Articles

Respon Fisiologi Benih Ikan Kerapu Macan Epinephelus fuscoguttatus Terhadap Penggunaan Minyak Sereh dalam Transportasi Tertutup dengan Kepadatan Tinggi

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 15, No 2 (2010): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Ikan Kerapu Macan merupakan salah satu komoditas ikan air laut yang cukup digemari oleh masyarakat dan memiliki nilai ekonomis yang tinggi. Masalah yang sering dihadapi oleh petani adalah sulitnya mendapatkan benih ikan kerapu yang cukup baik karena jarak antara tempat pembesaran dan tempat pembenihan relatif jauh. Oleh karena itu dibutuhkan teknologi yang tepat yang dapat mengangkut ikan dalam waktu yang lama, tingkat kelangsungan hidup yang tinggi serta kondisi fisiologi ikan pasca pengangkutan yang tetap baik. Penelitian ini bertujuan untuk menganalisis dampak penggunaan minyak sereh terhadap respon fisiologi berupa gambaran darah, histologi jaringan dan pertumbuhan serta kelangsungan hidup  benih ikan kerapu macan dengan ukuran panjang rata-rata 7 cm dan berat rata-rata 4,02 gram yang diangkut di dalam sistem tranportasi tertutup dengan kepadatan tinggi selama 56 jam. Rancangan acak lengkap digunakan dengan 4 perlakuan, yaitu tanpa minyak sereh (Kontrol) minyak sereh 10, 20 dan 30 mg/L dengan 2 ulangan. Hasil penelitian menunjukkan bahwa penambahan minyak sereh 10 mg/L lebih baik dibandingkan perlakuan yang lain, baik dilihat dari kualitas air dengan nilai Total Ammonia Nitrogen (TAN) terendah  6,459±1,290 mg/L, CO2 32,561±6,498 mg/L, maupun dari kondisi fisiologi berupa kadar glukosa 50,375±28,390 mg/dl, nilai gambaran darah berupa sel darah merah 1,28x106 sel/mm3, sel darah putih 2,60x104 sel/mm3, N:L (Netrofil:Limfosit) rasio 0,41% yang mendekati nilai kondisi ikan normal,  kondisi histologi berupa  tingkat kerusakan insang yang paling rendah  dan nilai SR tertinggi 97,5% serta laju pertumbuhan 1,33%. Kata kunci: kerapu macan, minyak sereh, respon fisiologi, transportasi tertutup, kepadatan tinggi Tiger Grouper is one of marine fish commodities well-loved by the community and have high economic value. The problem often faced by grouper farmers is the difficulty to obtain the good seed because the distance between the place where the hatchery rearing and relatively remote. Therefore, it needs a proper technology to transport the fish for a long time, the survival rate is high and the condition of post-transport physiology of fish that remain good. This study was aimed to evaluate the impact of the use of citronella oil on the physiological responses of the juvenile tiger grouper with emphasized on the evaluation of blood characteristics, histophatological change, growth and survival rate of the fish.  The fish with an average length of 7 cm and an average of 4,02 grams in weight which are transported in high density of sealed transportation system for 56 hours. Completely randomized design (CRD) was applied with 4 treatments (Without citronella oil (K/Control), 10, 20, 30 mg/L of citronella oil respectively and 2 replications. The results showed that application of 10 mg/L of citronella oil was better than the other concentration, in terms of water quality with low Total Ammonia Nitrogen (TAN) value 6.459 ± 1.290 mg/L, CO2 32.561 ± 6.498 mg/L, and the physiological conditions  such as glucose level was  50,375 ± 2.390 mg/dL , red blood  cell  value was 1,28x106 mm3, leucocyte  value was 2,60x104 mm3, N: L ratio was 0.41%, the lowest gill damage and the highest survival rate and growth rate ; those conditions were  close to the normal fish. Key words:    juvenile tiger grouper, citronela oil, physiological responses, sealed transporatation system, high density

Effect of LH-RHa Injection trough W/O/W LG (C-14) Emulsion on Gonad Development of Catfish (Pangasious hypophthalmus)

Jurnal Akuakultur Indonesia Vol 3, No 3 (2004): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

The objective of this research was to study the effect of LHRHa gonad maturity in broodstock of P. hypophthalmus through W/O/W LG (C-14) emulsion injection. The treatments consisted of control (A), 50 µg/kg fish wight (B), and 100 µg/kg fish weight (C), with five replications of each. Fish weight at the beginning of experiment was 2.0 kg/individual. The result showed that LHRHa in W/O/W emulsion had positive effect on egg maturation. This was shown by the value of average of eggs diameter. The maximum size of egg diameter for fish 701.52 ± 17.56 µm. The size of eggs was more homogenous in group B than those of group C and A. it was concluded that injection of LHRHa in W/O/W LG (C-14) emulsion if effective in promoting gonad maturation and oocyte development in the catfish

Blood Glukose Response of Giant Gouramy (Osphronemus gaouramy, Lac.) to the Stress of Environmental Temperature Changes

Jurnal Akuakultur Indonesia Vol 2, No 2 (2003): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

This experiment was conducted to investigate blood glucose performance of giant gouramy (Osphronemus gouramy, Lac.) to environmental changes. Fish with body weight of about 52,15 g was used in the experiment. A hundred and twenty fish were subjected to stress by moving them to another aquarium containing cooler water for 5 minute before put them back to the origin aquarium. The stress treatments were Δ 0°C (A), Δ-3°C (B), Δ-6°C(C), and Δ-9°C(D). Blood glucose was measured at 0, 1, 2, 3, 4 and 5 hours post stress, each for 5 fish. During stress treatment, the survival offish were recorded. To study the role of insulin activation on reducing the stress effects, thirty fish were injected with insulin 2 IU/100 g body weight before subjected them to stressar. Blood glucose level of fish subjected to temperature stress of Δ-9°C was the greatest. The blood glucose response to temperature changes was linear, Y = 4,4543 X + 35,553 with R2 = 0,09976. The survival rate of fish was 100% for all treatments. Injected of insulin 2 IU/100 g body weight was able to reduce hyperglycemia that caused by stress. Key words: Blood glucose, giant gouramy, Osphronemus gouramy, stress   ABSTRAK Penelitian ini dilakukan dengan tujuan untuk mengetahui performa glukosa darah ikan gurami (Osphronemus gouramy, Lac.) dalam merespon perubahan suhu lingkungan. Ikan berbobot rata-rata 52,15 g sebanyak 120 ekor diberi stres dengan cara diangkat dan dipindahkan ke suatu wadah yang bersuhu lebih dingin selama 5 menit dan dikembalikan lagi ke wadah mula-mula. Perlakuan stres perubahan suhu dingin tersebut adalah A (Δ 0°C), B (Δ- 3°C), C (Δ-6°C) dan D (Δ-9°C). Glukosa darah diukur dari 5 ekor ikan pada jam ke 0, 1, 2, 3, 4 dan 5 jam pascastres. Kelangsungan hidup dihitung pada saat perlakuan stres. Untuk melihat peran aktivasi insulin dalam menekan efek stres, ikan sebanyak 30 ekor diinjeksi insulin 2 iu/100 g bobot badan sebelum diberi stres. Kadar glukosa darah ikan gurame yang diberi stres perubahan suhu dingin sebesar Δ-9°C mengalami peningkatan paling besar. Respon glukosa darah terhadap stres perubahan suhu tersebut berpola linier Y= 4,4543 X + 35,553, dengan R2 = 0,9976. Perlakuan tersebut menghasilkan kelangsungan hidup yang sama yaitu 100%. Injeksi insulin 2 IU/100 g bobot badan mampu menekan hiperglisemia akibat stres. Kata kunci: Glukosa darah, ikan gurami, Osphronemus gouramy, stres

Utilization of MS 222 in Transport of Catfish (Pangasius sutchi) Seed

Jurnal Akuakultur Indonesia Vol 1, No 3 (2002): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

Problems faced in supplying seed out of Java especially Bogor to Sumatera, for instance, are it only contains few seed (100 seeds/l) and it has a high mortality rate because of stress during transport.  This experiment was conducted to know the effect of MS 222 in keeping fish metabolism activity down while taking note of its survival rate and change of water quality. The experimental design used here was factorial design 3x5 with 3 repetitions.  Dosages of MS–222 were 0, 25 and 50 ppm and the fish densities were 100, 300, 400, 500 and 600 per liter.  As container was plastic bag (volume 10 l).  After putting the fish into the plastic bag, it was filled up with oxygen, which the volume was 3 times than water volume, than the plastic bag was closed by tying it.  After 18 hours of treatment the survival rate and water quality were checked.  Based on evaluation of survival rate, it was concluded that the combination between 25 ppm of MS 222 and fish density, which was 500 per liter, gave an optimum result. Keywords: Catfish, Pangasius sutchi, live transport, stress, MS–222, survival rate   ABSTRAK Kendala yang dihadapi dalam pemasokan benih ikan patin dari pulau Jawa terutama Bogor, keluar pulau Jawa terutama Sumatera adalah jumlah ikan yang terangkut masih sedikit (± 100 ekor/l) dan tingkat kematian yang cukup tinggi, karena stres selama perjalanan. Penelitian ini dilakukan untuk mengetahui pengaruh penggunaan MS–222 dalam menekan aktivitas metabolisme ikan dengan memperhatikan tingkat kelangsungan ikan dan perubahan kualitas air. Penelitian ini menggunakan rancangan faktorial 3x5 dengan 3 ulangan. Dosis MS–222 yang digunakan: 0,25 dan 50 ppm dan kepadatan ikan: 100, 300, 400, 500 dan 600 ekor/l. Wadah pengangkutan berupa kantong plastik (volume 10 l). Ikan yang diangkut dimasukkan ke dalam kantong plastik, kemudian dipompakan oksigen sebanyak 3 kali volume air, selanjutnya dilakukan pengikatan. Setelah 18 jam perlakuan dilakukan perhitungan terhadap tingkat kelangsungan hidup ikan dan perubahan kualitas air media. Hasil penelitian ini menunjukkan kombinasi penggunaan MS–222 25 ppm dan kepadatan ikan 500 ekor/l memberikan tingkat kelangsungan hidup yang optimal. Kata kunci: Ikan patin, Pangasius sutchi, pengangkutan hidup, stres, MS–222, tingkat kelangsungan hidup.

Protein digestibility and ammonia excretion in catfish Clarias gariepinus culture

Jurnal Akuakultur Indonesia Vol 12, No 1 (2013): Jurnal Akuakultur Indonesia
Publisher : ISSA

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Abstract

ABSTRACT A series of experiments was performed to analyze protein digestibility, ammonia excretion, and also heterothropic bacteria and phytoplankton dynamics in the catfish Clarias gariepinus culture. In the digestibility experiment, catfish with an individual initial size of 43.67±0.83 g were stocked into 120 L conical fiberglass tanks at a density of 20 fish per tank. Fish were fed on with commercial diet supplemented with Cr2O3 indicator at a concentration of 1%. In the ammonia excretion experiment, catfish with an individual size of 111.6±9.5 and 40.6±3.4 g, respectively,  were placed into a 10 L chamber filled with 8 L of water. Total ammonia nitrogen (TAN) in the chambers were monitored every hour for six consecutive hours. In the bacteria and phytoplankton dynamics experiment, catfish were stocked in the 25 m2 concrete tanks which was divided into two compartments (catfish 10 m2, and heterotrof compartments 15 m2). Catfish with individual size of 42,5±0 g were stocked into the tanks at a density of 100 fish per tank. Water was recirculated from catfish compartments to heterotrophic compartments. Fish were fed with floating feed. Molasses as carbon source for heterotrophic bacteria was applied daily. The experiment was conducted for six weeks. The results showed that the protein digestibility was 61.97±7.24%. Larger fish (size of 111.6 g) excreted ammonia at a rate of 0.008±0.003 mg TAN/g fish-weight/hour, which was lower than that of the smaller catfish (size of 40.6 g), i.e. 0.012±0.004 mg TAN/g fish-weight/hour. Keywords: protein digestibility, ammonia excretion, catfish  ABSTRAK Serangkaian penelitian telah dilakukan untuk menganalisis ketercernaan pakan dan protein, ekskresi amonia, serta dinamika bakteri dan fitoplankton pada budidaya ikan lele (Clarias gariepinus). Pada penelitian ketercernaan pakan, ikan lele berukuran 43,67±0,83 g/ekor dipelihara dalam bak fiberglas berbentuk corong berukuran 120 L dengan kepadatan 20 ekor/bak. Ikan diberi pakan berupa pelet yang diberi indikator Cr2O3 sebanyak 1%. Pada penelitian ekskresi amonia, ikan lele berukuran 111,6±9,5 dan 40,6±3,4 g/ekor yang telah diberi makan sampai kenyang dimasukkan ke dalam stoples berisi 8 L air. Kadar amonia total (total ammonia nitrogen, TAN) di dalam stoples diukur setiap jam selama enam jam. Pada penelitian dinamika bakteri dan fitoplankton, ikan lele dipelihara pada bak beton berukuran 25 m2 yang disekat menjadi dua bagian yaitu bagian ikan lele (10 m2) dan bagian heterotrof (15 m2). Ikan lele dengan bobot awal 42,5 g/ekor ditebar ke dalam bak dengan kepadatan 100 ekor/bak. Air mengalir secara resirkulasi dari bagian ikan lele ke bagian heterotrofik dengan bantuan pompa. Pakan yang diberikan berupa pelet apung komersial. Molase ditambahkan setiap hari sebagai sumber karbon untuk pertumbuhan bakteri heterotrofik. Penelitian dilaksanakan selama enam minggu. Hasil pengamatan menunjukkan bahwa ketercernaan protein dari pakan yang diuji adalah 61,97±7,24%. Ikan lele berukuran besar (111,6 g/ekor) menghasilkan amonia sebanyak 0,008±0,003 mg TAN/g ikan/jam, lebih rendah dibandingkan dengan ikan yang berukuran lebih kecil (40,6 g/ekor), yaitu 0,012±0,004 mg TAN/g ikan/jam.  Kata kunci: ketercernaan protein, ekskresi amonia, ikan lele

Acute and sublethal toxicity of copper in Siam-catfish juvenile Pangasianodon hypophthalmus

Jurnal Akuakultur Indonesia Vol 13, No 1 (2014): Jurnal Akuakultur Indonesia
Publisher : ISSA

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Abstract

ABSTRACT This study was aimed to determine the level of acute toxicity (LC50) and to analyze the effect of Cu on the survival, growth, haematological, and bioaccumulation of Cu in Pangasianodon hypophthalmus juvenile at sublethal conditions. The experiment trial used completely randomized design with five concentration treatments and two replications to determine LC50. The Cu concentration used were 0, 0.2, 0.7, 1.2, and 1.7 ppm. Sublethal treatment used four Cu concentrations with three replicates. The concentrations were 0, 0.167, 0.334 and 0.667 ppm. Experimental fish used had an average total length 11.0±1.7 cm and weight 13.00±1.72 g. Results showed that Cu was highly toxic to P. hypophthalmus with a LC50-96 hours value of 0.667 ppm (0.539–0.805 ppm). At sublethal concentrations, Cu significantly decreased survival and growth, and increased Cu accumulation started from concentration of 0.167 ppm. Bioaccumulation of Cu started from the highest were in liver, gills, skin and flesh, respectively. Decreased of erythrocytes, haemoglobin and hematocrit indicated anemia, while increased of leukocytes indicated infection and physical stress on the body tissues. Keywords: copper toxicity, haematological, bioaccumulation, Siam-catfish juvenile  ABSTRAK Penelitian ini bertujuan untuk menentukan tingkat toksisitas akut (LC50) dan menganalisis pengaruh Cu terhadap sintasan, pertumbuhan, kondisi hematologi, dan akumulasi Cu pada juvenil ikan patin siam (Pangasianodon hypophthalmus) pada kondisi subletal. Rancangan penelitian yang digunakan adalah lima perlakuan konsentrasi dengan dua ulangan untuk menentukan LC50. Konsentrasi Cu yang digunakan adalah 0; 0,2; 0,7; 1,2; dan 1,7 ppm. Pemeliharaan subletal menggunakan empat variasi konsentrasi dengan tiga ulangan. Konsentrasi Cu yang digunakan adalah 0; 0,167; 0,334; dan 0,667 ppm. Ikan uji yang digunakan memiliki panjang total rata-rata 11,0±1,7 cm dan berat 13,00±1,72 g. Hasil menunjukkan bahwa Cu sangat toksik terhadap juvenil P. hypophthalmus dengan nilai LC50-96 jam sebesar 0,667 ppm (0,539–0,805 ppm). Pada konsentrasi subletal, Cu berpengaruh nyata terhadap penurunan sintasan dan pertumbuhan serta menyebabkan peningkatan akumulasi Cu mulai pada konsentrasi 0,167 ppm. Bioakumulasi Cu secara berurutan mulai dari yang tertinggi yaitu pada hati, insang, kulit dan daging. Penurunan eritrosit, hemoglobin dan hematokrit menunjukkan terjadinya anemia, sementara peningkatan jumlah leukosit menunjukkan infeksi dan stres fisik pada jaringan tubuh. Kata kunci: toksisitas tembaga, hematologi, bioakumulasi, bioakumulasi, sintasan, juvenil patin siam

Evaluation of integrated sludge worm and catfish farming with biofloc system

Jurnal Akuakultur Indonesia Vol 13, No 1 (2014): Jurnal Akuakultur Indonesia
Publisher : ISSA

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Abstract

ABSTRACTCurrently, supply of sludge worm (Tubifex sp.) merely came from wild, so that it could not meet demand for fish hatchery. Additionally, harvest from the wild did not have a quality assurance, due to the possibility of sludge worm becoming an agent of disease. This study was conducted to evaluate the production of sludge worm culture by utilizing catfish culture waste (Clarias sp. ) in bioflok system. In this system, water from catfish culture media flowed into sludge worm culture media using recirculation systems. This study used a completely randomized design with four treatments and two replications, in which treatment A (addition of catfish culture waste from intensive system), treatment B (addition of catfish culture waste from biofloc system), treatment C (the addition of fermented chicken manure at the beginning of experiment as negative control), and treatment D (addition of fermented chicken manure at the beginning of experiment and then every five days as positive control). The results showed that the highest production was obtained by treatment B at biomass growth of 0.97 kg/m2 and sludge worm density of 388.000 individu/m2. Keywords: sludge worm, biofloc, chicken manure, catfish, catfish culture waste  ABSTRAK Saat ini pasokan cacing sutra (Tubifex sp.) hanya berasal dari alam, sehingga belum mencukupi permintaan untuk kegiatan pembenihan ikan. Selain itu, hasil tangkapan dari alam tidak memiliki jaminan kualitas, karena cacing sutra dapat menjadi agen penyakit. Penelitian ini dilakukan untuk mengevaluasi hasil produksi budidaya cacing sutra dengan memanfaatkan limbah budidaya ikan lele (Clarias sp.) sistem bioflok. Pada sistem ini, air dari media budidaya ikan lele dialirkan ke media pemeliharaan cacing sutra menggunakan sistem resirkulasi. Penelitian ini menggunakan rancangan acak lengkap dengan empat perlakuan dan dua ulangan, yaitu perlakuan A (pemberian limbah ikan lele sistem intensif), perlakuan B (pemberian limbah ikan lele sistem bioflok), perlakuan C (penambahan fermentasi kotoran ayam pada awal penelitian sebagai kontrol negatif), serta perlakuan D (penambahan fermentasi kotoran ayam pada awal penelitian yang dilanjutkan setiap lima hari sekali sebagai kontrol positif). Hasil penelitian menunjukkan bahwa produksi cacing sutra tertinggi diperoleh pada perlakuan B dengan pertumbuhan bobot sebesar 0,97 kg/m2 dan kepadatan cacing sutra sebesar 388.000 individu/m2. Kata kunci : cacing sutra, bioflok, kotoran ayam, ikan lele, limbah budidaya ikan lele

The effectiveness of Lemna perpusilla as phytoremediation agent in giant gourami culture media on 3 ppt

Jurnal Akuakultur Indonesia Vol 14, No 2 (2015): Jurnal Akuakultur Indonesia
Publisher : ISSA

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ABSTRACT The wasted from feed and feces containt nitrogen and phosphorus can decreased fertility and feability water quality. Lemna perpusilla (duckweed) is prospective to use as an agent of phytoremediation of organic waste and can used as animal feed because it has high protein content. Meanwhile water salinity could be accelerate the growth of giant gourami. The aim of this research was to analyze the ability of L. perpusilla in absorbing nutrients nitrogen and phosphorus in water salinity of 3 ppt. The research was conducted four treatments and three replications. The treatments were A (L. perpusilla and 3 ppt salinity), B (L. perpusilla, 3 ppt salinity and filter), C (L. perpusilla, 3 ppt salinity and aeration), and D (L. perpusilla, 3 ppt salinity, filter and aeration). Experiment were carried in aquaria 50×33×50 cm3 in size with density of gourami fish 150/49.5 L for one month. The results showed that the ability of L. perpusilla to absorb N and P decreased from the beginning of the study due to lack of nutrient source of N and P in the aquaculture media, but increased because the impact of the feeding and  metabolism of the gourami. There was no different treatment effect for decreased N and P (P> 0.05). The highest nitrite level was found in D treatment, it means that L. perpusilla not be able to absorb  N and P in the media 3 ppt salinity. However, the addition of 3 ppt salinity gives the best results for the survival rate and feed efficiency ratio. Keywords: phytoremediation, Lemna perpusilla, giant gourami fish, nitrogen and phosphorus  ABSTRAK Limbah pakan dan feses yang mengandung nitrogen dan fosfor dapat menyebabkan penurunan kesuburan dan kelayakan kualitas air. Lemna perpusilla (duckweed) baik digunakan sebagai agen fitoremediasi organik untuk limbah dan dapat digunakan sebagai pakan hewan karena mengandung protein yang tinggi, sementara media bersalinitas mampu mempercepat pertumbuhan ikan gurami. Tujuan dari penelitian ini adalah untuk menganalisis kemampuan L. perpusilla dalam mengabsorbsi nutrisi nitrogen dan fosfor pada air bersalinitas 3 ppt. Penelitian ini terdiri atas lima perlakuan dan tiga ulangan. Perlakuan yang diberikan adalah A (L. perpusilla dan salinitas 3 ppt), B (L. perpusilla, salinitas 3 ppt dan filter), C (L. perpusilla, salinitas 3 ppt dan aerasi), dan D (L. perpusilla, salinitas 3 ppt, aerasi dan filter). Akuarium yang digunakan berukuran 50×33×50 cm3 dengan kepadatan ikan gurami 150 ekor/49,5 L dan waktu pemeliharaan selama satu bulan. Hasil penelitian ini menunjukkan bahwa kemampuan L. perpusilla menyerap limbah N dan P berkurang dari awal penelitian karena kurangnya sumber nutrisi N dan P pada media pemeliharaan, namun beranjak meningkat yang berdampak dari adanya pemberian pakan dan sisa metabolisme dari ikan gurame. Tidak ada perlakuan yang berpengaruh terhadap pengurangan N dan P (P>0,05). Nilai nitrit tertinggi terdapat pada perlakuan D, hal ini berarti bahwa L. perpusilla tidak mampu untuk menyerap limbah N dan P pada media bersalinitas 3 ppt. Namun penambahan salinitas 3 ppt memberikan hasil yang terbaik bagi derajat kelangsungan hidup ikan gurami dan efisiensi pakan. Kata kunci: fitoremediasi, Lemna perpusilla, ikan gurami, nitrogen dan fosfor 

The use of zeolite, active carbon, and clove oil in closed transportation of giant freshwater prawn juvenile

Jurnal Akuakultur Indonesia Vol 14, No 1 (2015): Jurnal Akuakultur Indonesia
Publisher : ISSA

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Abstract

ABSTRACT The objective of this study was to determine the effect of zeolite, active carbon, and clove oil on water quality (dissolved oxygen/DO, total ammonia nitrogen/TAN, temperature) and biological quality (glucose concentration, total protein, survival/SR) of giant freshwater prawn juvenile Macrobrachium rosenbergii in closed transportation system. The study was conducted in laboratory scale with a completely randomized design. The biota used was juvenile giant prawn with an average weight 0.407 ± 0.005 g/ind. The type and dose of additive used were A (20 g/L zeolite + 10 g/L active carbon + 14 µL/L clove oil), B (20 g/L zeolite + 10 g/L active carbon + 9.33 µL/L clove oil), C (20 g/L zeolite + 10 g/L active carbon + 4.67 µL/L clove oil), D (20 g/L zeolite + 10 g/L active carbon + 1.87 µL/L clove oil), K+ (20 g/L zeolite + 10 g/L active carbon), and K- (without material addition). The glucose concentration of treatment B and C significantly different with treatment A, D, K+, K-. Total protein of treatment A, B, C and K+ significantly different with treatment K-. DO, TAN, and temperature of the transportation media were still in the suitable concentration for living of giant prawn. The highest survival of the prawn was observed in group C. The result showed the combination of 20 g/L zeolite + 10 g/L active carbon + 4.67 µL/L clove oil in the water is suitable for closed transportation system for juvenile giant freshwater prawn. Keywords: glucose concentration, total protein, DO, TAN, temperature  ABSTRAK Penelitian ini bertujuan untuk menentukan pengaruh pemberian zeolit, karbon aktif, dan minyak cengkeh terhadap kualitas air (dissolved oxygen/DO, total ammonia nitrogen/TAN, suhu) dan kualitas biologi (konsentrasi glukosa, total protein, tingkat kelangsungan hidup/TKH) benih udang galah Macrobrachium rosenbergii pada sistem transportasi tertutup. Penelitian dilakukan pada skala laboratorium dengan rancangan acak lengkap. Biota yang digunakan yaitu benih udang galah dengan bobot rata-rata 0,407±0,005 g/ekor. Dosis bahan tambahan yang digunakan adalah: A (20 g/L zeolit + 10 g/L karbon aktif + 14 µL/L minyak cengkeh), B (20 g/L zeolit + 10 g/L karbon aktif + 9,33 µL/L minyak cengkeh), C (20 g/L zeolit + 10 g/L karbon aktif + 4,67 µL/L minyak cengkeh), D (20 g/L zeolit + 10 g/L karbon aktif + 1,87 µL/L minyak cengkeh), K+ (20 g/L zeolit + 10 g/L karbon aktif), dan K- (tanpa bahan tambahan). Konsentrasi glukosa perlakuan B dan C berbeda nyata dengan perlakuan A, D, K+, K-. Total protein perlakuan A, B, C, D, dan K+ berbeda nyata dengan perlakuan K-. DO, TAN dan suhu media transportasi masih sesuai dengan kehidupan udang galah. Tingkat kelangsungan hidup transportasi tertinggi yaitu pada perlakuan C. Hasil menunjukkan bahwa kombinasi 20 g/L zeolit + 10 g/L karbon aktif + 4,67 µL/L minyak cengkeh adalah perlakuan yang sesuai untuk transportasi tertutup benih udang galah. Kata kunci: konsentrasi glukosa, total protein, DO, TAN, suhu

Organic carbon source and C/N ratio affect inorganic nitrogen profile in the biofloc-based culture media of Pacific white shrimp (Litopenaeus vannamei)

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 1 (2016): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Organic carbon source and C/N ratio play an important role in aquaculture system with biofloc technology application. Nitrogen control by adding carbohydrates to the water to stimulate heterotrophic bacterial growth by converting nitrogen into bacterial biomass. The study investigated the effect molasses, tapioca, tapioca by product and rice bran as carbon sources in a biofloc media at three different C/N ratios i.e. 10, 15, and 20 on total ammonia reduction in biofloc media. Five liters of biofloc media in a conical tank was prepared for each replicate, which consisted of 500 mL of biofloc suspension collected from a shrimp culture unit with biofloc technology application and 4.5 L seawater. Pacific white shrimp culture was performed in 40L glass aquaria at a shrimp density of 30/aquarium. There was a significant interaction between carbon source and the C/N ratio applied (P<0.05). The use of molasses resulted in the highest reduction rate irrespective to the C/N ratio. Keywords: molasses, tapioca, tapioca by product, rice bran, biofloc, total ammonia nitrogen

Co-Authors . Subandiyono . SUKENDA . Sulistiono . WIDANARNI ., Berlianti Alexander Burhani Marda, Alexander Burhani Ardi, Idil Bambang Gunadi Budiyanti Budiyanti CECEP KUSMANA Daniel Djokosetianto Darmansah, Aris Dedi Jusadi Dedi Pardiansyah Diana Sriwisuda Putri Dinamella Wahjuningrum Djai, Suhaiba Dody Sihono Eka Rosyida Enang Harris Enang Harris Surawidjaja, Enang Harris Ernik Yuliana Fendjalang, Sophia N.M. Ferdinand Hukama Taqwa Ghozali, M. Faisol Riza Haris, Enang HARTON ARFAH Hasan, O.D. Subakti Humairani, . I Wayan Nurjaya Iman Rusmana Ing Mokoginta Irzal Effendi Jr., Muhammad Zairin JULIE EKASARI Koesoemadinata, Santosa Kristanto, Anang Hari Kukuh Adiyana, Kukuh Kukuh Nirmala Lukmini, Aisyah M. Agus Suprayudi M. Toelihere M. Yusuf Arifin, M. Yusuf M. Zairin Junior Mariam, Susanti Mas Tri Djoko Sunarno, Mas Tri Djoko Mia Setiawati Muhamad Yamin MUHAMMAD AGUS SUPRAYUDI Muhammad Hanif Azhar, Muhammad Hanif MUHAMMAD ZAIRIN JUNIOR Munti Yuhana N. Potalangi Novita, Ris Dewi Nurul Taufiqu Rochman ODANG CARMAN Palinggi, Neltje Nobertine Pamungkas, Amin Petrus Rani Pong-Masak Prama, Ega Aditya Pratiwi, Rifqah Puspaningsih, Dewi Rahma Vida Anandasari, Rahma Vida Rahmawati, Riani Ridwan Affandi S. Hastuti Saputra, Adang Saputra, Henry Kasmanhadi Sukenda Sukenda Sukenda, nFN Surawidjaja, Enang H. Suseno, Sugeng H. Sutrisno sutrisno Syahputra, Ruspindo Tatag Budiardi Thesiana, Lolita Thomas Nugroho Usman Usman Utomo, Bambang Priyo Wahyu, nFN Warlina, Lina Widanarni . Widiyati, Ani Wiramiharja, Yoyo Yosmaniar, nFN Yosmaniar, Yosmaniar