Yadi Suryadi
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar No. 3A, Bogor 16111 Telp. (0251) 8337975

Published : 13 Documents

Found 13 Documents

Space Restraint Study to Establish Sustainable Bogor Municipality Suryadi, Yadi; Sutjahjo, Surjono H.; Sanim, Bunasor; Rustiadi, Ernan; Soma, Soekmana
Forum Pasca Sarjana Vol 31, No 4 (2008): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)


The change of environmental problem is very complex.  The highest population growth caused supply of land and natural resources to be increased.  If it does not control, it can influence natural resources stock and disturbs environmental balance system land use control is part of Bogor land use planning.  The objectives of this research were (1) to analyze the dynamics of land use and population in the period of 1972-2005 in Bogor Municipality, (2) to develop dynamics model and analyze the relation between the instrument of land use control and the factors related to the sustaina tozbility of Bogor Municipality, and (3) to analyze stakeholders’ roles benefited to space control system in Bogor Municipality.  The methods used for this research were (1) GIS analysis to observe the alteration influence landcover in period 1972-2005, (2) dynamic system analysis for landcover using in Bogor Municipality, and (3) AHP analysis to observe stakeholders’ role controlling use of landcover in Bogor Municipality.  This research indicate that the dynamic of landcover such as forestry, mixed plantation, and open area (1972, 1983 and 1990) before to be extended were larger than after.  For the landcover change such as resettlement, bush, water and wetland showed that were larger than before extended (2000 and 2005).  The population dynamic analysis showed the optimum increasing on 1985-2020 (22,38%) but was not followed by good landcover use planning as indicated by forestry depletion from 25% to 2%, depletion of mixed plantation from 42% to 36%, resettlement need increased from 12% to 43%, and declining open area from 17% to 2%.  These showed that Bogor developments were unsustainable.  The dynamic analysis showed the correlation between the environmental component and land use control.  Land use control to achieve Bogor sustainable development need many aspect such as land area, population number waste number, vehicle number, the length of roads and the role of stakeholder on land use control system in Bogor municipality though license mechanism and control.  The government can influence land use control at level of controlling and level of licensing.  For the government input as the authority that gives the permission of land using need to be consistent on the regulation they made.   Key words: the dynamic, land use model, and controlling
Analisis Keragaman Genetik Isolat Bakteri Xanthomonas oryzae pv. oryzae dari Jawa Barat dan Jawa Tengah Berdasarkan Analisis ARDRA Gen 16SrRNA Suryadi, Yadi; Susilowati, Dwi N.; Lestari, Puji; Sutoro, Sutoro; Manzila, Ifa; Kadir, Trini Suryani; Albani, Sheryn Sunni; Artika, I Made
Jurnal Fitopatologi Indonesia Vol 10, No 2 (2014)
Publisher : Jurnal Fitopatologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1104.442 KB)


Fifteen Xanthomonas oryzae pv. oryzae isolates from several regions in West Java and Central Java have been studied for their genetic diversity using amplified ribosomal DNA restriction analysis (ARDRA) 16SrRNA gene.Total of 13 ARDRA patterns from digestion of DNA with RsaI restriction enzyme was obtained, indicated higher genetic diversity of X. oryzae pv. oryzae. Phylogenetic analysis revealed a close genetic relationships among isolates. Isolates X. oryzae pv. oryzae 1/96pml and 61pml are closely related with isolates 29d, 59pml, and 60pml although they are isolated from different locations. The other closely related isolates were between X. oryzae pv. oryzae 5mgl with 23d, 38d, 10sbg, 8myd; while 6klt was close to 3ind and 2kr. The result of X. oryzae pv. oryzae genetic diversity can be used as a reference for strain or/isolates selection for development ofbacterial leaf blight resistance in Indonesia.  
Kajian Keragaman Genetik Isolat Ralstonia solanacearum BIOVAR 3 menggunakan Penanda REP-PCR Suryadi, Yadi
Jurnal Perlindungan Tanaman Indonesia Vol 5, No 1 (1999)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.9957


Study of DNA fingerprinting of genomic DNA of Australian Ralstonia solanacearum biovar 3 was characterized by a DNA BOX primer that correspond with repetitive sequence using PCR amplification (rep-PCR). Based on rep-PCR DNA profiles different band mobility were observed among Australian biovar 3. Most of isolates have shown common DNA amplification product at 600 bp. Cluster analysis to the DNA profiles showed two different DNA banding patterns that correlated with geographical origins of the isolates. Subgroup A correspond well with isolates from South Queensland/New South Wales, whilst subgroup B correspond with isolates from North Queensland origin.Key words: Ralstonia solanacearum, rep-PCR, marker
Identifikasi Entomopatogen Bakteri Merah pada Wereng Batang Coklat (Nilaparvata lugens Stål.) Priyatno, Tri Puji; Dahliani, Yohana A; Suryadi, Yadi; Samudra, I Made; Susilowati, Dwi Ningsih; Rusmana, Iman; Wibowo, Baskoro S; Irwan, Cahyadi
Jurnal AgroBiogen Vol 7, No 2 (2011): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (229.909 KB)


Indentification of Entomopathogenic Red Bacterial fromBrown Planthopper (Nilaparvata lugens Stål.). Tri P.Priyatno, Yohana A. Dahliani, Yadi Suryadi, I MadeSamudra, Dwi N. Susilowati, Iman Rusmana, Baskoro S.Wibowo, and Cahyadi Irwan. Red bacteria isolated frombrown planthopper (BPH) has been proven pathogenicagainst BPH and others insects. Application of 106 to 107cells/ml of red bacteria caused 65.6-78.2% mortality of BPH.The 50% effective concentration (EC50) and lethal time of redbacteria against BPH is 2.8 x 105 cells/ml and 6.8 days,respectively. Based on phenotypic characters tested on GNMicroPlateTM Biolog kit and 16S rRNA sequneces analysis,red bacteria was identified as Serratia marcescens with 99%similarity. Red pigmen produced by S. marcescens strainBPH is secondary metabolite determined as prodigiosinshowing bactericidal activities against Xanthomonas oryzaepv. oryzae. We concluded that S. marcescens did not onlypotent as biocontrol agent to BPH, but also it can be used tocontrol plant pathogenic bacteria.
Potensi Pemanfaatan Perangkat Diagnostik ELISA serta Variannya untuk Deteksi Patogen Tanaman Suryadi, Yadi; Manzila, Ifa; Machmud, muhammad
Jurnal AgroBiogen Vol 5, No 1 (2009): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.517 KB)


Diseases aremajor constrains to agricultural crop productions in Indonesia.In the current free world trade system, the chances ofintroduction of plant quarantine agents are higher, and aredifficult to control, due to importation of seeds and otherplanting materials. Principles of the plant disease controlinclude exclusion and eradication. Early and accurate diseasediagnosis is an early and important step for a successfuldisease control. Enzyme-linked Immunosorbent Assay(ELISA) is a promising technique for an aneffective andefficient disease diagnosis. Some advantages of techniqueover the conventional and molecular diagnostic techniquesare economical use of reagents, high sensitivity, relativelysimple and quick, suitable for large numbers of samples,and adaptable for automation. In the past decade, severalvariants and kits of ELISA had been introduced, such asIndirect ELISA, F(ab’)2 ELISA, Dot Blot ELISA, and ImmunoFluorescence Assay (ELFA). Based on the solid membraneused, the Dot Blot ELISA some variants were developed,such as the NCM-ELISA, Tissue Blotting ELISA, dan Paper ELISA. The ELISA variants had different limit of detection levels. The limit detection of the variants for bacteria is ranging from 102-105 cells/ml, while those for viruses were from 1-10 ng/ml. The times required for the ELISA tests ranging from 5-48 hours. Models and components of ELISA kits for some viral and bacterial plant pathogens had been developed, but more are still needed since generally for each pathogen needs a different kit. The commercially available ELISA kits are limited in numbers, some of themare for pathogens that are not present in Indonesia. Production of ELISA kits for domestic uses will be more effective and efficent, particularly for pathogens that are present in the country. The ELISA kits are applicable not only fo detection and identification of pathogens, but also forecological study of the pathogens in conjuction with epidemiological study of the disease. This paper is a brief review on the ELISA technique and its variants and potential uses for detection of plant pathogens
Peningkatan Ketahanan Tanaman Pisang Kepok Kuning terhadap Penyakit Darah melalui Variasi Somaklonal dan Simbiosis Endofitik Wibowo, Arif; Joko, Tri; Subandiyah, Siti; Mariska, Ika; Supriyati, Yati; Suryadi, Yadi; Roostika, Ika
Jurnal Perlindungan Tanaman Indonesia Vol 16, No 1 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11738


One of the obstacles that was encountered in the banana cultivation is blood disease. Blood diseases is caused by Ralstonia solanacearum that is subsequently revised to become blood disease bacteria (BDB). Until now the control of banana blood disease is very difficult. Control of banana blood disease with chemical injections and soil treatment is not effective. This study was aimed to obtain Kepok Kuning cultivar banana seedlings which was resistant towards blood disease obtained from in vitro selection by using BDB growing filtrate and induced resistance by inoculation of antagonistic endophytic bacteria.The observation of Kepok Kuning banana explants treated with various concentrations of BDB growing filtrate showed that the percentage of living explants decreased to 83.33% when the BDB growing filtrate concentration increased to 15%. Treatment of banana explants with BDB growing filtrate also affected the number of roots, shoots, and leaves. Treatment with a single antagonistic endophytic bacteria suppressed the intensity of banana blood disease to 0% in comparison with the mixture of antagonistic endophytic bacteria if Kepok Kuning banana explants were not treated with BDB growing filtrate. When Kepok Kuning banana explants were treated with BDB growing filtrate, the intensity of banana blood disease suppressed to 0% after the high concentration of BDB growing filtrate and the antagonistic endophytic bacteria mixture were applied. Salah satu kendala yang dihadapi dalam usaha budidaya pisang adalah adanya penyakit darah. Penyakit darah disebabkan oleh bakteri Ralstonia solanacearumyang selanjutnya direvisimenjadi bakteri darah pisang (Blood Disease Bacteria orBDB). Sampai saat ini pengendalian penyakit darah pisang sangatsukar dilakukan. Pengendalian penyakit darah dengan suntikan bahan kimia dan perlakuan tanah tidak efektif untuk diaplikasikan. Penelitian ini bertujuan untukmendapatkan bibit pisang kultivar Kepok Kuning yang tahan penyakit darah yang diperoleh dariseleksi in vitro dengan menggunakan filtrat pertumbuhan BDB dan induksi ketahanan melalui inokulasi jasad renik endofitik yang bersifat antagonis. Hasil pengamatan terhadap eksplan pisang Kepok Kuning yang diperlakukan dengan berbagai konsentrasi filtrat BDB menunjukkan bahwa pada eksplan yang diperlakukan dengan filtrat pertumbuhan BDB, persentase tumbuhnya akan berkurang hingga 83,33% jika konsentrasifiltrat pertumbuhanBDBmencapai 15%. Selain itu perlakuan planlet pisang dengan filtrat pertumbuhan BDB akan mempengaruhi jumlah akar, tunas, dan daun. Perlakuan dengan jasad renik endofitik antagonis secara tunggal mampu menekan intensitas penyakit darah hingga 0% jika dibandingkan dengan perlakuan campuran apabila sebelumnya eksplan pisang Kepok Kuning tidak diperlakukan dengan filtrat pertumbuhan BDB. Apabila sebelumnya planlet pisangKepokKuning diperlakukan dengan filtrat pertumbuhan BDB maka mampu menekan intensitas penyakit darah hingga 0% jika konsentrasi filtrat pertumbuhanBDBsemakin tinggi dan diperlakuan campuran jasad renik endofitik.
PATOGENISITAS ISOLAT BAKTERI Xanthomonas oryzae pv. oryzae DAN PEMANTAUAN PENYAKIT HAWAR DAUN BAKTERI PADA PADI GALUR ISOGENIK [Pathogenicity of Xanthomonas oryzae pv. oryzae Isolates and Bacterial Leaf Blight Disease Monitoring on Rice-Near Isogenic Lines (NILs)] Suryadi, Yadi; Kadir, Triny Suryani
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3426.636 KB) | DOI: 10.14203/beritabiologi.v16i2.2393


Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is an important rice disease due to its high intensity that and  the mereased durability of its resistant variety. This study was aimed  to determine the pathogenicity of Xoo isolates, which obtained from four regions/districts in West Java on three rice near isogenic lines (NILs) containing a single resistance (R) gene; and  to study reaction of rice NILs/ differential genotypes containing mixture of resistant genes to Xoo population under endemic areas. Out of 22 Xoo isolates have been collected and further determined by ELISA assay. Ten Xoo isolates were selected and inoculated to identify their pathogenicity on three NILs i.e., IRBB5 (xa5), IRBB7 (Xa7) and IRBB21 (Xa21). Pathogenicity test showed that most of isolates produced large lesion, and four virulence groups were identified. Phylogenetic analysis suggested that the dominant virulent isolates were widely distributed at several district in West Java. It was shown that 11 NILs exhibited high levels of resistant reaction to the predominant Xoo pathotypes in Cianjur, while 10 lines were susceptible. The single R gene (IRBB7, IRBB21 and Java14) exhibited lower BLB severity. The IRBB50, IRBB51, IRBB52, IRBB53 lines (carrying two R genes), IRBB56, IRBB57 (carrying three R genes); IRBB64 (carrying four R genes), and IRBB66 (carrying five R genes) showed lower severity and thus produced higher resistance to Xoo. This study may implied further work to deploy effective R genes against certain Xoo pathotypes in differ  region.   
EKSPLORASI DAN KARAKTERISASI ENTOMOPATOGEN ASAL BERBAGAI INANG DAN LOKASI [Exploration and Characterization of Entomopathogenic from Various Host and Location] Priyatno, Tri Puji; Samudra, I Made; Manzila, Ifa; Susilowati, Dwi Ningsih; Suryadi, Yadi
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3631.714 KB) | DOI: 10.14203/beritabiologi.v15i1.2859


Microbial groups of entomopathogenic (fungi and bacteria) had been reported causing insect mortality. The aim of the study was to explore and characterized entomopathogenic from various host and locations. Fungal identification at genus and species level was caried out based on conidial morphology, hyphal growth, conidiophore and colony color; whilst for bacterial identification was based on standard Bergey’s manual for determinative bacteria. Sixteen entomopathogenic isolates that consisted of fungal and bacteria have been collected and preserved for further characterization. Of the 16 entomopathogen collected samples, five fungal genera was found i.e. Paecilomyces; Metarhizium, Beauveria, Hirsutella; and Cordyceps. Seven isolates belonging to six fungal isolates, and one bacterial isolate had been identified based upon ITS and 16S rDNA sequences, respectively. We confirmed that 6 fungal isolates belong to species of Paecilomyces reniformis, B. bassiana, M. anisopliae, M. anisopliae var acridum, Hirsutella thomsonii. One isolate of red pigmented bacteria Sm201102 have been identified was belonging to species Seratia marcescence. It was also obtained two fungal isolates from different host (spider and beetle) which confirmed by morphological character belong to Cordyceps sp.
SELEKSI JAMUR PATOGEN SERANGGA Beauveria spp. SERTA UJI PATOGENISITASNYA PADA SERANGGA INANG-WALANG (Leptocorisa acuta) [Selection of Enthomopathogenic Fungi Beauveria spp. and their Pathogenicity Test Against Insect Host-Rice Stink Bug (Leptocorisa acuta)] Wartono, Wartono; Nirmalasari, Cyntia; Suryadi, Yadi
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3848.189 KB) | DOI: 10.14203/beritabiologi.v15i2.2271


Rice-stink bug (Leptocorisa acuta Thumb) that attack rice crop often causing heavy damage of panicle of rice as well as decreasing either quantity or quality of grain after harvest. The objective of this research was to select 14 entomopatogenic fungi of Beauveria spp. isolates, collected from rice stink bug (L. acuta) and their pathogenicity assay to insect host L. acuta. We also aimed to study diversity of Beuveria spp. which was isolated from insect host from Situgede, Bogor. The research was conducted at Laboratory, and Green house of Center for Agricultural Biotechnology and Genetic Resources Research and Development, Bogor, in 2014. Result of the study showed that isolates from Situgede Bogor were sucessfully isolated  as Beauveria spp. based on Koch Postulat assay.  The identified fourteen isolates were morphologically confirmed as Beauveria spp. The pathogenicity test was indicated by symptoms and mortality of rice stink bug after inoculation with these entomopathogenic fungi. The Beuveria isolates Stgd2(14)1, Stgd6(14)1, Stgd7(14)2, Stgd8(14)2, and Stgd0113 were the most virulent isolates. Stgd2(14)1 provided the fastest time to kill L. acuta with LT50 values of 6.9 days. The 13 isolates of Beauveria were well amplified by ITS primers. However, no diversity was found among isolates, presumably due its narrow host range tested. 
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (617.994 KB) | DOI: 10.14203/beritabiologi.v5i1.1090


Raistonia solanacearum,the bacterial wilt pathogen, has a wide host range and genetic variability.Rapid and sensitive molecular techniques need to be developed for eariy detection and strain differentiation of the pathogen.Molecular techniques such as PCR and DNA hybridization have been succesfully used to detect and identify bacterial plant pathogens including R.solanacearum.These techniques were adopted under Indonesian condition, using purified and crude DNA from infected plant samples.An R.solanacearum specific DNA primer (OH/Y2) was used in the PCR test,and a DNA probe 5a67 were used in the non-radioactive hybridization test.The PCR techniqe could be used to detect R.solanacearum from infected plant samples in less than 5 hours.The DNA hybridization technique was applicable to differentiate strains ofR.solanacearum into three groups based on their DNA profiles.