I GEDE KETUT SUSRAMA
Agricultural Pests and Diseases Department, Agroecotechnology Study Program, Faculty of Agriculture, Udayana University

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Uji Aktivitas Antimikroba Beberapa Ekstrak Bumbu Dapur terhadap Pertumbuhan Jamur Curvularia lunata (Wakk.) Boed. dan Aspergillus flavus LINK. SITEPU, IRMA SELVYANA Br.; SUADA, I KETUT; SUSRAMA, I GEDE KETUT
E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol. 1, No. 2, Oktober 2012
Publisher : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

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ABSTRACT The Antimicrobial Activity Test of Some Kitchen Seasoning Extracts on Growth of Fungus Curvularia lunata (Wakk.) Boed. and Aspergillus flavus LINK. This research was aimed to determine the ability of herbs extract in inhibiting the growth of fungus C. lunata and A. flavus and to determine the minimum inhibition concentration of each extracts as well. The results showed that the extracts could inhibit the growth of the two fungus. The most effective extract was turmeric against C. lunata and galangal extract against A. flavus with each inhibition was 38,6% and 26,6% respectively. The minimum inhibition concentration of all extracts were 0,5% on both C. lunata and A. flavus. Keyword : Antimicrobial, Kitchen seasoning, Curvularia lunata, Aspergillus flavus
Isolasi dan Identifikasi Agrobacterium tumefaciens dari Tanaman Wortel (Daucus carota L.) MANALU, YOLANDA HASSIAN; WIRAWAN, I GEDE PUTU; SUSRAMA, I GEDE KETUT
E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

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ABSTRACT Isolation and Identification of Agrobacterium tumefaciens from  Carrots (Daucus carota L.) Agrobacterium tumefaciens is a plant pathogenic bacterium that is widely used as a vector for inserting foreign genes into a plant chromosome to produce a transgenic plant. This bacterium cause a disease namely crown gall in dicotyledonous plants. This study is aim to isolate A. tumefaciens from local carrot (Daucus carota L.) by using some method such as selection media, characterization of the bacteria, and confirm by Koch’s postulates. The result of this study determined that carrot’s root taken from Bedugul area which showed crown gall symptom, an A. tumefaciens due to its characteristics e.g. shape of colonies, color, and growth of the bacteria in AB minimal medium that was a specific for A. tumefaciens.  By using the Koch’s postulates test showed that the isolated caused convex gall on the surface of carrot slice. DNA of isolated bacterium successfully electroforeted through agarose gel electrophoresis.  These results showed that the bacteria associated with carrot was A. tumefaciens. Keywords : A. tumefaciens, AB medium, Carrot, LB medium, Crown gall
Isolasi dan Identifikasi Agrobacterium Tumefaciens pada Tanaman Mawar (Rosa sp.). SILITONGA, NADIAH; WIRAWAN, I GEDE PUTU; SUSRAMA, I GEDE KETUT
E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

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ABSTRACT Isolation and Identification of Agrobacterium tumefaciens At Plant roses (Rosa sp.). Agrobacterium tumefaciens caused crown gall tumor in many of dicotyledonous plants. The purpose of this study tried to isolate and identify the A. tumefaciens from rose plant. Various techniques were used such as selection media, colonies shape and color, Kochs postulate test, DNA isolation and agarose gel elektroforesis. The results of this study showed that A. tumefaciens can be isolated through culturing in LB medium and selection AB medium that was specific for A. tumefaciens. The isolate caused crown gall tumor on carrot slice 3 weeks after inoculation by using a modified Koch’s postulate test. The characteristics of colony formed in this research are round shaped, cream coloured with pink tint, smooth edge, and convex elevation. DNA isolation and its running in agarose gel electroforesis showed positive result.   Key words : Agrobacterium tumefaciens, AB medium and Agarose gel electroforesis.
Identifikasi Fungi Mikoriza Arbuskular Secara Mikroskopis pada Rhizosfer Tanaman Alang-Alang (Imperata Cylindrica L.) di Desa Sanur Kaja NAINGGOLAN, ROMAULI THERESIA; WIRAWAN, I GEDE PUTU; SUSRAMA, I GEDE KETUT
E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.4, Oktober 2014
Publisher : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

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ABSTRACT Identification of Mikoriza Arbuskular Fungi in Microscopic the Rhizosphere of Reed (Imperatacylindric L.) in Sanur Kaja Village.   Mycorrhiza is a form of symbiosis between the fungus with a high level of plant (vascular plants, Tracheophyta), especially on the rooting. There is also a fungus with the other symbiotes, but the term mycorrhiza are typically for symbiotic plant roots which infect by fungus. This research aims to know the diversity of the genus or species of  mycorrhiza fungi arbuskular on the rhizosfer plant reeds in the village of Sanur Kaja, and to know whether or not there was colonization of mycorrhiza fungi arbuskular on the net root crops the reeds in the village of Sanur Kaja. The results of this research show that the symbiotes spores in the rhizosfer plant reeds is the genus Glomus multicaule spores, Glomus ambisporum, Acaulospora foveata, Gigaspora gigantae. Analysis on the plant roots mycorrhiza colonization reed showed a spherical structure called vesikular, while arbuskular is a structure on the hypha branches that resemble haustorium. (forming the pattern dikotom).   Key words: Acaulospora sp, Gigaspora sp, Glomus sp.
IDENTIFICATION OF ARBUSCULAR MYCORRHIZAL FUNGI IN RHIZOSPHERE SOIL OF SEVERAL GRASS SPECIES AND CACAO (Theobroma cacao L.) BASED ON ITS SPORE MORPHOLOGICAL CHARACTERISTICS Sintya Dewi, Ni Kadek; Susrama, I Gede Ketut; Sritamin, Made; Adnyana, Made Adnyana; Wirawan, I Gede Putu
International Journal of Biosciences and Biotechnology Vol 2, No 1, 2014
Publisher : International Journal of Biosciences and Biotechnology

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Arbuscular mycorrhizal fungi live in a symbiotic mutualism with plant roots and helps plant absorbnutrients and also able to live in various places. The study aimed to identify arbuscular mycorrhizalfungi in rizhosphere of several grass and cacao through microscopic method based on morphologicalcharacteristics and was conducted from December 2013 to March 2014. The methods used in this studywere spore isolation using wet sieving and root staining techniques. The results showed that arbuscularmycorrhizal fungi spores found in Imperata cylindrica L. were spores of Glomus (Glomales: Glomeaceae)with its vesicular and hyphae structure. The presence of spores and structures in Paspalum notatum wereAcaulospora (Glomales: Acaulosporaceae) and Gigaspora with its arbuscules, vesicules and hyphae structure.Spores and structures found in the Pennisetum purpureum were belong to genus of Glomus with hyphae andarbuscular structure. Spores and structures of mycorrhizal fungi in Cyperus rotundus are spores of the genusof Gigaspora (Glomales: Gigasporineae) and spores of Glomus with internal hyphae structure. While sporesand mycorrhizal structures in Cacao were found two types of spore belong to genus of Glomus with hyphaeand vesicular structures.
IDENTIFICATION OF ARBUSCULAR MYCORRHIZAL FUNGI IN RHIZOSPHERE SOIL OF SEVERAL GRASS SPECIES AND CACAO (Theobroma cacao L.) BASED ON ITS SPORE MORPHOLOGICAL CHARACTERISTICS Sintya Dewi, Ni Kadek; Susrama, I Gede Ketut; Sritamin, Made; Adnyana, Made; Wirawan, I Gede Putu
International Journal of Biosciences and Biotechnology Vol 2 No 1 (2014)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Arbuscular mycorrhizal fungi live in a symbiotic mutualism with plant roots and helps plant absorbnutrients and also able to live in various places. The study aimed to identify arbuscular mycorrhizal fungiin rizhosphere of several grass and cacao through microscopic method based on morphologicalcharacteristics and was conducted from December 2013 to March 2014. The methods used in this studywere spore isolation using wet sieving and root staining techniques. The results showed that arbuscularmycorrhizal fungi spores found in Imperata cylindrica L. were spores of Glomus (Glomales: Glomeaceae)with its vesicular and hyphae structure. The presence of spores and structures in Paspalum notatum wereAcaulospora (Glomales: Acaulosporaceae) and Gigaspora with its arbuscules, vesicules and hyphae structure.Spores and structures found in the Pennisetum purpureum were belong to genus of Glomus with hyphaeand arbuscular structure. Spores and structures of mycorrhizal fungi in Cyperus rotundus are spores of thegenus of Gigaspora (Glomales: Gigasporineae) and spores of Glomus with internal hyphae structure. Whilespores and mycorrhizal structures in Cacao were found two types of spore belong to genus of Glomus withhyphae and vesicular structures.
Identifikasi Mikoriza Vesikular Arbuskular (MVA) pada Rhizosfer Tanaman Ubi Jalar (Ipomoea batatas L.) dan Ubi Kayu (Manihot esculenta Crantz) serta Perbanyakannya dengan Media Zeolit WIDIATMA, PUTU SENA; WIRAWAN, I GEDE PUTU; SUSRAMA, I GEDE KETUT
E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.4, Oktober 2015
Publisher : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

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ABSTRACT Vesicular Arbucular Mycorrhizae (VAM) Identification of Sweet Potato (Ipomoea batatas L.) and Cassava (Manihot esculenta Crantz) Rhizosphere and Its Spore Multiplication in Zeolite Media Vesicular arbuscular mycorrhizae is an obligate symbiont that live in a symbiotic mutualism with plant roots and grow inside root cortical cells helping absorption of nutrients which necessary for plant growth. This research aimed to determine VAM species in sweet potato and cassava rhizosphere and also to determine effectiveness of zeolite media as a multiplication medium. The research has been conducted from October 2014 through December 2014. Spore isolation was done by conducting wet sieving method. Roots colonization percentages were calculated with gridline section method and spores multiplication through trapping culture method. Results showed that there were four VAM genera consist of 16 species from rhizosphere of both sample plants. Those four genera identified as Acaulospora (2 species), Gigaspora (1 species), Glomus (1 species), and Scutellospora (1 species) from rhizosphere of sweet potato and 3 genera identified as Acaulospora (3 species), Gigaspora (3 species), and Glomus (5 species) from rhizosphere of cassava. Colonization is characterized by special structure of VAM such as arbucule and vesicule in plant roots tissue. Inner spores of VAM were discovered in all three kind of plants used in this research. Spore multiplication with trapping culture method using zeolite media and corn as a symbionts can be considered as an effective method for VAM spore multiplication indicated by density increase of VAM spores. Keywords : Colonization, Cortical Cell, Inner Spore, Symbiont, Trapping Culture
Identifikasi Mikoriza Vesikular Arbuskular (MVA) dari Rhizosfer Tanaman Cabai (Capsicum annuum L.) dan Tomat (Solanum lycopersicum L.) serta Perbanyakannya Menggunakan Media Zeolit WIRAWAN, I WAYAN EKA ADI; SUADA, I KETUT; SUSRAMA, I GEDE KETUT
E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.4, Oktober 2015
Publisher : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

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ABSTRACT Vesicular Arbuscular Mycorrhizae (VAM) Identification of Chilli (Capsicum annuum L.) and Tomato (Solanum lycopersicum L.) Rhizosphere and Its Spore Multiplication in Zeolite Media Various attempts have been made to increase the production of chilli and tomato plants by farmers, including by using  inorganic fertilizer application on and on going basis. Considering potential problems that may occured due to inorganic fertilizer use, aplication of  biological fertilizer which one of them is containing vesicular arbuscular mycorrhizae (VAM) could be expected to assist the growth of chilli and tomato in more naturally manner. This study was aimed to determine genus and species of VAM in chilli and tomato rhizosphere, its colonization in root tissue, and to examine zeolite media compatibility with corn as a symbiont. Based on the results of the study, it was found four species namely Acaulospora fofeata, A. colombiana, A. Laevis, and Glomus ambisporum in chilli and four species in tomato that were identified as A. fofeata, A. colombiana, Scutellospora calospora, and G. ambisporum. Colonization were found in roots of chilli, tomato, and corn indicated by symbiotic structures arbuscules, vesicles, and inner spores. Zeolite media with corn as symbiotic plant is considered suitable for VAM spore propagation. Keywords: rhizosphere, Acaulospora fofeata, zeolite, inner spore
AGARWOOD PRODUCING FUNGAL INOCULANT FORMULATION IN KETIMUNAN TREE (Gyrinops versteegii DOMKE) Mega, I Made; Suanda, Dewa Ketut; Kasniari, Desak Nyoman; Susrama, I Gede Ketut
International Journal of Biosciences and Biotechnology Vol 3 No 1 (2015)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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This study aimed to find out the agarwood producing fungal inoculant formulation that infect Ketimunan tree (Gyrinops versteegii DOMKE) effectively and resulted in high quality of agarwood. Randomized Completely Block Design was applied in this study with formulation of Fusarium solani and Rhizopus sp inoculant in liquid and solid form were combined as treatments. Treatments were: solid Fusarium solani inoculant, solid Rhizopus sp inoculant, mixture of solid Fusarium solani inoculant and solid Rhizopus sp inoculant, liquid Fusarium solani inoculant, liquid Rhizopus sp inoculant, mixture of liquid Fusarium solani inoculant and liquid Rhizopus sp inoculant, and mixture of solid and liquid of Fusarium solani and Rhizopus sp inoculants. There are three replicates for each treatment. Parameters measured were level of fragrance, agarwood color, and its resin content. The result showed that all formulations affected resin content significantly compared to control of agarwood. Mixture of liquid Fusarium solani and liquid Rhizopus sp inoculant showed the best quality agarwood with quality characteristics: brownish black or agarwood with black and brownish black color, very strong fragrance, and with 13.58% resin content.
IN VIVO MULTISTEP MUTAGENESIS INDUCTION USING COLCHICINE ON COWPEA MUTANT 1 (Vigna unguiculata L. Walp) Susrama, I Gede Ketut; Wirawan, I Gede Putu
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.24843/IJBB.2018.v05.i02.p04

Abstract

In the previous research, we obtained three catagories of M1 mutant cowpea namely 1) purple pod mutant cowpea, 2) green pod mutant cowpea which has three pods in a stalk, and 3) green pod mutant cowpea which has two pods in a stalk. All those three catagories of M1 mutant cowpeas were treated again with colchicine in a multistep mutagenesis proses in vivo. Then, we found changes in number of leaflet in a petiole to 4 leaflets (quadrifoliate) and to five leaflets in a petiole (pentafoliate). These changes are indications that beside as a chromosome multipying alkaloid, colchicine causes other genetic changes as well. Through this mutagenesis research, we created gigas mutant cowpea that having longer leaves, longer pods and heavier seeds. The accurrence of two kinds of insect pests attack were found namely cowpea aphid Aphis craccivora and pod sucking bug Riptortus linearis.