Endang Sutedja
Departemen Ilmu Kesehatan Kulit dan Kelamin Fakultas Kedokteran Universitas Padjadjaran/Rumah Sakit Dr. Hasan Sadikin Bandung

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Efikasi Terapi Kombinasi Salep Kalsipotriol 0,005% dan Klobetasol Propionat 0,05% Dibanding Klobetasol Propionat 0,05% pada Psoriasis Vulgaris

Majalah Kedokteran Bandung Vol 1, No 42 (2010)
Publisher : Majalah Kedokteran Bandung

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Abstract

Psoriasis adalah penyakit eritropapuloskuamosa kronik residif. Obat yang paling sering digunakan untuk terapi penyakit ini adalah kortikosteroid topikal, tetapi penggunaan jangka panjang dapat menyebabkan berbagai efek samping. Kalsipotriol merupakan obat topikal selain kortikosteroid yang memiliki efek samping yang lebih ringan. Tujuan penelitian ini adalah untuk membandingkan skor psoriasis area and severity index (PASI) sebelum dan sesudah pengobatan dengan kombinasi salep kalsipotriol 0,005% dengan salep klobetasol propionat 0,05% dan monoterapi salep klobetasol propionat 0,05% pada penderita psoriasis vulgaris. Skor psoriasis area severity index (PASI), digunakan untuk mengukur berat ringannya psoriasis dan mengevaluasi perbaikan lesi. Desain penelitian adalah uji klinik dengan rancangan acak secara double blind. Uji Mann-Whitney dan Wilcoxon digunakan untuk menganalisis data skor PASI sebelum dan sesudah pengobatan. Penelitian dilakukan di poliklinik I.K. Kulit danKelamin RS. Hasan Sadikin Bandung dari bulan November 2007- Januari 2008 dengan subjek penelitian sebanyak 44 orang yang dibagi dalam dua kelompok. Derajat keparahan penyakit dinilai dengan skor PASI yang dinilai sebelum, satu minggu, dan dua minggu setelah pengobatan. Kesimpulan: Penggunaan terapi kombinasi salep kalsipotriol 0,005% dan klobetasol propionat 0,05% selama dua minggu memiliki efikasi yang lebih baik dibandingkan monoterapi salep klobetasol propionat 0,05%, secara statistik sangat bermakna (p<0,001).Efficacy Combination Therapy of 0.005% Calcipotriol and 0.05%Clobetasol Propionat Compared to 0.05% Clobetasol Propionat Ointment in Psoriasis VulgarisPsoriasis is a chronic relapsing erythropapulosquamous skin disease. Topical corticosteroid is the most common treatment for psoriasis, but it can cause many side effect on longterm used. Calcipotriol ointment is the other topical treatment for psoriasis and has minimal side effect. The study was a double blind randomized clinical trial, compared psoriasis area and severity index score before and after treatment combination therapy of 0.005% calcipotriol ointment 0.05% and clobetasol propionat ointment with 0.05% clobetasol propionat alone. Psoriasis area severity index (PASI) score was used to evaluate severity degree and imprvoment of psoriasis. Mann-Whitney and Wilcoxon test were used for statistical analysis of PASI score before and after treatment. Study was conducted at Dermatology and Venereology outpatient Department of Hasan Sadikin Hospital Bandung period November 2007 to January 2008 with fourty four psoriasis vulgaris patients were enrolled in this study, divided into two groups. The assesment of the severity of skin lesions psoriasis area and severity index was performed at baseline, after one week and two weeks treatment. Conclusion: the use of daily 0.005% calcipotriol ointment combined with 0.05% clobetasol propionate

Penurunan Aktivitas Tirosinase dan Jumlah Melanin oleh Fraksi Etil Asetat Buah Malaka (Phyllantus emblica) pada Mouse Melanoma B16 Cell-Line

Majalah Kedokteran Bandung Vol 45, No 2 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Akumulasi melanin menyebabkan hiperpigmentasi dan bila terdapat pada wajah dapat menimbulkan masalah psikososial. Bahan depigmentasi dari tanaman semakin banyak digunakan. Bahan yang dikembangkan harus efektif menghambat sintesis melanin serta tidak toksik terhadap melanosit. Penelitian ini bertujuan menguji efek fraksi etil asetat buah Phyllanthus emblica (P. emblica) atau buah malaka, terhadap sintesis melanin dengan mengukur jumlah melanin dan aktivitas tirosinase, yang merupakan enzim utama sintesis melanin, secara spektrofotometrik pada kultur sel melanosit mouse melanoma B16 cell-line. Sitotoksisitas diukur dengan metode 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Penelitian dilakukan pada November–Desember 2009 di Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, Korea Selatan. Hasil penelitian menunjukkan aktivitas tirosinase dan jumlah melanin menurun bergantung pada dosis perlakuan berbagai konsentrasi fraksi etil asetat buah P. emblica dengan inhibition concentration (IC) 50 berturutturut 95,63 dan 16,90 μg/mL, serta lethal dose (LD) 50 pada konsentrasi 106,64 μg/mL. Simpulan, fraksi etil asetat buah P. emblica mempunyai potensi sebagai bahan depigmentasi, karena mampu menurunkan sintesis melanin melalui inhibisi aktivitas tirosinase. [MKB. 2013;45(2):118–24]Kata kunci: Aktivitas tirosinase, etil asetat, melanin, Phyllanthus emblicaReduction of Tyrosinase Activity and Melanin Amount by Ethyl Acetate Fraction from Malaka (Phyllanthus emblica) Fruit in Mouse Melanoma B16 Cell-LineMelanin accumulation can lead to hyperpigmentation, and if it occurs on the face can cause psychosocial problem. Depigmenting agents derived from plants are increasingly utilized. Agents being developed have to be effective in inhibiting melanin synthesis and should not be toxic to melanocyte. This study aimed was to examine the effect of ethyl acetate fraction from Phyllanthus emblica (P. emblica) fruit, also known as malaka fruit, towards melanine synthesis, which was measured from the melanin amount and tyrosinase activity, the key regulatory enzyme in melanin synthesis, spectrophotometrically towards melanocytes of mouse melanoma B16 cell-line. The cytotoxic effect towards melanocytes was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. This study was conducted on November−December 2009 in Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, South Korea. The result of this study showed that tyrosinase activity and melanin amount decreased in a dose-dependent manner towards various concentrations of ethyl acetate fraction of P. emblica fruit with inhibition concentration (IC) 50=95.63 and 16.90 μg/mL, respectively and lethal dose (LD) 50 concentration 106.64 μg/mL. In conclusion, ethyl acetate fraction of P. emblica fruit is a potential depigmenting agent, since it can reduce melanin synthesis by inhibition of tyrosinase activity. [MKB. 2013;45(2):119–24]Key words: Ethyl acetate, melanin, Phyllanthus emblica, tyrosinase activity DOI: http://dx.doi.org/10.15395/mkb.v45n2.115

Methicillin-Resistant Staphylococcus aureus pada Penderita Dermatitis Atopik dan Sensitivitasnya terhadap Mupirosin Dibandingkan dengan Gentamisin

Majalah Kedokteran Bandung Vol 45, No 1 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) mulai dijumpai pada penderita dermatitis atopik (DA) derajat sedang hingga berat dan bersifat multiresisten terhadap antibiotik topikal. Gentamisin telah digunakan secara luas, sedangkan mupirosin merupakan pilihan utama eradikasi MRSA. Penelitian ini bertujuan untuk mengetahui kolonisasi MRSA pada penderita DA dan sensitivitasnya terhadap mupirosin dibandingkan dengan gentamisin di Poliklinik Departemen Ilmu Kesehatan Kulit dan Kelamin Rumah Sakit Umum Pusat Dr. Hasan Sadikin Bandung. Survei potong lintang analitik dilakukan selama 19 September–31 Oktober 2011 terhadap 22 penderita DA derajat sedang hingga berat sesuai dengan Scoring Atopic Dermatitis objektif dan 22 individu sehat. Seluruh subjek dilakukan pemeriksaan apusan kulit untuk deteksi MRSA dan uji sensitivitas terhadap mupirosin dan gentamisin. Pada kedua kelompok masing-masing didapatkan 10 laki-laki dan 12 perempuan. Pada kelompok penderita DA didapatkan DA derajat sedang (10/22) dan berat (12/22), dengan lesi akut (14/22) dan kronik (8/22). Pertumbuhan S. aureus didapatkan pada 17/22 penderita DA dengan sensitivitas terhadap mupirosin 14/17 dan gentamisin 11/17 penderita. Pada individu sehat tidak didapatkan pertumbuhan koloni S. aureus. Galur MRSA ditemukan pada 3/17 penderita DA dan dianggap berbeda secara bermakna bila dibandingkan dengan individu sehat (p<0,01), galur ini bersifat sensitif terhadap mupirosin pada 2/3 penderita dan seluruhnya resisten terhadap gentamisin. Simpulan, kolonisasi MRSA perlu dicurigai pada penderita DA derajat sedang hingga berat, sehingga perlu pengobatan tambahan antibiotik topikal mupirosin. [MKB. 2013;45(1):36–44]Kata kunci: Dermatitis atopik, gentamisin, MRSA, mupirosinMethicillin-Resistant Staphylococcus aureus in Atopic Dermatitis Patients and Its Sensitivity to Mupirocin Compared to GentamycinMethicillin-resistant Staphylococcus aureus (MRSA) is found in moderate to severe atopic dermatitis (AD) and is multiresistant against topical antibiotic. Gentamycin is widely used while mupirocin is the first line therapy to eliminate MRSA. This research is intended to observe the colonization of MSRA case in AD patients and its sensitivity to mupirocin compared to gentamycin in Dermato-venereology Clinic Dr. Hasan Sadikin General Hospital Bandung. An analytical cross sectional survey was intended from 19th September–31st October 2011 in 22 moderate to severe AD patients based on objective Scoring Atopic Dermatitis and 22 healthy persons. All subjects went through skin-scraping, MSRA was detected and sensitivity test was done for mupirocin and gentamycin. The subjects consisted of 10 boys and 12 girls in both group. Based on severity, 10/22 of AD subjects were in moderate AD and 12/22 were severe AD, 14/22 of AD subjects presented with acute lesions and 8/22 subjects had chronic lesions. In the group of AD patients, 17/22 growth of S. aureus were found with sensitivity against mupirocin 14/17 and gentamycin 11/17. In healthy individual group, no  S. aureus growth was found. Methicillin-resistant Staphylococcus aureus strain in AD patients was observed on 3/17 patients, and this was declared significantly different compared to healthy individuals (p <0.01). The isolates in 2/3 patients were sensitive to mupirocin and all patients were resistant to gentamycin. In conclusion, the patients of moderate to severe AD are prone to MRSA colonization and therefore requires mupirocin topical antibiotic treatment.  [MKB. 2013;45(1):35–43]Key words: Atopic dermatitis, gentamycin, MRSA, mupirocin DOI: http://dx.doi.org/10.15395/mkb.v45n1.203

Validitas Hasil Pemeriksaan Skin Prick Test terhadap Imunoglobulin E RAST Tungau Debu Rumah dan Debu Rumah pada Dermatitis Atopik

Majalah Kedokteran Bandung Vol 44, No 3 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Pada dermatitis atopik (DA) tipe ektrinsik terdapat peningkatan imunoglobulin E (IgE) spesifik terhadap tungau debu rumah (TDR) dan debu rumah (DR) dengan gambaran skin prick test (SPT) positif. Metode penelitian observasional analitik dengan rancangan studi potong lintang, bertujuan untuk mengetahui validitas hasil pemeriksaan SPT terhadap IgE radioallergosorbent testing (RAST) TDR dan DR pada penderita DA, supaya SPT dapat diaplikasikan untuk mendeteksi DA. Penelitian dilakukan selama Agustus–November 2011 di Poliklinik Departemen Ilmu Kesehatan Kulit dan Kelamin RS Dr. Hasan Sadikin Bandung. Uji statistik menggunakan Spearman’s rank correlation. Hasil penelitian menunjukkan SPT TDR yang positif pada DA lebih tinggi dibandingkan dengan non-DA secara statistik bermakna, sedangkan SPT DR yang positif pada DA lebih tinggi dibandingkan dengan non-DA secara statistik tidak bermakna. Nilai positivitas SPT dan IgE RAST terhadap TDR pada DA berbanding lurus, namun tidak terhadap DR. Nilai positivitas SPT TDR pada DA lebih tinggi (15/16) daripada non-DA (5/16) dan nilai positivitas SPT TDR berbanding lurus dengan IgE RAST TDR pada DA dengan Spearman’s rank correlation coefficient 0,541 (p=0,031). Hasil pemeriksaan SPT TDR terhadap IgE RAST TDR pada kelompok DA memberikan validitas yang lebih tinggi dengan sensitivitas 100%, spesifisitas 25%, dan akurasi 81%, dibandingkan SPT DR terhadap IgE RAST DR dengan sensitivitas 43%, spesifisitas 78%, dan akurasi 63%. Simpulan, pemeriksaan SPT TDR dapat dijadikan patokan diagnosis DA, karena hasil pemeriksaan SPT TDR yang positif dapat memberikan arti peningkatan IgE spesifik dengan pemeriksaan IgE RAST TDR. [MKB. 2012;44(3):186–91].Kata kunci: Debu rumah, dermatitis atopik, IgE RAST, skin prick test, tungau debu rumahValidity of Skin Prick Test toward House Dust Mite and House Dust Immunoglobulin E RAST in Atopic DermatitisIn extrinsic type atopic dermatitis (AD) there is an increased of specific immunoglobulin (IgE) towards house dust mites (HDM) and house dust (HD), resulting in positive skin prick test (SPT). The method of this study was cross sectional, analytic-observational, to find validity of SPT toward HDM and HD IgE radioallergosorbent (RAST) in AD patients. The study was held from August to November 2011 in Department of Dermatovenereology Dr. Hasan Sadikin Hospital Bandung. Spearman’s rank correlation coefficient was used for statistical analysis. The results showed positive SPT to HDM in AD patients were statistically significant higher than non-AD. Positive SPT to HD in AD patients were higher than non-AD but statistically non significant. Value of HDM SPT positivity and IgE RAST in AD was directly proportional, but not with HD. There was a higher positivity of HMD SPT in AD patients (15/16) than in non-AD (5/16). Positivity value of HDM SPT was proportional to the HDM IgE RAST in AD patients with Spearman’s Rank correlation coefficient 0.541 (p=0.031). The results of HDM SPT toward HDM IgE RAST in AD patients showed higher validity sensitivity, specificity, and accuracy, 100%, 25% and 81% respectively, while HD SPT toward HD IgE RAST with sensitivity, specificity, and accuracy 43%, 78% and 63% respectively. In conclusions, HDM TDR examination could be used as a marker for AD diagnosis, due to positive HDM TDR examination result could give a meaning of increased specific IgE with RAST IgE examination. [MKB. 2012;44(3):186–91].Key words: Atopic dermatitis, house dust, house dust mites, IgE RAST, skin prick test DOI: http://dx.doi.org/10.15395/mkb.v44n3.207

PENGEMBANGAN FORMULA DAN EVALUASI GEL REJUVENASI DARI ASCORBYL GLUCOSIDE MELALUI SISTEM PENGHANTARAN TEKNOLOGI MACROBEAD® - Development and Evaluation Formula Gel Rejuvenation of Ascorbyl Glucoside Through Technology Delivery System Macrobead®

Indonesian Journal of Applied Sciences Vol 4, No 1 (2014)
Publisher : Universitas Padjadjaran

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Abstract

AbstrakVitamin C dan derivatnya banyak digunakan dalam sediaan kosmetika sebagai pencerah kulit. Pada penelitian ini akan dikembangkan gel rejuvenasi yang mengandung derivat vitamin C ascorbyl glucoside (AA2G) dalam sistem penghantaran macrobead®. Selain sebagai pencerah kulit, AA2G juga dapat dipakai untuk pengelupasan dan peremajaan kulit (rejuvenasi). Persen jeratan ditetapkan terlebih dahulu dengan perbandingan (AA2G: macrobead®) 1:2, 2:2 dan 3:2, basis yang digunakan adalah acrylates copolymer (5%, 10%, 15%) dan alkyl acrylate crosspolymer (0,5%, 1%, 1,5%). Formulasi gel rejuvenasi dibuat dalam variasi konsentrasi AA2G yaitu 1%, 2% dan 3%. Uji efektivitas dilakukan terhadap 30 orang relawan dengan menggunakan punggung tangan bagian kanan. Uji difusi dilakukan selama 360 menit menggunakan sel difusi franz. Hasil penetapan persen jeratan menunjukan perbandingan 1:2 memiliki jeratan terbesar yaitu  88,60%. Basis alkyl acrylate crosspolymer dipilih sebagai basis paling stabil dengan konsentrasi 1%. Hasil penelitian menunjukan tangan yang diolesi formula gel terpilih (1:2) menunjukan perubahan terhadap kulit yaitu kulit lebih cerah, lebih kencang dan lebih halus dibandingkan tangan kiri sebagai kontrol.  Hasil uji difusi menunjukan bahwa formulasi gel rejuvenasi dapat melintasi membran sebesar 0,12% dalam waktu 360 menit.Kata kunci: ascorbyl glucoside, acrylates copolymer, alkyl acrylate crosspolymer, macrobead®, rejuvenasiAbstractVitamin C and its derivatives are widely used in cosmetics as a skin lightening preparations. This research will be developed rejuvenation gel containing vitamin C derivative ascorbyl glucoside (AA2G) in macrobead® delivery system. Beside as a skin lightening, AA2G can also be used for exfoliation and skin rejuvenation. Percent entrapment set beforehand by comparison (AA2G : macrobead®) 1:2, 2:2 and 3:2, the base used is acrylates copolymer (5%, 10%, 15%) and alkyl acrylate crosspolymer (0.5%, 1.0%, 1.5%). Rejuvenation gel formulation is made in various concentration of AA2G is 1%, 2% and 3% . Effectiveness test is carried out on 30 volunteers by using the back of the right hand. Diffusion test is performed for 360 minutes using a Franz diffusion cell. Determining percent entrapment results showed 1:2 ratio has the biggest trap that is 88.60%. Base alkyl acrylate crosspolymer selected as the most stable base with a concentration of 1%. The results showed that smeared hand gel formula selected (1:2) showed changes in the skin is skin brighter, firmer and smoother than the left hand as a control. The test results showed that the diffusion gel formulation of rejuvenation can traverse the membrane of 0.12% within 360 minutes.Key word : ascorbyl glucoside, acrylates copolymer, alkyl acrylate crosspolymer, macrobead®, rejuvenasi

Etiology of Allergic Contact Dermatitis based on Patch Test

Althea Medical Journal Vol 4, No 4 (2017)
Publisher : Althea Medical Journal

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Abstract

Background: Allergic contact dermatitis is a type of contact dermatitis, whose  prevalence tends to increase every year. Patch test is an important diagnostic test to confirm the disease. The result of patch test can help doctors to perform appropriate treatment.  The aim of the study was to identify  the most frequent allergen causing allergic contact dermatitis based on patch test results.Methods: This was a descriptive retrospective study among patients who underwent patch test at the Dermatology and Venereology Clinic in Dr.Hasan Sadikin General Hospital Bandung, Indonesia from August 1st, 2014 to August 31st, 2015. The patch test result was obtained from the patient’s medical record and recapitulation data. The patient’s medical record would be collected based on inclusion and exclusion criteria. This study also collected demographic data of the patients, including age, sex, and occupation.The collected data were presented using tables.Results: Out of 45 patient’s medical records, 19 patients were male (42.2%) and 26 patients were female (57.8%). Nickel sulphate was the most frequent allergen with 17 sensitization. Thirteen out of 17 patients who were sensitized by nickel sulphate were females. A great majority of the patients were in the age group of 15–44 years. Most of the patients were students (42.2%). Allergic contact dermatitis (64.4%) was the main clinical diagnosis among the patients.Conclusions: The most frequent allergen causes allergic contact dermatitis is nickel sulphate.

A NOSOCOMIAL INFECTION MANIFESTED AS ERYSIPELAS IN PEMPHIGUS FOLIACEUS PATIENT UNDER INTRAVENOUS DEXAMETHASONE TREATMENT

Indonesian Journal of Tropical and Infectious Disease Vol 6, No 2 (2016)
Publisher : Institute of Topical Disease

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Abstract

Introduction: Puncture wound in diagnostic interventions permits the entry of bacteria into the skin or soft tissue, thus precipitating nosocomial infection, such as erysipelas. There are other risk factors of nosocomial infections including old age, immunosuppressive drugs, and underlying diseases. Pemphigus foliaceus (PF) is an autoimmune disease with corticosteroid treatment as the mainstay therapy, which could cause immunosuppression and predispose patients to infection. The objective of this paper was to report erysipelas as one of the manifestations of nosocomial infection in patients under immunosuppressive therapy. Case: A case of erysipelas acquired on the 9th day of hospitalization in a PF patient underwent intravenous dexamethasone injection, with history of puncture wounds on the previous day on the site of erysipelas was reported. The clinical findings of erysipelas were well defined, painful erythema and edema that felt firm and warm on palpation, with blisters and pustules on top. Gram staining from the pustules and blisters fluid revealed Gram (+) cocci. Patient was given 2 grams intravenous ceftriaxone for 7 days and saline wet compress. Improvement on the erysipelas was seen the day after ceftriaxone injection. The patient was discharged after 12 days of hospitalization with improvement both on the PF and the erysipelas. On the next visit 7 days later, the erysipelas lesion disappeared. Conclusion: Puncture wound and immunosuppresive treatment are the factors that could cause erysipelas as a nosocomial infection, and an appropriate treatment of the infection would decrease the functional disability of the patient.

The Positive Skin Prick Test not Correlate with Disease Severity and Quality of Life in Atopic Dermatitis Patients

Berkala Ilmu Kesehatan Kulit dan Kelamin Vol 29, No 3 (2017): DESEMBER
Publisher : Faculty Of Medicine Airlangga University

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Abstract

Background: Atopic dermatitis (AD) is a skin disease which cause stress to the patients. The chronic process of AD can cause physical, social, and psychological impairments. The severity of AD can also be affected by allergen exposures, which in turn will affect the quality of life of the patient. Skin prick test (SPT) can be used to evaluate allergen sensitization. Purpose: To evaluate correlation between SPT positivity to severity and quality of life of AD patients. Methods: The study was an observational cross-sectional study. Twenty five AD patients were recruited based on inclusion and exclusion criteria. Questionnaire was used to measure the quality of life of patients, and Scoring of Atopic Dermatitis (SCORAD) to measure the disease severity. Most of participants were women (80%), with median of age 26.84±13.71. Results: Positive SPT was obtained in 68% of patients, most of them were caused by house dust mites (55.2%). Seventy six percent of patients experienced mild AD, 12% moderate AD, and also 12% patients experienced severe AD. Significant correlation was observed between the severity and quality of life (p=0.001;r=0.617), while the SPT positivity has no correlation with the severity (p=0.912;r=-0.023) and quality of life (p=0.959;r=0.011). Conclusion: This study revealed that the severity of the disease has a correlation with quality of life, but SPT positivity has no significant correlation with severity and quality of life.

Efek Penggunaan Probiotik Topikal Terhadap Ekspresi MMP-13 dan Kolagen III pada Lapisan Dermis Tikus yang Dipapar Sinar Ultraviolet-B

Indonesian Journal of Clinical Pharmacy Vol 5, No 2 (2016)
Publisher : Indonesian Journal of Clinical Pharmacy

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Abstract

Proses penuaan kulit terjadi karena kombinasi penurunan kapasitas proliferasi sel-sel kulit, berkurangnya sintesis matriks dermis, dan peningkatan ekspresi enzim yang mendegradasi matriks kolagen. Penelitian ini merupakan eksperimental laboratorik, menggunakan 24 ekor tikus jantan galur Sprague-Dawley sebagai objek penelitian. Pada kelompok perakuan, kulit dorsal kedua kelompok dicukur, kemudian diberikan paparan sinar UVB seminggu tiga kali selama 4 minggu dengan dosis total penyinaran 840 mJ/ cm2. Kulit kelompok perlakuan diolesi krim Lactobacillus plantarum FNCC 0020 yang tidak bereplikasi, sehari 2 kali dengan komposisi aplikasi topikal koloni 247,27x107, sedangkan kelompok kontrol tidak diolesi apapun. Hasil penelitian diperoleh, baik intensitas, distribusi, maupun histoskor MMP-13 antara kelompok perlakuan dan kelompok kontrol tidak menunjukkan ada perbedaan yang bermakna (p>0,05). Intensitas kolagen III baik pada kelompok perlakuan maupun kontrol semua mempunyai derajat kuat, sedangkan distribusi kepadatan kolagennya paling rendah 20–50% dan tertingginya di atas 80%. Perbandingan distribusi kepadatan kolagen III secara statistik sangat bermakna (p<0,01). Pemberian krim topikal L. plantarum FNCC 0020 meningkatkan ekspresi distribusi kepadatan kolagen III namun tidak menurunkan ekspresi MMP-13. Kata kunci: Lactobacillus plantarum, kolagen, (matrix metalloproteinases) MMP13 Effect of Topical Probiotic on MMP-13 and Collagen III Expression in The Dermis Layer of Male Rats Irradiated with Ultraviolet-B Abstract Nowadays, there is a big interest in the use of topical probiotic preparations for skin health. One of the probiotics therurapeutic benefits is used as anti-aging. During aging, there is stimulation of activator protein-1 (AP-1) which is a transcription factor that inhibits the production of collagen and AP-1 supports the breakdown of collagen by enzymes called matrix metalloproteinases (MMPs). As administration of oral Lactobacillus plantarum could inhibit skin aging by lowering the activity of MMP, so the collagen degradation can be derived so probably topical use of Lactobacillus plantarum may give more prominent effects. We used 24 male rats Sprague-Dawley strain as research objects. This study was divided into two groups, the treatment and control groups. The shaved dorsal skin of rats were irradiated with UVB three times a week for 4 weeks with total irradiation dose of 840 mJ/cm2. Skin cream, containing 247.27x107 CFU non-replicating Lactobacillus plantarum FNCC 0020, was smeared on the treatment group, two times daily, whereas the control group did not receive any treatment. Skin biopsies were done at the end of the study for examination of MMP-13 and collagen III expressions. Intensity, distribution, and histoscore of MMP-13 between the treatment and the control group showed no significant difference (p>0.05). The treatment group showed a significant different in the intensity of collagen III with the density distribution of 20–50% and the highest density was 80% (p<0.01). Administration of topical cream L. plantarum FNCC 0020 increased the expression of collagen III density distribution, but not the MMP-13 expression.Keywords: Collagen, Lactobacillus plantarum, (matrix metalloproteinases) MMP13

AKTIVITAS DEPIGMENTASI FRAKSI N-HEKSANA BUAH MALAKA (PHYLLANTHUS EMBLICA) PADA SEL MELANOSIT MOUSE MELANOMA B16

Indonesian Journal of Applied Sciences Vol 3, No 1 (2013)
Publisher : Universitas Padjadjaran

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Abstract

AbstractInhibitor of melanin synthesis can be used as depigmenting agent to treat skin hyperpigmentation. Herbal depigmenting agents are increasingly utilized due to their character in inhibiting melanin synthesis without causing melanocyte toxicity. This study was aimed to examine depigmenting activity of n-hexane fraction of Phyllanthus emblica fruit by measuring its melanin synthesisinhibition on melanocyte mouse melanoma B16 cell line. Melanin synthesis inhibition was measured spectrophotometrically by counting the melanin and the activity of tyrosinase. The cytotoxicity of n-hexane fraction on melanocytes was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. This study was conducted on November-December2009 at Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, South Korea. The result of this study indicated that melanin count and tyrosinase activity decreased in a dose-dependent manner of n-hexane fraction of P. emblica fruit (IC50 31.68 and 77.92 μg/ml, respectively). The viable cells decreased with increasing concentration ofthe n-hexane fraction of P. emblica fruit (LD50 87.39 μg/ml).Key words: n-hexane fraction, Phyllanthus emblica, depigmenting agent

Co-Authors - Setiawan, - - Sudigdoadi, - Abdassah, Marline Abdassah Achadiyani Achadiyani, Achadiyani Achmad Yudha Pranata, Achmad Yudha Agustina, Rohana Anas Subarnas Anggraini, Dita Maulida Anissa, Anissa Aseng Ramlan Dany Hilmanto Dasim Budimansyah Devi Nursjamsi, Nadilla Carissa DEWI HERAWATI Diah Dhianawaty Dian Indriyani DLH, Dzul?kar Dwi Prasetyo Effendi, Jusuf Eka, Astuti Emma Surachman, Emma Surachman Eva Susanti Evi Sri Suryani Firman Fuad Wirakusumah Firman Wirakusumah, Firman Hadyana Sukandar Handayani, Retty Hartati Purbo Dharmadji, Hartati Purbo Hartati Purbo Dharmaji, Hartati Purbo Hendra Gunawan Herman Susanto Husin, Farid Idrajinata, Ponpon Iin Supartinah Noer Ike MP Siregar, Ike MP Judistianti, Tina Julianti, Mastiur Jusuf S Effendi Karlina, Intan Keni Istasaputri M. Kurniawati Kurniawati Lastri, Lastri Lesmana, Ronny Lilis Sumiati, Lilis Madjid, Tita Madjid, Tita Husnitawati Madjid8, Tita Husnitawati Marindani, Vani Marliza, Lenni Mose, Johanes MUCHTAN SUJATNO Mulyati, Iceu Noegroho, Bambang Nugrahini, Evi Nur Hasanah Nurhalim Shahib Nurhasanah Nurhasanah Oki Suwarsa Pangastuti, Miranti Ponpon Idjradinata Ponpon S Idjradinata Ponpon S. Idjradinata Pri Hastuti, Pri Purwana, Hasan Purwara, Benny Reiva Farah Dwiyana, Reiva Farah Reti Hindritiani Rovina Ruslami, Rovina Setiawan Setiawan Setiawati, Elsa P Sianipar, Imelda M.G Sormin, Erfina Rohana Sunarjati Sudigdoadi Tarawan, Vita M. Tina Dewi Judistiani Winarni, Lastri Yeyen Yovita Mulyana Yoni Syukriani, Yoni Yuni Susanti Pratiwi, Yuni Susanti