Articles

Control of Fusarium Wilt of Chili With Chitinolytic Bacteria Suryanto, Dwi; Patotah, Siti; Munir, Erman
HAYATI Journal of Biosciences Vol 17, No 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.17.1.5

Abstract

Biological control of plant disease using antagonistic microorganism has been obtaining much attention and implemented for decades. One of the potential microorganisms used in this strategy is chitinolytic bacteria. Utilization of this bacteria ranges from cell life, enzymes, genes, or other metabolites. In this study, we examined the ability of chitinolytic bacteria as a biocontrol agent of Fusarium wilt of red chili (Capsicum annuum L.) seedlings. The ability of chitinolytic bacteria to suppress the disease was evaluated by soaking red chili seeds in the bacterial isolates solution for 30 minutes prior seedling. Percentage of seedling of treated chili seed at end of study (4-weeks) ranging from 46 to 82.14%. Relative reduction of the seedling damping-off was observed in all bacterial treatment ranged from 28.57 to 60.71%. Furthermore, manifestation of bacterial suppression to Fusarium wilt was also exhibited by increasing of seedling height (ranged from 7.33 to 7.87 cm compared to 6.88 cm) and dry-weight (ranged from 2.7 to 4.3 mg compared to 2.3 mg). However, no significant effect was observed in leaf number. Then, from all chitinolytic isolates tested, BK08 was the most potential candidate for biological control agent of Fusarium wilt in chili seedling.
Antimicrobial Activity of Endemic Herbs from Tangkahan Conservation Forest North Sumatera to Bacteria and Yeast NURTJAHJA, KIKI; KELANA, TATA BINTARA; SURYANTO, DWI; PRIYANI, NUNUK; RIO, GINTA; PUTRA, DEDI PRIMA; ARBAIN, DAYAR
HAYATI Journal of Biosciences Vol 20, No 4 (2013): December 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.20.4.177-181

Abstract

Tangkahan Conservation Forest in Karo County, North Sumatera has high biodiversity of endemic herbs. Many species of the wild herbs are well known used as traditional medicine not only by local people but also by people out of the area. The methanol extract of the medicinal wild herbs in Tangkahan Conservation Forest, Karo County to relief skin diseases caused by bacteria and fungi never been studied medically. The antimicrobial activity leave extract of the medicinal herbs to pathogenic  microorganisms are studied. The leaves extract of kembu-kembu (Callicarpa candicans), rintih bulung (Piper muricatum), cep-cepan (Castanopsis costata), and sereh kayu (Eugenia grandis), has antimicrobial to bacteria (Bacillus sp., Escherichia coli, Serratia marcescens, Staphylococcus aureus) and yeast (Candida albicans). Toxicity assay of these plants by brine shrimp method using Artemia salina indicates that cep-cepan dan sereh kayu have lethal concentration higher than kembu-kembu and rintih bulung.
PEMBERDAYAAN MASYARAKAT BERBASIS PENDEKATAN KELUARGA DALAM UPAYA PENINGKATAN KESEJAHTERAAN HIDUP DI KELURAHAN PAMOYANAN, BOGOR SELATAN TAHUN 2018 Prastia, Tika Noor; Suryanto, Dwi; Ilmi, Hana; Safitri, Rezki Ayu
PROMOTOR Vol 1, No 2 (2018)
Publisher : Fakultas Ilmu Kesehatan, Universitas Ibn Khaldun, Bogor

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Abstract

Pemberdayaan masyarakat merupakan sebuah metode dalam upaya membangun kemandirian masyarakat untuk meningkatkan kesejahteraan hidup. Menciptakan kemandirian masyarakat dimulai dari lingkup keluarga. Tujuan program adalah untuk menumbuhkan kemandirian masyarakat secaraholistik baik lingkungan, ekonomi, dan kesehatan. Sasaran program adalah warga RW 3 dan RW 12 di keluarahan Pamoyanan Bogor Selatan. Program yang dilakukan meliputi penyuluhan dan cek kesehatan, pembuatan hidroponik, dan pengembangan produk ekonomi lokal. Metode yangdigunakan meliputi ceramah dan diskusi (penyuluhan dan cek kesehatan), tutorial (pembuatan hidroponik), dan pengembangan media pengembangan produk ekonomi lokal). Pengolahan data menggunakan analisis univariat untuk mendeskripsikan hasil program. Hasil program menunjukkanbahwa persentase penderita hipertensi 26,3%, penderita diabetes 28,9%, sebagian besar (55,5%) dari peserta yang hadir telah menanam hidroponik, dan peningkatan penjualan produk > 100% dari penjualan awal. Program-program yang dilaksanakan memiliki dampak positif bagi masyarakatdengan terdeteksinya penyakit degeneratif, kemampuan budidaya tanaman dengan metode hidroponik, dan peningkatan produk ekonomi lokal melalui pengembangan media.
Selection and Isolation of Aromatic Hydrocarbon Degrading Bacteria Suryanto, Dwi; Suwanto, Antonius
Jurnal Mikrobiologi Indonesia Vol 5, No 2 (2000): JURNAL MIKROBIOLOGI INDONESIA
Publisher : Jurnal Mikrobiologi Indonesia

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Abstract

Thirthy-four anoxygenic photosynthetic bacteria and 7 other environmental isolates were examined for theirability to grow anaerobically in light in monocyclic aromatic compound, including beuzoate, salysilate 2-bydroxybenzoate, phenol, gentisate 2,5-dihydroxybenzoate, and catechol 1,2-benzenediol. Five aerobic bacteriawere tested for aerobic utilization ot´ the monocyclic aromatic compound. Twenty-seven Out of 41 of isolated anoxygenicphotosynthetic bacteria 65.8% were able to grow in 5 mM benzoate. DS-1, DS-4, and Cas-13 of the anoxygenicphotosynthetic bacteria and DS-8, MR1.2, and PG9.1 of the aerobic bacteria showed relatively short generation time.The ability of purple non-sulfur bacteria to grow In gentisate might be firstly reported. We also observed that MR1.2was the only Gram positive isolate of the aerobic bacteria capable of growing In atrazine 2-chloro-4-ethylamlno-6-isopropylamino.l,3,5.triazlne.
VIABILITAS DAN KERIAP Bacillus sp. BK17 DAN Enterobacter sp. BK15 PADA SUMBER KARBON DAN NITROGEN YANG BERBEDA Nasrah, Sirma Novita; Suryanto, Dwi; Jamilah, It
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

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Abstract

To propagate bacterial cell for biocontrol purpose, suitable nutrient have to be determined in which carbon and nitrogen source was often as limited factor of bacterial growth. Proper storage for biocontrol agent such as bacterial cell should also be considered in order to keep the cell viable when used. The aim of this study is to find out suitable carbon and nitrogen sources for viability and swarming of chitinolitic bacterial Bacillus sp. BK17 and Enterobacter sp. BK15. The highest population of bacterial growth (3.7x108 cfu/ml) was found in molases-sodium nitrate (MS) medium and the lowest population was found in crab shell-sodium nitrate (CS) growth (2.4x108 cfu/ml) after 25 days of incubation. The swarming activity of the isolates were varied to some extent with the highest was 51 mm in 2% agar molases-urea after 5 days of incubation. Molases-sodium nitrate (MS) medium is suitable carbon and nitrogen source for the viability of Bacillus sp. and Enterobacter sp. Meanwhile, agar molases-urea medium with 2% agar is suitable medium for swarming ability for both bacteria. Keywords : Bacillus, C and N-source, Enterobacter, swarming, viability
UJI POTENSI BAKTERI KITINOLITIK DALAM MENGHAMBAT PERTUMBUHAN Rhizoctonia solani PENYEBAB REBAH KECAMBAH PADA KENTANG VARIETAS GRANOLA Novina, Dewi; Suryanto, Dwi; D, Elimasni
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

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Abstract

A study on assay the potential of chitinolytic bacterial isolates to inhibit the growth of Rhizoctonia solani causal agent of damping-off on potato was carried out  in Laboratory of Observation Pest and Disease, Medan Johor, UPT. Protection of Crops and Holticulture 1 and Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Science of Universitas Sumatera Utara, Medan. Two out of six isolates of chitinolytic bacteria showed capabilities to inhibit of R. solani growth. The most effective isolates to inhibit the growth of R. solani were Enterobacter sp. BK15 and Bacillus sp. BK13, that were able with inhibition zone of 2.05 and 2.35 cm, respectively. These two isolates also showed to be able to reduce fungal infection by 31.25% and 37.5%, respectively.   Keywords: Bacillus sp. BK13, damping-off, Enterobacter sp. BK15, R. solani, potato
PEMANFAATAN BAKTERI KITINOLITIK DALAM MENGHAMBAT PERTUMBUHAN Curvularia sp. PENYEBAB PENYAKIT BERCAK DAUN PADA TANAMAN MENTIMUN Hanif, Andini -; Suryanto, Dwi; Nurwahyuni, Isnaini
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

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Abstract

A study on about the utilization of chitinolytic bacterial isolates to inhibit growth of Curvularia sp. causal agent of leaf spot disease of cucumber was done in Laboratory of Pest and Disease, Medan Johor, UPT-Balai Protection Plant and Horticulture I and Laboratory Microbiology Departement of Biology, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Medan. Six chitinolytic isolates were tested in vitro to inhibit growth of Curvularia sp.. The result showed that Bacillus sp. BK13 inhibited more with inhibition zone of 2,75 cm and Enterobacter sp. BK15 with inhibition zone of 2,55 cm, whereas Enterobacter sp. PB17 showed the lowest inhibition zone of 1,3 cm. Soaking seed treatment in chitinolytic bacterial suspension reduced the percentage of leaf spot. Enterobacter sp. BK15 showed more ability (50%) to inhibit leaf spot attack.   Keywords: Bacillus sp. BK13, cucumber, Curvularia sp., Enterobacter sp. BK15, leaf spot
PENGENDALIAN SEL BIOFILM BAKTERI PATOGEN OPORTUNISTIK DENGAN PANAS DAN KLORIN Silitonga, Yusnita Wahyuni; Jamilah, It; Suryanto, Dwi
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

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Abstract

Opportunistic pathogenic bacteria are bacteria that are naturally not present in an environment that, but it dues to contamination of the environment by human waste. From previous research it was found E. coli, Staphylococcus sp. and Salmonella were opportunistic pathogens of the shrimp aquaculture (Percut, Pantai Labu, Pantai Cermin). The aims of this study is to know the ability of these bacteria to form biofilms as well as its control using chlorine and heat. In order to test the ability of the bacteria to form biofilms, the stainless steel have been soaked in SWC media for 1, 3, 6 days. E. coli, Salmonella, and Staphylococcus sp. may form biofilms on incubation of the 1st day but the highest growth was on the 6th day of incubation by E. coli with a 6,35 x 104 CFU/SS, whereas of the lowest biofilm number was found in Salmonella with a 0,28 x 104 CFU/SS. The number of biofilm cells grow in line with a length of incubation. In this research, the most effective concentration of chlorine to kill biofilm cell was  225 ppm for 2 minutes and heat was 100 0C for 5 minutes. The higher the concentration of chlorine and the temperature given more effective to kill the bacteria.   Keyword: biofilm, chlorine, heat, opportunistic, stainless steel
PENGHAMBATAN SERANGAN Sclerotium rolfsii PENYEBAB REBAH KECAMBAH PADA KEDELAI DENGAN BAKTERI KITINOLITIK Malinda, Novi; Suryanto, Dwi; Nurtjahja, Kiki
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

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The inhibition of Sclerotium rolfsii as causal agent of soybean damping off by using chitinolytic bacteria were studied in Laboratory of Observation Pest and Disease UPT-BPTPH 1 and Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Medan. The aim of this research was to investigate  the ability of chitinolytic bacteria to inhibit the growth of S. rolfsii. Six isolates of chitinolytic bacteria were used in this study. The inhibition zone between colony of the bacteria and the fungus was measured. The result showed that the bacterial isolates has different ability in inhibiting the growth of S. rolfsii. Enterobacter sp. BK15 and Bacillus sp. BK13 were the most effective in inhibiting the growth of S. rolfsii with inhibition zone of 3.70 cm and 3.75 cm, respectively. The less effective was shown by Enterobacter sp. PB17 with inhibition zone of 0.10 cm, while Enterobacter cloacae LK08 have no ability to inhibit S. rolfsii. The seeds soaked in bacterial suspension by  Bacillus sp. BK13 dan Enterobacter sp. BK15 were able to reduce damping off by 44.4% and 50%, respectively.   Keywords: Bacillus sp., damping off, Enterobacter sp., Sclerotium rolfsii, soybean
POTENSI BAKTERI KITINOLITIK DALAM PENGENDALIAN Aspergillus niger PENYEBAB PENYAKIT BUSUK PANGKAL AKAR PADA TANAMAN KACANG TANAH Ayu, Arifda; Suryanto, Dwi; Nurwahyuni, Isnaini
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

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Abstract

A study of chitinolytic bacteria ability to control Aspergillus niger, a causal agent of basal root rot of peanut seedlings was conducted in Pest and Disease Laboratory, Medan Johor and Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Medan.  The purpose of study is to evaluate the ability of Bacillus sp. BK13, Enterobacter sp. BK15, Bacillus sp. BK17, Enterobacter cloacae LK08, Bacillus sp. KR05, and Enterobacter sp. PB17 to inhibit the growth of A. niger on the peanut seedling.  Antagonistic test showed that the most effective bacteria in inhibiting the growth of A. niger was BK15 isolate with inhibition zone of 2,88 cm and BK13 isolate with inhibition zone of 2,69 cm, whereas the least effective bacteria was BK17, with inhibition zone of 2,30 cm. Chitinolytic bacterial isolates used to cover peanut seed through soaking enabled to reduce seed basal root rot. BK15 isolate showed potential to reduce the basal root rot by 58,82% while the lowest inhibition was BK13 by 47,06%.   Keywords: Aspergillus niger, Bacillus sp., chitinolytic bacteria, Enterobacter sp., peanut