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THE INFLUENCE OF NIFEDIPINE INDUCTION TO GINGIVAL EPITHELIAL CELL PROLIFERATION (in vivo study in rat)

Journal of Dentistry Indonesia Vol 11, No 1 (2004): April
Publisher : Faculty of Dentistry, University of Indonesia

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Abstract

It is well known that nifedipine administration in hypertensive patients results in gingival hyperplasia. The aim of this study was to study the pattern of nifedipine-induced gingival hyperplasia, based on morphometric and histogical changes as well as on PCNA (Proliferating Cell Nuclear Antigen) expression in the gingival epithelium. In total, 36 male Sprague Dawley rats at the age of 6-8 weeks were divided into nine experimental groups and three control groups. Each animal received daily DMSO (dimethyl sulfoxide) via oral intubation at a dosage of 0 (for control groups), 15, 30, or 60 mg/kg (experimental groups) of body weight for 7, 21 or 42 days. After the animals were sacrificed, impression of the lower gingival tissue was taken to measure mesio-distal distance, labio-lingual distance and papilla height. The number of blood vessels and the thickness of gingival epithelium were assessed from hematoxylin and eosin stained sections. Proliferative activity of the epithelial cells was determined by immunohistochemical analysis using PCNA monoclonal antibody. Significant increase in the mesio-distal and labio-lingual distance of the lower gingival tissue was detected morphometrically (p<0.05). There were more blood vessels in the experimental groups than in the control groups, however there was no specific pattern based on the dosage or duration of nifedipine administration. On the other hand, significant differences were found in the gingival epithelial thickness and proliferative activity between the experimental and the control groups. PCNA-positive cells were observed in basal and suprabasal layers, but nearly none in lamina propria.

Co-Authors AB Susanto Achmad Widodo Adi Priyo Wicaksono, Adi Priyo Agung Chandra Prasetya Agung Nugroho Setiawan, Agung Nugroho Agus Indarjo Agus Sulistiyo Agus Suyanto Agus Syafrudin Agus Triyanto Ahmad Syaify, Ahmad Aini Moeljono, Aini AL. Supartinah, AL. Andika Bagus Setiawan, Andika Bagus Andri Wahyu Septo Putra Anifatur Nurjannah, Anifatur Arbiyanti, Yossi Norma Arfian, Finty Ari Anshori, Ari Ari Suwondo Arief Suseno, Arief Arif Syaifuddin Arifin Budi Putro, Arifin Budi Arinianzah, Aldo Rizqi Aris Wijayanti Arista, Desi Arlien Siswanti Arnefia Mei Yusnida Asfan Muqtadir, Asfan Astuti, Anjar Ave Gierdo Alfaseno, Ave Gierdo Baliyan I, Baliyan Bambang Nugraha, Bambang Basuki Basuki Bayu Surarso Budi Setiawan Chrisna A Suryono Chrisna Adhi Suryono Cicilia Artitin Cynthia Damayanti Dadang Sukandar Dayyana, Surya Desi Sandra Sari Desie Dwi Wisudanti, Desie Dwi Dewi Agustina Dewi Sulistyaningrum Dhidik Prastiyanto Diana Layla Dimas Putra Anugrah, Dimas Putra Djoko Adi Widodo Donny Prasetyo Santoso, Donny Prasetyo Drajat Martianto Dwi Puji Adiana, Dwi Puji Edi Wibowo Edi Wibowo Kushartono Edy Junaidi Eka Miftakhul Jannah, Eka Miftakhul Eko Supraptono Emi Agustina, Emi Endah Setiyaningrum Endang Sri Susilo ENNY SUSWATI Ervia Yudiati Evi Setiawati Fatekurohman, Mohamat fatimah Fatimah Febriana Tirta Kirana, Febriana Tirta Fitri Mahardika Sari Frida Fallo Gatot Murti Wibowo, Gatot Murti Gatot Yulianto Gentur Handoyo Gigih Budhiawan Gunawan Widi Santosa Hadi Endrawati Harindra W Pradhana, Harindra W Harindra Wisnu Pradhana Haryati Haryati Hayat Maulana Heri Sugito Heri Sutanto Hernita Kusuma Wardani Hery Sulistyo JNS, Hery Sulistyo Hery Widijanto Hidayat, Syarief Thaufik Hilmi Trian Setyawan Indhana Sudiharto, Indhana Indri Trifiantari Irianiwati Irianiwati Irwani Irwani Isa Nurnusanto, Isa Isnaini Isnaini Ivan Nurizal Sakti Izah, Luluk Lailatul Jatmiko Endro Suseno Jauhari Syamsiyah Joko Winarno Juniyanto, Dimas Kamasita, Systriana Esi Kartini Sinaga Kesawa Sudarsih Khanif Pramusinto, Khanif Kharis, Muhamad Kusworo Adi Kusworowulan, Suryati Kwartarini Murdiastuti, Kwartarini Laela Meitasari, Laela Laska, Yulinda Lukman Hakim Mahda Veronika Maisi, Sri Malau, Rotua Mardiyono Mardiyono Margo Utomo Marsetyawan HNES, Marsetyawan Mediawati, Saskia Mefi Priba Sari, Mefi Priba Melo, Gabriella Imanuella Melyana Nurul Widyawati Menur Wahyu Pangestika, Menur Wahyu Mohamad Nadhif, Mohamad Muh. Yusuf Muhammad Arif Prasetyo Muhammad Arsyad Suyuti Muhammad Izzuddin Shofar Muhammad Zainuri Mujiyo Mujiyo Mulyanto Mulyanto Munarso Munarso Muthoifin Muthoifin, Muthoifin N. Euis Sutaningsih, N. Euis Nailatul Ilmi Nasronudin Nasronudin Niharul Annas Nirwani Soenardjo Nourobby Aulada, Nourobby Oky Dwi Nurhayati Oky Yuripa Pradana P. H, Aditya Praptiwi Praptiwi Prasetyo Nugroho Raden Ario Radini Sinta, Radini Ragil Saputra Rahayu Sapta S Rahmat Gernowo Rakhmat Syaefullah Ramadhan, Hazbina Fauqi Rani Gamawati, Rani Ratna Dwi Meilani, Ratna Dwi Retno Handayani Retno Hartati Ria Amitasari Ria Azizah Rieneke L Sela, Rieneke L Rini Riyanti Robby Eko Christanto Rudhi Pri, Rudhi Rudhi Pribadi Rusdi, Muh Sangkertadi Sangkertadi Satryo Adi Wibowo, Satryo Adi Setyaningsih, Erni Sharfina Sharfina, Sharfina Siti Fatimah Muis Soeharyo Hadisaputro Sri Budi Barunawati, Sri Budi Sri Huning Anwariningsih Sri Kusumastuti, Sri Sri Sedjati Sri Sukamta Sudadi Sudadi Sudibyo Sudibyo Sugeng Purbawanto Sugiyanto Sugiyanto Sukarnen Sukarnen, Sukarnen Sulistyo Warjono, Sulistyo Sumarno Sumarno Sumaryo Sumaryo Suntoro Suntoro Supriyanto Supriyanto Supriyati Supriyati Suroyo Suroyo Suwarto Suwarto Tatyantoro Andrasto Tika Retnowati Tri Saputra, Tri Valentino, Nico Vifaldi Agti, Vifaldi Vincensius Gunawan Wahyu Fredi Santoso Wahyu Setia Budi Widyatmani Sih Dewi Wilis Ari Setyati Yosafat Hermawan Trinugraha, Yosafat Hermawan Yudha Nurdian, Yudha Yuli Hermansyah, Yuli Yuningtyastuti, Gita Surya