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MIX CULTURE INOCULANT PRODUCTION OF PHOSPHATE SOLUBILYZING AND INDOLE ACETIC ACID (IAA) PRODUCER RHIZOBACTERIA WITH AMBARAWA PEAT SOIL RAWAPENING AS CARRIER

JURNAL SAINS DAN MATEMATIKA Volume 18 Issue 2 Year 2010
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

ABSTRACT---Plant production development  is the main goals  that do for increase the farming quality to fulfill the man needed  in  food. One of the ways is intensive farming, by using organic or inorganic  fertilizer. Phosphate is the essential  for plants. IAA is the necessary plant regulator for the root. Both phosphate  and IAA need in  plant  growing  and production.  Biological  fertilizer  is  fertilizer  with  microbial  as the main material. Bacillus sp.DUCC-BR-K1.7, Bacillus sp. DUCC-BR-KI3, Bacillus sp.DUCC-BR-KI.2b  and Pseudomonas fluorescens, Azotobacter chroococcum, Azotobqcter vinelandii, Azospirillum sp. and Azotobacter brazilensis are bacteria that can solubilization  the phosphate  and IAA  synthesize.  Those bacteria can be used as  inoculants or biological  fertilizer  that put on carrier. One way to support  the aim is giving  the altemative carrier with  suitable composition. The carrier should be support bacterial life  during the storage.  The aim ofthis  research  is find  ths right  consortia so can be used to optimized  viabilities  of  culture Bacillus  sp. DUCC-BR-KIJ,  Bacillus sp. DUCC-BR-K1.3, Bacillus sp. DUCC-BR-KI.2b, P. flourescens, A. chroococcum, A. vinelandii, Azospirillum  sp., and  A. brazilensis in mix  culture  on peat soil  as carrier. This research done in Microbiology Laboratory of Biology Department Diponegoro University. Subculture and activated culture in Nutrient  Broth medium, make the growth curve to the biomass production, make the inoculums, prepare the peat soil, biomass production and mixed biomass with  the carrier, enumeration bacterial culture viability  test in carrier during the storage  by TPC method.  The  results  show  that  all  consortia  culture  bacteria viabiliry Bacillus  sp. DUCC-BR-KI.3  with Azospirillum (A) and Bacillus  sp. DUCC-BR-K1.7, with P.  flourescens (B)  still viable and increasing number of population during seven weeks storage with  l0 t-n CFU/g at To and up to  l0 r3-ro CFU/g at  the end storage. The  consortium  C  between Bacillus  sp.  DUCC-BR-KI.2  b  and A-  brssilensis  increasing  the  number  of population  and  still viable during eight weeks  storage  (1013  - 1014  CFU/g).Key words: biofertilizer, peat soil, viability,  mix cultare, consortiumPermalink : http://ejournal.undip.ac.id/index.php/sm/article/view/3138

Biodegradasi Senyawa Hidrokarbon Oleh Strain Bacillus cereus(VIC) Pada Kondisi Salinitas Yang Berbeda

Jurnal Akademika Biologi Vol. 4 No. 3 Juli 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bacillus cereus has been noted as hydrocarbonoclastic microbe that has ability to degrade hydrocarbons in non-saline conditions and some often to be found on high salinity environtment conditions. The purpose of this research is to determine the ability of strains Bacillus cereus (VIC) were isolated from non-saline environment to degrade hydrocarbons in crude oil on the different salinity condition. Bacillus cereus (VIC) was inoculated on the medium that has contaminated by crude oil with salinity level of 0,3 , 9,4 , dan 19,6 and incubated for 15 days. Determination of microbial growth is by using Total Plate Count (TPC) method along with determination of Total Petroleum Hydrocarbon (TPH) percentage using gravimetry method for every 5 day. The determination of microbial growth showed that Bacillus cereus (VIC) has the tolerancefor salinity level up to 19,6 because it is able to grow to a density of 6.9 x 106 CFU/ml on the 15th day. Bacillus cereus (VIC) is also able to degrades hydrocarbons on crude oil pollutants that indicated from degradation of TPH percentage in the medium up to 21% during 15 days incubation period on the medium with salinity level of 19,6 . Biodegradation using Bacillus cereus (VIC) can increase TPH degradation on the medium up to 19,8% than TPH degradation because of weathering. Keywords: Biodegradation, Bacillus cereus(VIC), Total Petroleum Hydrocarbon (TPH), Salinity.

AKTIVITAS INHIBITOR Α-AMILASE ISOLAT KHAMIR ENDOFIT DARI TUMBUHAN BROTOWALI (Tinospora crispa L.)

Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Diabetes mellitus is a carbohydrate, fat, and protein metabolism disorder characterized by high blood sugar level. The α-amylase inhibitor may inhibit the carbohydrates digestion and cause a rate decrease of glucose absorption. The α-amylase inhibitors are found in a plant that potentially containing antidiabetic compounds, such as Brotowali (Tinospora crispa L.). The exploration of endophytic microbial is expected can produce secondary metabolites with similar properties to those produced by host plant metabolites. The purpose of this research were to obtain endophytic yeast isolates which can produce the best α-amylase inhibitors and to determine the ability of selected isolates to produce α-amylase inhibitors on varied carbon source and pH production media. This research was conducted by isolating endophytic yeast from roots, stems, and leaves of brotowali. The best isolate was selected by considering its inhibitory activity. The α-amylase inhibitor test was determined by measuring the resulting reduction sugar. This research used Completely Randomised Design. First treatment was carbon source media, such as starch, sucrose, maltose and lactose. The best carbon source then was used for further tests. Second treatment was pH media including pH of 5, 6, 7 and 8. The data obtained later were analyzed using One Way ANOVA. The selection results showed that  DG26 isolate had the best α-amylase inhibition value of 68.27% with 72 hours optimal time production. The statistical result test on the varied carbon source treatment showed insignificant effect (P> 0,05) with the best α-amylase inhibition value of 19,98% at starch treatment. The statistical result test on the varied pH treatment showed a significant different effect (P <0.05) with the best α-amylase inhibition value of 44.91% at pH 7.Keywords: α-amylase inhibitor, endophytic yeast, Tinospora crispa, carbon source, pH

ISOLASI BAKTERIOFAG SPESIFIK Pseudomonas sp. DA1 DARI BIOFILM PADA SISTEM PENGISIAN AIR MINUM ISI ULANG.

Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The social demand for drinking water increases both in urban and rural areas. The state encourages the development of Industrial Water Supply Depot (DAM) refills. problems occurred is no standard method for treating drinking water is sterileand lack of government supervision. This raises the issue of sanitation that is the presence of pathogenic bacteria that form biofilms in drinking water refill system. One of the bacterial pathogen is Pseudomonas. Bacteria Pseudomonas at refill drinking water can cause infections in the digestive tract. This study aims to find specific bacteriophage isolates of biofilm samples to infect bacteria Pseudomonas sp. on refill drinking water system. Isolation host Pseudomonas done in 2 ways dilution and filtration and grown on selective media Pseudomonas Isolation Agar. Isolates obtained were then characterized by gram staining and Kligler Iron Agar (KIA) test. Isolation of bacteriophages of biofilm done by bacteriophage amplification and filtration to obtain filtrate bacteriophage I and II. Test performed by using the host's infection Pseudomonas sp. DA1, Pseudomonas aeruginosa and Salmonella sp. (LIPI’s collection). The results showed that the drinking water refill depot contained positive isolates of Pseudomonas is Pseudomonas sp. DA1 and obtained isolate specific bakteriofag that can infect Pseudomonas sp. DA1. Bacteriophage titer calculation results on each sample at 3,0 x 105 PFU/ml in drinking water depot, 3,3 x 107 PFU/ml in the water product, and 9 x 107 PFU/ml water sources. Keyword: refill drinking water systems, biofilms, Pseudomonas sp DA1, bacteriophage

EKSPLORASI DAN KARAKTERISASI BAKTERI POTENSIAL PENGHASIL SENYAWA ANTIFOULING YANG BERASOSIASI DENGAN ALGA COKLAT (PHAEOPHYTA) DI PERAIRAN KEPULAUAN KARIMUNJAWA JEPARA

Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Biofouling is a settlement process from marine organisms initiated by biofilm (microbial layers). Corrosion on the vessels, pipelines under the sea, oil plants, etc are big problems in the industrial today cause adversely affects. Antifouling paints have been developed to prevent settlement of organisms, however antifouling paints contain heavy metals and biocides which have toxic effects on marine ecosystems. Antifouling compound which environmental friendly is a solution for one of the paint component. The aim of this research was to isolate bacteria associated with brown algae which had potential to produce antifouling compound from Karimunjawa islands, Jepara.Isolate KS1-1 showed antifouling activity against Pseudomonas aeruginosa as biofilm-forming bacteria.This isolate had been characterized in morphology, moleculer and biochemichal identification.Moleculer analyses of 16S rRNA sequence, KS1-1 showed similarity 94% from 287 bp with Vibrio sp. W-137-16S ribosomal RNA gene, partial sequence. Keywords : Antifouling, Phaeophyta,Biofouling, 16S rRNA, Vibrio

OPTIMASI PEMBERIAN PUPUK GRAMAFIX DALAM DEGRADASI CEMARAN MINYAK BUMI OLEH BAKTERI INDIGENOUS SECARA IN VITRO

Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Petroleum pollution has bad impact for life in marine ecosystems, particularly in coastal areas. One method of handling the pollution is bioremediation using bacteria that are able to degrade and utilize petroleum hydrocarbons as carbon source. One technique of bioremediation is biostimulation, that is the addition of nutrients that can improve the process of oil degradation by degrading bacteria. Nutrient slow-release fertilizer are used, one of which is Gramafix. The purpose of this study was to determine the optimal concentration of Gramafix in increasing degradation of petroleum contaminants by indigenous bacteria. Four concentrations of Gramafix as treatments are P1 (0,085 g), P2 (0,171 g), P3 (0,341 g) and P4 (0,682 g), as well as the negative control treatment (no fertilizer and bacteria) and positive control (with bacteria, without fertilizer). Observations were carried out  four times, on 0, 7, 14 and 28 days of incubation. The parameters used are heavy oil using the gravimetric method, the total number of bacterial cells using Acridine Orange Direct Counting and environmental factors such as nitrogen contents, phosphorus contents, temperature, dissolved oxygen, pH and salinity. Analysis of the data using a completely randomized design (CRD) with ANOVA and Duncan test. The results showed the P3 can improve the process of oil degradation by bacteria that degrade the highest percentage of 65,91% in the 28 days of incubation, it also has more bacterial cells than other treatments. The result of this study is the addition of 0,341 g Gramafix is optimum for enhancing the degradation of petroleum hydrocarbons by degrading bacteria. Keywords: Pollution, petroleum, bioremediation, biostimulation, slow-release fertilizer.

ISOLASI DAN KARAKTERISASI BAKTERI PENDEGRADASI PESTISIDA DICOFOL DARI TANAH SAWAH DI KABUPATEN KARAWANG

Jurnal Akademika Biologi Vol. 1 No. 1 Oktober 2012
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Karawang was the center of rice production, but rice production in paddy fields in Karawawang often faces obstacle hopper pest that caused crop failure. These condition prompted the farmers to using dicofol pesticides excessively, thus caused pollution of pesticides. The purpose of this research were get the isolates bacteria that could degrade the pesticide dicofol in Karawang paddy fields and discovered characteristics of bacteria that have potential to degrade pesticides. The research was conducted with the isolation and characterisation of bacteria from Karawang paddy soil, the characterisation of isolates bacteria by observation morphological and phenotypic include staining Gram and biochemical tests, test isolates bacteria resistence of dicofol, measuring the growth curve and High Performance Liquid Chromatography (HPLC) for discovering of decreased dicofol concentration during 24, 48, and 72 hours of incubation time. The result is six isolates, that are AA1, AA2, AA3, AA4, AA5, and AA6. Isolates AA1 is suspected as genus Phenylobacterium, isolates AA3 and AA6 are suspected as genus Pseudomonas, AA4 isolates is suspected as genus Bacillus, isolates AA5 is suspected as genus Alcaligenes, but isolates AA2 not yet be identified. The results indicate that isolate AA6 has most resistent to dicofol and most number of cells bacteria, that is 7.9x108. Isolate AA6 is also the most effective in degrading dicofol as much as it can reduce the concentration in amount of 76.0 ppm (84.45%) in MSM medium + 90 ppm dicofol. Keywords : Isolation, Characterisation, Bacteria, Resistention, Degradation, Dicofol Pesticide

EKSPLORASI RHIZOBAKTERI INDIGENOUS TANAMAN CABAI RAWIT (Capsicum frustescens Linn.) DARI PERTANIAN SEMI ORGANIK DESA BATUR KABUPATEN SEMARANG SEBAGAI AGEN HAYATI PENGENDALI PERTUMBUHAN JAMUR Fusarium oxysporum f.sp capsici

Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Rhizobacteria is a group of bacteria that live in the around of plant roots. This type of bacteria known have the ability to stimulate plant growth by producing growth hormone, as well as to inhibit the growth of plant pathogens synthesize compounds with antibiotics or extracellular enzymes. The purpose of this study was to obtain and describe the morphological, biochemical and genetic isolates rhizobacteria of semi-organic farmland Semarang District village Batur which has the ability as a biological control agent of fungal pathogens Fusarium oxysporum f.sp capsici. The results obtained fifteen isolates the majority rhizobacteria bacilus shaped gram-positive and classified. Rhizobacteria inhibition test capabilities against pathogenic fungi was performed using dual culture test and test biomass. Dual culture test results showed that the inhibition of isolates E1 has a 3.77%, 1.88% isolates E3 and E15 isolates 22%. The biomass tests show E15 isolates capable of inhibiting the growth of pathogenic fungi best with the smallest weight of fungal biomass 0.0386 grams. The results of the molecular characterization based on 16S rRNA gene sequences to known that E15 isolates has similar with Bacillus cereus strain ATCC 14579 with similarity of 97%. The results of the biochemical characterization of isolates E15 has similarities with B. cereus species that is catalase positive, motile, have endospores, is able to hydrolyze starch and ferment glucose.Keywords: rhizobacteria, Fusarium oxysporum f.sp capsici, gen 16S rRNA, Bacillus cereus

KEANEKARAGAMAN DAN AKTIVITAS ENZIMATIS KAPANG RIZOSFER KACANG MEONGAN (Aeschynomene americana L.) DI DESA SUKOLILO BARAT, KECAMATAN LABANG, KABUPATEN BANGKALAN, MADURA

Jurnal Akademika Biologi Vol. 2 No. 3 Juli 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Molds are widely distributed in nature, they even present in extreme environment, such as hot and dry soil. Molds which can grow in extreme environment has been adapted to xeric environment by producing enzymes  with  special  characteristics.  The  aim  of  this  study  to  determine  the  diversity  of  molds  from A.americana L. rhizosphere at West Sukolilo Village, Labang district, Bangkalan Regency, Madura and also to examined their cellulolytic and proteolytic activities. Isolation of molds were done by using spread and dilution methods. Molds identification were done by macroscopic and microscopic examined. The molds diversity was calculated using Shannon-Wiener index. Semiquantitative enzyme examination were done using Carboxymethil Cellulose (CMC) agar for cellulolytic ang liquid gelatin 12% for proteolytic. Results of this study showed that mold diversity isolated from   A. americana L. rhizosphere was moderate (1,8-2,7) with total of 43 species, comes from 7 genus i.e. Aspergillus, Penicillium, Fusarium, Aureobasidium, Byssochlamys, Paecilomyces, and Trichoderma.  The  highest  index  of  cellulolytic  produced  by  Aspergillus  sydowii  (2,35),  while  highest proteolytic activity produced by Aspergillus flavus (86%). Keywords : molds, diversity index,xeric, cellulolytic, proteolytic

PRODUKSI INULINASE OLEH KHAMIR Pichia manshurica DUCC Y-015 PADA TEPUNG UMBI DAHLIA (Dahlia variabilis Willd.) DENGAN VARIASI KONSENTRASI MnSO4.H2O DAN WAKTU INKUBASI

Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Inulinase (E.C. 3.2.1.7) is an enzyme which able to hydrolyze an inulin into fructose and fructooligosaccharides.  The main application of inulinase enzyme in the food industries is as the ingredient in producing High Fructose Syrup (HFS). Moreover, the other important applications of inulinase enzyme are to produce ethanol, inulooligosacarida (IOS), fruktooligosacarida (FOS), pullulan, sorbitol, etc.  Pichia manshurica DUCC Y-015 is kind of yeast that is capable in producing inulinase in medium containing inulin. Optimization of inulinase enzyme production needs to be done to increase inulinase production, the way that could be conducted is by the addition of metal ion and optimization of incubation time. The purpose of this research is to investigate the effect of adding mangan ion (MnSO4.­H2O) and incubation time. In conducting this study, the researcher applies experimentally research by using Randomized Complete Block Design (RCBD) factorial pattern. The first factor is concentration of MnSO4.H2O, with concentration level 0 mM (M0), 0,1 mM (M1), 0,5 mM (M2). The second factor is the variation of incubation time, i.e. 6 hours (I6), 12 hours (I12), and 18 hours (I18) with three times repetition. The collected data were analyzed using ANOVA 5% signification (α= 0,05) and completed by the Duncan test. The result of analysis shows that variation of MnSO4.H2O concentration and incubation time does not significantly influential on inulinase activity of Pichia manshurica DUCC Y-015.Keywords: Inulinase, Inulin, Pichia manshurica DUCC Y-015, MnSO4.H2O, Incubation time.