Titik Susilowati
Program Studi Budidaya Perairan, Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

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Penambahan Air Kelapa Dan Gliserol Pada Penyimpanan Sperma Terhadap Motilitas Dan Fertilitas Spermatozoa Ikan Mas (Cyprinus Carpio L.) Kurniawan, Isnan Yudi; Basuki, Fajar; Susilowati, Titik
Journal of Aquaculture Management and Technology Vol 2, No 1 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh penambahan air kelapa dan gliserol terhadap fertilitas spermatozoa ikan mas (Cyprinus carpio L.) dan kondisi motilitasnya serta perlakuan yang terbaik pada penyimpanan sperma ikan mas strain Rajadanu. Percobaan ini menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan 3 kali ulangan, yaitu Perlakuan A : 0,5 ml sperma ditambah air kelapa muda 75% + gliserol 25%, Perlakuan B : 0,5 ml sperma ditambah air kelapa muda 50% + gliserol 50%, Perlakuan C : 0,5 ml sperma ditambah air kelapa muda 25% + gliserol 75%. Hasil penelitian menunjukkan bahwa penambahan air kelapa dan gliserol pada penyimpanan sperma berpengaruh nyata terhadap fertilitas spermatozoa ikan mas dan spermatozoa masih bertahan hidup selama penyimpanan 4 hari, namun semakin lama disimpan motilitas akan semakin menurun. Nilai fertilitas pada hari ke- 0 tidak berpengaruh, namun berpengaruh setelah dilakukan penyimpanan. Hari ke- 1 nilai fertilitas tertinggi pada perlakuan B 53,1%. Hari ke- 2 perlakuan B memiliki nilai tertinggi sebesar 39,3%. Hari ke- 3 perlakuan B didapatkan nilai tertinggi sebesar 15,9%. Hari ke- 4 fertilitas tertinggi dihasilkan pada perlakuan B, yaitu 6,5%. Berdasarkan hasil penelitian dapat disimpulkan bahwa penambahan air kelapa dan gliserol pada penyimpanan sperma berpengaruh nyata terhadap fertilitas spermatozoa ikan mas dan spermatozoa masih bertahan hidup selama penyimpanan 4 hari, namun semakin lama disimpan motilitas akan semakin menurun, serta perlakuan yang terbaik adalah perlakuan B dengan dosis air kelapa muda 50% dan gliserol 50%. ABSTRACT This study aimed to determine the effect of the addition of coconut water and glycerol on fertility carp (Cyprinus carpio L.) spermatozoa and motility conditions as well as the best treatment on sperm storage carp strains Rajadanu. This experiment used Completely Randomized Design (CRD) with 3 treatments and 3 replications, namely Treatment A: 0.5 ml sperm plus coconut water 75% + glycerol 25%, Treatment B: 0.5 ml sperm plus coconut water 50 % + glycerol 50%, Treatment C: 0.5 ml sperm plus coconut water 25% + glycerol 75%. The results showed that the addition of coconut water and glycerol on sperm storage significantly affect sperm fertility carp and surviving spermatozoa during 4 days of storage, but the longer it is stored motility decrease. Fertility values ​​at day-0 has no effect, but the effect after storage. Day-1 value of the highest fertility treatment B 53.1%. Day-2 treatment B had the highest score of 39.3%. Day-3 treatment B earned the highest score of 15.9%. Day-4 produced the highest fertility treatment B, which is 6.5%. Based on the results of this study concluded that the addition of coconut water and glycerol on sperm storage significantly affect fertility carp spermatozoa and spermatozoa were still alive during 4 days of storage, but the longer it is stored motility will decrease, and the best treatment is the treatment of B at a dose of coconut water 50% and glycerol 50%.
PENGGUNAAN EKSTRAK KULIT BUAH MANGGIS (Garcinia mangostana) SEBAGAI ANTIBAKTERI UNTUK MENGOBATI INFEKSI Aeromonas hydrophila PADA IKAN NILA (Oreochromis niloticus) Maisyaroh, Latifah Apriliana; Susilowati, Titik; Haditomo, Alfabetian Herjuno Condro; Yuniarti, Tristiana; Basuki, Fajar
Sains Akuakultur Tropis Vol 2, No 2 (2018)
Publisher : Departemen Akuakultur FPIK UNDIP

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Abstract

Ikan nila (O. niloticus) merupakan salah satu ikan air tawar ekonomis penting. Salah satu kendala utama dalam kegiatan budidaya adalah adanya penyakit, diantaranya Motile Aeromonas Septicema (MAS) yang disebabkan oleh Aeromonas hydrophila. Sekarang ini, penanganan penyakit lebih diutamakan menggunakan obat herbal. Ekstrak kulit buah manggis diduga mengandung senyawa antibakteri yang mungkin dapat digunakan untuk meningkatkan kelulushidupan ikan Nila. Tujuan penelitian ini adalah untuk mengetahui pengaruh perendaman ekstrak kulit buah manggis terhadap kelulushidupan ikan nila yang diinfeksi A. hydrophila. Sebanyak 120 ekor ikan Nila dengan panjang rata-rata 8,55±0,50 cm digunakan pada penelitian ini. Ikan diinfeksi bakteri A. hydrophila sebanyak 0,1 mL secara intramuscular dengan kepadatan 108 CFU/mL. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 pengulangan yaitu perlakuan A (0 mg/L, B (500 mg/L), C (600 mg/L) dan D (700 mg/L). Setelah munculnya gejala klinis, ikan dilakukan perlakuan perendaman dengan ekstrak kulit manggis selama 4 jam. Hasil penelitian ini menunjukkan bahwa perendaman ekstrak kulit buah manggis berpengaruh nyata (P<0,05) terhadap kelulushidupan ikan nila. Perlakuan terbaik ditunjukan pada perlakuan D (700 mg/L) dengan prosentasi kelulushidupan sebesar 76,67±15,28.
A Study on The Domestication of Tiger Snail (Babylonia spirata l) in An Abanont Brackish Water Pond at Different Stocking Denstities Rejeki, Sri; Susilowati, Titik
SAINTEK PERIKANAN Vol 6, No 2 (2011): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

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Abstract

Tiger snail (Babylonia spirata L) is one of molllucs family which has a good economic value both for local and international market. This study was aimed to find out the effects of different stocking densities on the specific growth rate and survival rate of tiger snail cultured in an abandon brackish water pond. A completely randomized design was applied in this research. The treatments were A (18 tiger snail/0,15m2), B (22 tiger snail/0,15m2), C (26 tiger snail/0,15m2), D (30 tiger snail/0,15m2), E (34 tiger snail/0,15m2).  Each treatment was repicated three times.  Plastic baskets at the size of 0.15 were used as a cage for holding system. Tiger snail at initial average body weight of  8 gram approximately was used as tested animal. The study was carried out at an abandon brackish water pond Desa Tapak Kecamatan Semarang Barat, Central java Province from May – July 2007. The results show that different stocking densities affected the specific growth rate and food conversion ratio of tiger snail significantly.  Stocking density of 18 snail/0.15 m2 gave the highest specific growth rate (2.07%/day) and food conversion ratio of 1,747. Keyword: Domestication, tiger snail, stocking density, abandon brackish water pond
The Influence of Depth of Plantation to the Growth Rate of Eucheuma cottonii Seaweed Cultivated by Longline Method in Mlonggo Beach, Jepara Regency Susilowati, Titik; Rejeki, Sri; Zulfitriani, Zulfitriani; Dewi, Eko Nurcahya
SAINTEK PERIKANAN Vol 8, No 1 (2012): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

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Abstract

The aim of the research was to evaluate the influence of three different depth of plantation of Eucheuma cottonii seaweed to the relative and daily growth rate of seaweed.   The experiment   was  designed using a Completely Randomized Design with 3 treatments (25, 50 and 75 cm) depths of Eucheuma  cottonii seaweed. Each treatments was done in triplicate.   Data of the relative and daily growth rate of seaweed are expressed as mean ± standard deviation and then  were analysed using one way Analysis of Variance (ANOVA). A Duncan Multiple test was applied to find out the difference among the treatments. Based on the results, the different depth of plantation gave a highly significant influence (p&lt;0.01) to the the relative and daily growth rate of seaweed. The treatment for 25 cm of the depth plantation showed the relative growth of seaweed of 910% , nearly 3 times of 75cm of depth plantation. The result of this research indicated that the 25 cm depth of plantation was the best relative and daily growth rate of seaweed of all treatments.   Key words : The depth of plantation , relative growth rate, daily growth rate, Eucheuma cottoniii
Different Percentages of Gonad Maturity on the Fertility and Haching Rate in Artificial Breeding of Abalone (Haliotis Asinina) Suminto, Suminto; Sani, Dyah Anggun Permana; Susilowati, Titik
SAINTEK PERIKANAN Vol 5, No 2 (2010): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

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Abstract

Abalone is one of the marine mollusk that has high economic value. Mass abalone cultivation requires the mature gonads broodstock to produce seeds, but the maturity of the gonads of male and female often can not be simultaneously. Artificial insemination using a solution of ammonia is one solution to produce abalone seed in the hatchery. Ammonia solution can increase motility, fertilization rate of eggs and abalone seed production, so the provision of seeds for cultivation of abalone is independent from nature. The research of artificial insemination was carried out at various levels of gonadal maturation dams abalone (H. asinina) to determine the percentage of fertility and hatching rate of eggs from each Gonad Maturity Level. In stadia gonadal recovery, no fertilization occurs, whereas in the gonads maturing stadia had fertilization and hatching rate 24.33 ± 2.08% and 22.12 ± 2.18%, respectively at ripe stadia, gonads had been fertilization and hatching rate were 94.67% ± 1.53% and 82.82% ± 3.58%, wasn’t recorded in the stadia spent fertilization did not occur because the eggs were released in culture media. Gonad Maturity Level, thus can be used in artificial insemination was the ripe gonadal stadia, which had the highest percentage of both levels of fertilization and hatching rate with a diameter ranging from 189.6-252.8 μm. Key Words: Abalone (H. asinine); Artificial breeding; Gonad Maturity Level; Hatching Rate
The Improvement of the Survival, Growth and Production of Vaname Shrimp (Litopenaeus vannamei) and Seaweed (Gracilaria verucosa) based on Polyculture Cultivation Susilowati, Titik; Hutabarat, Johanes; Anggoro, Sutrisno; Zainuri, Muhammad
International Journal of Marine and Aquatic Resource Conservation and Co-existence 2014: IJMARCC Volume 1 Issue 1 Year 2014
Publisher : International Journal of Marine and Aquatic Resource Conservation and Co-existence

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Abstract

The presence of aeration on the cultivation of polyculture system can eliminate oxygen depletion at night while the intensity of light would effect the seaweed Gracilaria verucosa to perform photosynthesis. The supplies of oxygen is to eliminate the oxygen depletion at night until morning, and propose the efficiency of feeding activity, and also stabilize the water quality. The purpose of this research is to improve the survival, growth and production of vaname shrimp, and Gracilaria verucosa. This research was conducted in Jepara Brackishwater Aquaculture Research and Development Center (BBPBAP), from May until August 2013. A number of 75 shrimps and 1,750 g Gracilaria vericosa were cultivated in the 800 L tank, which observed for 96 days. There are four type of treatments, oxygen supply O1 (3,500 lux light intensity), O2 (Aeration with 700 mL minute-1 speed), O3 (light intensity and aeration), O4 (without light and aeration), three replications. This research was conducted using an experimental Randomized Block Design. The survival rate and growth of shrimp (Litopenaeus vannamei) showed a highly significant (p &lt; 0.01) different between the treatments, while the growth and biomass production of seaweed (Gracilaria verucosa) showed a significantly (p &lt; 0.05) different. The three treatments of oxygen and light supply, aeration and the combination of light and aeration, it can be concluded that survival, growth and production of vaname shrimp and Gracilaria verucosa. The combination of light and aeration treatment is the best, it can be concluded survival rate of shrimp (94.64%) compare to the treatment without oxygen (45.24%). Absolute growth rate of shrimp increased from 9.57 g to 12.97 g. The specific growth rate of shrimp increased from 4.73% to 5.07%. The biomass production of shrimp increased from 181.56 g m-2 to 883.95 g m-2. The combination of light and aeration can improve the absolute growth rate of Gracilaria verucosa from 25.86 g to 52.724 g. The specific growth rate of seaweed increased from 1.77% to 2.64% then biomass production of Gracilaria verucosa also to increase from 2,557.76 g to 5,063.2 g.   Key words: Litopenaeus vannamei, Gracilaria verucosa, oxygen supply
PENGARUH BOBOT AWAL YANG BERBEDA TERHADAP PERTUMBUHAN Caulerpa lentillifera YANG DIBUDIDAYAKAN DENGAN METODE Longline DI TAMBAK BANDENGAN, JEPARA Iskandar, Sarah Nur; Rejeki, Sri; Susilowati, Titik
Journal of Aquaculture Management and Technology Volume 4, Nomor 4, Tahun 2015
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Caulerpa lentillifera termasuk kedalam alga hijau. Produksi C. lentillifera masih rendah karena mengandalkan hasil dari alam sehingga dibutukan teknologi budidaya untuk menunjang kontinuitas produksi C. lentillifera. Perbedaan biomassa awal sangat berpengaruh terhadap pertumbuhan rumput laut. Hal ini sangat berkaitan dengan persaingan setiap individu rumput laut dalam mendapatkan unsur hara sebagai makanannya. Keberhasilan sistem penanaman dipengaruhi oleh penggunaan bibit yang baik dan bobot yang sesuai akan meningkatkan pertumbuhan. Penelitian ini bertujuan untuk mengetahui pengaruh perbedaan bobot awal penanaman terhadap pertumbuhan C. lentillifera yang dibudidayakan dengan metode longline dan bobot awal penanaman rumput laut yang memberikan pertumbuhan terbaik. Penelitian ini dilaksanakan pada tanggal 7 Februari 2015 - 22 April 2015 di Tambak Bandengan, Jepara, Jawa Tengah. Tanaman uji yang digunakan dalam penelitian ini adalah rumput laut dari jenis C. lentillifera yang berasal dari Balai Perikanan Budidaya Air Payau (BPBAP) Takalar, Sulawesi. Tanaman uji dibudidayakan dengan metode longline dan dipelihara selama 35 hari. Rancangan yang digunakan adalah rancangan acak lengkap (RAL) dengan 4 perlakuan dan 5 ulangan yaitu perlakuan A (50 g), B (75 g), C (100 g), dan D (125 g). Variabel yang diamati adalah pertumbuhan relatif, laju pertumbuhan spesifik, dan kualitas air. Hasil analisa ragam anova menunjukkan bahwa perbedaan bobot awal berpengaruh sangat nyata (P&lt;0.01) terhadap pertumbuhan C. lentillifera. Hasil uji wilayah ganda (Duncan) menunjukkan perlakuan A dengan bobot 50 g memberikan pertumbuhan terbaik. Kesimpulan yang diperoleh adalah pertumbuhan rumput laut C. lentillifera dengan bobot awal penanaman 50 g memberikan pertumbuhan relatif terbaik sebesar 152.00±10.95%, dan pertumbuhan spesifik terbaik yaitu sebesar 2.64±0.13%/hari dan disarankan untuk dibudidayakan. Caulerpa lentillifera is belong to green algae species. C. lentillifera production is remains low and still depend on natural harvest, in other to cultivation technology is necessary to support the sustainable production of C. lentillifera. Initial weigh of biomass greatly affect the growth of seaweed. It is strongly associated with each individual seaweed competition in getting nutrients as food. The success of the culture system is affected by using a good seed and the appropriate weights will increase the growth. The aims of this study was to determine the effect of different initial weights on the growth of seaweed C. lentillifera and to find out the initial weight that gives the best growth during culture period with Long line method. This study was conducted from February 7th to April 22th 2015. The plants test used in this study was seaweed C. lentillifera that comes from Brackish Water Aquaculture Center (BPBAP) Takalar, Sulawesi. The tested of C. lentillifera cultivated by long line method and maintained for 35 days. This study using experimental design by Completely Randomized Design with 4 treatments and 5 replications. The treatments tested were A (50 g), B (75 g), C (100 g) and D (125 g). The variables measured were relative growth, specific growth rate, and water quality. ANOVA variance analysis results indicate that the initial weight difference was highly significant (P&lt;0.01) on the growth of C. lentillifera, the result of the double region (Duncan) showed treatment A with 50 g in weight gave the best growth. The conclusion of this study that the growth of C. lentillifera with 50 g Initial weigh of planting gives the best result in relative growth rate about 152.00±10.95% and specific growth rate about 2.64±0.13%/day and it recommended to be cultured.
PENGARUH DOSIS PUPUK DAN SUBSTRAT YANG BERBEDA TERHADAP PERTUMBUHAN Caulerpa lentillifera Dahlia, Iis; Rejeki, Sri; Susilowati, Titik
Journal of Aquaculture Management and Technology Volume 4, Nomor 4, Tahun 2015
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Produksi rumput laut  jenis Caulerpa tergolong masih  rendah, sebab sampai saat ini produksi Caulerpa masih mengandalkan hasil dari alam sehingga bergantung pada musim. Penelitian ini bertujuan untuk mengetahui pengaruh dan interaksi dosis pupuk dengan substrat dasar yang berbeda terhadap pertumbuhan C. lentillifera serta untuk mengetahui dosis pupuk dan substrat yang dapat memberikan pertumbuhan terbaik bagi C. lentillifera. Metode penelitian yang digunakan adalah eksperimen, menggunakan analisa Faktorial dengan Rancangan Acak Lengkap (RAL). Faktor yang digunakan terdiri dari faktor A: dosis pupuk dalam media perendaman terdiri dari 4 taraf: A1: 0 ml, A2: 1,5 ml, A3: 2,5 ml dan A4: 3,5 ml, dan faktor B (substrat dasar) terdiri dari 2 taraf: B1: lumpur  berpasir dan B2: pecahan karang mati. Sehingga didapat 8 kombinasi perlakuan, masing-masing diulang sebanyak 3 kali. Berat awal rumput laut yang digunakan adalah 25±0,12 g. Data yang dikumpulkan adalah laju pertumbuhan spesifik dan parameter kualitas air. Data laju pertumbuhan spesifik dianalisis menggunakan dua taraf ANOVA dan dilanjutkan dengan uji Duncan. Hasil ANOVA menunjukkan bahwa dosis pupuk dan substrat dasar yang berbeda memberikan pengaruh dan interaksi yang sangat nyata (Fhitung &gt; Ftabel (5% dan 1%)) terhadap laju pertumbuhan spesifik C. lentillifera. Perlakuan A1B1 (0 ml, lumpur berpasir) memberikan pertumbuhan terbaik dibandingkan perlakuan yang lain, dengan laju pertumbuhan spesifik = 3,65±0,17 %/hari. Parameter kualitas air yang terukur selama pemeliharaan berada dalam kisaran yang sesuai untuk kehidupan C. lentillifera. Caulerpa seaweed production is still low, because until now the production of Caulerpa still relies on the natural harvest of that depends on the season. This study aims to determine the effect and interaction of fertilizers and different bottom substrates on the growth of C. lentillifera as well as to determine the dosage of fertilizers and substrates that can provide the best growth of C. lentillifera. This research was done experimentally, using a factorial analysis with a completely randomized design. Factors used consisted of factors: A: dose of fertilizer WIR 4 levels: A1: 0 ml, A2: 1.5 ml, A3: 2.5 ml and A4: 3.5 ml, and factor B (substrate) consisted of two levels: B1: sandy mud and B2: fragmented of dead coral. Therefore there were 8 treatment combinations, each was repeated 3 times. Initial weight of seaweed used was 25±0.12 g. The data collected were specific growth rate and water quality parameters. The data of specific growth rate was analyzed using two levels ANOVA and followed by Duncan’s test. The ANOVA result shows that different dosage and different bottom substrates highly significantly by (Fcount &gt; Ftable (5% and 1%)) affected and interaction the specific growth rate of C. lentillifera. Treatment A1B1 (0 ml, sandy mud) gives the best result compared with another treatments, with specific growth rate = 3.65±0.17 %/day. The Water quality parameters were still in the proper range for C.lentillifera life.
PENGARUH BOBOT AWAL YANG BERBEDA TERHADAP PERTUMBUHAN RUMPUT LAUT LATOH (Caulerpa lentillifera) YANG DIBUDIDAYA DI DASAR TAMBAK, JEPARA Novianti, Denisa Novianti; Rejeki, Sri; Susilowati, Titik
Journal of Aquaculture Management and Technology Volume 4, Nomor 4, Tahun 2015
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Caulerpa lentillifera merupakan kelompok alga hijau yang memiliki kandungan antioksidan tinggi. Ketersediaan C. lentillifera masih bergantung pada alam dan belum dibudidayakan secara baik. Penelitian ini bertujuan untuk mengetahui pengaruh bobot awal yang berbeda terhadap pertumbuhan rumput laut C. lentillifera dan mengetahui bobot awal yang memberikan pertumbuhan terbaik yang di budidayakan dengan metode dasar. Penelitian ini dilaksanakan pada bulan Maret-April 2015. Penelitian ini menggunakan rancangan percobaan dengan menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 5 ulangan. Perlakuan yang diujikan adalah perlakuan A (50 g), B (75 g), C (100 g) dan D (125 g). Rumput laut dipelihara didalam keranjang dan direndam di dasar tambak. Hasil penelitian menunjukan bahwa perbedaan bobot pada awal tanam berpengaruh sangat nyata terhadap pertumbuhan C. lentillifera dan hasil Specific Growth Rate (SGR) terbaik ditunjukan oleh perlakuan A (50 g) yaitu, 2.66±0.10 %/hari.Caulerpa lentillifera is one of green algae that have high antioxidant content. However, production of C. lentillifera is still depend on natural harvest. The aims of this study was to determine the effect of different initial weights on the growth of seaweed C. lentillifera and to find out the initial weight that gives the best growth during culture period with bottom methods. This study was conducted from March to April 2015 using experimental design by Completely Randomized Design with 4 treatments and 5 replications. The treatments tested were A (50 g), B (75 g), C (100 g) and D (125 g). Seaweed placed in a basket and immersed in to the pond bottom. The results showed that different innitial weight significantly affected the growth of the C. lentillifera and the best result of Relative growt rate was shown by treatment A (50 g) with average growth 2.66±0.10%/day.
PENGARUH KEPADATAN BERBEDA MENGGUNAKAN rGH PADA PAKAN TERHADAP PERTUMBUHAN DAN KELANGSUNGAN HIDUP IKAN NILA (Oreochromis niloticus) Sibarani, Dian Afdelima; Susilowati, Titik; Yuniarti, Tristiana
Journal of Aquaculture Management and Technology Volume 4, Nomor 3, Tahun 2015
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Kepadatan merupakan hal yang penting dalam usaha pendederan karena akan mempengaruhi oksigen terlarut dan ammonia. Pemberian rGH dengan metode oral atau melalui pakan terbukti dapat mempercepat pertumbuhan ikan dikarenakan rGH yang tercampur dalam pakan dapat lebih mudah masuk kedalam tubuh ikan. Fungsi dari rGH adalah sebagai pengatur pertumbuhan, reproduksi, system imun, tekanan osmosis pada ikan teleostei, dan  pengatur metabolism. Pemanfaatan sistem resirkulasi dapat menciptakan lingkungan yang optimal bagi pertumbuhan ikan. Hal tersebut dapat menghasilkan tingkat produktivitas yang tinggi dalam wadah budidaya dengan mortalitas yang rendah dan tingkat kelulushidupan yang tinggi. Sistem resirkulasi merupakan wadah pemeliharaan ikan yang menggunakan system perputaran air yaitu air mengalir dari satu wadah ke wadah yang lain melalui filter yang berguna untuk menjaga kualias air. Penelitian ini dilakukan dengan tujuan untuk mengetahui pengaruh kepadatan yang berbeda terhadap pertumbuhan dan kelangsungan hidup ikan nila yang menggunakan rGH dengan kepadatan berbeda pada sistem resirkulasi dan untuk mengetahui kepadatan yang dapat memberikan pertumbuhan dan kelulushidupan terbaik. Penelitian ini dilakukan di Balai Benih Ikan Siwarak, Ungaran, Kab. Semarang. Penelitian dilakukan selama 63 hari dari bulan November 2014 – Januari 2015. Wadah yang digunakan berupa akuarium ukuran (50x30x30) cm3 sebanyak 12 buah yang diisi air sebanyak 20 liter dan dialiri air dari ember yang sudah diisi dengan filter bioball. Rancangan yang digunakan adalah rancangan acak lengkap dengan padat penebaran 20, 40, 60 dan 80 ekor/wadah, dimana masing-masing perlakuan dilakukan pengulangan sebanyak tiga kali. Jenis pakan bubuk berupa pakan komersial diberikan secara at satiation. Kesimpulan yang dapat diambil dari penelitian ini adalah padat penebaran tidak berbeda nyata terhadap kelulushidupan, namun memberikan perbedaan yang nyata terhadap pertumbuhan panjang mutlak dan perbedaan yang sangat nyata terhadap laju pertumbuhan spesifik. Kepadatan yang dapat memberikan pertumbuhan dan kelulushidupan terbaik terdapat pada padat penebaran sebanyak 20 ekor/wadah, dimana dengan nilai Pertumbuhan Panjang Mutlak adalah 9,08±0,43, nilai SGR 9,07 %/hari, nilai FCR 1,18±0,00, dan PER 3.33±0.10, nilai kelulushidupan terbaik adalah perlakuan A (padat penebaran 20 ekor/wadah) yang sama besarnya dengan perlakuan B (padat penebaran 40 ekor/wadah) yaitu sebesar 96,67 %. Density is important factor in breeding method because it will affected to dissolved oxygen and ammonia level. Giving rGH with oral method or adding into feed  has been proven to increasing the growth of Tilapia because rGH which adding into feed can enter to fish body easily. Function of rGH as growth, reproduction, immune system, osmotic pressure in teleost fishes and metabolism system controlling. Using recirculating system can creating an optimal environment for fish’s growth. If that will happen, can produce a high productivity in culture pond with low mortality and high survival rate. Reciculating system is fish culture tank that using water circulating then flowing tank to tank through a filter which function is maintaining of water quality. This research was aimed to find out the effect of different rearing density that using  rGH to growth and survival rate of Tilapia larvae in recirculating system and knows the best density for increasing growth and survival rate. This research was conducted in 63 days from November 2014 to January  2015 at Balai Benih Ikan Siwarak, Ungaran, Kab. Semarang. The fish culture tank is an aquarium size of 50x30x30cm, total amount 12, each aquarium filled water 20 liters and flowing water from bucket that filled with bioball filter. This research used Completely Randomised Design with five treatment (stock density 20, 40, 60 and 80 fish/tank) and three replication. Feed type is a powder commercial feed with using feeding method at satiation. The results is different rearing density not significantly different for survival rate, but significantly different for relative growth rate (RGR) and very significantly different for Specific Growth Rate (SGR). Stocking density that giving the best growth and survival rate is 20 fish/tank (treatment A) with RGR 9,08 ± 0,43, SGR 9,07%/day, FCR is 1,18±0,00 and PER 3,33±0,10, the best survival rate in treatment A (20 fish/tank) and treatment B (40 fish/tank) is 96,67%.