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BIOSORPSI CAMPURAN LOGAM Pb2+ DAN Zn2+ OLEH Chaetoceros calcitrans

CHEMISTRY PROGRESS Vol 5, No 2 (2012)
Publisher : Sam Ratulangi University

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Abstract

Penelitian ini bertujuan untuk menentukan dampak jerapan campuran ion logam Pb(II) dan Zn(II) terhadap pertumbuhan mikroalga Chaetoceros calcitrans dan menentukan efisiensi penjerapan kedua ion logam dalam campuran. Pemaparan ion logam dengan berbagai variasi konsentrasi Pb(II) terhadap Zn(II)dilakukan setelah diperoleh pertumbuhan optimum C. calcitrans, yakni pada hari ke-9. Konsentrasi ion logam Pb(II) dan Zn(II) setelah pemaparan ditentukan dengan spektrofotometer serapan atom. Penambahan ion Pb(II) ke dalam Zn(II)membuat penjerapan ion Zn(II) oleh C. calcitrans turun dibandingkan dengan penjerapan ion tunggal Zn(II). Hal yang sama juga terjadi pada penambahan ion Zn(II)ke dalam ion Pb(II). Hasil penelitian menunjukkan bahwa ion Pb(II) lebih banyak terjerap oleh C. calcitrans dibanding ion Zn(II). Effisiensi penjerapan optimum ion Pb(II) sebesar 64,44% pada perbandingan konsentrasi Pb(II) terhadap Zn(II) 45 : 30 ppm sedangkan penjerapan ion Zn(II) yaitu 56,33% pada perbandingan konsentrasi Zn(II) terhadap Pb(II) 15 : 30 ppm.The main purpose of this research was to determine the adsorption effect of the mixture of Pb(II) and Zn(II) on the growth of Chaetoceros calcitrans and to determine the adsorption efficiency of the ions in the mixture. Exposure of Pb(II) and Zn(II)ions was conducted after the optimum growth of C. calcitrans was obtained, that is at the ninth day with the variation of the concentration ratio of Pb(II) to Zn(II). Concentration of Pb(II) and Zn(II) ions after exposure was determined by atomic absorption spectrophotometer. Addition of Pb(II) ion in solution Zn(II) ion resulted in the decrease of the Zn(II)ion adsorbed by C. calcitrans compared to the adsorption of the single ion of Zn(II). The same result was obtained when Zn(II) ion was added in Pb(II) solution. Results showed that the adsorption of Pb2+ ion by C. calcitrans was higher than that of Zn(II) ion. The maximum adsorption efficiency of Pb(II) ion was 64.44% at the Pb(II):Zn(II) ratio of 45:30, whereas that of Zn(II)ion was 56.33% at the Zn(II):Pb(II) ratio of 15:30.

PENAPISAN DAN ANALISIS SPONGE EFEKTIF SEBAGAI ANTIBIOFOULING DI TAMBAK DAN KERAMBA JARING APUNG

MARINA CHIMICA ACTA 1999
Publisher : Universitas Hasanuddin

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Abstract

Crude extracts of sponge has been screened for its antibiofouling properties in fishpond and keramba floating net through several stages such as isolation, identification of attached organisms, bioactivity test of sponge crude extract using asbetic plate, 10 x 5 cm, coated with the extract. Those showing  antibiofouling activity is further tested of its toxicity upon larvae (Balanus amphitrite). The amount of organisms attached on asbetic plate is an activity indicator of sponge extract crude. Field observation was conducted for 40 days with 10 day interval. The result of sponge bioactive screening on attached organisms at floating net provide 8 species among others are; Asterospus sp, Clathria sp, Clathria reinwardi, Desmopasma sp, Dysidea sp,  Haliclona sp, Pericarax sp, dan  Jaspis sp, while in fishpond 4 species i.e;  Clathria sp,  Halichondria sp, Callyspongia sp, and  Jaspis sp  with value of LC50 in 24 consecutive hours as follows:  27,99 ppm; 87,57 ppm ; 112,62 ppm;  dan 117,63 ppm. Extract of  Clathria sp showed highest toxicity and its active fraction is in crude extract while  Halichondria sp is more in its aquoeus fraction. These bioactive components were estimated coming from terpenoid and phenolic acid derivatives.   Key words : Sponge, bioactive, antibiofouling, fishpond, keramba

INTERAKSI ION Cu(II) DENGAN Chaetoceros Calcitrans DALAM LINGKUNGAN PERAIRAN LAUT

MARINA CHIMICA ACTA Vol 5, No 2
Publisher : Universitas Hasanuddin

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Abstract

Penelitian tentang interaksi ion Cu(II) dengan Chaetoceros calcitrans di lingkungan perairan laut telah dilakukan. Tahapan kerja yang dilakukan meliputi penyiapan fitoplankton uji C. calcitrans yang diisolasi dari alam lalu dikultur murni dalam medium Conwy, dan pemaparan berbagai konsentrasi ion Cu(II) untuk mengetahui pola pertumbuhan C. calcitrans. Hasil yang diperoleh yaitu pertumbuhan optimum C. calcitrans diperoleh pada hari ke-9, sedangkan dampak pemaparan ion Cu(II) menunjukkan tren penurunan populasi berbanding lurus dengan kenaikan konsentrasi ion Cu(II) dengan pola yang bervariasi fungsi waktu pemeliharaan. Hasil pengukuran spektrofotometri serapan atom terhadap konsentrasi ion Cu(II) pada filtrat cenderung meningkat sejalan dengan kenaikan ketersediaan ion di dalam medium pertumbuhan.   Kata kunci : Interaksi, fitoplankton, tembaga(II), laut.

EKSTRAKSI DNA RUMPUT LAUT Kappaphycus alvarezii DENGAN METODE FENOL KLOROFORM

MARINA CHIMICA ACTA Vol 2
Publisher : Universitas Hasanuddin

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Abstract

Telah dilakukan ekstraksi genom DNA rumput laut Kappaphycus  alvarezii dengan metode konvensional fenol kloroform dengan modifikasi. Sampel yang digunakan berasal dari lokasi budidaya di Pinrang, Madura dan Lombok. Hasil ekstraksi memperlihatkan pita genom DNA yang relatif bersih.  Penambahan kalium asetat dapat meningkatkan kemurnian genom DNA yang diekstraksi. Pengukuran konsentrasi dan kemurnian genom DNA dilakukan dengan menggunakan Spektrofotmeter UV-VIS, diperoleh konsentrasi DNA sekitar 180-240 ng/mL dengan tingkat kemurnian rata-rata 1,89. Hasil elektroforesis menunjukkan bahwa  konsentrasi DNA 1.020 ng cukup untuk memperlihatkan pita yang jelas.   Kata Kunci :  Ekstraksi DNA, Kappaphycus  alvarezii , Fenol kloroform

BACTERIAL DISEASES PREVENTION ON SHRIMP (PENAEUS MONODON) USING MANGROVE BIOACTIVE (AVICENIA ALBA)

MARINA CHIMICA ACTA Vol 2
Publisher : Universitas Hasanuddin

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Abstract

Avicenia alba is one of mangrove ecosystem reported containing glicoside, terpen and amino acid substant which could be utilized as anticancer, infection and analgetic. The study aims to find out the bioactive which are effective to reduce bacterial diseases on shrimp culture. This research is conducted to showed probability of the using of bioactive isolat from Avicenia alba as bactericide on shrimp culture the bioactive activity was tested with sensitivity test using using several bacteria of vibrio sp isolated from ponds and infected shrimp.  The Isolation of mangrove bioactive was carry out  by extraction, fractination, and purification methods.  The extract was fractination by chromatography by using solvents of different polarity, such as methanol, hexane, ethyl acetate, and water.  The HPLC analysis was conducted on isolate to test its purification and identification of bioactive subtance of Avicenia alba uses spectroscopic, spot reaction on TLC and GC-MS.  The result of crude extract analysis of Avicenia alba showed inhibition zone on Vibrio leognathy and Vibrio harveyi are 15.5 ±0.04 and 13.7±0.04mm of extract containing 1g/mL fresh weight.  The result of purification and identification showed that the isolates give a respon to amine compound. The result of identification with GC-MS matches to 2-isocyanato-2-hexanamine.Keyword : Bioactive, mangrove , bactericide

BIOACTIVE SUBSTANCES OF MANGROVE Excoecaria agallocha AS SHRIMP DISEASES INHIBITOR

MARINA CHIMICA ACTA Vol 2
Publisher : Universitas Hasanuddin

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Abstract

Excoecaria agallocha mangrove species is suspected of having bioactive material which can be used as bacteriostatic agent, water nutrient stability and shrimp diseases reduction.  Hexane, acid ethyl acetate, neutral ethyl acetate, and water were used  as solvents.  Chemical compounds of  mangrove bioactive were classified by color  reaction on  TLC and identified by spectroscopic  method.  The highest inhibition zone  crude extract of bioactive mangrove toward several Vibrio sp. from the shrimp ponds show that V. mimicus are 10.35 ± 0.05 mm at 10 µL and 12.6 ± 0.05 at 20 µL.  The highes inhibition zone produce by fraction Et-OAC neutral  with V. costicola are 18.3 ± 0.05 at 10 µL and 23.9 ± 0.05  at 20 µL   Keywords: Bioactive, mangrove , bactericide

ISOLASI BIOAKTIF HYDROZOAN Lytocarpus philippinus SEBAGAI BAKTERISIDA PADA UDANG

MARINA CHIMICA ACTA Vol 1
Publisher : Universitas Hasanuddin

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Abstract

Lytocarpus philippinus, a  hydrozoan species, commonly found in coral reefs is suspected of having bioactive material which can be used as bactericide.   The experiment was aimed at finding out the hydrozoan bioactive which could inhibit the growth of  Pseudomonas sp isolated from diseases tiger prawn. The analysis was conducted using extraction, fractionation, and purification methods.   Hexane, acid ethyl acetate, neutral ethyl acetate, and water were used  as solvents.  Chemical compounds of hydrozoan bioactive were classified by color  reaction on  thin layer chromatography and identified by spectroscopic  methods.   The  bioactive extracted from   Lytocarpus philippinus is suspected to be n-Cyclohexil-3-beta-methoxy-4-methyliden 451 compound.   Keywords: Bioactive, hydrozoan , bactericide

The effects of stimulant growth hormones on tissue culture of seaweed Kappaphycus alvarezii in vitro

AQUATIC SCIENCE & MANAGEMENT (Jurnal Ilmu dan Manajemen Perairan) Edisi Khusus 1 (2013): Mei
Publisher : Pascasarjana, Universitas Sam Ratulangi

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Abstract

In order to anticipate the qualified and sustainable seed requirement for seaweed culture, it is necessary to conduct tissue culture for vegetative cultivation of isolated leaves, bud, and stemin an artificial medium enriched with nutrient and growth regulator. The purpose of this study is to obtain newly grown plant in a big quantity in relatively short period of time, with physiological and morphological properties similar to the stocks. Culture media used were Grund Medium and PES with an addition of a growth regulator, IAA (Indol acetic acid) and BAP (Benzil amino purin). The buds produced were buds with similar properties as the parent. The longest bud (1,851 mm) was obtained in Grund Medium with IAA treatment, while the length of bud in PES medium was only 0.612 mm. The number of buds was highest (10,6)  in Grund media with IAA+BAP (1:1) treatment, and 6,82 with IAA treatment in PES media. The survival rate of explants was highest in media enriched with 0.5 mg/L IAA (indol acetic acid). The best media for growing seaweed Kappaphycus alvarezii was Grund Medium© Untuk mengantisipasi kebutuhan bibit yang berkualitas dan tersedia  secara kontinyu, diperlukan suatu upaya kultur jaringan untuk perbanyakan tanaman secara vegetatif dengan mengisolasi bagian tanaman seperti daun, mata tunas, serta batang dalam media buatan secara aseptik yang diperkaya dengan nutrien dan zat perangsang tumbuh. Tujuannya untuk mendapatkan tanaman baru dalam jumlah banyak dalam waktu yang relatif singkat, yang mempunyai sifat fisiologi dan morfologis sama dengan tanaman induknya. Media kultur yang digunakan adalah media Grund Medium dan PES dengan penambahan zat perangsang tumbuh yaitu IAA (Indol acetic acid) dan BAP (Benzil amino purin). Tunas yang dihasilkan merupakan anakan yang mempunyai sifat yang sama dengan induknya.  Panjang tunas tertinggi dicapai pada media Grund Medium dengan perlakuan IAA (1,851 mm) dan media PES sebesar 0,612 mm. Sedangkan jumlah tunas tertinggi dicapai perlakuan IAA+BAP (1:1) sebesar 10,6 pada media Grund dan perlakuan IAA sebesar 6,82 pada media PES. Untuk tingkat kelangsungan hidup (sintasan) eksplan yang paling baik pada media yang diberikan pupuk IAA (indol acetic acit) dengan kosentrasi 0,5 mg/L. sedangkan media yang baik untuk pertumbuhan rumput laut Kappaphycus alvarezii adalah media Grund Medium©

Binary Vector Construction and Agrobacterium tumefaciens-mediated Transformation of Lysozyme Gene in Seaweed Kappaphycus alvarezii

BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol 21, No 2 (2014)
Publisher : SEAMEO BIOTROP

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Abstract

Ice-ice disease is the biggest problem in the cultivation of seaweed Kappaphycus alvarezii. The disease is caused by bacterial infection and induced by drastic changes of water quality. Lysozyme has the ability to break down bacterial cell wall. The purpose of this research was to construct of a binary vector pMSH1-Lys carrying chicken Lysozyme (Lis) gene and introduce pMSH1-Lis on K. alvarezii. The binary vector expression was transformed into Agrobacterium tumefaciens LBA4404 by triparental mating. Thallus was inoculated with A. tumefaciens carrying pMSH1-Lis and then the transformed thallus was selected by adding 20 mg/L hygromycin to the culture medium. PCR analysis showed that the construction of the binary plasmid pMSH1-Lys was established. Percentage of transformation of pMSH1-Lis on K. alvarezii was 23.56%, while the efficiency of regeneration was 11.32%. PCR analysis showed that three of the regenerated thallus contained Lysozyme gene. Thus, transgenic K. alvarezii was produced successfully and this can be useful for studying the mechanisms of seaweed defense against bacterial infection.

Pertumbuhan dan Perkembangan Eksplan Rumput Laut Gracilaria verrucosa dan Gracilaria gigas pada Aklimatisasi di Tambak

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 3 (2015): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Aklimatisasi eksplan rumput laut hasil kultur jaringan merupakan proses adaptasi eksplan dengan lingkungan budidaya. Penelitian ini bertujuan untuk mengevaluasi performa pertumbuhan dan perkembangan eksplan rumput laut G. verrucosa dan G. gigas yang diaklimatisasi di tambak dan mendapatkan informasi awal mengenai prospek pengembangan budidaya rumput laut G. gigas di tambak. Eksplan rumput laut G. verrucosa dan G. gigas hasil kultur jaringan dipelihara dalam hapa berukuran 50x50x50 cm dengan berat awal 15 g.hapa-1 dan dipelihara di tambak. Desain penelitian adalah rancangan acak lengkap (RAL) dengan enam unit penelitian terdiri dari tiga ulangan untuk masing-masing spesies. Pemeliharaan eksplan dilakukan selama 60 hari dan setiap 15 hari dilakukan pengukuran bobot, panjang dan perkembangan eksplan serta monitoring terhadap kualitas air. Pengamatan histologi sel rumput laut G. verrucosa dan G. gigas dilakukan dibawah mikroskop. Analisis data pertumbuhan dilakukan dengan uji komparatif independent t-test sedangkan data perkembangan eksplan dan histologi sel rumput laut dianalisis secara deskritif. Pada pemeliharaan di tambak kedua jenis rumput laut memiliki pertumbuhan yang berbeda nyata (P<0,05). Rumput laut G. verrucosa memiliki bobot mutlak lebih tinggi (221,82 g) dari G. gigas (51,94 g) dan LPH (laju pertumbuhan harian) bobot lebih tinggi (3,27%) dari G. gigas (2%). Rumput laut G. verrucosa juga memiliki pertambahan panjang yang lebih tinggi (5,28 cm) dari G. gigas (2,71 cm) dengan LPH panjang masing-masing sebesar 3,06% dan 2,18%. Perkembangan eksplan rumput laut G. verrucosa lebih cepat daripada G. gigas karena faktor fisika dan kimia lingkungan perairan tambak yang tidak sesuai untuk pertumbuhan rumput laut G. gigas yang memiliki susunan sel korteks lebih rapat. Kata kunci: pertumbuhan, perkembangan, G. verrucosa, G. gigas, eksplan, tambak Acclimatization of tissue culture seaweed explants was an adaptation procces of explants to cultivation environment. This study aims to evaluate the growth and development of G. verrucosa dan G. gigas explants on pond acclimatization as early information of pond aquaculture development of G. gigas. Explants of G. verrucosa and G. gigas were rearing on 50x50x50 cm cage net with 15 g.cage-1 of initial weight and cutured on pond. The study was a completely randomized design with six unit experiment including three replicates for each species. Acclimation was done in 60 days then explants weight, length, development, and water quality were monitored every 15 days. G. verrucosa and G. gigas cell histology was observed under microscope. Growth data was analyzed comparatively using independent t-test then explants development and cell histology were represented descriptively. The study showed that the growth of both species was significantly different (P<0.05) on pond cultivation. G. verrucosa had higher weight (221.82 g) than G. gigas (51.94 g) also higher DGR (daily growth rate) (3.27%) than G. gigas (2%). G. verrucosa also had higher elongation (5.28 cm) than G. gigas (2.71 cm) with length DGR of 3.06% and 2.18%, respectively. The development of G. verrucosa explants was better than G. gigas, because of the physical and chemical environment of pond water was not suitable for G. gigas which had dense cortical structure. Keywords: growth, development, G. verrucosa, G. gigas, explants, pond