Metode Lepas Dasar dengan Model Cidaun pada Budidaya Eucheuma spinosum (Linnaeus) Agardh

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 10, No 3 (2005): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Rumput laut tergolong dalam algae sebagai tumbuhan yang tidak dapat dibedakan antara akar, batang dan daun, sehingga seluruh bagian tumbuhan disebut thallus. Tujuan dari penelitian ini adalah untuk mengetahui laju pertumbuhan harian Eucheuma spinosum dengan metode lepas dasar menggunakan model Cidaun di perairan Karimunjawa. Selama penelitian berlangsung diukur kualitas air meliputi salinitas, temperatur, pH, kecerahan, arus, sedimentasi dan kedalaman. Eucheuma spinosum sebagai materi penelitian diambil dari lokasi budidaya rumput laut SMKN Karimunjawa, Jepara dan penelitian ini dilaksanakan di selat Takak Alu-alu, perairan Karimunjawa, Jepara antara bulan Oktober dan November 2003. Model Cidaun adalah teknik budidayarumput laut yang digunakan khusus untuk daerah berombak besar dengan menggunakan jaring kantong untuk melindungan thallusnya. Metode penelitian ini adalah eksperimen dengan Rancangan Acak Lengkap pola faktorial 2 x 2. Perlakuan terdiri dari jarak tanam (2 m dan 1,5 m) dan kedalaman (70 cm dan 100 cm) dengan 5 ulangan. Berdasarkan hasil penelitian, membuktikan bahwa penggunaan jaring kantong sebagai model Cidaun tidak begitu efektif, bila dilakukan pada musim pancaroba (Oktober-Desember) karena ombak relatif tenang. Sehingga memudahkan tumbuhnya lumut dan epifit lainnya yang mengganggu pertumbuhan Eucheuma spinosum. Berdasarkan analisa sidik ragam terdapat beda nyata pada kedalaman dan interaksi antarperlakuan.Kata kunci : Laju pertumbuhan harian, Eucheuma spinosum, model CidaunSeaweed belongs algae which can not distinguishable among root, leaf and stem, all of parts called thallus. This experiment was aimed to know growth rate of Eucheuma spinosum use off bottom method with theCidaun’s model at Karimunjawa waters. The water quality was measured during this experiment such as: salinity, temperature, pH, water brightness, current, sediment, and depth. This experiment used Eucheuma spinosum from the cultivation area of SMKN Karimunjawa, Jepara and was carried out at Takak Alualu bay, Karimunjawa waters, Jepara between Oktober and November 2003. The Cidaun’s model is a technique ofseaweed culture used special for the strong wavy area which is using net bag to protect plants. The experimental design used factorial 2 x 2 based on Completely Randomized Design and five replications. The treatmentswere planting distances ( 2 m and 1.5 m) and deepness factors (70 cm and 100 cm). Based on the result explained that the Cidaun’s model was not effective if it is applied during the transition season (October-December), because the wave was relatively calm. That way it made easy mosses and associated plant and it effected the growth rate of Eucheuma spinosum. The result of statistical analysis showed that there weresignificant differences between deepness and the interaction among treatments.Key words : Daily Growth Rate, Eucheuma Spinosum, Cidaun’s model, net bag, depth and distance.

Produksi Bioetanol Dari Tepung Agar Gracilaria verrucosa (Hudson) Papenfuss yang Dihidrolisis Dengan Menggunakan Larutan Asam Sulfat

Journal of Marine Research Vol 2, No 2 (2013) : Journal of Marine Research
Publisher : Journal of Marine Research

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Fossil fuels are non-renewable fuels and overexploited. It has an impact on depletion oil reserves so needed renewable energy such as bio-ethanol. Bioethanol is alternative fuel made from biomass containing component sugars, starches, and cellulose. Agar is polysaccharide in cell wall seaweed Gracilaria verrucosa. Polysaccharides can be hydrolyzed with chemically or enzymatically into monosaccharides and then be fermented into bioethanol. The purpose of this study was to determine the potential of powder agar for bioethanol feedstock; optimum sulfuric acid to hydrolyze powder agar, and knowing optimum fermentation time on ethanol production. The study was conducted from February to June 2012 in Microbiology Laboratory, Research Center for Biology-LIPI, Cibinong. Powder agar is obtained from manufacturers in Malang. Yeast Saccharomyces cerevisiae is a collection of LIPI-MC. Reducing sugar content was analyzed by the method of Miller (1959), while concentration ethanol were analyzed by Gas Chromatography (GC). Method used in this study is an experimental laboratory for the hydrolysis and repeatable measurements for fermentation processes. Hydrolysis process using concentration H2SO4 of 0M, 0.1 M, 0.3 M, and 0.5 M. Fermentation using yeast S. cerevisiae with the incubation time 120 hours. Results showed powder agar can be used as raw material for bioethanol. Optimum reduction sugar 35.38 mg/ml ± 0.59 obtained in H2SO4 0.3 M. Ethanol Optimum 0.77%, obtained at 120 hours incubation.


Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Biofouling is a settlement process from marine organisms initiated by biofilm (microbial layers). Corrosion on the vessels, pipelines under the sea, oil plants, etc are big problems in the industrial today cause adversely affects. Antifouling paints have been developed to prevent settlement of organisms, however antifouling paints contain heavy metals and biocides which have toxic effects on marine ecosystems. Antifouling compound which environmental friendly is a solution for one of the paint component. The aim of this research was to isolate bacteria associated with brown algae which had potential to produce antifouling compound from Karimunjawa islands, Jepara.Isolate KS1-1 showed antifouling activity against Pseudomonas aeruginosa as biofilm-forming bacteria.This isolate had been characterized in morphology, moleculer and biochemichal identification.Moleculer analyses of 16S rRNA sequence, KS1-1 showed similarity 94% from 287 bp with Vibrio sp. W-137-16S ribosomal RNA gene, partial sequence. Keywords : Antifouling, Phaeophyta,Biofouling, 16S rRNA, Vibrio

BIOLOGI LAUT (Special of Biological Reproduction For Seaweed (Gracilaria)) DAN BEASISWA LUAR NEGERI

Prosiding Seminar Biologi Vol 11, No 1 (2014): Seminar Nasional XI Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Mutation of mtDNA ND1 Gene in 20 Type 2 Diabetes Mellitus Patients of Gorontalonese and Javanese Ethnicity

HAYATI Journal of Biosciences Vol 21, No 4 (2014): Desember 2014
Publisher : Bogor Agricultural University, Indonesia

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Mitochondrial gene mutation plays a role in the development of type two diabetes mellitus (T2DM). A point mutation in the mitochondrial gene Nicotinamide adenine dinucleotide dehydrogenase 1 (mtDNA ND1) gene mainly reported as the most common mutation related to T2DM. However, several studies have identified another SNP (single-nucleotide polymorphisms) in the RNA region of mtDNA from patients from specific ethnic populations in Indonesia. Building on those findings, this study aimed to use PCR and DNA sequencing technology to identify nucleotides in RNA and ND1 fragment from 20 Gorontalonese and 20 Javanese T2DM patients, that may trigger T2DM expression. The results showed successful amplification of RNA along 294 bp for all samples. From these samples, we found two types of point  mutation in Javanese patients in the G3316A and  T3200C points of the rRNA and ND1 gene. In samples taken from Gorontalonese patients, no mutation were found in the RNA or ND1 region. We conclude that T2DM was triggered differently in our two populations. While genetic mutation is implicated for the 20 Javanese patients, T2DM pathogenesis in the Gorontalonese patients must be traced to other genetic, environmental, or behavioral factors.


Jurnal Perikanan Universitas Gadjah Mada Vol 12, No 1 (2010)
Publisher : Universitas Gadjah Mada

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Sargassum sp. is one of seaweed species that abundance and widespread in Indonesia. Besides alginates, Sargassum sp. also contains photosynthesis pigment i.e. chlorophyll a and c, carotene, and fucoxanthin. To date, Sargassum sp. is only used in the form of alginates colloid for industrial raw material with drying process prior to production. Dried process of Sargassum sp. cause pigment degradation especially chlorophyll. The aim of this research was to determine the content and pigment composition of fresh and dried Sargassum sp. Pigment identifi  cation was conducted by Thin Layer Chromatography (TLC), Spectrophotometer and High Performance Liquid Chromatography (HPLC). The contents were analyzed by HPLC. Results showed that Sargassum sp. contain pigment fucoxanthin range from 21.80 to 73.10%, chlorophyll a range from 0.73 to 54.96%, chlorophyll c range from 0.28 to 1.09% and carotene 0.38%. The research results showed that fresh and dry Sargassum sp. contain same pigment composition that were as carotene, pheophytin a, chlorophyll a, chlorophyll c and fucoxanthin.