliyani, Novi; Sutrisno, Bambang; Wuryastuty, Hastari; Wasito, R
Jurnal Sain Veteriner Vol 31, No 1 (2013)
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Abstract An increasing number of smokers who tend to rise rapidly from year to year has become a major focus of public health issues. Exposure to environmental tobacco smoke, containing toxic chemicals can trigger acute or chronic inflammation and oxidative stress in the eyes. To overcome this condition, phytotherapeutic and/or phytopreventive approaches which are efficatious, safe and cheap is needed. One of spices from Indonesia which is greatly possible to be promoted as a herbal medicine, is a phytochemical curcumin in turmeric. This present study was conducted to identify and determine the effects of turmeric extract (Curcuma domestica Val.) as phytotherapeutic and/or phytopreventive in the eyes lesion of Wistar rats induced by cigarette smokes. Twenty male Wistar rats at 3 months old were divided randomly into four groups of five each. Those were the control group (KI), the group given 9 cigarettes smoke/day (K II), the group given turmeric extract 1 ml 0, 25% /day (K III) and the group given 9 cigarettes smoke/day and 0, 25% turmeric extract 1 ml /day (K IV) for 60 days. Water and feed was given ad libitum. At the end of the experiment, all rats were euthanized with ether, necropsied andeyes balls were collected, fixed formalin 10% and stained with routine hematoxylin-eosin. The results of the present study indicated that 9 cigarettes smoke/day increased mitotic activity and caused hyperplasia in the corneal epithelial cells (K II), and addition of turmeric extract 0, 25% 1 ml per oral for 60 days prevented both mitotic activity and hyperplasia in the corneal epithelial cells (K IV). Extract of turmeric (Curcuma domestica Val.) has been proved to have herbal therapeutic and/or preventive effects againts the pathotoxicological cigarette smokes. Further study needs to be done to more evaluate pathogenesis of the eyes lesions and phytotherapeutic and/or phytopreventive effects of turmeric extract as well.   
The Effects of Turmeric Extract (Curcuma domestica Val) on Cigarette Smoke Cardiotoxicity in The Wistar Rats Millah, Nida Ul; Kusumawardani, Agapietta; Rahmad, Laelatul; Herliyani, Novi; Sarwendah, Kurniati; Sutrisno, Bambang; Wuryastuty, Hastari; Wasito, R
Jurnal Sain Veteriner Vol 31, No 2 (2013)
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Abstract            The objective of this research is to determine the effects of turmeric (Curcuma domestica Val) extract administration toward heart histopathologic lesions of male albino Wistar rats + 3 months old exposed by cigarette smoke. Twenty male Wistar rats were divided randomly into four groups of five each: Group I was given neither cigarette smoke exposure nor turmeric extract administration (control) (KI), Group II was given cigarette smoke exposure of 9 cigarettes/ day (KII), Group III was given 1 ml turmeric extract 0.25% (KIII) and Group IV was given cigarette smoke exposure of 9 cigarettes/ day and 1 ml turmeric extract 0.25% (KIV). After 2 months of treatment, necropsy was conducted to all rats and the heart samples were collected to be processed histopatologically and stained routinely with hematoxylin-eosin. The results of the present study indicated that the cigarette smoke caused cardiomyopathy in KII marked by the histopathologic lesions such as vacuolation, homogeneous eosinophilic cytoplasms, edema and necrosis of myocardia, also congestion and mild hemorrhages (KII). Whereas, rats in Groups KI, KIII and KIV had normal histologic structures of myocardia. It is concluded, that turmeric extract could be a preventive herbs toward cardiotoxic effects of cigarette smoke. Further research has to be conducted to determine the anti cardiomyopathy mechanism of turmeric rhizome due to cigarette smoke exposure.
KERANCUAN YURIDIS KEWENANGAN PERLINDUNGAN DAN PENGELOLAAN LINGKUNGAN HIDUP DALAM PERSPEKTIF OTONOMI DAERAH Sutrisno, Bambang
DIH Vol 9, No 17 (2013)
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Terbitnya Undang-undang Nomor 32 Tahun 2004 Tentang Pemerintahan Daerah, merupakan konsekuensi konstitusional dari amandemen UUD 1945, khususnya pasal 18 ayat (5) yang menetapkan: “Pemerintah daerah menjalankan otonomi seluas-luasnya, kecuali urusan pemerintahan yang oleh Undang-undang ditentukan sebagai urusan pemerintah pusat”. Amanat UUD 1945 diatas, ditindaklanjuti dengan menuangkannya dalam Undang-undang Nomor  32 Tahun 2004 tentang Pemerintahan Daerah, sebagaimana yang telah diubah dengan Undang-undang Nomor 12 Tahun 2008 tentang Perubahan Kedua Atas Undang-undang Nomor 32 Tahun 2004,  khususnya pasal 10, 13, dan pasal 14. Ketentuan Pasal 10 ayat (3) menetapkan rincian urusan pemerintahan yang menjadi wilayah kewenangan pemerintah pusat, sementara ketentuan pasal 13 dan 14 menetapkan rincian urusan pemerintahan yang menjadi hak, kewajiban dan tanggung jawab pemerintah daerah  propinsi, kabupaten dan kota, yang salah satunya adalah tentang pengendalian lingkungan hidup. Berpedoman pada ketentuan di atas, maka tanggung jawab pengendalian lingkungan hidup menjadi salah satu urusan wajib pemerintah daerah. Sedangkan menurut Undang-undang Nomor 23 Tahun 1997 tentang Pengelolaan Lingkungan Hidup, tanggung jawab pengelolaan dan pengendalian lingkungan hidup, sepenuhnya menjadi wewenang Pemerintah Pusat.Key word : pengelolaan lingkungan hidup, otonomi daerah
Isolation and Identification of Egg Drop Syndrome Virus with Hemagglutination and Hemagglutination Tests Fitrawati, Fidyah; Wibowo, Michael Haryadi; Amanu, Surya; Sutrisno, Bambang
Jurnal Sain Veteriner Vol 33, No 1 (2015)
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Egg drop syndrome (EDS) is a disease that attacks layer hens in the production phase causing failure of peak eggs production, decreased in eggs production, and presence of eggs without shell. This study was conducted to isolate and identify the EDS virus in the chicken layer that was diagnosed as a disease of EDS by hemagglutination (HA) and  hemagglutination inhibition (HI) assays. Specific pathogen free (SPF) layerchickens which were passing through the production phase fed with food which was mixed with egg without shell from SR/WNO/2011. The chicken together with chicken FF/Sleman/2011 were dissected when pathological lesions, such as the dents or palor eggs observed. The uterine tissues were then collected for samples. Infundibulum of chicken FF/Sleman/2011 was explored and was found out that the eggs were lack ofegg shells. The eggs were then washed using sterile PBS. The three subsequent samples were propagated in the allantoic fluid of embryonated duck eggs for 16 days. Allantoic fluid was harvested after being incubated for 4 days. It was then tested by HA and HI assay by use of avian influenza virus (AIV), Newcastle disease virus (NDV), and EDS anti serum. The HA and HI test with EDS anti serum used chickens erythrocytes in percentageof 0,8. The HA test in uterine sample of both SR/WNO/2011 and FF/Sleman/2011 showed the titer 23 HA units and egg washed water sample of FF/Sleman/2011 showed titer 22 HA units. The HI test for comparison with ND and AI anti serum was negative, while the test with EDS anti serum showed positive results. Based on the HA and HI test results, it was concluded that the virus grown in the allantoic fluid is EDS virus.