T. Susanti
Indonesian Research Institute for Animal Production

Published : 2 Documents

Found 2 Documents

Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 3 (2012): (September)
Publisher : Diponegoro University

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The aims of this study were to obtain information on the mechanism of molting and the prolactinhormone levels affecting egg production. The study utilized AP (crossbred of Alabio ♂ with Peking ♀)and PA (crossbred of Peking ♂ and Alabio ♀ ducks) with a total of 180 birds. The observed variableswere the duration of cessation of egg production before and after molting, the prolactin hormone level inthe period of molting, the egg production period before and after molting. The data was analyzed usingANOVA, regression and correlation. The results showed that AP crossbred had fewer molting (23.33%)compared to PA (50.00%). The mechanism of molting is always preceded by cessation of eggproduction, molting and relaying. The prolactin hormone concentrations of AP and PA in the periodbefore and after molting were significantly higher than in the period of molting. At the egg productionperiod before molting, the prolactin hormone concentration of AP ducks was higher than the PA ducks.So that the egg production of AP before molting (0-16 weeks) was higher than the PA. The eggproduction of AP was higher than PA, 256.66±6.00 vs 232.22±6.64 eggs for 48 weeks. So it can beconcluded that the prolactin hormone affects the molting and egg production.
Analysis of Prolactin Gene Exon 4 Diversity in Peking, White Mojosari, and Peking White Mojosari Crossbreed Indriati, M.; Sumantri, C.; Susanti, T.
Media Peternakan Vol 39, No 1 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Genetic marker linked to loci reproductive traits could be used to increase an effectiveness of improvement in animal breeding. Association between DNA polymorphism and a trait could be considered as candidate genetic marker for marker assisted selection (MAS) programs. Prolactin (PRL) is one of polypeptide hormones secreted by anterior pituitary gland in vertebrates. PRL plays an important role in onset of poultry incubation and brooding behavior. The aim of this study was to investigate the diversity of prolactin gene and to characterize the type of mutation in partial intron 3, intron 4 and exon 4 of duck prolactin gene. Blood extraction was collected from 168 ducks consisted of 19 Peking, 36 Mojosari, and 113 Peking White Mojosari (Peking Mojosari putih) ducks. Polymerase chain reaction of fragment prolactin gene exon 4 and partial intron 3 and 4 have been successfully amplified with length of base pair were 496 bp. A total of 30 ?L PCR product from each sample were sequenced for forward sequence using BIOTRACE 3730 by First Base Company, Malaysia. Alignment analysis found six SNP consisted of g.3941T>G, g.3975C>A, g.4110T>C, INDEL 3724A,? INDEL 34031, and INDEL 3939A. Analysis of SNP frequency result indicated mutation of INDEL 3724A, g.3941T>G, g.3975C>A, INDEL 4031A and g.4110T>A in duck sample were polymorphic and INDEL 3939A were monomorphic.