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Detection of aggressive periodontitis by calprotectin expression

Dental Journal (Majalah Kedokteran Gigi) Vol 42, No 4 (2009): (December 2009)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

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Abstract

Background: Calprotectin is a calcium-binding protein expressed by neutrophil, monocytes, gingival keratinocytes, and oral epithelial cells. The concentrations of calprotectin increase in plasma, urine and synovial fluid of patients with inflammatory diseases. This protein is known as a marker for periodontal diseases and is detected in gingival crevicular fluids. Purpose: This study was aimed to investigate the detection of inflammation on the aggressive periodontitis by calprotectin expression. Method: The gingival crevicular fluids were taken from five aggressive periodontitis patients and five healthy subjects by using sterile paper points. Calprotectin expression was analyzed by ELISA technique. Result: The results showed the significant difference in calprotectin expression between subject with aggressive periodontitis and healthy subjects p = 0.002 (p < 0.05). Conclusion: It was concluded that the calprotectin expression on the aggressive periodontitis patients may be useful for evaluation the progression of inflammation in periodontitis.

Calprotectin mRNA (MRP8/MRP14) expression in neutrophils of periodontitis patients with type 2 diabetes mellitus

Dental Journal (Majalah Kedokteran Gigi) Vol 42, No 3 (2009): (September 2009)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

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Abstract

Background: Calprotectin, a major cytosolic protein of leukocytes, is detected in neutrophils and monocytes/machrophages. This protein is known to be a marker for several inflammatory diseases including periodontitis. In type 2 diabetes mellitus patients, the severity of periodontitis was strongly thought to be caused by decreasing of leukocytes function such as neutrophils. Previous research found that the calprotectin level in serum of periodontitis patients with type 2 DM is higher than periodontits patients non DM. Purpose: The aim of this study was to determine calprotectin mRNA (MRP8/MRP14) expression in human neutrophils of periodontitis patients with type 2 diabetes mellitus. Methods: Neutrophils were isolated from the peripheral blood of periodontitis patients with uncontrolled type 2 DM, controlled type 2 DM, and non DM. The expression of calprotectin mRNA (MRP8 and MRP14) were detected by RTPCR. Result: The result showed that the value of mRNA calprotectin expression in DM patients were higher than non DM, and the highest expression was on the uncontrolled type 2 DM. Conclusion: The basal level of calprotectin mRNA MRP8/MRP14 expression increased in neutrophil of periodontitis patient with type 2 DM compared non diabetic subjects. It was suggested that high basal level of calprotectin mRNA has a role in the regulation of periodontitis severity with diabetes mellitus patients.

Co-Authors AB Susanto Achmad Widodo Adi Priyo Wicaksono, Adi Priyo Agung Chandra Prasetya Agung Nugroho Setiawan, Agung Nugroho Agus Indarjo Agus Sulistiyo Agus Suyanto Agus Syafrudin Agus Triyanto Ahmad Syaify, Ahmad Aini Moeljono, Aini AL. Supartinah, AL. Andika Bagus Setiawan, Andika Bagus Andri Wahyu Septo Putra Anifatur Nurjannah, Anifatur Arbiyanti, Yossi Norma Arfian, Finty Ari Anshori, Ari Ari Suwondo Arief Suseno, Arief Arif Syaifuddin Arifin Budi Putro, Arifin Budi Arinianzah, Aldo Rizqi Aris Wijayanti Arista, Desi Arlien Siswanti Arnefia Mei Yusnida Asfan Muqtadir, Asfan Astuti, Anjar Ave Gierdo Alfaseno, Ave Gierdo Baliyan I, Baliyan Bambang Nugraha, Bambang Basuki Basuki Bayu Surarso Budi Setiawan Chrisna A Suryono Chrisna Adhi Suryono Cicilia Artitin Cynthia Damayanti Dadang Sukandar Dayyana, Surya Desi Sandra Sari Desie Dwi Wisudanti, Desie Dwi Dewi Agustina Dewi Sulistyaningrum Dhidik Prastiyanto Diana Layla Dimas Putra Anugrah, Dimas Putra Djoko Adi Widodo Donny Prasetyo Santoso, Donny Prasetyo Drajat Martianto Dwi Puji Adiana, Dwi Puji Edi Wibowo Edi Wibowo Kushartono Edy Junaidi Eka Miftakhul Jannah, Eka Miftakhul Eko Supraptono Emi Agustina, Emi Endah Setiyaningrum Endang Sri Susilo ENNY SUSWATI Ervia Yudiati Evi Setiawati Fatekurohman, Mohamat fatimah Fatimah Febriana Tirta Kirana, Febriana Tirta Fitri Mahardika Sari Frida Fallo Gatot Murti Wibowo, Gatot Murti Gatot Yulianto Gentur Handoyo Gigih Budhiawan Gunawan Widi Santosa Hadi Endrawati Harindra W Pradhana, Harindra W Harindra Wisnu Pradhana Haryati Haryati Hayat Maulana Heri Sugito Heri Sutanto Hernita Kusuma Wardani Hery Sulistyo JNS, Hery Sulistyo Hery Widijanto Hidayat, Syarief Thaufik Hilmi Trian Setyawan Indhana Sudiharto, Indhana Indri Trifiantari Irianiwati Irianiwati Irwani Irwani Isa Nurnusanto, Isa Isnaini Isnaini Ivan Nurizal Sakti Izah, Luluk Lailatul Jatmiko Endro Suseno Jauhari Syamsiyah Joko Winarno Juniyanto, Dimas Kamasita, Systriana Esi Kartini Sinaga Kesawa Sudarsih Khanif Pramusinto, Khanif Kharis, Muhamad Kusworo Adi Kusworowulan, Suryati Kwartarini Murdiastuti, Kwartarini Laela Meitasari, Laela Laska, Yulinda Lukman Hakim Mahda Veronika Maisi, Sri Malau, Rotua Mardiyono Mardiyono Margo Utomo Marsetyawan HNES, Marsetyawan Mediawati, Saskia Mefi Priba Sari, Mefi Priba Melo, Gabriella Imanuella Melyana Nurul Widyawati Menur Wahyu Pangestika, Menur Wahyu Mohamad Nadhif, Mohamad Muh. Yusuf Muhammad Arif Prasetyo Muhammad Arsyad Suyuti Muhammad Izzuddin Shofar Muhammad Zainuri Mujiyo Mujiyo Mulyanto Mulyanto Munarso Munarso Muthoifin Muthoifin, Muthoifin N. Euis Sutaningsih, N. Euis Nailatul Ilmi Nasronudin Nasronudin Niharul Annas Nirwani Soenardjo Nourobby Aulada, Nourobby Oky Dwi Nurhayati Oky Yuripa Pradana P. H, Aditya Praptiwi Praptiwi Prasetyo Nugroho Raden Ario Radini Sinta, Radini Ragil Saputra Rahayu Sapta S Rahmat Gernowo Rakhmat Syaefullah Ramadhan, Hazbina Fauqi Rani Gamawati, Rani Ratna Dwi Meilani, Ratna Dwi Retno Handayani Retno Hartati Ria Amitasari Ria Azizah Rieneke L Sela, Rieneke L Rini Riyanti Robby Eko Christanto Rudhi Pri, Rudhi Rudhi Pribadi Rusdi, Muh Sangkertadi Sangkertadi Satryo Adi Wibowo, Satryo Adi Setyaningsih, Erni Sharfina Sharfina, Sharfina Siti Fatimah Muis Soeharyo Hadisaputro Sri Budi Barunawati, Sri Budi Sri Huning Anwariningsih Sri Kusumastuti, Sri Sri Sedjati Sri Sukamta Sudadi Sudadi Sudibyo Sudibyo Sugeng Purbawanto Sugiyanto Sugiyanto Sukarnen Sukarnen, Sukarnen Sulistyo Warjono, Sulistyo Sumarno Sumarno Sumaryo Sumaryo Suntoro Suntoro Supriyanto Supriyanto Supriyati Supriyati Suroyo Suroyo Suwarto Suwarto Tatyantoro Andrasto Tika Retnowati Tri Saputra, Tri Valentino, Nico Vifaldi Agti, Vifaldi Vincensius Gunawan Wahyu Fredi Santoso Wahyu Setia Budi Widyatmani Sih Dewi Wilis Ari Setyati Yosafat Hermawan Trinugraha, Yosafat Hermawan Yudha Nurdian, Yudha Yuli Hermansyah, Yuli Yuningtyastuti, Gita Surya