Hani Susianti
Laboratorium Patologi Klinik Rumah Sakit Umum Dr. Saiful Anwar Malang

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Kadar Antibodi Anti-dsDNA dan Urine Monocyte Chemoattractant Protein-1 pada Nefritis Lupus Susianti, Hani; Salman, Yuliana; Gunawan, Atma; Handono, Kusworini
Jurnal Kedokteran Brawijaya Vol 27, No 2 (2012)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Abstract

ABSTRAKKata Kunci: Lupus  eritematosus  sistemik  (LES) merupakan  penyakit  autoimun  yang  ditandai  oleh  peradangan  kronis  dan  akut. Biomarker klasik untuk mendeteksi adanya penyakit LES adalah antibody anti-double stranded DNA (anti-dsDNA) dan urine Monocyte Chemoattractant Protein-1   (uMCP-1).   Penelitian ini dilakukan untuk mengkaji hubungan antara kedua biomarker tersebut dengan klasifikasi histopatologis nefritis lupus untuk mengganti biopsi ginjal dalam penentuan kelas histopatologi nefritis lupus.   Penelitian ini dilakukan selama 11 bulan   berupa studi observasional dengan pengambilan sampel darah dan urin untuk mengetahui kadar antibodi anti-dsDNA dan MCP-1, serta biopsi ginjal untuk menentukan kelas nefritis lupus berdasarkan klasifikasi WHO tahun 1982.   Data hasil penelitian menunjukkan tidak ada perbedaan yang signifikan (p=0,208>α) antara mean rank kadar anti-dsDNA pada kelompok kontrol dan kelompok kasus,begitu pula dengan hasil perbandingan mean rank kadar uMCP-1 (p=0,247>α).   Uji korelasi Spearmans rho,menunjukkan hubungan signifikan kadar anti-dsDNA dan kadar uMCP-1 (r = 0,861; p<0,001). Dapat disimpulkan bahwa tidak terdapat perbedaan kadar biomarker antibodi anti-dsDNA dan urine MCP1 pada kejadian nefritis lupus dan klasifikasi histopatologi nefritis lupus, namun terdapat hubungan yang sangat erat antara kadar biomarker antibodi anti-dsDNA dengan kadar urine MCP-1. Nilai sensitifitas kadar anti-dsDNA dan uMCP-1  lebih rendah yaitu 20% – 40% dibandingkan dengan nilai spesifisitasnya, yaitu 50% – 83,33%.Kata Kunci: Anti-dsDNA, uMCP-1, klasifikasi histopatologi, nefritis  lupus
Kadar Antibodi Anti-dsDNA dan Urine Monocyte Chemoattractant Protein-1 pada Nefritis Lupus Susianti, Hani; Salman, Yuliana; Gunawan, Atma; Handono, Kusworini
Jurnal Kedokteran Brawijaya Vol 27, No 2 (2012)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Original Source | Check in Google Scholar

Abstract

ABSTRAKKata Kunci: Lupus  eritematosus  sistemik  (LES) merupakan  penyakit  autoimun  yang  ditandai  oleh  peradangan  kronis  dan  akut. Biomarker klasik untuk mendeteksi adanya penyakit LES adalah antibody anti-double stranded DNA (anti-dsDNA) dan urine Monocyte Chemoattractant Protein-1   (uMCP-1).   Penelitian ini dilakukan untuk mengkaji hubungan antara kedua biomarker tersebut dengan klasifikasi histopatologis nefritis lupus untuk mengganti biopsi ginjal dalam penentuan kelas histopatologi nefritis lupus.   Penelitian ini dilakukan selama 11 bulan   berupa studi observasional dengan pengambilan sampel darah dan urin untuk mengetahui kadar antibodi anti-dsDNA dan MCP-1, serta biopsi ginjal untuk menentukan kelas nefritis lupus berdasarkan klasifikasi WHO tahun 1982.   Data hasil penelitian menunjukkan tidak ada perbedaan yang signifikan (p=0,208>α) antara mean rank kadar anti-dsDNA pada kelompok kontrol dan kelompok kasus,begitu pula dengan hasil perbandingan mean rank kadar uMCP-1 (p=0,247>α).   Uji korelasi Spearmans rho,menunjukkan hubungan signifikan kadar anti-dsDNA dan kadar uMCP-1 (r = 0,861; p<0,001). Dapat disimpulkan bahwa tidak terdapat perbedaan kadar biomarker antibodi anti-dsDNA dan urine MCP1 pada kejadian nefritis lupus dan klasifikasi histopatologi nefritis lupus, namun terdapat hubungan yang sangat erat antara kadar biomarker antibodi anti-dsDNA dengan kadar urine MCP-1. Nilai sensitifitas kadar anti-dsDNA dan uMCP-1  lebih rendah yaitu 20% – 40% dibandingkan dengan nilai spesifisitasnya, yaitu 50% – 83,33%.Kata Kunci: Anti-dsDNA, uMCP-1, klasifikasi histopatologi, nefritis  lupus
Proteinuria Severity in Lupus Nephritis is Associated with Anti-dsDNA Level and Immune Complex Deposit Location in Kidney Engli, Katherina; Handono, Kusworini; Eko, Mudjiwijono Handaru; Susianti, Hani; Gunawan, Atma; Kalim, Handono
Journal of Tropical Life Science Vol 8, No 3 (2018)
Publisher : Journal of Tropical Life Science

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Abstract

Lupus nephritis (LN) is one of the manifestations of Systemic Lupus Erythematosus (SLE), with proteinuria being one of the clinical manifestations. The proteinuria pathogenesis is associated with anti-dsDNA antibody and the location of immune complex deposits within the kidney. This study aims to investigate the correlation of the severity of proteinuria with the location of immune complex deposits and the level of anti-dsDNA antibody in LN. Data were collected in cross-section. Fifty-three patients with LN in Saiful Anwar Hospital Malang, who underwent renal biopsy, were included. Hematoxylin-eosin staining and immunofluorescence analysis were used to assign subjects to different histopathological classes and determine the immune complex deposits. The spot urine samples were evaluated using the dipstick method for semi-quantitative proteinuria. The anti-dsDNA antibody levels were evaluated using the enzyme-linked immunosorbent assay (ELISA). Turbidity and enzymatic tests were conducted to elucidate urine protein and creatinine content, respectively. The level of proteinuria is significantly different among the different locations of immune complex based on the dipstick and protein/creatinine methods (p = 0.021 and p = 0.005, respectively). There was a significant correlation between anti-dsDNA antibody level and the severity of proteinuria (r = 0.326 based on dipstick and r = 0.28 based on protein/creatinine method). Thus, proteinuria in LN is determined by anti-dsDNA level and the location of immune complex deposits in the kidney.
Urine Specific Proteins and Alpha-1 Antitrypsin Concentrations to Assess the Severity of Lupus Nephritis Susianti, Hani; Barlianto, Wisnu; Hanggara, Dian Sukma; Handono, Kusworini; Adipireno, Purwanto; Suromo, Lisyani
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

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Abstract

Background. Current biomarkers for evaluating disease activity or severity in lupus nephritis (LN) are considered to be unsatisfactory. Pathological changes in glomerular basement membrane and selectivity of electrical discharge are causing specific patterns of urine proteins excretion. Together with alpha-1 antitrypsin (AAT), they are expected to become new biomarkers to assess LN activity.Method. Seventy-one urine samples were collected from healthy controls and LN patients. Patterns of urine specific proteins were determined using column chromatography and SDS-PAGE tests, LN activity was calculated using SLEDAI-renal domain score, and AAT concentrations was measured by ELISA.Result. The majority of proteins in the control group have molecular weights of >66 kDa (88%) and 21- to 25-kDa proteins were observed only in the case group. The p values for differences in urine AAT concentration between active LN and healthy controls, inactive LN and healthy controls, and active LN and inactive LN were 0.004, 0.046, and 0.054, respectively, whereas those for urine AAT/creatinine ratio were 0.489, 0.019, and 0.915, respectively.Conclusion: There were differences in the patterns of the molecular weight of proteins and urine AAT concentrations between case group and control group. However, no such differences were identified between active and inactive LN. 
Development of Candidate Antigens for Rapid Test Kit to Detect Autoantibodies in Patients with Systemic Lupus Erythematosus Barlianto, Wisnu; Susianti, Hani; Wahono, Singgih; Ismayasih, Nelly; Meilani, Rossy; Handono, Kusworini
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

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Abstract

Systemic Lupus Erythematosus (LES) is an autoimmune inflammatory disease characterized by the formation of anti-nuclear antibodies (ANA) and anti-double stranded DNA (anti-dsDNA) antibodies as diagnostic markers. Detection of such autoantibodies requires advanced equipment and trained personnel.This study was conducted to acquire candidate antigens that can be used for rapid test kit for practical and accurate detection of ANA and anti-dsDNA to speed up SLE diagnosis.Nuclear proteins and DNA derived from cell lines, hair follicles, and leukocytes of SLE patients and healthy individuals were isolated using QiaGEN kit and modified-manual procedure. Antigen-antibody bonds were tested by dot blot assay.The strongest binding between DNA antigens of a healthy individual and antibodies occurred at dilution factors of 1:5,120 for the antigen and 1:2,560 for the antibody. The strongest binding between nuclear protein antigens from the cell line and antibodies occurred at dilution factors of 1:512 for the antigen and 1:1,600 for the antibody.Nuclear antigens derived from cell line and DNA antigens of healthy individuals were antigen candidates for the development of ANA and anti-dsDNA rapid detection tests.
Differences Between Alpha-Fetoprotein (AFP) and Prothrombine Induced by Vitamin K Absence or Antagonist II (PIVKA-II) Values as Early Detection Method for Hepatocellular Carcinoma (HCC) and Cirrhosis Nugraha, Bayu Eka; Setiawan, Nugraha; Susianti, Hani; Pratomo, Bogi
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy Vol 19, No 3 (2018): VOLUME 19, NUMBER 3, December 2018
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Abstract

Background: Hepatocellular carcinoma is a common cancer worldwide and has a high mortality. Biomarkers could theoretically help to detect the disease at an earlier stage before symptoms occur and improve the treatment outcomes. The first biomarker found was AFP (not very accurate and 30-40% of HCC may be missed). PIVKA-II can be used as an early detection method to diagnose HCC.Method: A cross sectional study on in-patients or out-patients at Saiful Anwar Malang Hospital from July 2016 to October 2016.Results: The p value (p > 0.05) obtained using Kolmogorov-Smirnov was 0.166 for diagnosis of HCC and 0.147 for the diagnosis of hepatic cirrhosis. The p value (p > 0.05) obtained using Shapiro-Wilk was 0.103 for diagnosis of HCC and 0.087 for the diagnosis of cirrhosis. Comparative test using the LSD method showed PIVKA-II serum levels in HCC as compared to hepatic cirrhosis as significant with a p-value less than 0.05 (p < 0.05), that is 0.025. However comparative test using the Tukey HSD method showed that the results obtained were not significant. According to the PIVKA-II cut off value, the sensitivity and specificity to detect cirrhosis and HCC was as large as 100%. According to the AFP cut off value, the sensitivity to detect cirrhosis and HCC was 93.3% and the specificity was 76.92%.Conclusion: Both PIVKA-II and AFP can be used to detect cirrhosis and HCC. However PIVKA-II exhibited better sensitivity and specificity in the detection of cirrhosis and HCC.
Differences Between Alpha-Fetoprotein (AFP) and Prothrombine Induced by Vitamin K Absence or Antagonist II (PIVKA-II) Values as Early Detection Method for Hepatocellular Carcinoma (HCC) and Cirrhosis Nugraha, Bayu Eka; Setiawan, Nugraha; Susianti, Hani; Pratomo, Bogi
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy Vol 19, No 3 (2018): VOLUME 19, NUMBER 3, December 2018
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Abstract

Background: Hepatocellular carcinoma is a common cancer worldwide and has a high mortality. Biomarkers could theoretically help to detect the disease at an earlier stage before symptoms occur and improve the treatment outcomes. The first biomarker found was AFP (not very accurate and 30-40% of HCC may be missed). PIVKA-II can be used as an early detection method to diagnose HCC.Method: A cross sectional study on in-patients or out-patients at Saiful Anwar Malang Hospital from July 2016 to October 2016.Results: The p value (p > 0.05) obtained using Kolmogorov-Smirnov was 0.166 for diagnosis of HCC and 0.147 for the diagnosis of hepatic cirrhosis. The p value (p > 0.05) obtained using Shapiro-Wilk was 0.103 for diagnosis of HCC and 0.087 for the diagnosis of cirrhosis. Comparative test using the LSD method showed PIVKA-II serum levels in HCC as compared to hepatic cirrhosis as significant with a p-value less than 0.05 (p < 0.05), that is 0.025. However comparative test using the Tukey HSD method showed that the results obtained were not significant. According to the PIVKA-II cut off value, the sensitivity and specificity to detect cirrhosis and HCC was as large as 100%. According to the AFP cut off value, the sensitivity to detect cirrhosis and HCC was 93.3% and the specificity was 76.92%.Conclusion: Both PIVKA-II and AFP can be used to detect cirrhosis and HCC. However PIVKA-II exhibited better sensitivity and specificity in the detection of cirrhosis and HCC.