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Journal : Indonesian Journal on Learning and Advanced Education (IJOLAE)

The Use of Natural Dyes from Beetroot Skin Extract (Beta Vulgaris) as Teaching Material on Cell Division for Senior High School Students Azizah, Adilla Nur; Agustina, Putri; Suparti, Suparti; Saputra, Alanindra; Sidiq, Yasir
Indonesian Journal on Learning and Advanced Education (IJOLAE) Vol. 2, No. 1, January 2020
Publisher : Faculty of Teacher Training and Education, Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/ijolae.v2i1.9051

Abstract

One of the Basic Competencies (KD) in Biology for Indonesian Senior High School is KD 4.12 which reads "Presents observations of the process of mitosis in the onion root and determines the phases it finds". Based on the basic competency test, students are required to have skills in making and observing mitotic preparations of plant tissue with at least using onion. Observation of mitotic division in plant tissues requires skill in making preparations. Making preparations in observing cells and tissues of plants or animals needs staining. Dyes often used in lab work are synthetic dyes. One of the synthetic dyes commonly used is safranin. However, safranin is quite expensive. This limitation requires teachers to look for alternatives to synthetic dyes that are easily found at a more affordable price, namely with natural dyes. One of the plants that has the potential to overcome this issue is beetroot (Beta vulgaris). The purpose of this study was to determine the quality of plant tissue preparations (Allium cepa) stained with beetroot skin (Beta vulgaris) as a natural coloring agent. The research method used was an experimental method with a Completely Randomized Design consisting of two factors, namely the staining time (1 hour, 2 hours, and 3 hours) and the type of solvent (aquades and citric acid 10%). The results of the study were analyzed with qualitative descriptive methods which included contrast and clarity of the preparations. Based on the results of the study, the quality of Allium cepa root preparations using beetroot (Beta vulgaris) skin extract with variations in the type of solvent and duration of staining showed good results on the staining time of 1 hour and 2 hours. The staining time of the preparations can affect the contrast and clarity of the preparations.
Co-Authors A. Sulaksono, A. Abdul Hoyyi Afa, Ihdayani Banun Agus Cahyono Agus Rusgiyono Ahmad Reza Aditya Akhmad Zaki Alan Prahutama Alanindra Saputra Alvita Rachma Devi Amanda Devi Paramitha, Amanda Devi Aminah Asngad Any Setyaningsih, Any Arief Rachman Hakim Asismarta Asismarta, Asismarta Azizah, Adilla Nur Bayu Ariawan Budi Warsito Bunga Maharani, Bunga C Yuwono Sumasto, C Yuwono Deden Aditya Nanda, Deden Aditya Di Asih I Maruddani Diah Budiati Diah Safitri Dini Puspita Dwi Ispriyanti Dyah Ayu Kusumaningrum Ebeit Devita Simatupang Elyas Darmawan Ernawati, Devi Ernik Yuliana Esti Pratiwi Fajar Heru Setiawan, Fajar Heru Farikhin Farikhin Fina Fitriyana Firda Megawati, Firda Fitri Juniaty Simatupang, Fitri Juniaty Fitriyatno Fitriyatno Gita Suci Ramadhani Hafii Risalam, Hafii Hanifa Eka Oktafiani, Hanifa Eka Happy Suci Puspitasari Hasbi Yasin Icha Puspitasari Ikrima, Hanjar Indah Puspaningrum Indra Satria, Indra Iwan Ali Sofwan Izzudin Khalid, Izzudin Karimawati, Nurul Kartika, Aninda Ayu Kartikaningtiyas Hanunggraheni Saputri, Kartikaningtiyas Hanunggraheni Khoirunnisa Nur Fadhilah, Khoirunnisa Nur Lailly Rahmatika, Lailly Lina Agustina, Lina Lintang Afdianti Nurkhasanah, Lintang Afdianti Lismiyati Marfuah, Lismiyati Lulus Darwati, Lulus Ma'sum, M. Ali Moch. Abdul Mukid Muhammad Taufan Musandingmi Elok Nurul Islam, Musandingmi Elok Nurul Mustafid Mustafid Mustofa, Achmad Natanael, Dimas Kevin Natanael, Dimas Kevin Ndaru Dian Darmawanti Nonik Brilliana Primastuti Novia Agustina, Novia Onny Kartika Hitasari, Onny Kartika Paula Meilina Dwi Hapsari Putri Agustina Rahmawati, Resti Rahmawati, Rizky Dwi Rahmawati, Rizky Dwi Rambat Rambat, Rambat Ratih Binadari, Ratih Ria Sutitis, Ria Riana Ayu Andam Pradewi Richy Priyambodo Rita Rahmawati Riyan Eko Putri Rukun Santoso Sa'adah, Alfi Faridatus Sadjati, Ida Malati Sanitoria Nadeak, Sanitoria Sari, Shinta Karunia Permata Seta Satria Utama Setiawati, Teti Setya Ayu Rahmawati Siti Anisah Sofyan Anif Sri Budiasih, Sri Sri Sumiyati Sri Wahyuningrum Sudargo Sudargo, Sudargo Sudarno Sudarno Sugito Sugito Sulton Syafii Katijaya Sunardi Sunardi Swasnita Swasnita, Swasnita Syariati, Dian T. Mart, T. Tarno Tarno Tedjo, Martyanto Tiani Wahyu Utami Triastuti Rahayu Triastuti Wuryandari Triyanto Triyanto Tyas Estiningrum Umi Sulistyorini Adi, Umi Sulistyorini Victoria Dwi Murti Wasis Wicaksono Widari Widari, Widari Wulan Safitri, Wulan Yasir Sidiq Yon Haryono Yuciana Wilandari Yudi Ari Wibowo Yusuf Arifka Rahman, Yusuf Arifka Zia, Nabila Ghaida Zubaidah, Lailia