Articles

Found 10 Documents
Search

PENGGUNAAN BAP DAN TDZ UNTUK PERBANYAKAN TANAMAN GAHARU (Aquilaria malaccensis Lamk.) (The Use of BAP and TDZ for Propagation of Agarwood (Aquilaria malaccensis Lamk.)) Azwin, .; Siregar, Iskandar Z.; Supriyanto, .
MEDIA KONSERVASI Vol 11, No 3 (2006): Media Konservasi
Publisher : MEDIA KONSERVASI

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Agarwood (A. malaccensis Lamk.) is one of the important tropical forest trees, which produces a high economically valuable fragrant resinous wood. The increase of agarwood demand from year to year leads to uncontrolled illegal harvest of this plant from its natural habitat. To encounter this problem, there is an urgent need to develop agarwood plantation. Tissue culture is an alternative method to provide genetically good seedlings for plantation in the future due to its short period and mass quantity of planlet production. In addition, through this method, its might also provide homogenous plant, and free pest and diseases. The objectives of the study were (1) to find out the optimal concentration of BAP or TDZ for inducing shoot multiplication of agarwood in in vitro conditions. MS (Murashige And Skoog, 1962), was used as basal media. The experimental design of the research was completely randomized design (RAL) with treatment of BAP concentration (control; 0,50 ppm; 0,75 ppm; 1,0 ppm) or TDZ concentration (control; 0,25 ppm; 0,50 ppm; 0,75 ppm), in 3 units, of replicate every units consist of 4 bottles, every bottle containing one explants coming from axillaries and adventitious shoot explants. Results indicated that two types of agarwood explants grown in vitro in MS basal media containing BAP 0,50 ppm or TDZ 0,25 ppm produced the highest number of shoots and leaves of agarwood plantlets, as well as its plantlet shoot length.Keywords : BAP , TDZ , Agarwood, In Vitro.
Teknik Pangkas Akar untuk Meningkatkan Produksi Bibit Melinjo Bermikoriza Wulandari, Arum Sekar; Supriyanto, .
Jurnal Ilmu Pertanian Indonesia Vol 18, No 3 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mycorrhizal symbiosis is the result of mutualistic interactions between plants and fungi. Melinjo (Gnetum gnemon) naturally associate with ectomycorrhizal Scleroderma sinnamariense and Scleroderma sp. fungi. Artificial inoculation can be applied to young seedlings (1-2 months old), before the roots contain woody material. The purpose of this study was to apply the root pruning techniques to improve gnetum mycorrhizal seedlings production. The study was conducted in a greenhouse using 7 months old seedlings. The treatments consisted of 3 root pruning levels (0, 30, and 50%) and ectomycorrhizal fungi inoculum (control, mycorrhizal seedlings, and soil inoculum). Observations were carried out for 4 months to the seedling growth and root colonization by ectomycorrhizal fungi. Height, diameter, and biomass of melinjo seedlings in all treatments were not significantly different. Thus, root pruning activities do not affect the growth of seedlings. Artificial inoculation produce mycorrhizal seedlings, whereas controls were uninfected. Root pruning increased number of root branching. Root pruning at level 50% increased mycorrhizal seedlings production, but the percentage of root colonization was not significantly difefernt at all pruning levels. Keywords: Gnetum gnemon, mycorrhiza, root pruning, Scleroderma
Productivity and Nutrient Quality of Some Sorghum Mutant Lines at Different Cutting Ages Puteri, Rizki Eka; Karti, Panca Dewi Manu Hara; Abdullah, Luki; Supriyanto, .
Media Peternakan - Journal of Animal Science and Technology Vol 38, No 2 (2015): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/medpet.v38i2.8533

Abstract

The objective of the study was to explore the appropriate cutting age to produce optimal biomass and good nutrient quality from sorghum mutant lines BMR i.e., PATIR 3.5 M7, PATIR 3.6 M7, and PATIR 3.7 M7, also SAMURAI I (M17). A completely randomized in Split Plot design with 2 factors and 3 replicates was used. The first factor was the type of sorghum (SAMURAI I M17, PATIR 3.5, PATIR 3.6, PATIR 3.7) as the main plot and the second factor was the cutting age (85, 95, 105) as a subplot. Parameters observed were the production of stems, leaves, grains, total biomass production, ash, crude fat, crude fiber, crude protein, NFE, TDN, percentage of DMD, OMD and N-NH3. Data were analyzed by using ANOVA followed by DMRT (Duncan Multiple Range Test). The results showed that there were highly significant interactions (P<0.01) between cutting age and type of sorghum in production of stems, leaves, grains, total biomass production, value of TDN, DMD, OMD, and N-NH3. Increasing cutting age significantly increased the percentage of ash content, crude protein and crude fat. The sorghum type significantly affected crude fat content nonBMR sorghum variety of SAMURAI I (M17) and achieved optimal biomass production and nutrient content at cutting age of 85 d similar to BMR sorghum mutant lines PATIR 3.6 and PATIR 3.5, whereas BMR sorghum mutant lines of PATIR 3.7 achieved optimum production at the age of 95 d of cutting. All types of sorghum varieties was not recommended to be harvested at 105 d. Biomass production increased with the increasing of cutting age, but the nutrient content decreased. Key words: cutting age, mutant, sorghum
Productivity and Nutrient Quality of Some Sorghum Mutant Lines at Different Cutting Ages Puteri, Rizki Eka; Karti, Panca Dewi Manu Hara; Abdullah, Luki; Supriyanto, .
Media Peternakan Vol 38, No 2 (2015): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/medpet.2015.38.2.132

Abstract

The objective of the study was to explore the appropriate cutting age to produce optimal biomass and good nutrient quality from sorghum mutant lines BMR i.e., PATIR 3.5 M7, PATIR 3.6 M7, and PATIR 3.7 M7, also SAMURAI I (M17). A completely randomized in Split Plot design with 2 factors and 3 replicates was used. The first factor was the type of sorghum (SAMURAI I M17, PATIR 3.5, PATIR 3.6, PATIR 3.7) as the main plot and the second factor was the cutting age (85, 95, 105) as a subplot. Parameters observed were the production of stems, leaves, grains, total biomass production, ash, crude fat, crude fiber, crude protein, NFE, TDN, percentage of DMD, OMD and N-NH3. Data were analyzed by using ANOVA followed by DMRT (Duncan Multiple Range Test). The results showed that there were highly significant interactions (P<0.01) between cutting age and type of sorghum in production of stems, leaves, grains, total biomass production, value of TDN, DMD, OMD, and N-NH3. Increasing cutting age significantly increased the percentage of ash content, crude protein and crude fat. The sorghum type significantly affected crude fat content nonBMR sorghum variety of SAMURAI I (M17) and achieved optimal biomass production and nutrient content at cutting age of 85 d similar to BMR sorghum mutant lines PATIR 3.6 and PATIR 3.5, whereas BMR sorghum mutant lines of PATIR 3.7 achieved optimum production at the age of 95 d of cutting. All types of sorghum varieties was not recommended to be harvested at 105 d. Biomass production increased with the increasing of cutting age, but the nutrient content decreased.Key words: cutting age, mutant, sorghum
Profil Kekuatan Usaha Dalam Memanfaatkan Peluang Usaha Industri Kecil di Pasar Supriyanto, .
e-JKPP Vol 1, No 2 (2015): Agustus
Publisher : Universitas Bandar Lampung (UBL)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

In understanding business positions to be seen from internal and external environment of small industries, a research on the Profile of Business Strength in Using Business Opportunities in Small Industries in Market has been conducted. The objective of this research was to find out and explain profiles of business strength, business opportunities and relationships between them. This research used explanatory survey and associative descriptive method. Research objects were 5 (five) bakery business units with target population of 41 persons. Samples or informants were taken using 50% of cluster sampling or 20 informants. Data were analyzed descriptively and associatively.To be seen from internal environment, the profiles of small industries were in sufficiently strong position. The business strength lied on funding, production, and, marketing. Their weaknesses were in employments. To be seen from employment dimension, all factors still showed weaknesses. The weaknesses lied on entrepreneur ship skills/employments, employees’ skills, working conditions, and opportunities to develop for employees. From external environment profile, the business opportunities of small industries was in sufficiently good position. The good business opportunity was marketing. There were many challenges in funding, production, and employment. In funding dimension, business challenge was in providing grant, using credit, and assistance and education. In production dimension, the business challenges were aids for production infrastructures, suppliers’ abilities, and assistances and educations. In employment dimension, business challenges were in educating employments’ skills and roles of the government/third parties.The SWOT dimension analysis showed that the relationship of profile of strength and opportunity in small industries was in S-O position (strength-opportunity), or in other word, sufficient strength and sufficient opportunity. It indicated that position of small industries to be seen from internal environment was sufficiently strong (S) and from external environment was sufficiently having opportunity (O) in the market. With S-O small industry position, the possible business strategy to do was S versus O. It means that the small industries should keep/improve the existing business strengths and opportunities in the market.
UJI PENGGABUNGAN PGPF DAN Pseudomonas putida STRAIN PF-20 DALAM PENGENDALIAN HAYATI PENYAKIT BUSUK LUNAK LIDAH BUAYA DI TANAH GAMBUT Supriyanto, .; Priyatmojo, Achmadi; Arwiyanto, Triwidodo
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 11, No 1 (2011): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.11111-21

Abstract

Aloe (Aloe vera L. Webb) planted in West Borneo peat land is well known to have the best product quality in Indonesia. Bacterial soft rot is one of the constraints on aloe cultivation on peat land.  Many methods have not given significant result for controlling this disease.  The research objectives were to study the application of PGPF and its combination with Pseudomonas putida Pf-20 for controlling aloe bacterial soft rot on peat land.  In vitro test showed that two isolates of PGPF tested had different respons when combining with P. putida Pf-20.  The bacterial P. putida Pf-20 inhibit the growth of PGPF SNTH003 (Aspergillus sp.) as 20.14% on King’s B and 7.48% on PDA, whereas SNTH001 (Penicillium sp.) as 62.4% on King’s B dan 34.39% on PDA.  Glass house trial showed that root dipping in P. putida Pf-20 suspension could not promote the growth of aloe, but could reduce the disease intensity.  The single application of SNTH001 and SNTH003 isolates were able to increase the growth of aloe compare with its combination each of PGPF isolates with P. putida Pf-20.  Single application of SNTH003, SNTH001 and P. putida Pf-20 was able to reduce the disease intensity of bacterial soft rot, but the capability decreased when each of PGPF combined with P. putida Pf-20.  However combination between SNTH003 and P. putida Pf-20 increased that disease intensity. The lowest disease intensty (25%) obtained by using  SNTH001 isolate.
EFEKTIVITAS MERKURI KLORIDA (HgCl2) PADA STERILISASI TUNAS SAMPING JATI (Tectona grandis) IN VITRO Fauzan, Yusuf Sigit Ahmad; Supriyanto, .; Tajuddin, Teuku
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 4, No 2 (2017): December 2017
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v4i2.2540

Abstract

Effectiveness of Mercury Chloride (HgCl2) in Sterilization of Teak (Tectona grandis L.f.) In VitroThe main obstacle in obtaining sterile materials in in vitro cultures derived from meristems is high level of surface contamination caused by fungi and bacteria, which often results in explant death. The objective of this study was to obtain an appropriate mercury chloride (HgCl2) concentration for the sterilization of Tectona grandis nodes in in vitro culture. One cm long-sized nodes with 0.2 mm diameter were immersed in HgCl2at concentrations of 0, 100, 200 and 300 mg/L for 3 minutes. The results showed that the higher concentration of HgCl2was able to suppress the growth of fungi and bacteria and increased the percentage of aseptic explants. The best HgCl2concentration was 300 mg/L since it suppressed the growth of fungi and bacteria up to 100% and 75%, respectively, and produced the highest aseptic explants of 85% at 9 days after treatment. The small sized explants supported the sterilization process and reduced browning levels.Keywords: Browning, HgCl2, in vitro, sterilization, T. grandisABSTRAKKendala utama dalam mendapatkan material steril pada kultur in vitro yang berasal dari meristem adalah tingginya tingkat kontaminasi permukaan yang disebabkan oleh jamur dan bakteri, dan sering menyebabkan kematian eksplan. Tujuan penelitian ini adalah untuk memperoleh konsentrasi merkuri klorida (HgCl2) yang tepat untuk sterilisasi eksplan tunas samping tanaman jati (Tectona grandis) pada kultur in vitro. Tunas samping berukuran 1 cm dan diameter 0,2 mm direndam dalam HgCl2 pada konsentrasi 0, 100, 200 dan 300 mg/L selama 3 menit. Hasil penelitian menunjukkan bahwa penambahan konsentrasi HgCl2 yang semakin tinggi mampu menekan pertumbuhan jamur dan bakteri pada eksplan serta meningkatkan persentase eksplan aseptik. HgCl2 dengan konsentrasi 300 mg/L merupakan konsentrasi terbaik karena dapat menekan pertumbuhan jamur hingga 100% dan bakteri mencapai 75%, serta menghasilkan tingkat eksplan aseptik dan hidup tertinggi yaitu sebesar 85% pada 9 hari setelah perlakuan. Ukuran eksplan yang kecil mendukung proses sterilisasi dan mengurangi tingkat browning. Kata kunci: HgCl2,in vitro, pencoklatan jaringan, sterilisasi, T. grandis, Received: 02 November 2017                 Accepted: 14 December 2017                Published: 29 December 2017
Pengaruh Kombinasi Pemangkasan Akar dan Sumber Inokulum Ektomikoriza Terhadap Pertumbuhan Bibit Melinjo (Gnetum gnemon) Wulandari, Arum Sekar; Supriyanto, .; Febrianingrum, Hannum Wulan
Jurnal Ilmu Pertanian Indonesia Vol 20, No 3 (2015): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.20.3.236

Abstract

Root pruning technique may improve new lateral growth. The purposes of this research are to analyse the combined effect of root pruning and inoculum ectomycorrizal types on the growth of gnetum seedling (Gnetum gnemon). This research using split plot design. The source of ectomycorrizhal inoculum as main plot consist of 3 types: control, infected seedling of ectomycorrhizal, and soil inoculum. The root pruning level as sub plot also consist of 3 types: 0, 30, and 50%. Observation is conducted on month 5th and 6th after treatment. The result of this research indicates that root pruning technique on 7 month seedling able to enhance melinjo seedling?s growth and increase ectomycorrhizal colonization (Scleroderma spp.) after 6 months observation. The source of inoculum was able to enhance ectomyrorrhizal colonization but had no significant to gnetum seedling?s growth on month 6th after treatment. Interaction between root pruning 30% and fungal inoculation with soil inoculum source can improve gnetum seedling growth.
PENGGUNAAN BAP DAN TDZ UNTUK PERBANYAKAN TANAMAN GAHARU (Aquilaria malaccensis Lamk.) (The Use of BAP and TDZ for Propagation of Agarwood (Aquilaria malaccensis Lamk.)) Azwin, .; Siregar, Iskandar Z.; Supriyanto, .
Media Konservasi Vol 11, No 3 (2006): Media Konservasi
Publisher : Deparement of Forest Resources Conservation and Ecotourism - Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/medkon.11.3.%p

Abstract

Agarwood (A. malaccensis Lamk.) is one of the important tropical forest trees, which produces a high economically valuable fragrant resinous wood. The increase of agarwood demand from year to year leads to uncontrolled illegal harvest of this plant from its natural habitat. To encounter this problem, there is an urgent need to develop agarwood plantation. Tissue culture is an alternative method to provide genetically good seedlings for plantation in the future due to its short period and mass quantity of planlet production. In addition, through this method, its might also provide homogenous plant, and free pest and diseases. The objectives of the study were (1) to find out the optimal concentration of BAP or TDZ for inducing shoot multiplication of agarwood in in vitro conditions. MS (Murashige And Skoog, 1962), was used as basal media. The experimental design of the research was completely randomized design (RAL) with treatment of BAP concentration (control; 0,50 ppm; 0,75 ppm; 1,0 ppm) or TDZ concentration (control; 0,25 ppm; 0,50 ppm; 0,75 ppm), in 3 units, of replicate every units consist of 4 bottles, every bottle containing one explants coming from axillaries and adventitious shoot explants. Results indicated that two types of agarwood explants grown in vitro in MS basal media containing BAP 0,50 ppm or TDZ 0,25 ppm produced the highest number of shoots and leaves of agarwood plantlets, as well as its plantlet shoot length.Keywords : BAP , TDZ , Agarwood, In Vitro.
Teknik Pangkas Akar untuk Meningkatkan Produksi Bibit Melinjo Bermikoriza Wulandari, Arum Sekar; Supriyanto, .
Jurnal Ilmu Pertanian Indonesia Vol 18, No 3 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mycorrhizal symbiosis is the result of mutualistic interactions between plants and fungi. Melinjo (Gnetum gnemon) naturally associate with ectomycorrhizal Scleroderma sinnamariense and Scleroderma sp. fungi. Artificial inoculation can be applied to young seedlings (1-2 months old), before the roots contain woody material. The purpose of this study was to apply the root pruning techniques to improve gnetum mycorrhizal seedlings production. The study was conducted in a greenhouse using 7 months old seedlings. The treatments consisted of 3 root pruning levels (0, 30, and 50%) and ectomycorrhizal fungi inoculum (control, mycorrhizal seedlings, and soil inoculum). Observations were carried out for 4 months to the seedling growth and root colonization by ectomycorrhizal fungi. Height, diameter, and biomass of melinjo seedlings in all treatments were not significantly different. Thus, root pruning activities do not affect the growth of seedlings. Artificial inoculation produce mycorrhizal seedlings, whereas controls were uninfected. Root pruning increased number of root branching. Root pruning at level 50% increased mycorrhizal seedlings production, but the percentage of root colonization was not significantly difefernt at all pruning levels.