I-K Sutama
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Effectivity of various concentration of lactose in Tris extender on liquid semen viability of Saanen bucks Tambing, Surya Natal; Sutama, I-K; Arifiantini, R.I
Jurnal Ilmu Ternak dan Veteriner Vol 8, No 2 (2003): JUNE 2003
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.484 KB) | DOI: 10.14334/jitv.v8i2.377

Abstract

The objective of this study is to determine the best concentration of lactose in Tris extender in maintaining liquid semen viability of Saanen bucks stored at 5°C. Four heads of Saanen bucks of 2-4 years old were used as semen source. Semen was collected once a week using an artificial vagina. Variance analysis was conducted based on completely randomized design with three treatments, i.e 30, 60, and 90 mM lactose (L30, L68 and L90). Duncan test was used to observe the different between treatment. Results indicated that the mean percentage of motility, live sperm, intact plasma membrane, and intact acrosomal cap of liquid semen in treatment L30 (70.83; 78.20; 79.56 and 81.20% respectively) were significantly higher (P<0.05) than treatment L60 (67.08; 73.73; 75.01 and 76.87% respectively) and treatment L90 (61.50; 71.88; 69.08 and 71.33% respectively). After storage for 48 hours at 5°C apparently the mean percentage of motility, live sperm, intact plasma membrane and intact acrosomal cap in treatment L30 were significantly higher (P<0.05) than those of in treatment L60 and L90. Decreasing percentage of motility, live sperm, intact plasma membrane, and intact acrosomal cap during storage at 50C from 0 until 48 hour in treatment L30 (42.83; 19.75; 40.84 and 39.00% respectively) were significantly lower (P<0.05) than those of in treatment L60 (55.41; 23.06; 50.14; and 50.90% respectively) and treatment L90 (54.83; 29.93; 50.14 and 50.97% respectively). It was concluded that supplementation 30 mM lactose in Tris extender could maintain liquid semen viability in Saanen bucks.   Key words: Saanen bucks, lactose, liquid semen
Effect of pregnant mare serum gonadotrophin injection on litter size in young Etawah-cross does Artiningsihi, N.M; Purwantara, B; Achjadi, R.K; Sutama, I-K
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 1 (1996)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (581.164 KB) | DOI: 10.14334/jitv.v2i1.38

Abstract

The incidence of twins and/or multiple births in 20 heads of young Etawah-cross does was studied following oestrous synchronization using intravaginal sponges containing 60 mg medroxyprogesterorle acetate (Repromap) for 15 days . Twenty four hours priorto sponges withdrawal, the does were injected with pregnant mare serum gonadotrophin (PMSG) at dose rates of 0 (Group A), 10 (Group B), 15 (Group C) and 20 iu/kg (Group D) body weight. Amature buck fitted with an apron was used to detect the onset of oestrus at every four hours. The oestrous doe was naturally mated twice, 12 hours after onset of oestrus and 10 hours later. About 3-5 days after oestrus, all does were subjected to mid-ventral laparoscopy to detect ovulation rate . Two months after mating all does were subjected to pregnancy test using diagnostic ultrasonography. Results showed that all does exhibited clear sign of oestrus. The onset of oestrus occurred 15-43 hours after sponges withdrawal or 39-59 hours after PMSG injection. Does injected with PMSG (Groups B, C and D) showed oestrus 16-21 hours earlier (P<0.05), and it was 1 .6-4.8 hours longer (P>0 .05) than that of control (Group A) . However, there was no significant differences among the PMSG-treated groups . Ovulation rates increased from 1.0 in Group Ato 1.8 in Group B and 2.6 in bah Groups Cand D. Average litter size in Groups A, B, C and D were 1.0, 1.8, 2.4 and 1.0, respectively. It was concluded that injection of 15 iu PMSG/kg body weight gave the best result for increasing litter size in young Etawah-cross does .   Key words: Etawah-cross, PMSG, synchronization, reproduction
Effect of pregnant mare serum gonadotrophin injection on litter size in young Etawah-cross does Artiningsihi, N.M; Purwantara, B; Achjadi, R.K; Sutama, I-K
Indonesian Journal of Animal and Veterinary Sciences Vol 2, No 1 (1996)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (581.164 KB) | DOI: 10.14334/jitv.v2i1.38

Abstract

The incidence of twins and/or multiple births in 20 heads of young Etawah-cross does was studied following oestrous synchronization using intravaginal sponges containing 60 mg medroxyprogesterorle acetate (Repromap) for 15 days . Twenty four hours priorto sponges withdrawal, the does were injected with pregnant mare serum gonadotrophin (PMSG) at dose rates of 0 (Group A), 10 (Group B), 15 (Group C) and 20 iu/kg (Group D) body weight. Amature buck fitted with an apron was used to detect the onset of oestrus at every four hours. The oestrous doe was naturally mated twice, 12 hours after onset of oestrus and 10 hours later. About 3-5 days after oestrus, all does were subjected to mid-ventral laparoscopy to detect ovulation rate . Two months after mating all does were subjected to pregnancy test using diagnostic ultrasonography. Results showed that all does exhibited clear sign of oestrus. The onset of oestrus occurred 15-43 hours after sponges withdrawal or 39-59 hours after PMSG injection. Does injected with PMSG (Groups B, C and D) showed oestrus 16-21 hours earlier (P<0.05), and it was 1 .6-4.8 hours longer (P>0 .05) than that of control (Group A) . However, there was no significant differences among the PMSG-treated groups . Ovulation rates increased from 1.0 in Group Ato 1.8 in Group B and 2.6 in bah Groups Cand D. Average litter size in Groups A, B, C and D were 1.0, 1.8, 2.4 and 1.0, respectively. It was concluded that injection of 15 iu PMSG/kg body weight gave the best result for increasing litter size in young Etawah-cross does .   Key words: Etawah-cross, PMSG, synchronization, reproduction
Reproductive and productive efficiencies of Etawah Grade goats under various mating managements Sunadi, Bambang; Sutama, I-K; Budiarsana, I-G.M
Indonesian Journal of Animal and Veterinary Sciences Vol 2, No 4 (1998)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.438 KB) | DOI: 10.14334/jitv.v2i4.77

Abstract

Thirty six Etawah Grade (PE) goats were treated with three type of mating managements, i.e. mated at the first oestrous (A), mated at the second oestrous (B), and mated at the third oestrous (C) after parturition, respectively . Results showed that average first estrous was 56 days (26-99 d) after parturition with estrous cycle of 21 days . Conception rate at the first and second oestrous mating managements (A and B) were 50 and 70%, respectively . Variability of birth weight (3,4 - 3,5 kg) under three mating managements were not significantly different (P>0 .05), but the weaning weight of kids of B (16 .4 kg) was higher (P<0.05) than A (11 .8 kg) and C (12.9 kg), respectively. Does productivity (total weaning weight) was not significantly affected by mating management, i.e. at fisrt, second or third oestrous after parturition .   Keywords : Goats, mating management, productivity
Production response of Etawah cross breed (PE) doe due to improvement of feeding management during late pregnancy and lactation period Yulistiani, Dwi; Mathius, I-W; Sutama, I-K; Adiati, Umi
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 2 (1999)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.313 KB) | DOI: 10.14334/jitv.v4i2.143

Abstract

An experiment was caried out to study the protein requirement for Etawah cross breed. Thirty Etawah cross breed does were used in this study (average body weight 37.6+3.5 kg) and randomized to obtain one of three treatments. The treatments were the protein content of concentrate supplement. The protein levels were R1 (CP 16%), R2 (CP 22%) and R3 (CP 26%). The concentrate supplements were offered during late pregnancy and early (first 3 months) lactation period. The study showed that dry matter intake during pregnancy and early lactation period was not affected by treatments. The highest average daily gain during late pregnancy reached by R3 (66.45 g/day) which was not significantly different with R2 (61.9 g/day) and R1 (48.8 g/day). The highest total birth weight per does was achieved by R2 (6.05 kg). Average daily milk production was not affected by treatment. The production at first week of lactation was 1,044.5 g/day and decreased to 466.7 g/day in week 11. R2 produced the highest average daily gain (107.8 g/day) preweaning per does, while R1 and R3 was 84 and 84.4 g/day, respectively.   Key words : Etawah cross breed (PE), pregnancy period, lactation period, protein levels
The effect of continuous feeding of gliricidia on reproduction and production performances of Javanese Fat-Tailed sheep: Impact on the second breeding ., Supriyati; Budiarsana, I-G.M; Sutama, I-K
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 3 (1999)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.195 KB) | DOI: 10.14334/jitv.v4i3.153

Abstract

The effect of continuous feeding of gliricidia on reproduction and production performances in second breeding of Javanese Fat-Tailed (JFT) sheep through the second breeding period was studied. Twenty six ewes post lactation at first lambing (body weight 22.5-26.5 kg) were divided into four treatment groups. Each group consisted of 6 animals except Group D had 8 animals. They were given King grass (Pennisetum purpureophoides) and gliricidia (Gliricidia sepium Jacq.) leaf with ratio of 100:0 (Group A = control), 75:25 (Group B), 50:50 (Group C) and 0:100 (Group D). Forages were given 2.5-3% (dry matter) of liveweight. All groups were supplemented with 100g/head/day concentrate (crude protein = 16%), but during late pregnancy and lactation they were supplemented at 200 g/head/day. Results showed that feeding gliricidia 25-100% of total forages improved body weight due to the increasing protein intake. The maximum concentration of progesteron increased from 0.81 to 2.78 ng/ml. The ovulation rate and prolification also increased significantly (P<0.05) from 1.6 to 3.0 and 1.33 to 2.38, respectively. Individual birth and weaning weights of the lambs were not affected. It is concluded that feeding gliricidia continuously up to 100% as forages and supplemented with concentrate gives positive effect on bodyweight gain, reproduction and production performances in second breeding of JFT sheep.   Key words : Sheep, gliricidia, reproduction, second breeding
Dietary energy and crude protein requirements of Ettawah Cross Kids: 1. Intake, digestibility, availability and utilization of nutrients Mathius, I-W; Gaga, I.B; Sutama, I-K
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 2 (2002)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.735 KB) | DOI: 10.14334/jitv.v7i2.281

Abstract

An experiment was designed in order to study the crude protein and energy requirement of Ettawah Cross growing goats. Twenty seven kids (average body weight of 11.80 + 1.4 kg) were used and randomly allotted to a 3 x 3 factorial arrangement of treatments, consisting of three different levels of crude protein and three levels of energy. Results from this experiment showed that animal performance was effected by dietary treatments. Increasing levels of energy ration significantly (P<0.05) decreased the dry matter intake, with overall mean values were 3.0; 2.8 and 2.5% of body weight for low, medium and high levels of energy respectively. Dietary treatments increased energy (EM) and crude protein intake, and overall values were 0.2046 + 0.016 Mkal/kg BW0.75 and 8.20 + 2.473 g/kg BW0.75 repectively. Positive response on animal performance was also effected by dietary treatment, with overall mean value of 86.40 + 29.59 g head-1day-1. The highest response on animal performance (ADG 123.3 g) was found on kids fed diet containing combination of low level of energy and high level of crude protein. Meanwhile, the lowest average daily gain (45 g) was resulted by kids fed ration containing combination of high level of energy and low level of crude protein.   Key words: Protein-energy, etawah cross kids, growing fase
Effectivity of various concentration of lactose in Tris extender on liquid semen viability of Saanen bucks Tambing, Surya Natal; Sutama, I-K; Arifiantini, R.I
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 2 (2003)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.484 KB) | DOI: 10.14334/jitv.v8i2.377

Abstract

The objective of this study is to determine the best concentration of lactose in Tris extender in maintaining liquid semen viability of Saanen bucks stored at 5°C. Four heads of Saanen bucks of 2-4 years old were used as semen source. Semen was collected once a week using an artificial vagina. Variance analysis was conducted based on completely randomized design with three treatments, i.e 30, 60, and 90 mM lactose (L30, L68 and L90). Duncan test was used to observe the different between treatment. Results indicated that the mean percentage of motility, live sperm, intact plasma membrane, and intact acrosomal cap of liquid semen in treatment L30 (70.83; 78.20; 79.56 and 81.20% respectively) were significantly higher (P<0.05) than treatment L60 (67.08; 73.73; 75.01 and 76.87% respectively) and treatment L90 (61.50; 71.88; 69.08 and 71.33% respectively). After storage for 48 hours at 5°C apparently the mean percentage of motility, live sperm, intact plasma membrane and intact acrosomal cap in treatment L30 were significantly higher (P<0.05) than those of in treatment L60 and L90. Decreasing percentage of motility, live sperm, intact plasma membrane, and intact acrosomal cap during storage at 50C from 0 until 48 hour in treatment L30 (42.83; 19.75; 40.84 and 39.00% respectively) were significantly lower (P<0.05) than those of in treatment L60 (55.41; 23.06; 50.14; and 50.90% respectively) and treatment L90 (54.83; 29.93; 50.14 and 50.97% respectively). It was concluded that supplementation 30 mM lactose in Tris extender could maintain liquid semen viability in Saanen bucks.   Key words: Saanen bucks, lactose, liquid semen
Pre-weaning growth of Boer x Peranakan Etawah goats Kostaman, T; Sutama, I-K
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 2 (2005)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v10i2.462

Abstract

An experiment was conducted to study the growth patterns of Boer x Peranakan Etawah (PE) crossbred during pre-weaning period, at the Indonesian Research Institute for Animal Production, Ciawi-Bogor. Sixty-one kids were used in this experiment. They were reared with their mothers in group pen (3 x 4 m), with each pen contained 6-7 does. Does were fed of 2.5 kg freshlychopped King Grass and 0.7 kg concentrate head-1 day-1. Research results showed that birth weight of Boer x PE kids (group A) were significantly higher than those of PE kids (group B) (4.29 ± 0.63 vs 3.71 ± 0.89 kg/head, P<0.05). However, average preweaning daily weight gain (ADG) (116.40 ± 49.95 vs 105.29 ± 28.36 g/head, P>0.05) and weaning weight (14.64 ± 4.56 vs 13.30 ± 2.71 kg/head, P>0.05) were not significantly different between the groups. Pre-weaning mortality of the kids was relatively high in both group A (25%) and B (21,21%). While sex ratio (male : female) was 57.14 : 42.86% in group A and 51,52 : 48,48% in group B.     Key Words: Growth, PE, Boer Goat, Pre-Weaning
Efficacy of concentration of egg yolk in Tris extender with and without seminal plasma on frozen semen quality of Saanen bucks Tambing, Surya Natal; Sutama, I-K; Sariubang, M
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 4 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v13i4.576

Abstract

Bucks semen is easily damaged compared to bull semen during cryopreservation process. Consequently, frozen semen quality decrease especially motility, live sperm, intact plasma membrane, and intact acrosomal cap after thawing. Objectives of this research was to evaluate the effect of egg yolk concentration in Tris extender with and without seminal plasma in maintaing frozen semen quality of Saanen buck. Four heads of Saanen buck of 2-4 years old were used in this experiment. Semen was collected once a week using artificial vagina. Experimental design applied was factorial complete random desing 2x2, viz. A factor was seminal plasma (A1 = with seminal plasma and A2 = without seminal plasma), and B factor was concentration of egg yolk (B1 = 10% and B2 = 20%). Duncan test were applied to identity differences between treatment. Result of these study indicated that the mean percentage of motility (M), live sperm (LS), sperm with intact plasma membrane (IPM) and intact acrosomal cap (IAC) after dilution and equilibration were not significantly different (P>0.05) in all treatment. After thawing, the mean percentage of M, LS sperm with IPM and IAC in A1 treatment (41.43, 51.52, 56.63 and 50.35% respectively) were significantly higher (P<0.05) than A2 treatment (37.14; 48.67; 52.31 and 45.09% respectively). Likewise, the mean percentage of M, LS, sperm with IPM and IAC in B2 treatment (41.79; 51.32; 55.78 and 49.50% respectively) were significantly higher (P<0.05) than B1 treatment (36.79, 48.86; 53.16 and 45.94% respectively). There were a significant interaction between factors of seminal plasma and concentration egg yolk in Tris extender, where the increase of egg yolk concentration from 10% to 20% in unwashed seminal plasma treatment caused increase in percentages of M, LS, sperm with IPM and IAC. On the other hand in washed seminal plasma treatment there were a trend of decreasing frozen semen quality. It is concluded that the combination of 20% egg yolk in Tris extender with seminal plasma is effective in maintaining frozen semen quality of Saanen bucks. Key Words: Saanen Bucks, Semen Quality, Egg Yolk, Seminal Plasma