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Phospholipids Effect on Survival and Molting Synchronicity of Larvae Mud Crab Scylla serrata SUPRAYUDI, MUHAMMAD AGUS; TAKEUCHI, TOSHIO; HAMASAKI, KATSUYUKI
HAYATI Journal of Biosciences Vol 19, No 4 (2012): December 2012
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Effect of phospholipids on survival and molting synchronicity of mud crab larvae Scylla serrata were examined using Artemia enriched with five treatments of emulsion oil i.e. treatment with different level of soybean lecithin (SL) together with a level of DHA70G (referred to as DHA-SL0, 20 and 40) and treatment with SL and cuttle fish phospholipids (CPL) at 40 uL/L without DHA70G (referred to as WDHA-SL and WDHA-CPL). Survival rate, intermolt period, carapace width, and molting synchronicity were evaluated. Additionally, lipid classes and fatty acid composition of enriched Artemia were analyzed. Survival rate, intermolt period, and carapace width at the first crab (FC) stage of mud crab larvae fed DHA-SL0 to 40 were similar to that of WDHA-CPL but higher than that of WDHA-SL (P < 0.05). Moreover, mud crab larvae fed DHA-SL20, DHA-SL40, and WDHA-CPL had a significantly higher molting synchronicity index compared to that of larvae fed WDHA-SL and DHA-SL0. It can be concluded that combination of phospholipids and essential fatty acids exhibited an additive effect in improving molting synchronicity of mud crab larvae.
Cholesterol Effect on Survival and Development of Larval Mud Crab Scylla serrata SUPRAYUDI, MUHAMMAD AGUS; TAKEUCHI, TOSHIO; HAMASAKI, KATSUYUKI
HAYATI Journal of Biosciences Vol 19, No 1 (2012): March 2012
Publisher : Bogor Agricultural University, Indonesia

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Abstract

The effect of cholesterol on the survival and development of larval mud crab Scylla serrata were examined by feeding larvae with Artemia enriched with different level of cholesterol. Artemia enriched with four stated levels of cholesterol i.e., 0, 5, 10, and 20 ul/l (Chol 0, 5, 10, and 20). All treatments were mixed with DHA70G at 25 ul/l.  All the oil was adjusted to 100 ul/l by adding the oleic acid. Survival rate, intermolt period, and carapace width at the fisrt crab stage of mud crab larvae fed Chol 0, 5, and 10 were higher compared to that of Chol 20 (P < 0.05). We suggest that free sterol contained in Artemia at 1.37% was harmful to the growth performance of mud crab larvae. This study suggests that mud crab larvae required at least 0.61% cholesterol for maintaining good survival and development and therefore no need to enrich Artemia by cholesterol for the practical purpose.
Effect of Kind and Dosage of Enrichment Materials on the Nutritional Quality of Rotifers Especially n3-HUFA Suprayudi, Muhammad Agus
Jurnal Akuakultur Indonesia Vol 2, No 1 (2003): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

This experiment was conducted to evaluate the quality of rotifer enriched with four  kinds of enrichement materials from the stand point of essensial fatty acids. Rotifer was enriched at 24 - 25oC for 18 hours at a density of 1000 ind/ml.  Rotifers were treated by four kinds of enrichment materials such as oleic acid (R-OA), two different density of Nannochloropsis oculata, (4x107 and 16x107 cell/ml; R-N18 and R-N42) two different levels of eicosapentaenoic acids (EPA) triglyceride type (EPA-TAG) (20 and 40 ml/ml; R-E20, R-E40) and two different level of EPA ethyl ester (EPA-EE) (R-EE25 and R-EE50%) respectively. Rotifers enriched with Nannochloropsis oculata and EPA-EE type have a similar profile of essensial fatty acid especially on n3-HUFA that dominated by EPA, while DHA was in a trace amount or not detected.  In addition Nannochloropsis oculata as an enrichment material showed the highest population density of rotifers during enrichment periods.  Rotifer enriched with EPA-TAG has a more complete of essential fatty acid profile compared to other enrichment materials due to their contained both of EPA and DHA. We conclude that rotifer enriched with EPA-TAG as enrichment material showed the best nutritional quality of rotifers from the stand point of essential fatty acid. Key words :  Rotifers, enrichment, eicosapentaenoic acid, docosaheksaenoic acid,  n3-HUFA   ABSTRAK Penelitian ini dilakukan untuk mengetahui profil asam lemak rotifera yang diperkaya dengan berbagai macam jenis dan dosis pengkaya. Rotifera dengan kepadatan 1000 ind./ml diperkaya dengan bebagai bahan pengkaya seperti asam oleat (R-OA); Nannochloropsis oculata dengan kepadatan 4x107 dan 16x107 sel/ml (R-N18 dan R-N42); eicosapentaenoic acid (EPA) tipe triglicerida (EPA-TAG)  dengan dosis 20 dan 40 μl (R-E20 dan R-E40) dan EPA tipe ethyl esther (EPA-EE)  dengan dosis 25 dan 50 μl per liter (R-EE25 and R-EE50%). Rotifera diperkaya selama 18 jam pada suhu 24-25oC. Rotifera yang diperkaya dengan Nannochloropsis oculata serta EPA-EE memiliki kesamaan profil asam lemak terutama pada incorporasi EPA, sedangkan DHA terkandung pada jumlah yang kecil atau tidak terdeteksi.  Adapun rotifera yang diperkaya dengan EPA-TAG memiliki profil asam lemak yang lebih lengkap terutama ditinjau dari kandunga EPA dan docosahexaenoic acid (DHA) nya.  Selanjutnya rotifera yang diperkaya dengan Nannochloropsis oculata memiliki populasi yang tertinggi dibanding perlakuan lainnya. Dari penelitian ini dapat disimpulkan bahwa pengkayaan dengan EPA-TAG memiliki kualitas rotifera yang terbaik ditinjau dari sisi kelengkapan kandungan asam lemaknya. Kata kunci :  Kualitas rotifera, pengkayaan, asam eikosapentaenoik, asam dokosaheksaenoik, n3-HUFA
Free Amino Acid Enriched Rotifer for Larval Grouper Cromileptes altivelis (Pengkayaan Rotifer dengan Asam Amino Bebas Untuk Larva Kerapu Bebek Cromileptes altivelis) Jusadi, Dedi; Aprilia, Tulas; Suprayudi, Muhammad Agus; Yaniharto, Deddy
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 4 (2015): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Pembenihan ikan kerapu dihadapkan pada rendahnya sintasan, terutama fase awal hidupnya. Rendahnya sintasan diduga berhubungan dengan belum berkembangnya organ pencernaan larva dan tidak tersedianya pakan yang siap diserap tubuh. Pemberian asam amino bebas seperti taurin dan glutamin melalui rotifer diharapkan dapat meningkatan ketersediaan nutrien yang dapat diserap dengan cepat oleh larva, sehingga sintasan larva tinggi dan dapat tumbuh lebih cepat. Penelitian bertujuan mengukur pengaruh glutamin atau taurin terhadap sintasan dan pertumbuhan larva kerapu bebek Cromileptes altivelis. Larva umur 1 hari (10 ekor.L-1) dipelihara dalam bak fiberglass volume 500 L yang diisi air laut sampai 330 L. Larva diberi pakan rotifer diperkaya 0,5 g glutamin, 0,5 g taurin, atau tanpa glutamin dan taurin. Larva dipelihara selama 16 hari dengan pemberian pakan 2 kali? per hari. Satu jam setelah pemberian pakan dilakukan pengamatan terhadap jumlah rotifer dalam saluran pencernaan larva ikan. Hasil penelitian menunjukkan jumlah rotifer dalam saluran pencernaan larva meningkat seiring dengan bertambahnya umur. Namun, jumlah rata-rata rotifer yang dikonsumsi larva pada perlakuan 0,5 g glutamin atau 0,5 g taurin lebih banyak dibanding perlakuan tanpa taurin dan glutamin. Larva yang diberi rotifer hasil pengkayaan dengan 0,5 g glutamin atau 0,5 g taurin memiliki sintasan yang tidak berbeda (P>0,05), masing-masing 19,2?2,25%, dan 24,5?1,20%, dan secara signifikan lebih banyak dibanding larva di perlakuan tanpa glutamin dan taurin yang nilainya 13,0?1,70%. Larva yang diberi rotifer hasil pengkayaan dengan 0,5 g taurin secara signifikan lebih panjang (5,5?0,18 mm) dari ikan di perlakuan 0,5 g glutamin (5,3?0,12 mm). Demikian juga, larva di perlakuan glutamin lebih panjang dari pelakuan tanpa glutamin dan taurin (5,0?0,16 mm). Pengkayaan rotifer menggunakan asam amino bebas taurin atau glutamin dapat meningkatkan sintasan dan pertumbuhan larva kerapu bebek. Namun, taurin lebih baik dibanding glutamin karena memberi efek ke pertumbuhan ikan yang lebih cepat. Kata kunci: taurin, glutamin, kerapu bebek, Cromileptes altivelis. The hatchery production of grouper faced to the problem of low surival rate, especially during the first feeding period. During this period, the digestive organ of larvae was underdeveloped, and the nutrient in live food was not readily absorbed by larvae. Feeding on free amino acids, such as taurine or glutamine, enriched rotifer would be enhanced the nutrient readily absorbed thereby resulting to the improvement of growth and survival rate of larvae. A duplicate experiment was conducted to evaluate the effect of rotifer enriched with free amino acid of glutamine or taurine, on the growth and survival rate of larval grouper Cromileptes altivelis. One day old larvae were cultured in 500 l fiberglass containing 330 l of sea water with a density of 10 ind.L-1. Larvae were fed on rotifer enriched with either 0.5 g glutamine, 0.5 g taurine, or without glutamine nor taurine. Larvae were fed on rotifer twice a day at 08.00 and 14.00, and cultured for 16 days. One h after feeding, rotifer content in the gut of larvae were observed. Results showed that larvae fed on rotifer enriched with 0.5 g taurine or 0.5 g glutamie had higher rotifer content in the gut than that in the other group. Larvae fed on rotifer enriched with 0.5 g glutamine or 0.5 g taurine had the same survival rate (P>0.05), 19.2?2.25% and 24.5?1.20%, respectively; these values were significantly higher than that in the other group which was 13.0?1.70%. ?The total length of larvae fed on rotifer enriched with 0.5 g taurine (5.5?0.18 mm) significantly was the biggest compared than others; while those fed on rotifer enriched with 0.5 g glutamine (5.3?0.12 mm) was bigger than fish fed on rotifer without glutamine nor taurine (5.0?0.16 mm). Thus, it can be concluded that survival rate and growth of larvae were improved when feeding on rotifer enriched with taurine or glutamine. Nevertheless, taurine was more efective than glutamine due to its effect resulted the highest growth performance of larval grouper. Keywords: taurine, glutamine, grouper, Cromileptes altivelis
Quality evaluation of fermented products of various local agroindustrials by-products: the effect on digestibility and growth performance of common carp juvenile Suprayudi, Muhammad Agus; Edriani, Gebbie; Ekasari, Julie
Jurnal Akuakultur Indonesia Vol 11, No 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

The aim of this research was evaluating the quality and digestibility of fermented local feedstuff as well as its effect on growth performance of common carp Cyprinus carpio juvenile. The local feedstuffs tested in this experiment were kapok seed, cassava peel, copra, rubber seed, and palm kernel meal. The previously milled feedstuff was fermented with instant yeast Saccharomyces cerevisiae with a dose of 0.9% w/w and incubated for 24 hours. Following this, the fermented feedstuffs were dried, mixed with reference diet with a ratio of 3:7 and supplemented with 0.5% Cr2O3 as digestibility test indicator. Common carp juveniles with an initial average body weight of 14,11±1,28 g were cultured for 30 days. Fish feces were collected since the 6th day of culture, and followed by laboratory analyses. The results show that fermentation could increase the crude protein content of feedstuff with a range of 16.85‒31.11%, and decrease crude fiber with a range of 2.45‒31.65% with the exception of copra. Furthermore, fermentation also increased the feed digestibility including protein, energy, and total digestibility by the tested fish, as it is shown that the use of fermented feedstuffs may increase protein digestibility 3.88‒11.73%, 2,21‒10,24%, and 3,63‒72,37%. Finally, it can be concluded that fermentation can increase the digestibility of feed with local ingredients by common carp juvenile.   Keywords: fermentation, digestibility, local materials, common carp
Growth Response and Feed Utilization of Giant Gourami (Osphronemus goramy) Juvenile Feeding Different Protein Levels of the Diets Supplemented with Recombinant Growth Hormone BUDI, DARMAWAN SETIA; ALIMUDDIN, .; SUPRAYUDI, MUHAMMAD AGUS
HAYATI Journal of Biosciences Vol 22, No 1 (2015): January 2015
Publisher : Bogor Agricultural University, Indonesia

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Abstract

The purpose of this study was to examine the effect of dietary supplementation with recombinant growth hormone (rGH) on the growth and dietary utility of juvenile giant gourami. The rGH was mixed with chicken egg yolk and sprayed on to artificial feed with different protein levels (34, 28, and 21%; isoenergy). Each treatment group of gourami was paired with a control group that received feed of the same protein level, but without rGH supplementation. Juvenile of giant gourami (weight 15.83 + 0.13 g) were fed diets containing rGH, to apparent satiation, 2 times a week. Fish were reared from less than 2 months old? for 42 days in 100 L glass aquaria at an initial density of 10 fish per aquarium. At the end of this period, the biomass and daily growth rate (SGR) of the fish were measured and the feed conversion ratio calculated and compared. Our data showed that fish fed rGH-supplemented diets experienced higher growth than fish in the control groups and showed that fish with higher protein diets experienced higher growth than the groups with less protein diets. The group with the highest biomass gain, SGR, and lowest feed conversion ratio (FCR) was the group fed a 34% protein diet supplemented with rGH. Furthermore, biomass gain, SGR, and FCR in the rGH treatment group with a 28% protein diet matched the measurements of the non-rGH control group receiving a 34% protein diet (P > 0.05). We conclude that giant juvenile gourami given feed supplemented with recombinant growth hormone will experience increased growth and dietary utility compared with gourami given the same feed without supplementation.
The growth of red tilapia fed on organic-selenium supplemented diet Suprayudi, Muhammad Agus; Faisal, Burhanudin; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol 12, No 1 (2013): Jurnal Akuakultur Indonesia
Publisher : ISSA

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Abstract

ABSTRACT The experiment was conducted to evaluate the level of organic selenium in the diet on the growth performance of red tilapia Oreochromis sp. Four levels of organic selenium namely 0, 1, 2, and 4 g Se/kg diet were used as a treatment, and the selenium content on the diets are 0.12, 0.19, 1.05, and 1.42 mg Se/kg respectively. This experiment were used randomized design with four treatments and two replications. All the diet was formulated to have similar protein and energy. Juvenile red tilapia with average body weight of 9.49±0.94 g were reared in the 80×40×40 cm3 aquarium with density of 10 fish/aquarium. Fish were reared for 40 days and feed four times daily at satiation levels. The result of this study showed that fish fed diet containing 4 g organic Se/kg diet (1,42 mg Se/kg) had the best growth performance. Keywords: organic selenium, growth, red tilapia  ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh dosis selenium organik pada pakan terhadap kinerja pertumbuhan ikan nila merah Oreochromis sp. Empat macam dosis selenium organik yang digunakan adalah 0, 1, 2, dan 4 g Se/kg pakan. Kandungan selenium pada setiap pakan berturut-turut adalah 0,12; 0,19; 1,05; dan 1,42 mg Se/kg pakan. Penelitian ini menggunakan rancangan acak dengan empat perlakuan dan dua ulangan. Semua pakan diformulasikan memiliki protein dan energi yang sama. Ikan yang digunakan adalah juvenil nila merah dengan bobot rata-rata 9,49±0,95 g yang dipelihara dalam akuarium berukuran 80×40×40 cm3 dengan kepadatan 10 ekor/akuarium. Ikan tersebut dipelihara selama 40 hari dengan frekuensi pemberian pakan empat kali sehari secara at satiation. Hasil penelitian ini menunjukkan bahwa ikan yang diberi pakan yang mengandung 4 g Se organik/kg pakan (1,42 mg Se/kg) memperlihatkan kinerja pertumbuhan terbaik. Kata kunci: selenium organik, pertumbuhan, nila merah 
Feeding for larvae of catfish Pangasionodon sp. larvae in different ages Suprayudi, Muhammad Agus; Ramadhan, Ricky; Jusadi, Dedi
Jurnal Akuakultur Indonesia Vol 12, No 2 (2013): Jurnal Akuakultur Indonesia
Publisher : ISSA

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Abstract

ABSTRACT Sludge worm (Tubifex sp.) as natural feed on catfish (Pangasionodon sp.) larvae rearing is available in limited amount especially during rainy season. It becomes a constraint factor for larvae rearing sector. This research was conducted to evaluate the appropriate initial age of catfish larvae to get artificial feed as sludge worm replacement. Evaluation was conducted on the growth and survival of catfish larvae in 14 days of culture. There were four treatments of feeding in triplicates i.e. larvae were given natural feed without artificial feed, given artificial feed started from d3, d6, and d9) with three replications. The results showed that larvae fed on artificial feed on d3 had the lowest growth compared to the other treatments, whereas the survival was not significantly different (P&gt;0.05) among the treatments. As a conclusion, artificial feed could be used to replace natural feed for catfish larvae started at the age of nine days. Keywords: sludge worm, catfish larvae, artificial feed  ABSTRAK Cacing sutra (Tubifex sp.) tersedia dalam jumlah terbatas terutama pada musim penghujan sebagai pakan alami dalam usaha pembenihan ikan patin (Pangasionodon sp.). Ini menjadi kendala dalam usaha pembenihan. Penelitian ini dilakukan untuk mengevaluasi umur larva ikan patin yang tepat untuk mulai diberi pakan buatan menggantikan cacing sutra. Evaluasi dilakukan pada pertumbuhan dan kelangsungan hidup larva ikan patin umur 14 hari. Selama pemeliharaan, larva diberi pakan dengan empat perlakuan; pemberian pakan alami tanpa pakan buatan, pemberian pakan buatan mulai d3, d6, dan d9 dengan tiga ulangan untuk masing-masing perlakuan. Hasil penelitian menunjukkan bahwa perlakuan pemberian pakan buatan mulai d3 memiliki pertumbuhan panjang yang terkecil dibandingkan perlakuan lain, sedangkan tingkat kelangsungan hidup larva tidak berbeda nyata (P&gt;0,05) antarperlakuan. Berdasarkan hasil penelitian, dapat disimpulkan bahwa pakan buatan dapat digunakan sebagai pengganti pakan alami mulai larva ikan patin umur sembilan hari. Kata kunci: cacing sutra, larva ikan patin, pakan buatan
Growth and immune response of Litopenaeus vannamei fed on β-(1, 3) glucan and poly-β hydroxybutyrate Sarmin, ,; Suprayudi, Muhammad Agus; Jusadi, Dedi; Ekasari, Julie
Jurnal Akuakultur Indonesia Vol 12, No 2 (2013): Jurnal Akuakultur Indonesia
Publisher : ISSA

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Abstract

ABSTRACT This research was aimed to examine the growth performance and non-specific immune response of Pacific white shrimp (Litopenaeus vannamei) fed on the diet supplemented with β-(1,3) glucan (BG) and poly-β-hydroxybutyrate (PHB) as feed additives. Shrimp juvenile at an initial body weight of 2.06±0.03 g was randomly distributed into 12 units of aquaria at a density of 20 shrimps/tank and reared for 42 days. The treatments applied in this study were control (without feed additives), 1.5 g/kg BG, 10 g/kg PHB and 1,5 g/kg BG+10 g/kg PHB. Results showed that shrimp fed on 1.5 /kg BG-supplemented feed had significantly higher growth performance and non-specific immune response. Keywords: growth, shrimp, non-specific immune response, Litopenaeus vannamei  ABSTRAK Penelitian ini bertujuan untuk menguji kinerja pertumbuhan dan respons imun nonspesifik udang vaname Litopenaeus vannamei yang diberi pakan dengan penambahan feed additive berupa β-(1,3) glukan (BG) dan poli-β-hidroksibutirat (PHB). Juvenil udang 2,06±0,03 g dipelihara pada 12 unit akuarium dengan empat perlakuan dan tiga ulangan, serta padat tebar 20 ekor/tank selama 42 hari pemeliharaan. Perlakuan yang diberikan dalam penelitian ini yaitu penambahan BG (1,5 g/kg), PHB (10 g/kg), dan BG (1,5 g/kg)+PHB (10 g/kg), serta kontrol (tanpa penambahan feed additive). Hasil penelitian menunjukkan bahwa udang yang diberi 1,5 g/kg BG memiliki kinerja pertumbuhan dan respons imun nonspesifik yang terbaik. Kata kunci: pertumbuhan, udang, respons imun nonspesifik, Litopenaeus vannamei
Combination of Tubifex and artificial diet for catfish Pangasianodon hypophthalmus larvae Jusadi, Dedi; Anggraini, Ria Septy; Suprayudi, Muhammad Agus
Jurnal Akuakultur Indonesia Vol 14, No 1 (2015): Jurnal Akuakultur Indonesia
Publisher : ISSA

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Abstract

ABSTRACT This research was conducted to evaluate the effect of different combination of feeding frequency of Tubifex (C) and artificial diet (P) on survival and growth rate of larval yellow tail catfish Pangasianodon hypophthalmus. Newly hatched larvae (d0) with total length of 0.44±0.04 cm were cultured for 14 days in 30×20×20 cm3 aquarium. Water volume in each aquarium was 9 L. At d5, larvae were fed on five different feeding frequencies either 6C+0PB, 5C+2PB, 3C+3PB, 2C+5PB, dan 0C+6PB. Feeding method used in this research was ad libitum. Results showed that survival and total length of d14 old larvae fed on 0C+6PB was lower than the groups fed on either 6C+0PB or three combinations of C+PB. Lipase activity tend to be increase in line with the increasing amount of artificial feed. On the other hand, larvae fed on 6C+0PB and 0C+6PB had the lowest protease activity. Therefore, larval catfish can be fed by combination of Tubifex and artificial feed. Keywords: artificial diet, lipase activity, protease activity, sludge worm, Pangasianodon hypophthalmus  ABSTRAK Penelitian ini mengevaluasi pengaruh perbedaan frekuensi pemberian pakan harian cacing sutra (C) dan pakan buatan (PB) terhadap sintasan dan pertumbuhan larva ikan patin Pangasianodon hypophthalmus. Larva ikan patin yang baru menetas berukuran 0,44±0,04 cm dipelihara dalam akuarium kaca berukuran 30×20×20 cm3 yang diisi air setinggi 15 cm selama 14 hari. Mulai dh5, larva diberi lima perlakuan frekuensi pemberian pakan, yaitu 6C+0PB, 5C+2PB, 3C+3PB, 2C+5PB, dan 0C+6PB. Pemberian pakan dilakukan secara ad libitum. Hasil penelitian menunjukkan bahwa larva yang diberi 6C+0PB dan kombinasi C+PB memiliki tingkat kelangsungan hidup dan panjang akhir yang tidak berbeda nyata. Namun larva yang diberi 0C+6PB, menghasilkan tingkat kelangsungan hidup dan panjang akhirnya terendah. Semakin banyak porsi sering frekuensi pemberian PB yang diberikan menunjukkan tren aktivitas lipase yang cenderung meningkat. Tetapi pada aktivitas protease pada pemberian 6C+0PB dan 0C+6PB lebih rendah dari ketiga perlakuan kombinasi C+PB. Dengan demikian, larva ikan patin dapat dibudidaya dengan menggunakan kombinasi cacing sutra dan pakan buatan. Kata kunci: pakan buatan, aktivitas lipase, aktivitas protease, cacing sutra, Pangasianodon hypophthalmus