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Expression of MRP8/MRP14 mRNA in Monocytes of Periodontitis: Comparison between Diabetic and Non Diabetic Patients

The Indonesian Journal of Dental Research Vol 1, No 1 (2010)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

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Abstract

The severity of periodontitis on patients with type 2 Diabetes Mellitus patients was strongly thought caused by decreasing of leukocytes function such as monoctyes and neutrophils. In our previous research it was found that calprotectin (MRP8/MRP14) level in leukocytes of periodontitis patients with type 2 DM was higher than periodontitis in non DM. The aim of this study was to determine calprotectin (MRP8/MRP14)mRNA expression in human monocytes of periodontitis patients with type 2 DM and without DM. Monocytes were isolated from the peripheral blood of periodontitis patients with uncontrolled type 2 DM, controlled type 2 DM, and non DM. The expression of total RNA calprotectin (MRP8 and MRP14) were detected by RTPCR using GAPDH as the innate control. It was observed that the value of MRP8/MRP14 mRNA expression DM patients were higher than non DM, and the highly significant increase expression (p<0.05) was on the uncontrolled type 2 DM. The basal level of MRP8/MRP14 expression increased in monocyte of periodontitis and type 2 DM patients compared with non diabetes subjects. It was suggested that high basal level MRP8/MRP14 has role in the regulation of severity periodontitis with diabetes mellitus.

The Effect of Visible Light Cure (VLC) Exposure to Gingival Tissue’s Sprague dawley Rats

The Indonesian Journal of Dental Research Vol 1, No 2 (2011)
Publisher : The Indonesian Journal of Dental Research

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Abstract

Visible Light Cure (VLC) is a blue light used in dentistry as an activator for restorative material and fixed orthodontic bonding. The wavelength of VLC is between 400-500 nm and considered non-ionizing radiation that can produce free radicals. According to previous research, the light at wavelength < 500 nm could inhibit cells mitosis, cause cells damage, and reduce cells growth and inflammation. The purpose of this study was to investigate the effect of VLC exposure on gingival epithelial thickness, total neutrophil and macrophage count of gingival connective tissue of Sprague dawley rats. The subjects of this study consisted of 20 Sprague dawley rats, in 2-3 months of age and divided into 4 groups. Each group was 5 rats. The rats in each group were sacrificed before (0 day, as group A) and after 1st (group B), 3rd  (group C), 5th  (group D) day of VLC exposure, respectively. The exposure of VLC was done in labial aspect of cervical anterior teeth of mandible. The distance of exposure was as thick as 2 layers of celluloid strip and the histological specimens were stained by Hematoxylin Eosin. Each specimen was measured for its gingival epithelial thickness by using a micrometer and the number of neutrophil and macrophage were counted. The data of gingival epithelial thickness from 4 groups were analyzed by Kruskal Wallis. The number of neutrophil and macrophage were analyzed by u sing one way ANOVA. The results of this study showed that there were significant differences among groups on the thickness of gingival epithelial, the number of neutrophil and macrophage in the gingival connective tissue of Sprague dawley rats. The result of this study indicated that VLC exposure might decrease the thickness of gingival epithelial but increase the number of neutrophil and macrophage of gingival connective tissue of Sprague dawley rats.

Co-Authors AB Susanto Achmad Widodo Adi Priyo Wicaksono, Adi Priyo Agung Chandra Prasetya Agung Nugroho Setiawan, Agung Nugroho Agus Indarjo Agus Sulistiyo Agus Suyanto Agus Syafrudin Agus Triyanto Ahmad Syaify, Ahmad Aini Moeljono, Aini AL. Supartinah, AL. Andika Bagus Setiawan, Andika Bagus Andri Wahyu Septo Putra Anifatur Nurjannah, Anifatur Arbiyanti, Yossi Norma Arfian, Finty Ari Anshori, Ari Ari Suwondo Arief Suseno, Arief Arif Syaifuddin Arifin Budi Putro, Arifin Budi Arinianzah, Aldo Rizqi Aris Wijayanti Arista, Desi Arlien Siswanti Arnefia Mei Yusnida Asfan Muqtadir, Asfan Astuti, Anjar Ave Gierdo Alfaseno, Ave Gierdo Baliyan I, Baliyan Bambang Nugraha, Bambang Basuki Basuki Bayu Surarso Budi Setiawan Chrisna A Suryono Chrisna Adhi Suryono Cicilia Artitin Cynthia Damayanti Dadang Sukandar Dayyana, Surya Desi Sandra Sari Desie Dwi Wisudanti, Desie Dwi Dewi Agustina Dewi Sulistyaningrum Dhidik Prastiyanto Diana Layla Dimas Putra Anugrah, Dimas Putra Djoko Adi Widodo Donny Prasetyo Santoso, Donny Prasetyo Drajat Martianto Dwi Puji Adiana, Dwi Puji Edi Wibowo Edi Wibowo Kushartono Edy Junaidi Eka Miftakhul Jannah, Eka Miftakhul Eko Supraptono Emi Agustina, Emi Endah Setiyaningrum Endang Sri Susilo ENNY SUSWATI Ervia Yudiati Evi Setiawati Fatekurohman, Mohamat fatimah Fatimah Febriana Tirta Kirana, Febriana Tirta Fitri Mahardika Sari Frida Fallo Gatot Murti Wibowo, Gatot Murti Gatot Yulianto Gentur Handoyo Gigih Budhiawan Gunawan Widi Santosa Hadi Endrawati Harindra W Pradhana, Harindra W Harindra Wisnu Pradhana Haryati Haryati Hayat Maulana Heri Sugito Heri Sutanto Hernita Kusuma Wardani Hery Sulistyo JNS, Hery Sulistyo Hery Widijanto Hidayat, Syarief Thaufik Hilmi Trian Setyawan Indhana Sudiharto, Indhana Indri Trifiantari Irianiwati Irianiwati Irwani Irwani Isa Nurnusanto, Isa Isnaini Isnaini Ivan Nurizal Sakti Izah, Luluk Lailatul Jatmiko Endro Suseno Jauhari Syamsiyah Joko Winarno Juniyanto, Dimas Kamasita, Systriana Esi Kartini Sinaga Kesawa Sudarsih Khanif Pramusinto, Khanif Kharis, Muhamad Kusworo Adi Kusworowulan, Suryati Kwartarini Murdiastuti, Kwartarini Laela Meitasari, Laela Laska, Yulinda Lukman Hakim Mahda Veronika Maisi, Sri Malau, Rotua Mardiyono Mardiyono Margo Utomo Marsetyawan HNES, Marsetyawan Mediawati, Saskia Mefi Priba Sari, Mefi Priba Melo, Gabriella Imanuella Melyana Nurul Widyawati Menur Wahyu Pangestika, Menur Wahyu Mohamad Nadhif, Mohamad Muh. Yusuf Muhammad Arif Prasetyo Muhammad Arsyad Suyuti Muhammad Izzuddin Shofar Muhammad Zainuri Mujiyo Mujiyo Mulyanto Mulyanto Munarso Munarso Muthoifin Muthoifin, Muthoifin N. Euis Sutaningsih, N. Euis Nailatul Ilmi Nasronudin Nasronudin Niharul Annas Nirwani Soenardjo Nourobby Aulada, Nourobby Oky Dwi Nurhayati Oky Yuripa Pradana P. H, Aditya Praptiwi Praptiwi Prasetyo Nugroho Raden Ario Radini Sinta, Radini Ragil Saputra Rahayu Sapta S Rahmat Gernowo Rakhmat Syaefullah Ramadhan, Hazbina Fauqi Rani Gamawati, Rani Ratna Dwi Meilani, Ratna Dwi Retno Handayani Retno Hartati Ria Amitasari Ria Azizah Rieneke L Sela, Rieneke L Rini Riyanti Robby Eko Christanto Rudhi Pri, Rudhi Rudhi Pribadi Rusdi, Muh Sangkertadi Sangkertadi Satryo Adi Wibowo, Satryo Adi Setyaningsih, Erni Sharfina Sharfina, Sharfina Siti Fatimah Muis Soeharyo Hadisaputro Sri Budi Barunawati, Sri Budi Sri Huning Anwariningsih Sri Kusumastuti, Sri Sri Sedjati Sri Sukamta Sudadi Sudadi Sudibyo Sudibyo Sugeng Purbawanto Sugiyanto Sugiyanto Sukarnen Sukarnen, Sukarnen Sulistyo Warjono, Sulistyo Sumarno Sumarno Sumaryo Sumaryo Suntoro Suntoro Supriyanto Supriyanto Supriyati Supriyati Suroyo Suroyo Suwarto Suwarto Tatyantoro Andrasto Tika Retnowati Tri Saputra, Tri Valentino, Nico Vifaldi Agti, Vifaldi Vincensius Gunawan Wahyu Fredi Santoso Wahyu Setia Budi Widyatmani Sih Dewi Wilis Ari Setyati Yosafat Hermawan Trinugraha, Yosafat Hermawan Yudha Nurdian, Yudha Yuli Hermansyah, Yuli Yuningtyastuti, Gita Surya