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Insidensi Nematoda Gastrointestinal dan Protozoa pada Monyet Ekor Panjang (Macaca fascicularis) Liar di Taman Wisata Alam (TWA) Pulau Weh Sabang Rahmi, Erdiansyah; Hanafiah, Muhammad; Sutriana, Amalia; Hambal, Muhammad; Wajidi, Farid
Jurnal Ilmu-Ilmu Peternakan JIIP Volume XIII No. 6 Mei 2010
Publisher : Jurnal Ilmu-Ilmu Peternakan

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The research aims to find out the  incidence of gastrointestinal parasite in wild long tail macaque in wildlife  reserve  park, Weh  Island, Sabang. For  that purpose,  fecal  specimens  from 25 wild  long  tail macaques  in Weh  Island-  Sabang were  collected.  The  fresh  faeces  samples were  then  preserved  using AFA  (alcohol-formal-acetic)  liquid  prior  to  be  examined  in  Parasitology  Laboratorium,  Faculty  of Veterinary Medicine, Syiah Kuala University.   The  presence of gastrointestinal  parasite was  observed using centrifuge method as well as Boray  sedimentation method. The obtained data were tabulated and analyzed  descriptively.  The  result  revealed  that  two  parasite  species  were  found  in  gastrointestine; Eimeria  spp.    (12%)  and  Strongloides  spp.  (8%).  It  could  be  concluded  that  the  prevalence  of gastrointestinal  parasite  in wild  long  tail macaque  in wildlife  reserve  park, Weh  Island-  Sabang was considered mild.
Aktivitas Hepatoprotektif Ekstrak Etanol Daun Kucing-kucingan Acalypha indica L.) pada Tikus Putih (Rattus Novergicus) yang Diinduksi Parasetamol Armansyah TR, T; Sutriana, Amalia; Aliza, Dwinna; Vanda, Henni; rahmi, erdiansyah
Jurnal Ilmu-Ilmu Peternakan JIIP Volume XIII No. 6 Mei 2010
Publisher : Jurnal Ilmu-Ilmu Peternakan

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The  research  has  been  conducted  to  determine  hepatoprotective  effect  of  ethanolic  extract  of Acalypha  indica  L.  leaves  on  rats  (Rattus  novergicus)  induced  with  paracetamol.  The  study  was performed  following  a  completely  randomized  design, using  45 male  rats  (Rattus  novergicus)  divided into  5  groups.  Rats  in  group  1  (K1)  were  given  CMC  1  %  for  7  days  and  followed  by  aquadest administration 8 hours post given CMC on day 7. Group 2 was given CMC 1 % for 7 days and treated with paracetamol at dose 2,5 g/kg BW 8 hours post given CMC on day 7. Groups 3 (K3), 4 (K4), and 5 (K5) were pretreated with ethanolic extract of Acalypha indica L.  leaves at the dose of 50, 100, and 200 mg/kg BW for 7 days respectively, followed by paracetamol administration at the dose of 2.5 g/kg BW in the  next  8  hours  on  day  7. Paracetamol  hepatotoxicity  and  extracts  activity were measured  based  on SGPT and SGOT activity level on day 0, 8 hours after  last administration of  extract on day 7, and 24 hours post paracetamol administration. Data were analyzed using one way ANOVA. The result showed that  the  administration  of  ethanolic  extract  of Acalypha  indica  leaves  at  the  dose  of  50,  100,  and  200 mg/kg BW was  significantly  reduced SGPT  and SGOT  level  (P<0,05)  in  rats  that were  induced with paracetamol. Based on the results of SGPT and SGOT serum activity, it can be concluded that ethanolic extract of Acalypha indica L. potential as hepatoprotective agent.
Uji Tuberculin pada Kulit Monyet Ekor Panjang (Macaca fascicularis): dalam Upaya Pencegahan Penyakit Zoonosis Tuberkulosis (TBC) di Kawasan Wisata Pulau Weh Sabang Rahmi, Erdiansyah; Sutriana, Amalia; Aliza, Dwina
Jurnal Primatologi Indonesia Vol 7, No 1 (2010)
Publisher : Pusat Studi Satwa Primata LPPM-IPB

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Abstrak: Penelitian ini bertujuan untuk memperoleh informasi tentang penularan penyakit tuberkulosis (TBC)  pada monyet ekor panjang (Macaca fascicularis) di kawasan wisata Pulau Weh, Sabang dan untuk mendukung program pemerintah dalam menghasilkan Indonesia bebas TBC. Penelitian ini dilakukan dalam beberapa tahap setelah memperoleh persetujuan dan izin dari Balai Besar Konservasi dan Sumberdaya Alam Aceh dan instansi terkait di Sabang. Tahapan berikutnya menentukan lokasi, populasi, dan identifikasi monyet ekor panjang serta menentukan jumlah monyet ekor panjang per kelompok. Pemeriksaan hewan dengan melakukan sedasi menggunakan ketamin (10mg/kg) dan xylazine (2 mg/kg) secara intramuskuler. Skrining menggunakan tes tuberkulin metode (mammalian old tuberculin) dilakukan terhadap semua hewan 0,1 mL (135000 Tuberkulin Unit) secara intradermal pada palpebra. Hasil uji tuberculin dibaca pada 24, 48, dan 72 jam pasca suntik. Pengujian diulang pada minggu kedua. Data ditabulasi dan dianalisis secara deskriptif. Dari identifikasi populasi monyet di dua wilayah itu ditemukan satu kelompok monyet pada kilometer nol (N 05 ° 54 ´19,1 "E 95 ° 12´ 57,4") dengan populasi 14 ekor, sedangkan di wilayah Cot Murong ( N 05 ° 54 ´19,1 "E 95 ° 12´ 57,4") ditemukan dua kelompok dengan populasi 12 dan 14 ekor. Skrining tuberkulosis menggunakan metode ini menunjukkan bahwa tidak ada insiden TBC pada monyet ekor panjang di kawasan wisata Sabang.Abstract: This research was purposed to obtain the information about the evidence of Tuberculosis (TB) infectious disease on long tail macaque (Macaca fascicularis) in tourism area Weh Island, Sabang and to support the government’s program in generating Indonesia free TB, as well. This research was conducted on many stages, those were permits arrangement with BKSDA Aceh and Forestry Officer Sabang, location identification of long tail macaque populations, and identification of the amount of long tail macaque population in each groups. The monkeys chosen were restrained and anaesthetized using Ketamin (10mg/kg) and xylazine (2mg/kg) intramuscularly. Screening test using tuberculin skin test (TST) method (old mammalian tuberculin kit) was carried out to all samples, 0.1 mL (135000 Tuberculin Unit) on palpebrae intradermally. The effect of TST was examined at 24, 48, and 72 hours post treatment. The test was repeated on the second week. The data were tabulated and analyzed descriptively. From the identification of macaca populations in two areas was found one group of  macaca  in Kilometer Nol (N 05° 54’ 19,1" E 95° 12’ 57,4")  with the population of 14 macacas whereas in Cot Murong area (N 05° 54’ 19,1" E 95° 12’ 57,4") found two groups with the population of 12 and 14 macacas. TB screening test using TST method showed that there were no incidents of TB in long tail macaque in Sabang tourism area.Keywords : Tuberculin Skin Test, Sabang, Macaca fascicularis  
In vitro antimalarial activity of Jaloh leaves extract on Plasmodium falciparum Asmilia, Nuzul; Sutriana, Amalia; Rahmi, Erdiansyah; Sugito, .
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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Malaria still cause health problem in tropics including Indonesia, since its generate high mortality rate. Medication of Malaria has been performed long time ago without optimum result. One reason is the high rate of P.falciparum resistance on conventional antimalaria drug. This research aim to evaluate the activity of jaloh leaves extract (Willow leaves) on the inhibition of P.falciparum in vitro.This was a laboratory experimental research that using Indonesian isolates of cloroquin resistance P.falciparum obtained from NAMRU-2, Jakarta. P.falciparum isolate that has been synchronized were divided in to 3 groups of treatment : Jaloh leaves ethanolic extract, jaloh leaves ethyl acetate extract, and jaloh leaves n-hexane extract. Each group were then alloted into 5 dosage levels: 6,25ug/ml; 12,50ug/ml; 25,00ug/ml; 50,00ug/ml; and 100ug/ml. The observation of parasitemia degree were done 48 hours post-incubation in which the thick blood smear were colored with Giemsa and then examined using bioculer microscope. Data were analyzed using one way ANOVA and Newman-Keuls test. The result showed that the percentage of P.falciparum growth inhibition were sinificantly different (P<0.05) among treatments wherein ethanol extract and ethyl acetate extract more inhibit P.falciparum growth than the hexane extract. The administration of different dosage also resulted in significance different (P<0.05) of P.falciparum growth inhibition. This research concludes that jaloh leaves extract could inhibit P.falciparum growth.
UJI DAYA HAMBAT EKSTRAK BIJI BUAH PALA (Myristica fragrans Houtt) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli (TEST OF INHUBITORY POWER OF NUTMEG SEEDS (Myristica fragrans Houtt) EXTRCT ON GROWTH OF BACTERIA Escherichia coli) Syarifah, Raysa; Fakhrurrazi, Fakhrurrazi; Harris, Abdul; Sutriana, Amalia; Erina, Erina; Winaruddin, Winaruddin
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 3 (2018): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v2i3.8203

Abstract

ABSTRAKPenelitian ini bertujuan untuk mengetahui daya hambat ekstrak biji buah pala (Myristicafragrans Houtt) terhadap pertumbuhan bakteri Escherichia coli. Biji pala yang digunakan dalam penelitian ini diperoleh dari pasar tradisional Darussalam, Banda Aceh. Proses ekstrasi biji buah pala menggunakan pelarut Etanol 96% dengan tingkat konsentrasi yang berbeda yaitu 1%, 10%,20%.  Menggunakan kertas cakram kosong (Blank disk) yang telah dicelupkan ke dalam ekstrak biji buah pala masing-masing pengulangan dan antibiotik ampisilin yang diletakkan pada media MHA. Pengujian daya hambat dilakukan dengan metode difusi Kirby-Bauer menggunakan kertas cakram pada media Mueller hinton agar (MHA) dengan 5 perlakuan dan 3 pengulangan. Media agar diinkubasi pada suhu 36-37ºC selama 24 jam. Hasil penelitian ini menunjukkan bahwa bahwa ektsrak  biji  buah  pala  memiliki  daya  hambat  yang  lemah  terhadap  pertumbuhan  bakteri Escherichia coli.  Ekstrak biji buah pala pada konsentrasi 1% memiliki daya hambat sebesar 9,73 mm, konsentrasi 10% sebesar 8,5 mm, konsentrasi 20% sebesar 8 mm dan kontrol positif sebesar20,83 mm.Kata kunci : antibakteri, ekstrak biji buah pala, Escherichia coli.. ABSTRACTThis study aims to determine the inhibitory power of nutmeg extract (Myristica fragransHoutt) on the growth of Escherichia coli bacteria. The nutmeg seed used in this study was obtained from the traditional market of Darussalam, Banda Aceh. Extraction process of nutmeg seed using96% ethanol solvent with different concentration level that is 1%, 10%, 20%. Using blank disc paper (Blank disk) which has been dipped into nutmeg extract of each repeating and ampicillin antibiotic placed on MHA media. Inhibitory testing was performed by the Kirby-Bauer diffusion method using paper discs on Mueller hinton agar medium (MHA) with 5 treatments and 3 repetitions. Media to be incubated at 36-37ºC for 24 hours. The results of this study indicate that the nutmeg nut extraction has a weak inhibitory effect on the growth of Escherichia coli bacteria. The extract  of nutmeg  seed  at concentration  of 1%  has inhibitory power  of 9.73  mm,  10%concentration of 8.5 mm, 20% concentration of 8 mm and positive control of 20.83 mm.Keywords : antibacterial, nutmeg ekstact, Escherichia coli.
UJI ANTIBAKTERI EKSTRAK DAUN KELOR (Moringa oleifera L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus (Antibacterial Activity Test of Moringa oleifera L. Extracts on Staphylococcus aureus) Savitri, Elza; Fakhrurrazi, Fakhrurrazi; Fakhrurrazi, Fakhrurrazi; Harris, Abdul; Harris, Abdul; Erina, Erina; Erina, Erina; Sutriana, Amalia; Sutriana, Amalia; Lubis, Triva Murtina; Lubis, Triva Murtina
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 3 (2018): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v2i3.8227

Abstract

Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak daun kelor (Moringa oleifera L.) terhadap pertumbuhan bakteri Staphylococcus  aureus. Ekstrak daun kelor diekstraksi dengan cara maserasi yaitu menggunakan pelarut etanol  96%. Pengujian aktivitas antibakteri dilakukan dengan metode difusi agar  menggunakan kertas cakram yang  telah direndam dalam ekstrak etanol daun kelor dengan konsentrasi 20%, 40%, 60% dan 80% pada media Mueller Hinton Agar (MHA). Parameter yang diukur adalah besarnya diameter zona hambat yang  terbentuk disekitar kertas cakram. Hasil uji aktivitas antibakteri dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa ekstrak etanol  daun kelor memiliki daya hambat terhadap bakteri Staphylococcus  aureus yaitu pada konsentrasi 80% sebesar 14,02 mm (kategori kuat), 60% sebesar 12,03 mm (kategori kuat), 40% sebesar 9,00 mm  (kategori sedang), 20% sebesar 7,98 mm (kategori sedang), kontrol positif 28,63 mm  (kategori sangat kuat) dan kontrol negatif tidak menunjukkan efek antibakteri. Dapat disimpulkan bahwa ekstrak daun kelor memiliki antibakteri terhadap pertumbuhan bakteri Staphylococcus aureus.                The aim of this research is to observe the antibacterial activity of Moringa oleifera L. extract on Staphylococcus aureus. Moringa oleifera extract was extracted by maceration using ethanol 96%. The antibacterial activity test was carried out using diffusion method used paper disc which has been soaked in etanol extract of Moringa oleifera with concentration of 20%, 40% and 60% on Mueller Hinton Agar (MHA). Parameters measured were the diameter of inhibition zone formed around the paper disc. The results of antibacterial activity test were analyzed descriptively. The result showed that etanol extract of Moringa oleifera had inhibitory on Staphylococcus aureus. Moringa oleifera extract had inhibitory on Staphylococcus aureus at concentration 80% was 14,02 mm (strong category), 60% was 12,03 mm (strong category), 40% was 9,00 mm (middle category), 20% was 7,98 mm (middle category), positive control was 28,63 mm (very strong category) and negative control did not show antibacterial effect. It can be concluded that Moringa oleifera L. extract has an antibacterial effect on Staphylococcus aureus.
UJI ANTIBAKTERI EKSTRAK DAUN KELOR (Moringa oleifera L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus (Antibacterial Activity Test of Moringa oleifera L. Extracts on Staphylococcus aureus) Savitri, Elza; Fakhrurrazi, Fakhrurrazi; Fakhrurrazi, Fakhrurrazi; Harris, Abdul; Harris, Abdul; Erina, Erina; Erina, Erina; Sutriana, Amalia; Sutriana, Amalia; Lubis, Triva Murtina; Lubis, Triva Murtina
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 3 (2018): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v2i3.8227

Abstract

Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak daun kelor (Moringa oleifera L.) terhadap pertumbuhan bakteri Staphylococcus  aureus. Ekstrak daun kelor diekstraksi dengan cara maserasi yaitu menggunakan pelarut etanol  96%. Pengujian aktivitas antibakteri dilakukan dengan metode difusi agar  menggunakan kertas cakram yang  telah direndam dalam ekstrak etanol daun kelor dengan konsentrasi 20%, 40%, 60% dan 80% pada media Mueller Hinton Agar (MHA). Parameter yang diukur adalah besarnya diameter zona hambat yang  terbentuk disekitar kertas cakram. Hasil uji aktivitas antibakteri dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa ekstrak etanol  daun kelor memiliki daya hambat terhadap bakteri Staphylococcus  aureus yaitu pada konsentrasi 80% sebesar 14,02 mm (kategori kuat), 60% sebesar 12,03 mm (kategori kuat), 40% sebesar 9,00 mm  (kategori sedang), 20% sebesar 7,98 mm (kategori sedang), kontrol positif 28,63 mm  (kategori sangat kuat) dan kontrol negatif tidak menunjukkan efek antibakteri. Dapat disimpulkan bahwa ekstrak daun kelor memiliki antibakteri terhadap pertumbuhan bakteri Staphylococcus aureus.                The aim of this research is to observe the antibacterial activity of Moringa oleifera L. extract on Staphylococcus aureus. Moringa oleifera extract was extracted by maceration using ethanol 96%. The antibacterial activity test was carried out using diffusion method used paper disc which has been soaked in etanol extract of Moringa oleifera with concentration of 20%, 40% and 60% on Mueller Hinton Agar (MHA). Parameters measured were the diameter of inhibition zone formed around the paper disc. The results of antibacterial activity test were analyzed descriptively. The result showed that etanol extract of Moringa oleifera had inhibitory on Staphylococcus aureus. Moringa oleifera extract had inhibitory on Staphylococcus aureus at concentration 80% was 14,02 mm (strong category), 60% was 12,03 mm (strong category), 40% was 9,00 mm (middle category), 20% was 7,98 mm (middle category), positive control was 28,63 mm (very strong category) and negative control did not show antibacterial effect. It can be concluded that Moringa oleifera L. extract has an antibacterial effect on Staphylococcus aureus.
Characterization of Inhibin from Culture and Non Culture of Granulose Cells for Monoclonal Antibody of Inhibin Production Amiruddin, Amiruddin; Nizwan Siregar, Tongku; Sutriana, Amalia; Aliza, Dwinna; Armansyah, T.
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i1.9789

Abstract

This study has long-term objectives to obtain immunogenic prototype that can be used to induce multiple ovulation in goats. Working steps of this study were begun with the collection of ovarium from goats, collection of granulose cells, culture of granulose and characterization of molecular weight and isoelectric point (pI) of inhibin protein of granulose cells obtained from culture and non-culture of granulose cells, and followed by preparation of monoclonal antibody toward inhibin. The results showed that inhibin isolated either from culture or non-culture of granulose cells produced a 32 kDa band. Molecular weight of inhibin was measured by Western Blot. The 32 kDa band of SDS PAGE product appeared on Western Blot result was inhibin molecules produced by granulose cells collected fom culture and non-culture of granulose cells that can be identified by Mab-inhibin. Product of IEF gel electrophoresis suggested that inhibin molecule collected from culture of granulose cells has no charge at isoelectric points ranging from 5-6, depends on its total amino acid composition.
UJI TOKSISITAS AKUT EKSTRAK ETANOL DAUN MALAKA (Phyllantus emblica) TERHADAP MENCIT (Mus musculus). (Acute Toxicity Test of Ethanolic Extract of Malaka (Phyllantus emblica) Leaves on Mice (Mus musculus)) TR, T. Armansyah; Indriany, Sudi; Sutriana, Amalia; Rosmaidar, Rosmaidar; Asmilia, Nuzul; Panjaitan, Budianto; Aliza, Dwinna; Hamdan, Hamdan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5137

Abstract

ABSTRACT The aim of this research was to asses the acute toxicity of ethanolic extract of malaka leaves using lethal dose 50 (LD50) on mice (Mus musculus). Twenty male mice weighing between 20-30 g were randomly divided into 4 groups (group K1-K4) of 5 mice each. All mice in group K1, K2, K3, and K4 were administered ethanolic extract of malaka leaves with the dose of 2, 4, 8, and 16 g/kg bw, respectively. Single dose of ethanolic extract of malaka leaves were given by oral gavage prior to clinical observation . The observation period was 14 days post administration, for sign of toxicity symptom, weight loss, and mortality. The result showed that no mortality was observed in the experimental animals during this study. Slight reduction of body weight was observed in group K2, K3, and K4, and no toxicity sign was found during fourteen days of observation. The LD50 of ethanolic extract of malaka leaves was higher than 16 g/kg body weight, thus, the substance was practically non toxic substance.
Uji Preklinis fraksi etil asetat daun jaloh (Salix tetrasperma Roxb) sebagai antiplasmadium secara in vitro Asmilia, Nuzul; Sugito, Sugito; Sutriana, Amalia
Jurnal Sain Veteriner Vol 29, No 2 (2011): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.39532

Abstract

The aim of the present study was to find out the in vitro activity of ethyl acetate fraction of willow leaves (Salix tetrasperma Roxb) on Plasmodium folcifarum (P. falciparum) growth.