Wardhana Suryapratama
Fakultas Peternakan Universitas Jenderal Soedirman

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The Improvement of Rumen Fermentation Products Through In-Vitro Supplementation of Mg and Co Minerals Suhartati, FM; Suryapratama, Wardhana
ANIMAL PRODUCTION Vol 18, No 2 (2016): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (772.012 KB) | DOI: 10.20884/1.anprod.2016.18.2.552

Abstract

A study has been conducted to examine the effect of Mg and Co supplementation on rumen fermentation products. The study was conducted in an in vitro experiment, applying completely randomized design, 3x3 factorial. The first factor was three levels of Mg (0; 7.5 and 15.0 mM), and three levels of Co (0; 0.03 and 0.06 mM) as the second factor, total 9 treatments each repeated three times, comprising 27 experimental units all together. In vitro incubation lasted for 4 hours. Variables measured were the concentrations of VFA, N-NH3 and protein synthesis of microbial rumen. Data were subject to analysis of variance and orthogonal polynomials test. The results showed an interaction effect between Mg and Co on the concentration of VFA, N-NH3 and protein synthesis of microbial rumen. The increasing supplementation of Mg at 0.06 mM Co increased VFA concentration; the highest concentration of N-NH3 was achieved by rumen fluid supplemented with 15.0 mM of Mg and 0.03 mM of Co. The highest protein synthesis of microbial rumen was achieved by the rumen fluid supplemented with 8.18 mM of Mg with no supplementation of Co.
The Effects of Branched Chain Volatile Fatty Acids on Reduced Sugar and Branched Chain Amino Acid Concentration of Substrates that Fermented by Aspergillus oryzae Suhartati, FM; Suryapratama, Wardhana
ANIMAL PRODUCTION Vol 4, No 2 (2002): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (108.188 KB)

Abstract

A study was conducted to know the reduced sugar and branched chain amino acids concentration in substrate that fermented by Aspergillus oryzae. Branched chain amino acids represent amino acids that are very important for microorganism development, including yeast and ruminal microorganism as well as for the growth of the ruminant animal. The study was conducted using Completely Randomized Design (CRD). There were five kinds of supplements that were added into the media. So, that this experiment were A: control, B: A + 0.5% urea, C: B + 1% extract of cassava leaves, D: C + 1% isobutyrate, and E: D + 1.3% 2-methilbutyrate. There were five replicates in each treatment. The measured variables in these study were, colonies cell biomass of A. oryzae, reduced sugar, Crude Protein, and branched chain amino acid concentration. The results showed that the highest number of colonies, concentration of reduce sugar, and concentration of branched chain amino acids was obtain from the substrate of treatments D. (Animal Production 4(2): 83-88 (2002) Key words : Branched Chain Amino Acids, Branched Chain Volatile Fatty Acids, Aspergillus oryzae
Fermentasi Jerami Padi Menggunakan White rot fungi dan Suplementasi Saccharomyces cerevisiae Pengaruhnya terhadap Kecernaan Nutrien Secara In Vitro Suryapratama, Wardhana; Suhartati, Fransisca Maria
Jurnal Agripet Vol 12, No 2 (2012): Volume 12, No. 2, Oktober 2012
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.744 KB) | DOI: 10.17969/agripet.v12i2.195

Abstract

The influence of rice straw fermentation using white rot fungi and saccharomyces cerevisiae supplementation on in vitro nutrient digestibilityABSTRACT. An experiment to investigate the effect of rice straw fermented using white rot fungi and Saccharomyces cerevisiae supplementation on nutrient digestibility In Vitro had been implemented in two phases. The first experiments undertaken to make rice straw fermentation, using experimental methods with a Completely Randomized Design. As the treatment were White rot fungi (Phanerochaete chrysosporium) 0, 5 and 10 g/kg of rice straw (DM basis). Each treatment was repeated six times, so there are 18 experimental units. The variables measured included nutrient content of rice straw. A second experiment carried out in vitro to test the best rice straw fermentation results of the first experiment, using experimental methods, with a Completely Randomized Design. As the treatment were the supplementation of Saccharomyces cerevisiae (0, 2, 4% of the weight of fermented rice straw, DM basis). The variables measured included digestibility of dry matter, organic matter, cellulose and lignin digestibility of feed containing fermented straw. The data obtained were analyzed using analysis of variance test followed by Orthogonal Polynomials. The results can be concluded that the fermented rice straw using Phanerochaete chrysosporium 10 g/kg of rice straw is the best nutrient content. Saccharomyces cerevisiae supplementation on feed that contains fermented rice straw using Phanerochaete chrysosporium 10 g/kg rice straw is 2%.
Kualitas Karkas Domba Lokal yang Diberi Pakan Jerami Padi Fermentasi dengan Suplementasi Minyak Kedelai Patriani, Peni; Sumarmono, Juni; Suryapratama, Wardhana
Jurnal Agripet Vol 10, No 2 (2010): Volume 10, No. 2, Oktober 2010
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.002 KB) | DOI: 10.17969/agripet.v10i2.645

Abstract

Carcass quality of local sheep fed fermented rice straws supplemented with soybean oilABSTRACT. The aim of this study was to determine if supplementation of soybean oil in the ration in order to increase conjugated linoleic acid of meat has concurrent effects on carcass characteristics and qualities of thin-tailed sheep. Sheep were fed fermented ricestraws supplemented with 0, 3 and 6% soybean oil. The experimental design used was a Randomized Completely Block Design. Eighteen sheep with initial age of 6-8 months and initial bodyweight of 17.46 ± 1.61 kg were used. The treatments consisted of soybean oil supplementation 0, 3 and 6%. Variables measured were carcass conformation, GR thickness, backfat thickness, subcutaneous fat score, and kidney and pelvic fat score. Result showed that sheep fed ration with 0 % soybean oil produced carcass with carcass conformation score of 8.50 ± 1.05, GR thickness 11.50 ± 1.05 mm, back fat thickness 2.67 ± 0.82 mm, subcutaneus fat score 3.50 ± 0.55 mm, kidney and pelvic fat score 2.00 ± 0.00. Sheep feed ration with 3% soybean oil; carcass conformation score 8.67 ± 1.21, GR thickness 11.67 ± 1.21 mm, back fat thickness 2.83 ± 0.98 mm, subcutaneus fat score 3.50 ± 0.55, kidney and pelvic fat score 1.83 ± 0.4. Sheep feed ration with 6% soybean oil; carcass conformation score 9.00 ± 1.26, GR thickness 12.50 ± 1.52 mm, back fat thickness 3.33 ± 0.82 mm, subcutaneus fat score 3.50 ± 0.55, kidney and pelvic fat score 1.83 ± 0.41. Score of carcass conformation, thickness of GR, thickness of back fat, score of subcutaneus fat, and score of kidney-pelvic fat were not influenced by supplementation of soybean oil. In conclusion, suplementation soybean oil in the ration to increase conjugetaed linoleic acid has no significant concurrent effect on carcass quality characteristics of local sheep.
PENGARUH DAUN TURI (Sesbania grandiflora) DAN LAMTORO (Leucaena leucocephala) DALAM RANSUM SAPI BERBASIS INDEKS SINKRONISASI PROTEIN - ENERGI TERHADAP SINTESIS PROTEIN MIKROBA RUMEN Nurus Syamsi, Afduha; Maria Suhartati, Fransisca; Suryapratama, Wardhana
Pastura : Jurnal Ilmu Tumbuhan Pakan Ternak Vol 6 No 2 (2017): Pastura Vol. 6 No. 2 Tahun 2017
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.24843/Pastura.2017.v06.i02.p01

Abstract

An experiment was aimed to assess the use of the legume leaf as a source of protein feedstuff and levelsof synchronization protein-energy (SPE) index in the diet of cattles on ammonia (N-NH3) and microbialprotein synthesis (MPS). In vitro techniques was done. The research was used a completely randomizeddesign (CRD), with factorially pattern (2x3), the first factor was the two species of legume (Sesbania leavesand Leucaena leaves) and the second factor was the three level of the SPE index (0.4, 0.5, and 0.6), therewere 6 treatment combinations and each was 4 replicates. The results showed that no interaction betweenlegume with SPE index, but each factor was significantly effect (P<0.05) on N-NH3 of rumen fluid andMPS. The research concluded that Leucaena leaf is a legume that is better than Sesbania leaf in terms oftheir ability to increase MPS. SPE index is the best in producing MPS at level 0.6.Key words: Legume, synchronization of protein and energy index, ammonia, microbial protein synthesis