Wardhana Suryapratama
Fakultas Peternakan Universitas Jenderal Soedirman
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UPAYA PENINGKATAN KUALITAS KULIT SINGKONG MELALUI FERMENTASI MENGGUNAKAN LEUCONOSTOC MESENTEROIDES PENGARUHNYA TERHADAP KECERNAAN BAHAN KERING DAN BAHAN ORGANIK SECARA IN VITRO

Jurnal Ilmiah Peternakan Vol 1, No 1 (2013): Jurnal Ilmiah Peternakan
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ABSTRAK Penelitian bertujuan mengkaji penggunaan kulit singkong yang di fermentasi menggunakan Leuconostoc mesenteriodes terhadap kecernaan bahan kering (KBK) dan bahan organik (KBO) pakan kambing. Materi yang digunakan dalam penelitian yaitu cairan rumen kambing yang diambil dari Rumah Potong Hewan (RPH) Karanglewas Purwokerto. Penelitian dilakukan secara eksperimental yang dirancang menggunakan Rancangan Acak Lengkap (RAL). Perlakuan adalah konsentrat tanpa kulit singkong fermentasi (P1), konsentrat yang di tambahkan 10% kulit singkong fermentasi (P2), konsentrat yang di tambahkan 20% kulit singkong fermentasi (P3), dan konsentrat yang di tambahkan 30% kulit singkong fermentasi (P4). Kulit singkong yang di fermentasi menggunakan Leuconostoc mesenteriodes selama 4 minggu di gunakan sebagai pengganti bekatul dalam konsentrat. Pakan terdiri atas rumput lapang dan konsentrat dengan imbangan 60 : 40. Variabel yang diukur yaitu kecernan bahan kering dan kecernaan bahan organik. Data yang diperoleh dianalisis dengan analisis variansi. Hasil penelitian diperoleh rataan KBK dan KBO untuk masing-masing perlakuan P1, P2, P3 dan P4 adalah 47,59%; 42,26%, 47,26%; 40,60%, 44,31,%; 39,76% dan 48,91%; 44,91%. Analisis variansi menunjukkan bahwa perlakuan tidak berbeda nyata (P>0,05) baik terhadap kecernaan bahan kering maupun bahan organik. Kulit singkong yang di fermentasi secara anaerob menggunakan L. Mesenteroides dapat menggantikan bekatul dalam pakan kambing. Kata kunci : Kulit Singkong, Leuconostoc mesenteroides, Kecernaan Bahan Kering, kecernaan Bahan Organik ABSTRACT The aim of this research was to assessing the use of cassava peel using Leuconostoc mesenteriodes the digestibility of dry matter and organic matter of cassava peel silage. The materials used in this research were goat rumen fluid taken from slaughter house of Karangluwas Purwokerto. This experiment used experimental method design according to Completely Randomized Design. The treatments tested were unfermented cassava peel as control (P1), while for P2, P3 and P4 same as P1, but adding of coupled with fermented cassava peel using Leuconostoc mesenteroides 10%, 20%, 30% respectively. Feed consists of grass field and concentrate the balance 60: 40. The variable measured and recorded were Dry Matter Digestibility (DMD) and Organic Matter Digestibility (OMD). Data were analysed by anava and continued Orthogonal Polinomial. The result showed that the average of digestible dry matter and organic matter digestibility were 47,59%; 42,26%, 47,26%; 40,60%, 44,31%; 39,76% dan 48,91%; 44,91% for P1, P2, P3 and P4 respectively.  Analysis of variance showed that treatment were no significant effect (P > 0.05) on dry matter and organic matter digestibility. It can be concluded that Cassava peel silage which uses L. mesenteroides can replace rice bran in goat feed Keywords: Cassava Peel, Leuconostoc  Mesenteroides, Dry Matter Digestibility, Organic Matter Digestibility

PENGUJIAN BAHAN PAKAN BUNGKIL BIJI JARAK FERMENTASI SECARA IN VITRO DITINJAU DARI KECERNAAN PROTEIN KASAR DAN SERAT KASAR

Jurnal Ilmiah Peternakan Vol 1, No 2 (2013): Jurnal Ilmiah Peternakan
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Penelitian berjudul Pengujian Bahan Pakan Bungkil Biji Jarak Fermentasi Secara In Vitro  Ditinjau dari Kecernaan Protein Kasar dan Serat Kasar telah dilaksanakan pada 10 Desember 2012 sampai 10 Januari 2013. Materi yang digunakan dalam penelitian adalah bungkil bji jarak, bakteri Lactobacillus acidophyllus dan Bifidobacterium bifidum serta cairan rumen sapi potong. Metode penelitian dilakukan eksperimental secara in vitro menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 5  ulangan yaitu R0 = bungkil biji jarak tanpa fermentasi, R1= bungkil biji jarak fermentasi dengan Lactobacillus acidophyllus, R2= bungkil biji jarak fermentasi dengan Bifidobacterium bifidum, R3= bungkil biji jarak fermentasi dengan  Lactobacillus acidophyllus dan Bifidobacterium bifidum. Peubah yang diamati adalah kecernaan protein kasar dan serat kasar serta dianalisis menggunakan analisis variansi dan dilanjutkan uji orthogonal kontras dengan set kontras: 1). R0 vs R1R2R3, 2). R1 vs R2, dan 3). R1R2 vs R3. Hasil penelitian menunjukkan bahwa penggunaan bungkil biji jarak yang difermentasi dengan bakteri asam laktat berpengaruh secara nyata (P<0,01) menurunkan tingkat kecernaan protein kasar dan serat kasar. Penurunan nilai kecernaan menunjukkan bahwa bakteri asam laktat dapat memproteksi protein dalam bungkil biji jarak sehingga dapat lolos dari degradasi mikroba rumen dan dapat digunakan sebagai protein by pass. Hasil uji lanjut orthogonal kontras menunjukkan bahwa perlakuan R0 berbeda nyata terhadap R1, R2, dan R3. Hal ini menandakan bahwa penggunaan bakteri asam laktat mempengaruhi tingkat kecernaan di rumen. Uji orthogonal kontras dengan set kontras R1 vs R2, dan R1R2 vs R3 tidak menunjukkan perbedaan, yang artinya jenis bakteri asam laktat tidak mempengaruhi tingkat kecernaan protein kasar dan serat kasar. Kesimpulan dari penelitian ini adalah bungkil biji jarak fermentasi dengan bakteri asam laktat (Lactobacillus acidophyllus dan Bifidobacterium bifidum) menurunkan tingkat kecernaan protein kasar dan serat kasar. Akan tetapi, tidak menurunkan kualitasnya sebagai bahan pakan sumber protein.Kata kunci: bungkil biji jarak; kecernaan protein kasar; kecernaan serat kasar; bakteri asam laktat.A research entitled The Test of Fermented Jatropha curcas L. Seed Meal by In Vitro Viewed from Crude Protein and Crude Fiber Digestion. This research was held from December 10th 2012 until January 10th 2013. The materials of this research were Jatropha curcas L. seed meal, MRSB media, molasses, Lactobacillus acidophyllus dan Bifidobacterium bifidum bacteria starter and rumen fluid of beef cattle. The research method was experimental by in vitro using completely randomized design (CRD). The treatments were R0= Jatropha curcas L. seed meal without fermentation, R1= fermented of Jatropha curcas L. seed meal with Lactobacillus acidophyllus, R2= fermented of Jatropha curcas L. seed meal with Bifidobacterium bifidum, R3= fermented of Jatropha curcas L. seed meal with combination of Lactobacillus acidophyllus and Bifidobacterium bifidum. The variables observed were crude protein and crude fiber digestibility, data were analyzed using analysis of variance and followed by orthogonal contrasts of which contrast set were: 1). R0 vs R1R2R3, 2). R1 vs R2, dan 3). R1R2 vs R3. The results showed that the values of crude protein digestion were 81.63%; 74.61%; 70.75%; 75.10%. The values of fiber digestion were 74.39%; 60.52%; 58.16; 59.25. These results showed that the use of fermented Jatropha curcas L. seed meal with lactic acid bacteria significantly reduced (P<0.01) the crude protein and crude fiber digestibility. This reduced digestibility showed that lactic acid bacteria can protect the protein in the Jatropha curcas L. seed meal so that passed by rumen degradation and can be used as a protein by pass. Orthogonal contrast test results showed that the treatment R0 were significantly different from R1, R2, dan R3. It showed that the use of lactic acid bacteria in fermentation affect the level of digestibility. Orthogonal test with contrast set R1 vs R2, and R1R2 vs R3 were not different, which meant that the type of lactic acid bacteria did not affect the level of digestibility. The conclusion of this research is the fermented Jatropha curcas L. seed meal significantly reduces the crude protein and crude fiber digestibility, but it does not reduce the quality of feed as a source of protein.Keywords: Jatropha curcas L. seed meal, crude protein and crude fiber digestion, lactic acid bacteria.

FERMENTASI AMPAS TEBU (BAGASSE) MENGGUNAKAN Phanerochaete chrysosporium SEBAGAI UPAYA MENINGKATKAN PRODUK FERMENTASI RUMEN SECARA IN VITRO

Jurnal Ilmiah Peternakan Vol 1, No 2 (2013): Jurnal Ilmiah Peternakan
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Penelitian berjudul Fermentasi ampas tebu (bagasse) menggunakan Phanerochaete chrysosporium sebagai upaya meningkatkan produk fermentasi rumen Secara In vitro dilaksanakan pada Januari sampai April 2013. Materi yang digunakan dalam penelitian ini adalah cairan rumen sapi potong dan pakan kontrol ampas tebu : konsentrat (40:60) dengan susunan konsentrat yaitu 28% bungkil kelapa, 30% dedak, 1% garam dan 1% kapur (CaCO3). Metode penelitian adalah eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 5 ulangan yaitu A0 : Pakan yang mengandung ampas tebu tanpa fermentasi (Phanerochaete chrysosporium 0 gram), A1 : Pakan yang mengandung ampas tebu fermentasi menggunakan 5 gram Phanerochaete chrysosporium per kg ampas tebu (BK), A2 : Pakan yang mengandung ampas tebu fermentasi menggunakan 10 gram Phanerochaete chrysosporium per kg ampas tebu (BK), A3 : Pakan yang mengandung ampas tebu fermentasi menggunakan 15 gram Phanerochaete chrysosporium per kg ampas tebu (BK). Data dianalisis menggunakan analisis variansi dan dilanjutkan uji Orthogonal Polinomial. Hasil penelitian menunjukan bahwa perlakuan berpengaruh secara linear terhadap konsentrasi VFA dengan persamaan Y= 2.384 X + 64.72, r2 = 0.65, dan berpengaruh secara linear terhadap Sintesis Protein Mikroba rumen dengan persamaan Y= 0.5376 X + 19.808, r2 = 0.53, namun tidak berpengaruh terhadap konsentrasi NH3 (P>0,05). Kesimpulan dari penelitian ini adalah bahwa semakin tinggi taraf Phanerochaete chrysosporium (15 g/kg BK ampas tebu) dalam fermentasi ampas tebu semakin tinggi konsentrasi VFA dan Sintesis Protein Mikroba rumen. Hasil terbaik dari penelitian ini dicapai pada pakan yang difermentasi menggunakan Phanerochaete chrysosporium 15 gr/kg BK ampas tebu (A3). Kata Kunci :  Phanerochaete chrysosporium, Fermentasi, Konsentrasi VFA Total, NH3 serta Sintesis Protein Mikroba Rumen. A research entitled sugarcane bagasse fermentation using Phanerochaete chrysosporium as effort to increase rumen fermentation product on in vitro was conducted from January to April 2013. The materials of the research were rumen fluid of beef cattle and control of feed sugarcane bagasse : consentrate (40:60) with concentrate formulation was 28% coconut meal, 30% rice bran, 1% Salt, and 1% Limestone (CaCO3). The research method was experimentaly using Completely Randomized Design (CRD) with 4 treatments and 5 replicates. Treatments were A0 = Feed contained sugarcane bagasse without fermentation, A1 = Feed contained sugarcane bagasse fermented with 5 gram Phanerochaete chrysosporium (g/Kg DM), A2 = Feed contained sugarcane bagasse fermented with 10 gram Phanerochaete chrysosporium (g/Kg DM), A3 = Feed contained sugarcane bagasse fermented with 15 gram Phanerochaete chrysosporium (g/Kg DM). Data were analyzed using analysis of variance and continued by orthogonal polinomial test. The effects on total VFA concentration and ruminal microbial protein synthesis were linear with equation Y= 2.384 X + 64.72, r2 = 0.65  and Y= 0.5376 X + 19.808, r2 = 0.53 respectivelly. However, treatment fermentation on NH3 concentration was not significant (P>0.05). In conclusions of the research is the higher the level of fermentation of sugarcane bagasse using Phanerochaete chrysosporium the higher total VFA concentration and ruminal microbial protein synthesis. The best resultin this research was the treatment feed contained sugarcane bagasse fermented with 15 gram Phanerochaete chrysosporium (g/Kg DM).Keywords : Phanerochaete chrysosporium, Fermentation, Total VFA Concentration, NH3 and rumen microbial protein synthesis.

IMBANGAN PUCUK TEBU DAN AMPAS TEBU YANG DIFERMENTASI MENGGUNAKAN Phanerochaete chrysosporium PENGARUHNYA TERHADAP PRODUK FERMENTASI RUMEN

Jurnal Ilmiah Peternakan Vol 1, No 2 (2013): Jurnal Ilmiah Peternakan
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Penelitian berjudul imbangan pucuk tebu dan ampas tebu yang difermentasi menggunakan Phanerochaete chrysosporium pengaruhnya terhadap produk fermentasi rumen telah dilaksanakan pada Desember 2012 sampai Maret 2013. Materi yang digunakan dalam penelitian adalah cairan rumen sapi potong dan pakan terdiri atas konsentrat dan campuran pucuk tebu dan ampas tebu dengan imbangan 60:40. Campuran pucuk tebu dan ampas tebu difermentasi menggunakan jamur Phanerochaete chrysosporium 10g/kg campuran pucuk tebu dan ampas tebu. Konsentrat tersusun dari14% jagung giling, 40% dedak, 5% onggok, 20% bungkil kelapa, 20% pollard, dan 1% urea. Metode penelitian adalah eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 4 ulangan yaitu P1: Pucuk tebu 0% dan ampas tebu 100% yang difermentasi menggunakan jamur Phanerochaete chrysosporium, P2: Pucuk tebu 25% dan ampas tebu 75% yang difermentasi menggunakan jamur Phanerochaete chrysosporium, P3: Pucuk tebu 50% dan ampas tebu 50% yang difermentasi menggunakan jamur Phanerochaete chrysosporium, P4: Pucuk tebu 75% dan ampas tebu 25% yang difermentasi menggunakan jamur Phanerochaete chrysosporium, P5: Pucuk tebu 100% dan ampas tebu 0% yang difermentasi menggunakan jamur Phanerochaete chrysosporium. Data dianalisis menggunakan analisis variansi dan dilanjutkan uji Orthogonal Polinomial. Hasil penelitian menunjukan bahwa imbangan pucuk dan ampas tebu yang difermentasi menggunakan Phanerochaete chrysosporium berpengaruh secara linier terhadap konsentrasi VFA (P<0,01) dengan persamaan Y = -13x + 165 r2 = 0,75, tetapi tidak berpengaruh nyata terhadap konsentrasi NH3 dan sintesis protein mikroba (P>0,05). Kesimpulan dari penelitian adalah semakin tinggi taraf ampas tebu yang difermentasi menggunakan Phanerochaete chrysosporium 10 g/kg konsentrasi VFA semakin meningkat namun tidak memberikan makna terhadap konsentrasi NH3 dan sintesis protein mikroba, meskipun cenderung meningkat selaras dengan meningkatnya taraf ampas tebu serta penggunaan pucuk tebu dapat diganti sepenuhnya oleh ampas tebu.   Kata Kunci : Phanerochaete chrysosporium, Fermentasi, Konsentrasi VFA Total, NH3 serta Sintesis Protein Mikroba Rumen. A research entitled the ratio of cane top-bagasse fermented with Phanerochaete chrysosporium, it’s effect on rumen fermented product was conducted from December until March 2013. The materials used in this research were rumen fluid of beef cattle had been slaughtered and consisted of concentrate and the mixture ofcane top and bagasse in 60:40 ratio scale. The mixture was fermented with Phanerochaete chrysosporiumin 10 g/kg of cane top and bagasse mixture. The concentrate consist of 14% ground corn, 40% ricebran, 5% tapioca-by product, 20% coconut meal, 20% pollard and 1% urea. This research was conducted experimentally using Completely Randomized Design (CRD) with 5 treatments and 4 replicates. The treatments in this method were P1 = 0% cane top and 100% bagasse fermented with Phanerochaete chrysosporium, P2 = 25% cane top and 75% bagasse fermented with Phanerochaete chrysosporium, P3 = 50% cane top and 50% bagasse fermented with Phanerochaete chrysosporium, P4 = 75% cane top and 25% bagasse fermented with Phanerochaete chrysosporium, P5 = 100% cane top and 0% bagasse fermented with Phanerochaete chrysosporium. Data were analyzed using analysis of variance and continued by orthogonal polinomial test. The results showed that the fermented cane top and bagasse resulted in a linear highly significant effect on VFA concentration, Y = -13.76× + 165 r2 = 0.75, but showed no significant effect on NH3 and microbial protein synthesis. The conclusions of the research is the higher the level of bagasse using Phanerochaete chrysosporium10 g/kg of cane top and bagasse mixture, the higher total VFA concentration but not give meaning to the NH3 concentration and microbial protein synthesis, although it tended to increase in line with increasing levels of bagasse and cane tops usage can be replaced entirely by bagasse.   Keywords : Phanerochaete chrysosporium, Fermentation, Total VFA Concentration, NH3 and rumen microbial protein synthesis.

FERMENTASI AMPAS TEBU (Bagasse) MENGGUNAKAN Phanerochaete chrysosporium SEBAGAI UPAYA MENINGKATKAN KECERNAAN BAHAN KERING DAN KECERNAAN BAHAN ORGANIK SECARA in vitro

Jurnal Ilmiah Peternakan Vol 1, No 2 (2013): Jurnal Ilmiah Peternakan
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Penelitian bertujuan untuk mengkaji pengaruh taraf Phanerochaete chrysosporium pada fermentasi ampas tebu (Bagasse) terhadap tingkat kecernaan bahan kering dan kecernaan bahan organik pakan. Materi yang digunakan dalam penelitian adalah pakan yang terdiri dari ampas tebu dan konsentrat dengan imbangan 40:60, cairan rumen sapi dari sapi yang dipotong. Konsentrat tersusun dari 28% bungkil kelapa, 30% dedak halus, 1% garam dan 1% kapur (CaCO3). Penelitian disusun menggunakan 4 perlakuan dan 5 kali ulangan, menggunakan Rancangan Acak Lengkap (RAL). Pakan perlakuan adalah P0 : Pakan yang mengandung ampas tebu tanpa fermentasi (Phanerochaete chrysosporium 0 gram). P1, P2, P3 : pakan yang mengandung ampas tebu (bagasse) 5 g, 10 g, dan 15 g/kg BK ampas tebu. Parameter yang ukur dalam penelitian ini meliputi kecernaan bahan kering dan kecernaan bahan organik. Hasil penelitian menunjukkan bahwa fermentasi menggunakan Phanerochaete chrysosporium memberikan pengaruh nyata (P<0,05) terhadap kecernaan bahan kering secara linier dengan persamaan Y  = 0,6792X + 35.418, (r2= 0,96). Demikian pula terhadap kecernaan bahan organik juga berpengaruh secara linier dengan persamaan Y  = 0,746X + 24.422, (r2= 0,94). Kesimpulan dari penelitian yaitu sampai taraf 15% (15 g/kg BK)  fermentasi ampas tebu (bagasse) menggunakan Phanerochaete chrysosporium menunjukkan kecernaan bahan kering dan bahan organik pakan  masih meningkat. Kata kunci: Ampas tebu, fermentasi, Phanerochaete chrysosporium, kecernaan bahan kering (KBK) dan kecernaan bahan organik (KBO)   The objectives of the research were to investigate the influence of Phanerochaete chrysosporium against granting adequate level of dry matter digestibility (DMD) and organic matter digestibility (OMD) of feed based sugarcane (Bagasse). The materials used in this research were sugarcane bagasse and concentrate with ratio of 40 : 60, and rumen fluid of beef cattle that had been slaughtered. The concentrate was composed of  28% coconut meal, 20% rice bran, 1% salt and 1% lime stone (CaCO3). This research was conducted experimentally using Completly Random Design (CRD) with 4 treatments and  5 replicates. The treatments were P0: Feed contained sugarcane bagasse without fermentation, P1, P2, and P3:  Feed contained sugarcane bagasse of 5 g, 10 g, and 15 g of Phanerochaete chrysosporium (g/kg DM). The results of this research showed that the treatments (Phanerochaete chrysosporium fermentation) had significant  (P<0.05) effect on the digestibility of dry matter (DMD) and organic matter digestibility (OMD) were liniear with the equations Y  = 0,6792X + 35.418, (r2= 0.96) and Y  = 0,746X + 24.422, (r2= 0.94), respetivelly. The conclusion of this study was that the level of Phanerochaete chrysosporium of up to 15% (15 g/kg) in the fermentation sugarcane bagasse, the level digestibilities of dry matter (DMD) and organic matter (OMD) increased.   Keyword: sugarcane (bagasse), fermentation, Phanerochaete chrysosporium, dry matter digestibility (DMD) and organic matter digestibility (OMD)

PENGARUH IMBANGAN RUMPUT LAPANG – KONSENTRAT TERHADAP KECERNAAN BAHAN KERING DAN BAHAN ORGANIK SECARA IN VITRO

Jurnal Ilmiah Peternakan Vol 1, No 3 (2013): Jurnal Ilmiah Peternakan
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ABSTRAK Penelitian bertujuan untuk mengkaji kecernaan bahan kering dan kecernaan bahan organik pada pakan imbangan hijauan rumput lapang – konsentrat yang mengandung kulit nanas yang difermentasi dengan  Saccharomyces cereviseae.Penelitian dilaksanakan selama 1 bulan dari tanggal 13Januari 2013 sampai dengan 14 Februari 2013di Laboratorium Ilmu Bahan Makanan Ternak, Fakultas Peternakan Universitas Jenderal Soedirman, Purwokerto.Materiyang digunakan adalah pakan yang tersusun darirumput lapangdan konsentrat dengan perbandingan 30:70;50:50; 70:30. Cairan rumen sebagai sumber inokulum  diambil dari sapi yang dipotong di Rumah Potong Hewan(RPH). Metode penelitian yang digunakan adalah metode experimental secara in vitro yang dirancang menggunakan Rancangan Acak Lengkap (RAL). Perlakuan yang diuji adalah R1= Imbangan Hijauan Rumput Lapang-Konsentrat  30-70,R2 = Imbangan Hijauan Rumput Lapang-Konsentrat50-50,dan R3= Imbangan Hijauan Rumput Lapang-Konsentrat 70-30. Peubah yang diukur meliputi kecernaan Bahan Kering dan Bahan Organik . Data yang diperoleh dianalisis menggunakan analisis variansi, bila perlakuan berpengaruh nyata dilanjutkan dengan Uji Orthogonal Polynomial.Hasil analisis ragam menunjukkan bahwa imbangan hijauan rumput lapang – konsentrat berpengaruh nyata (P<0,05) terhadap kecernaan bahan kering akan tetapi tidak berpengaruh nyata terhadapkecernaan bahan organik pakan. Berdasarkan uji orthogonal polynomial perlakuan berpengaruh secara linier (P<0,05) terhadap kecernaan bahan kering dengan persamaan Y = 42,52+0,11 X dan koefisien determinasi (R2) 37,82 %. Berdasarkan hasil penelitian dapat disimpulkan bahwa pakan dengan imbangan hijauan rumput lapang : konsentrat sebesar 30:70  menghasilkan rataan kecernaan bahan kering dan  kecernaan bahan organik  tertinggi. Kata kunci :ratio Rumput lapang-Konsentrat, kecernaan bahan kering, kecernaan bahan organic,in-vitro ABSTRACT " Effect of ratio of wild Grass - Concentrate on digestibilities of  Dry Matter  and Organic Matter by In-Vitro". The research aimed to assess the digestibility of dry matter and organic matter in feed of wild grass - concentrate ratio containing fermented pineapple peel with Saccharomyces cereviseae. The research was carried out for 1 month from the date of January 13, 2013 until February 14, 2013 at the Feedstuff Science Laboratory, Faculty of Animal Science, Jendral Soedirman University, Purwokerto.The materials  used were feeds that were composed of wild grass and concentrate with a ratio of 30:70; 50:50; and 70:30. Rumen fluid as a source of inoculum was taken from cattle abattoar (slaughterhouse). The research method was experimental by in-vitro methods,that was designed using completely randomized design (CRD). The treatments tested were R1 =  wild Grass –Concentrate ratio of  30:70, R2 = willd Grass –Concentrate of  50:50,and R3 = wild Grass–Concentrate of 70:30. The  measured variables included digestibilities of Dry Matter  and Organic Matter. The data were analyzed using analysis of variance.  If the  treatments significantly affected the variables being  tested, the test was continued with Orthogonal polynomial.The results of analysis of variance showed that the ratio of wild  grass - concentrate significantly (P <0.05) affected the digestibility of dry matter, but had no significant effect on organic matter digestibility of feed. Based on orthogonal polynomial test,there was a linear  effect (P <0.05) of  trertment on the digestibility of dry matter with the equation, Y = 42.52 +0.11 X and the coefficient of determination (R2) of 37.82%. Based on the results of this study,it was concluded that the ratio of wild  grass to concentrate  at 30:70  produced thehighest dry matter digestibility and organic matter digestibility. Keywords:ratio wild grass-concentrate, dry matter digestibility, organic matter digestibility,in-vitro

PENGARUH LAMA PEMERAMAN TERHADAP KANDUNGAN LEMAK KASAR DAN SERAT KASAR SILASE COMPLETE FEED LIMBAH RAMI

Jurnal Ilmiah Peternakan Vol 2, No 1 (2014): Jurnal Ilmiah Peternakan (JIP)
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ABSTRAK Tujuan penelitian adalah untuk mengetahui pengaruh lama pemeraman pada silase complete feed limbah rami dengan penggunaan sumber protein nabati dan protein hewani  terhadap kandungan  lemak kasar dan serat kasar. Materi yang digunakan dalam penelitian adalah complete feed yang tersusun atas limbah daun dan batang rami, gaplek, bekatul, onggok, molases, urea, garam, kapur, dan mineral mix serta tepung ikan (sumber protein hewani), menir dan bungkil kedelai (sumber protein nabati).  Metode yang digunakan adalah metode eksperimental dengan Rancangan Acak Lengkap (RAL) dengan 8 perlakuan yang diulang sebanyak 4 kali.Variabel yang diukur adalah kandungan lemak kasar dan serat kasar. Hasil analisis variansi menunjukan bahwa perlakuan lama pemeraman silase complete feed limbah berpengaruh nyata P < 0,05 terhadap kandungan lemak kasar, sedangkan serat kasar tidak berpengaruh nyata. Rataan kandungan lemak kasar perlakuan CF1N0, CF1N1, CF1N2, CF1N3 dan CF1H0, CF1H1, CF1H2, CF1H3 masing – masing adalah 3,71%; 3,06%; 3,28%; 2,56% dan 3,88%; 2,96%; 3,51%; 2,60%. Rataan kandungan serat kasar perlakuan sumber protein (nabati) CF1N0, CF1N1, CF1N2, CF1N3 dan (hewani) CF1H0, CF1H1, CF1H2, CF1H3 adalah 13,39%; 13,46%; 14,58%; 15,30% dan 14,71%; 14,83%; 14,36%; 14,63%. Disimpulkan bahwa tidak terjadi perubahan kandungan serat kasar pada complete feed yang dibuat silase dengan sumber protein yang berasal dari nabati maupun hewani. Namun terjadi perubahan kandungan lemak kasar yang semakin menurun dengan semakin lama diperam. Kata Kunci : Silase, lama pemeraman, limbah rami, lemak kasar dan serat kasar ABSTRACT The aim of the research was to know the effect of long storage on vegetable source of protein or animal source of protein the contents of ether extract and crude fiber of complete feed silage of jute waste.  The materials in the research consisted of complete feeds composed ofhemp leaves and stems, gaplek, rice bran, tapioca by product, molasses, urea, salt, lime stone, and mineral mix and fish meal (source of animal protein), soya groats and soybean meal (source of vegetable protein). The method used was an experimental method with a completely randomized design (RAL), with 8 treatments consisted of 4 repetition. The measured variable were the contents of crude fat and crude fiber. The results of analysis of variance showed that the treatment of storage significantly (P <0.05) affected for ether extract content, but had no significant on crude fiber content. The increasing average values of ether extract contents for  CF1N0,  CF1N1, CF1N2, CF1N3 and CF1H0, CF1H1, CF1H2, CF1H3 respectively were 3.71%, 3.06%, 3.28%, 2.56% and 3.88%; 2.96%, 3.51%, 2.60%. The increasing average values of crude fiber contents of protein sources (vegetable); CF1N0, CF1N1, CF1N2, CF1N3 and (animal); CF1H0, CF1H1, CF1H2, CF1H3 were 13.39%, 13.46%, 14.58%, 15.30% and 14 , 71%, 14.83%, 14.36%, 14.63%. It concluded that no change in crude fiber content in complete feed silage supplemented ​​with protein source derived from vegetable and animal. However, a change in crude fat content decreases with the longer storage. Keyword: Silage, storage , jute waste,  ether extract and crude fiber

The Improvement of Rumen Fermentation Products Through In-Vitro Supplementation of Mg and Co Minerals

ANIMAL PRODUCTION Vol 18, No 2 (2016): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

A study has been conducted to examine the effect of Mg and Co supplementation on rumen fermentation products. The study was conducted in an in vitro experiment, applying completely randomized design, 3x3 factorial. The first factor was three levels of Mg (0; 7.5 and 15.0 mM), and three levels of Co (0; 0.03 and 0.06 mM) as the second factor, total 9 treatments each repeated three times, comprising 27 experimental units all together. In vitro incubation lasted for 4 hours. Variables measured were the concentrations of VFA, N-NH3 and protein synthesis of microbial rumen. Data were subject to analysis of variance and orthogonal polynomials test. The results showed an interaction effect between Mg and Co on the concentration of VFA, N-NH3 and protein synthesis of microbial rumen. The increasing supplementation of Mg at 0.06 mM Co increased VFA concentration; the highest concentration of N-NH3 was achieved by rumen fluid supplemented with 15.0 mM of Mg and 0.03 mM of Co. The highest protein synthesis of microbial rumen was achieved by the rumen fluid supplemented with 8.18 mM of Mg with no supplementation of Co.

The Effects of Branched Chain Volatile Fatty Acids on Reduced Sugar and Branched Chain Amino Acid Concentration of Substrates that Fermented by Aspergillus oryzae

ANIMAL PRODUCTION Vol 4, No 2 (2002): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

A study was conducted to know the reduced sugar and branched chain amino acids concentration in substrate that fermented by Aspergillus oryzae. Branched chain amino acids represent amino acids that are very important for microorganism development, including yeast and ruminal microorganism as well as for the growth of the ruminant animal. The study was conducted using Completely Randomized Design (CRD). There were five kinds of supplements that were added into the media. So, that this experiment were A: control, B: A + 0.5% urea, C: B + 1% extract of cassava leaves, D: C + 1% isobutyrate, and E: D + 1.3% 2-methilbutyrate. There were five replicates in each treatment. The measured variables in these study were, colonies cell biomass of A. oryzae, reduced sugar, Crude Protein, and branched chain amino acid concentration. The results showed that the highest number of colonies, concentration of reduce sugar, and concentration of branched chain amino acids was obtain from the substrate of treatments D. (Animal Production 4(2): 83-88 (2002) Key words : Branched Chain Amino Acids, Branched Chain Volatile Fatty Acids, Aspergillus oryzae

Fermentasi Jerami Padi Menggunakan White rot fungi dan Suplementasi Saccharomyces cerevisiae Pengaruhnya terhadap Kecernaan Nutrien Secara In Vitro

Jurnal Agripet Vol 12, No 2 (2012): Volume 12, No. 2, Oktober 2012
Publisher : Agricultural Faculty

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Abstract

The influence of rice straw fermentation using white rot fungi and saccharomyces cerevisiae supplementation on in vitro nutrient digestibilityABSTRACT. An experiment to investigate the effect of rice straw fermented using white rot fungi and Saccharomyces cerevisiae supplementation on nutrient digestibility In Vitro had been implemented in two phases. The first experiments undertaken to make rice straw fermentation, using experimental methods with a Completely Randomized Design. As the treatment were White rot fungi (Phanerochaete chrysosporium) 0, 5 and 10 g/kg of rice straw (DM basis). Each treatment was repeated six times, so there are 18 experimental units. The variables measured included nutrient content of rice straw. A second experiment carried out in vitro to test the best rice straw fermentation results of the first experiment, using experimental methods, with a Completely Randomized Design. As the treatment were the supplementation of Saccharomyces cerevisiae (0, 2, 4% of the weight of fermented rice straw, DM basis). The variables measured included digestibility of dry matter, organic matter, cellulose and lignin digestibility of feed containing fermented straw. The data obtained were analyzed using analysis of variance test followed by Orthogonal Polynomials. The results can be concluded that the fermented rice straw using Phanerochaete chrysosporium 10 g/kg of rice straw is the best nutrient content. Saccharomyces cerevisiae supplementation on feed that contains fermented rice straw using Phanerochaete chrysosporium 10 g/kg rice straw is 2%.