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EKSPRESI CYTOSOLIC ASPARTATE-SPECIFIC CYSTEINE PROTEASE-3 (CASPASE-3) PADA JARINGAN HATI RATTUS NORVEGICUS (WISTAR) SETELAH PEMBERIAN SUBKRONIK AFLATOKSIN B1 (AFB1) Maritha, Indah Dina; Supranowo, Supranowo; Lyrawati, Diana
Jurnal Kedokteran Brawijaya Vol 22, No 3 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Abstract

Aflatoksin B1(AFB1) is one of the toxic agents produced by Aspergillus flavus that frequently contaminates foods not properly stored. AFB1 undergoes biotransformation which may result in the production of reactive oxygenspecies (ROS) hazardous to liver cells. Following a cascade of oxidative reaction, ROS will cause the mitochondria torelease cytochrome c which subsequently activates caspase-3, leading to apoptosis. In this present study we evaluated the effect of aflatoxin B1 on the expression of caspase-3 in the liver. AFB1 was administered per oral, at different dosage and length of exposure, subchronically. This study was carried out as a factorial designed experiment with  two factors. The first was dosage factor i.e 0, 10, 15 and 20 µg (0; 0,05; 0,075; 0,1 µg/g BW) and the second was exposure time factor i.e. 12, 16 and 20 weeks. Rattus norvegicus strain Wistar aged approximately eight weeks old and weighed 180-200 g were used as the experimental animals. The expression of  caspase-3 was examined by using immunohistochemistry. The results showed that the expression of caspase-3 increased significantly (p = 0,000) with the escalation of AFB1 in dosage and/or exposure time (p = 0,001). In the interaction between dose and exposure time of AFB1an increase in the expression of caspase-3 was also observed (p = 0,000). Interestingly, these studies also revealed thatin the liver tissues there was a limitation in the expression of caspase-3, where the raising of further AFB1 dosage and length of exposure were not followed by further increase of the caspase-3.
Ekstrak Rosela Menurunkan Perlemakan dan Ekspresi ADMA Hepar akibat Diet Aterogenik pada Tikus Fathoni, Zuhrotun Ulya; Indra, Rasjad; Supranowo, Supranowo
Jurnal Kedokteran Brawijaya Vol 28, No 1 (2014)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (719.579 KB) | DOI: 10.21776/ub.jkb.2014.028.01.2

Abstract

Rosela memiliki anthocyanin sebagai antioksidan polifenol yang berpotensi mencegah progresivitas penyakit akibat stres oksidasi. Diet aterogenik dan stres oksidasi memicu serangan radikal bebas pada komponen lemak dan protein sehingga terjadi perlemakan hepar dan peningkatan ekspresi ADMA (Asymmetric dimethylarginine). Penelitian ini bertujuan mengetahui pengaruh pemberian ekstrak rosela strain Hibiscus sabdariffa terhadap perlemakan hepar dan ekspresi ADMA hepar pada tikus Rattus norvegicus yang diberi diet aterogenik. Studi eksperimental menggunakan post test only control group pada Rattus norvegicus strain Wistar jantan yang dibagi dalam empat kelompok, yaitu kelompok kontrol positif (diet aterogenik), kelompok1 (ekstrak rosela 150mg/kgBB/hari + diet aterogenik), kelompok 2 (ekstrak rosela 300mg/kgBB/hari + diet aterogenik), dan kelompok kontrol negatif (tanpa pemberian diet aterogenik dan ekstrak rosela). Hasil penelitian menunjukkan bahwa ekstrak rosela mampu menurunkan perlemakan hepar yang berdasar pemeriksaan histopatologi berupa jumlah sel yang mengalami perlemakan lebih sedikit (p=0,000). Ekspresi ADMA dari gambaran imunohistokimia menunjukkan ekspresi yang lebih sedikit pada kelompok dengan diberi ekstrak rosela (p=0,000). Dapat disimpulkan pemberian ekstrak rosela menghambat perlemakan hepar akibat diet aterogenik dan ekspresi ADMA akibat diet aterogenik pada tikus, dengan dosis optimum rosela 300 mg/kgBB/hari. Kata Kunci: Diet aterogenik, ekspresi ADMA hepar, perlemakan hepar, rosela
EKSPRESI CYTOSOLIC ASPARTATE-SPECIFIC CYSTEINE PROTEASE-3 (CASPASE-3) PADA JARINGAN HATI RATTUS NORVEGICUS (WISTAR) SETELAH PEMBERIAN SUBKRONIK AFLATOKSIN B1 (AFB1) Maritha, Indah Dina; Supranowo, Supranowo; Lyrawati, Diana
Jurnal Kedokteran Brawijaya Vol 22, No 3 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1838.995 KB) | DOI: 10.21776/ub.jkb.2006.022.03.4

Abstract

Aflatoksin B1(AFB1) is one of the toxic agents produced by Aspergillus flavus that frequently contaminates foods not?properly stored. AFB1 undergoes biotransformation which may result in the production of reactive oxygenspecies (ROS)?hazardous to liver cells. Following a cascade of oxidative reaction, ROS will cause the mitochondria torelease?cytochrome c which subsequently activates caspase-3, leading to apoptosis. In this present study we evaluated the effect?of aflatoxin B1 on the expression of caspase-3 in the liver. AFB1 was administered per oral, at different dosage and?length of exposure, subchronically. This study was carried out as a factorial designed experiment with ?two factors. The?first was dosage factor i.e 0, 10, 15 and 20 ?g (0; 0,05; 0,075; 0,1 ?g/g BW) and the second was exposure time factor i.e.?12, 16 and 20 weeks. Rattus norvegicus strain Wistar aged approximately eight weeks old and weighed 180-200 g were?used as the experimental animals. The expression of ?caspase-3 was examined by using immunohistochemistry. The?results showed that the expression of caspase-3 increased significantly (p = 0,000) with the escalation of AFB1 in dosage?and/or exposure time (p = 0,001). In the interaction between dose and exposure time of AFB1an increase in the?expression of caspase-3 was also observed (p = 0,000). Interestingly, these studies also revealed thatin the liver tissues?there was a limitation in the expression of caspase-3, where the raising of further AFB1 dosage and length of exposure?were not followed by further increase of the caspase-3.
ANALISIS HASIL BELAJAR DAN KEMANDIRIAN SISWA PADA PEMBELAJARAN ASAM BASA DENGAN METODE BLENDED LEARNING Niasri, Niasri; Cahyono, Edy; Supranowo, Supranowo
Jurnal Inovasi Pendidikan Kimia Vol 13, No 2 (2019): July (2019)
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Semarang

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Abstract

The development of information technology has a big impact on the world of education. Progress in information technology makes it easier to implement learning. Blended learning is one of the learning that is in accordance with these developments.This study aims to analyze the learning outcomes and autonomy of students after blended learning. The research method used is mixed methods. The design used is explanatory sequential design. The research sample was taken using purposive sampling technique, with the study sample using one class in the XI MIPA class of SMA N 1 Padamara. Data collection methods in this study include documentation, tests, observations, questionnaires and interviews. The results of the study showed that the edmodo application assisted blended learning was obtained by the percentage of classical learning completeness of 86.67%, the percentage of skill aspects was 88.40% and the student attitude aspect was 82.29%. The analysis of the autonomy of students in class XI MIPA 1 obtained a percentage of 77.27%. Based on the results of the study, it can be concluded that the learning outcomes and autonomy of students of class XI MIPA 1 in SMA N 1 Padamara on acid-base material with assisted blended learning are edmodo applications are good. This shows that assisted blended learning is an effective edmodo application on student learning outcomes and autonomy.