Putu Sutisna
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Experimental Infection of Taenia saginata eggs in Bali Cattle: Distribution and Density of Cysticercus bovis Sadra Dharmawan, Nyoman; Damriyasa, I Made; Kapti, I Nengah; Sutisna, Putu; Okamoto, Munehiro; Ito, Akira
Jurnal Veteriner Vol 10, No 4 (2009)
Publisher : Jurnal Veteriner

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Abstract

The objective of this study was to observe the development, distribution, and infection density ofTaenia saginata metacestodes in Bali cattle. Three Bali cattle were experimentally infected with T. saginataeggs which were collected from taeniasis patients. The experimental animal was inoculated with : i)1000,00 T. saginata; ii) 500,000 eggs; and iii) 1,000,000 eggs, respectivelly 100,000 (cattle 1), 500,000(cattle 2), and 1,000,000 (cattle 3) T. saginata eggs, respectively. To observe the development of cysticerci,all cattle were slaughtered at 24 weeks post infection. To observe their distribution and density, slicingwas done to the cattle?s tissues. The study results showed that cysts were found distributed to all muscletissues and some visceral organs such as heart, diaphragm, lungs, and kidney of the cattle infected with100,000 and 500,000 T. saginata eggs. Density of the cyst was in the range of 11 to 95 cysts per 100 gramsof tissue. The highest density was noted in the heart (58/100 grams) and in diaphragm (55/100 grams).This study has confirmed that T. saginata eggs derived from taeniasis patient in Bali, if infected to Balicattle can develop and spread to all muscle tissues and some visceral organs. From this study it wasconcluded that it is necessary to include the heart in the meat inspection at slaughter house for possibilityof T. saginata cyst infection.$?
PREVALENSI TAENIOSIS DAN SISTISERKOSIS DI BANJAR PAMESAN, DESA KETEWEL, GIANYAR, BALI Sutisna, Putu; Kaptil, I Nengah; Allan, James C.; Rodrigues-Canul, Rossanna
Majalah Kedokteran Udayana Vol 31, No 110 (2000): Majalah Kedokteran Udayana
Publisher : Majalah Kedokteran Udayana

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Abstract

Penelitian ini dikerjakan di Br Pamesan, Desa Ketewel, Gianyar (penduduk: 765 orang). Tujuan penelitian adalah untuk mengetahui angka prevalensi taeniosis dan sistiserkosis di banjar tersebut. Untuk menentukan prevalensi taeniosis dipergunakan teknik mikroskopi hapusan langsung dan koproantigen ELISA. Untuk menetapkan prevalensi sistiserkosis dipakai teknik imunoblot /Western blot. Selain itu juga dikerjakan wawancara memakai kuesioner terhadap subjek penelitian dan kepala rumah tangga, untuk mendapat data yang terkait dengan epidemiologi. Dari 156 feses yang diperiksa, ternyata 2 mengandung Taenia, jadi prevalensi taeniosis adalah 1,3%. Kedua kasus ternyata disebabkan oleh T. saginata. Dari 115 serum yang diperiksa, 6 menunjukkan hasil seropositip, berarti prevalensi sistiserkosis adalah 5,2%. Kasus tersebut terdiri dari 3 laki dan 3 perempuan, berumur antara 26-46 tahun. Di samping kelompok penelitian, 13 orang yang mengeluh mengeluarkan proglotida juga diperiksa, dan ternyata 8 diantaranya mengandung infeksi Taenia, terdiri dari 7 T. saginata dan 1 T. solium. Dua diantaranya menunjukkan hasil seropositip dengan pemeriksaan imunoblot, seorang terinfeksi T.solium dan yang lainnya T.saginata. Hasil penelitian ini lebih menegapkan lagi bahwa taeniosis dan sistiserkosis memang endemis pada penduduk Pulau Bali. Kata kunci: taeniosis/.sistiserkosis; mikroskopi; koproantigen; imunoblot; Bali ABSTRACTThis study was carried out in Banjar Pamesan, in the Village of Ketewel, District of Gianyar (population: 765). The study objective was to assess the prevalence of taeniosis and cysticercosis in the hamlets population. For determination of taeniosis prevalence the techniques used were direct smear microscopy and coproantigen ELISA. To determine the prevalence of cysticercosis, immunoblot/Western blot technique, using crude extract of T. soliuna cysticercus, was used. Interviews were also done to the study subjects and their respective heads of households to obtain a variety of data related to the epidemiology of taeniosis/ cysticercosis. Of 156 fecal samples examined. 2 were positive for Taenia, giving a prevalence of taeniosis of 1,3%. Both cases were caused by T. saginata. Of 115 sera examined, 6 showed presence of anti T. solium cysticercus antibody (prevalence of cysticercosis: 5,2%). The six cases consisted of 3 males and 3 females, aged between 26 and 46 years. Additional 13 persons reporting of having expelled proglottids were examined, and 8 were found to have taeniosis, 7 with T. saginata and I with T. soliuun. Two of them were found seropositive by immunoblot; who were infected with T.saginata and T solium respectively. This study has reconfirmed the endemicity of taeniosis and cysticercosis in the population of Bali. Key words: taeniosis/cysticercosis; microscopy; coproantigen; immunoblot; Bali
Present Situation and Problems of Cysticercosis in Animal in Bali and Papua Dharmawan, Nyoman Sadra; Swastika, Kadek; Putra, I Made; Wandra, Toni; Sutisna, Putu; Okamoto, Munehiro; Ito, Akira
Jurnal Veteriner Vol 13, No 2 (2012)
Publisher : Jurnal Veteriner

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Cysticercosis zoonotic parasitic disesase caused by Taenia solium larva, is a major public healthproblem. Cysticercosis results from the development of larval tapeworms in humans harboring adult T.solium or from ingesting soil containing eggs shed in the feces of humans, in areas where there are nolatrines. Humans are accidental intermediate hosts and pigs are the normal intermediate hosts. Clinicallythey are most serious when located in the central nervous system or in the eye where they persist formonths to years. Cysticercosis occurs worldwide primarily in developing country where pigs are raised,pork consumed and poor sanitation allows pigs’ access to human faeces. The occurance of the diseases isexpected to increase in relation to the growing demand for pork in those countries. In Indonesia cyticercosisis still a very important health problem, especially in Bali and Papua. The majority of the populations inBali and Papua are Hindus and Protestants/Catholics, respectively, so pork widely consumed in thoseislands. This brief review summarizes the present situation and problems of cysticercosis in animal inBali and Papua. Epidemiological data of the diseases were obtained from several sources.
The prevalence of Strongyloides stercoralis infection in rural population of Bali: A preliminary study Widjana, Dewa P.; Sutisna, Putu
Medical Journal of Indonesia Vol 10, No 3 (2001): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (462.697 KB) | DOI: 10.13181/mji.v10i3.28

Abstract

A cross-sectional study was carried out from March until September 1992 in 4 different geo-climatic rural villages in Bali to assess the prevalence of Strongyloides stercoralis infection related to age, gender and geo-climatic condition. The number of selected samples using multistage stratified random sampling based on age and gender was 2880. Strongyloides larvae in stool samples were identified by modified Harada Mori fecal culture technique. The results showed that the overall prevalence of Strongyloides infection was 1.6%; the prevalence in male was 1.2% and in female was 2.1%, however the gender difference was not statistically significant. In term of age, there was no statistically significant difference. The highest prevalence (2.4%) was found among 7-12 years children, followed by aged 7 years or less (1.6%), 18 years or over (1.6%), and 13-18 years (0.6%). The prevalence of Strongyloides infection related to geo-climatic conditions was significantly difference. The highest prevalence of Strongyloides infection was at wet highland (3.3%), followed by wet lowland (1.5%), dry highland (1.0%), and dry lowland (0.9%). In conclusion, the prevalence of Strongyloides stercoralis infection in Bali was very low. Prevalence of Strongyloides infection was highly related to geo-climatic type of area, but not to gender and age groups. (Med J Indones 2001; 10: 174-7)Keywords: Strongyloides stercoralis, geo-climatic condition, modifed Harada Mori technique, rural population, Bali
Fauna Nyamuk Aedes dan Kemungkinan Perannya dalam Penularan Demam Berdarah Dengue di Banjar Graha Kerti dan Banjar Kerta Petasikan, Denpasar Sutisna, Putu
Jurnal Kedokteran YARSI Vol 20, No 2 (2012): MEI - AGUSTUS 2012
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v20i2.162

Abstract

Indonesia, seperti banyak negara di wilayah tropis dan subtropis lainnya, merupakan wilayah endemik penyakit Demam Berdarah Dengue (DHF). Di Kodya Denpasar, kasus DHF terus meningkat dari tahun ke tahun selama kurun 2002-2008. Penelitian cross sectional ini dilakukan di di Banjar Graha Kerti dan Banjar Kerta Petasikan, Kelurahan Sidakarya, Denpasar, dengan tujuan untuk mengetahui adanya larva nyamuk Aedes dan tingkat kepadatannya di rumah penduduk di kedua lokasi tsb. Semua rumah di kedua Banjar tsb diikutkan sebagai sampel penelitian. Semua tempat air yang ditemukan di dalam rumah diperiksa untuk kemungkinan adanya larva nyamuk dan ditetapkan speciesnya (Aedes, Culex dan Anopheles). Dari 262 rumah, didapatkan sebanyak 869 tempat air tergenang dan 68 di antaranya mengandung larva Aedes, terdiri dari 37Aedes aegypti, 14A. albopictus, dan campuranA. aegypti danA. albopictus. House Indexuntuk Aedes adalah 17.2% danBruteau Indexuntuk Aedes adalah 20.6%, yang menunjukkan bahwa Aedes di Br Graha Kerti dan Banjar Kerta Petasikan mempunyai potensiuntuk menularkankasus-kasus DHF. Disarankan agar pelaksana program pengendalian kasus DHF di Kota Denpasar mengenali sifat-sifat biologis dari Aedes, termasuk lokasinya dalam tempat-tempat air di rumah tangga.Indonesia, seperti banyak negara di wilayah tropis dan subtropis lainnya,merupakan wilayah endemik penyakit Demam Berdarah Dengue (DHF). DiKodya Denpasar, kasus DHF terus meningkat dari tahun ke tahun selamakurun 2002-2008. Penelitian cross sectional ini dilakukan di di Banjar GrahaKerti dan Banjar Kerta Petasikan, Kelurahan Sidakarya, Denpasar, dengantujuan untuk mengetahui adanya larva nyamuk Aedes dan tingkatkepadatannya di rumah penduduk di kedua lokasi tsb. Semua rumah di keduaBanjar tsb diikutkan sebagai sampel penelitian. Semua tempat air yangditemukan di dalam rumah diperiksa untuk kemungkinan adanya larva nyamukdan ditetapkan speciesnya (Aedes, Culex dan Anopheles). Dari 262 rumah,didapatkan sebanyak 869 tempat air tergenang dan 68 di antaranyamengandung larva Aedes, terdiri dari 37Aedes aegypti, 14A. albopictus, dancampuranA. aegypti danA. albopictus. House Indexuntuk Aedes adalah17.2% danBruteau Indexuntuk Aedes adalah 20.6%, yang menunjukkanbahwa Aedes di Br Graha Kerti dan Banjar Kerta Petasikan mempunyai potensiuntuk menularkankasus-kasus DHF. Disarankan agar pelaksana programpengendalian kasus DHF di Kota Denpasar mengenali sifat-sifat biologis dariAedes, termasuk lokasinya dalam tempat-tempat air di rumah tangga.
Taeniasis in Karangasem Regency, Bali Winianti, Ni Wayan; Herdiana M, Elsa; Agus Wijayanti, Mahardika; Sutisna, Putu; Kapti, I Nengah; Sudiarta, I Wayan
WMJ (Warmadewa Medical Journal) Vol 3, No 1 (2018): May 2018
Publisher : Warmadewa University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/wmj.3.1.651.1-5

Abstract

Abstract Taeniasis is an infectious disease caused by the tapeworm Taenia saginata (beef tapeworm), Taenia solium (pork tapeworm), and Taenia asiatica (Asian tapeworm). Purpose of the study was to determine the relationship between meat consumption, individual characteristics, and environmental factors with taeniasis. Case control study was used to identify taeniasis among two groups: people living at Datah village (personal livestock) and people living at Karangasem village (obtained their meat from traditional market or slaughterhouse). Each group consisted of 98 people. Two subjects from the case group were positive for taenia egg both by direct smear and Willy?s Malory floatation methods. Proportion of taeniasisinthe case group was 2.04%. The type of meat consumed, methods of meat processing, frequency of consumption, ways of obtaining meat, individual characteristics, and environmental factors which may contribute to Taenia infection could not be evaluated due to limited number of positive cases. Abstrak Taeniasis merupakan penyakit infeksi yang disebabkan karena adanya cacing pita Taenia saginata (beef tapeworm), Taenia solium (pork tapeworm), and Taenia asiatica (Asian tapeworm)di dalam tubuh manusia. Tujuan penelitian ini untuk mengetahui hubungan antara konsumsi daging, karakteristik individu dan faktor lingkungan dengan kejadian taeniasis. Jenis rancangan penelitian yang digunakan yaitu case control antar 2 kelompok (penduduk di Desa Datah dan Kelurahan Karangasem, Kabupaten Karangasem, Bali). Kelompok kasus yaitu penduduk Desa Datah yang memenuhi kriteria kasus (mengkonsumsi daging dari hewan ternak pribadi), sedangkan kelompok kontrol yaitu penduduk di Kelurahan Karangasem yang memenuhi kriteria untuk kontrol (mengkonsumsi daging dari membeli di pasar atau tempat pemotongan hewan). Tiap kelompok terdiri dari 98 orang. Metode pemeriksaan feses dengan metode langsung dan pengapungan. Terdapat 2 orang yang positif taeniasis dari kelompok kasus, dengan ditemukan telur Taenia sp. pada pemeriksaan langsung dan pengapungan. Hubungan jenis daging, cara pengolahan, frekuensi mengkonsumsi dan cara memperoleh daging, karakteristik individu dan faktor lingkungan dengan kejadian taeniasis tidak dapat dianalisis secara statistic.