NI WAYAN SUNITI
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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PENGUJIAN DAYA HAMBAT EKSTRAK BEBERAPA TUMBUHAN TERHADAP JAMUR CERATOCYSTIS SP. PENYEBAB PENYAKIT BUSUK BERAIR PADA BUAH SALAK BALI SUNITI, NI WAYAN
AGRITROP Vol. 26, No. 2 Juni 2007
Publisher : AGRITROP

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The research aimed to determine the inhibition activity of some plant extracts against Ceratocystis sp. on PDA medium, salacca piece, whole fruit and liquid medium. Research?s result showed that among nine plant extracts, two extracts inhibited the growth of Ceratocystis effectively, namely extract of Albinia galanga rhizome and boiled papaya leaf. Albinia galanga rhizome showed inhibitory 92.5 % on PDA medium and 89.6% on PD Broth liquid medium. Boiled papaya leaf extract showed 73.3% on PDA medium and 57.7 % on PD Broth liquid medium. On fruit piece and whole fruit inhibition activity of boiled papaya leaf extract has higher than to Albinia galanga rhizome extract.
SUPPRESSION ABILITY OF CRUDE EXTRACT DERIVED FROM MARINE BIOTA AGAINST FUSARIUM OXYSPORUM F.SP. VANILLAE Suada, I Ketut; Suniti, Ni Wayan
Jurnal Biologi Vol XIV, No 1
Publisher : Jurnal Biologi

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The objective of this research was to investigate suppression ability of marine biota extracts against Fusarium oxysporum f.sp. vanillae of vanilla stem rot. Samples were collected at intertidal zones and in the depth of 1-7 m from seven beaches in Bali. Screening of active compounds of biota extracts were conducted using inhibition zone of well diffusion method on Potato Dextrose Agar (PDA). The extract was tested in-vitro in PDA medium using completely randomized design with three replicates. The methanolic extract of Aglaophenia sp. was able to suppress the growth of F. oxysporum f.sp. vanillae effectively, with minimum inhibition concentration (MIC) of 0.05 %. The extract inhibited colony growth diameter and total mycelial dry weight.
Uji Efektivitas Konsentrasi Minyak Atsiri Sereh Dapur (Cymbopogon Citratus (DC.) Stapf) terhadap Pertumbuhan Jamur Aspergillus Sp. secara In Vitro ELLA, MARIA ULFA ELLA; SUMIARTHA, KETUT SUMIARTHA; SUNITI, NI WAYAN; SUDIARTA, I PUTU; ANTARA, NYOMAN SEMADI
E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.2, No.1, Januari 2013
Publisher : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

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ABSTRACT In Vitro Study of the Effectivenes of Essential Oil Extracted from Lemongrass (Cymbopogon citratus) to Aspergillus Sp. Study on determining of the effectivenes of essential oil extracted from lemongrass (Cymbopogon citratus) in order to suppressing the growth of fruit rot fungi (Asoergillus sp.) and also to determining the minimum concentration that able to inhibit colony growth and spore formation of the Aspergillus sp. in vitro, was conducted in Laboratory of Plant Diseases from June into November 2012. The result showed that the fungi were isolated from rot fruit mango has similarities with the fungi Aspergillus sp. The characteristic of Aspergillus has cepted hyphae, growth appear colonies, conidiophores uncepted and stand up, the tip of conidiophore is swollen and produce vesicle form, the surface of vesicle is covered by fialid that usually simple and colored or colorless, fialid produced conidia which were form green, brown or black chain. Essential oil of lemongrass have an antifungal activity and it was found effective to inhibit growth of the fruit rot fungi Aspergillus sp. on PDA. The inhibition zone of the lemongrass essential oil against Aspergillus sp. were 85 mm which is include a very strong category. Effective concentration of essential oil of lemongrass to inhibit the growth of Aspergillus sp. are 0,6% - 1%. The persentase of inhibition in this concentration reached to 100%. On the other hand, the control treatment, 0,2%, and 0,4% concentration were 0%, 0%, and 28,53%, respectively. Minimum concentration which is capable to inhibit the formation of spores and growth of fungi colonies were 0,2% and 0,3% concentration. The compounds suspected as antifungal against Aspergillus sp. were ?-citral (geraniol) and ?-citral (neral). Keywords: Lemongrass (Cymbopogon citratus), essential oil, antifungi, Aspergillus sp.
Kultur In-vitro Anggur Laut (Caulerpa lentilifera) dan Identifikasi Jenis Mikroba yang Berasosiasi SUNITI, NI WAYAN; SUADA, I KETUT
Agrotrop: Journal on Agriculture Science Vol 2, No 1 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

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This research aims to find the best condition for the cultivation of fragment of Caulerpa lentilifera, to determine the factors that influence its growth in-vitro, and to identify the types of microbes associated. The study was conducted at the Marine Tech Laboratory and Marine Biology Laboratory of Udayana University. The study begins with the in-vitro culture of C. lentilifera seaweed at the incubator and the tank. The observations of the morphology and the seaweed growth to determine the suitable conditions, the influens of growth factors and microscopic observation to determine the types of microbes were associated. The best results in in-vitro culture in incubator was achieved by provision of 2 ml of PES in 500 ml of sea water, 5000 lux of light intensity, 12 hours irradiation and 100% medium replacement once a week. Best results in-vitro culture in the using net method was achieved by provision of 3500 lux light intensity, 12 hours irradiation and 70% culture replacement once a week. The microbes associated with the C. lentilifera came from the protozoa phylum, flagellate, and cilliata classes.
Pengendalian Penyakit Akar Gada yang Disebabkan oleh Plasmodiophora brassicae Wor. pada Tanaman Kubis (Brassica oleracea L. var. capitata L.) dengan Beberapa Ekstrak Tanaman HENDRIYANI, NI MADE YUNITA; SUADA, I KETUT; SUNITI, NI WAYAN
Agrotrop: Journal on Agriculture Science Vol 2, No 2 (2012)
Publisher : Agrotrop: Journal on Agriculture Science

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Various pathogens are able to obstruct the growth of cabbage. One pathogen that attacks thecabbage is Plasmodiophora brassicae Wor. which causes clubroot disease. Some efforts have appliedto control the disease included using synthetic fungicides, however that was not success, moreover itspolluting the environment as well. It is necessary to develop natural fungicide from plants extract thatcontain fungicidal compounds. The purpose of this study was to determine the effectiveness of theplants extract to control the clubroot disease of cabbage. The research design used was completelyrandomized design (CRD) with eight treatments and four replications. Gamal extract was the mosteffective plant to control the clubroot disease and promoted the plant growth as well. Gamal extractresulted the lowest number of club root, the lowest percentage of pathogen attacks, the highest plantdry weight, the lowest root dry weight, the greatest plant height, and the most number of leaves.
Identification of Endophytic Fungi from Rice Grain and It’s Inhibiting Ability by In Vitro Against Pyricularia oryzae Cav. SUNARIASIH, NI PUTU LINDA; SUADA, I KETUT; SUNITI, NI WAYAN
E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.2, April 2014
Publisher : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

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ABSTRACT Identification of Endophytic Fungi from Rice Grain and It’s Inhibiting Ability by In Vitro Against Pyricularia oryzae Cav. Fungal endophyte is a fungi that lives in the tissues of plants without causing disease symptoms on host plants. During symbiotic, many endophyte could produce antibiotic compounds, those can make endophyte microbes become biological control agents against pests and plant diseases. The aims of this study is to determine the types and the number of endophytic fungi spesies that exist in rice seeds stadia, also growth inhibitory ability against Pyricularia oryzae Cav. in vitro. Based on microscopic identification and DNA analysis were found 14 species of endophytic fungi. Endophytic fungi were able to inhibit the develop of Pyricularia oryzae Cav. amount. The greatest inhibitor was Unidentified-2 isolates of 65.6% followed by fungi Phaeosphaeriopsis musae, Sarocladium oryzae, and Sordariomycetes sp., with inhibition of 63.3%, 61.1%, and 58.9% respectively. Key words: endophytic fungi, inhibition, Pyricularia oryzae, rice,  In vitro.
Status Penyakit Layu pada Tanaman Cabai Rawit (Capsicum Frutescens L.) di Banjarangkan, Klungkung SUDARMA, I MADE; PUSPAWATI, NI MADE; SUNITI, NI WAYAN; BAGUS, I GUSTI NGURAH
Agrotrop: Journal on Agriculture Science Vol 4, No 2 (2014)
Publisher : Fakultas Pertanian Universitas Udayana

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Status of wilt disease in pepper (Capsicum frutescens L.) at Banjarangkan, Klungkung. Wilt disease in pepper , has led to a total yield loss in Banjarangkan, Klungkung regency. Until now the disease was still a threat to farmers in the area pepper. The purpose of research to study the disease includes symptoms, causes disease, the percentage of the disease and the rate of infection (r). Research using existing observations of the disease in three plots of local farmers. Each plot the observed number of diseased plants and entire plants. Samples of diseased plants put in a plastic bag, then placed in an ice box , to be observed macroscopically in the laboratory. The study was conducted in two places, namely surveys Banjarangkan disease in Klungkung , and isolation of the pathogen as well as pathogenicity test carried out in the Laboratory of Plant Pathology, Faculty of Agriculture, Udayana University, Jl. PB Sudirman Denpasar-Bali. The research was conducted from June to November 2013. The results showed that the pathogen that causes wilt disease in pepper at Banjarangkan, Klungkung regency was the fungus Fusarium oxysporum f.sp. capsici, with the disease incidence was 76.67%. Fusarium wilt disease epidemic in pepper indicated by the rate of infection, first gained 0.44 per unit per day, then decreases with time, 0.23, 0.12 and 0.11 per unit per day respectively. The rate of infection ranged from 0.11 to 0.44 per unit per day, this means that the moderate criteria.
Uji In-Vitro Daya Hambat Ekstrak Beberapa Jenis Tanaman terhadap Pertumbuhan Jamur Patogen Getah Kuning Buah Manggis SUADA, I KETUT; SUNITI, NI WAYAN; SUNARIASIH, NI PUTU LINDA
Agrotrop: Journal on Agriculture Science Vol 3, No 2 (2013)
Publisher : Agrotrop: Journal on Agriculture Science

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In-vitro Testing of Inhibiting Ability of Some Plants Extractto the Growth of MangosteenYellow Latex Pathogens. The yellow latex disease decreases fruit quality of mangosteen because ofthe exogenic latex causing dirty the fruit, and the endogenic latex makes the bitter fruit taste, thereforethe fruit does not deserve to be consumed. The causal agent of the disease are fungus i.e.Verticilliumalbo-atrum, Fusarium oxysporum,andPestalotia macrotricha, therefore the research was aimedto find the plant extract that could act as antifungal agent. Screening on many kinds of plant extractthrough well difusion method were found that crude water extract of 10% concentration of kayusugih, kayu tulak, sereh dapur, beluntas, puring, sirih hutan, temen, and dapdap were only effectiveto suppress Verticillium albo-atrum.Methanolic plant extract of 10% of endongplant was only effectiveagainst Fusarium oxysporum, while the extract that effective against Pestalotia macrotricha wasnot found yet.
Komparasi Laju Pertumbuhan Miselium Jamur Tiram Putih (Pleurotus ostreatus (Jacq. ex Fr) Kummer) pada Komposisi Media Bibit (F3) dan Baglog yang Berbeda SUDARMA, I MADE; WIJANA, GEDE; PUSPAWATI, NI MADE; SUNITI, NI WAYAN; BAGUS, I GUSTI NGURAH
Agrotrop: Journal on Agriculture Science Vol 3, No 2 (2013)
Publisher : Agrotrop: Journal on Agriculture Science

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The growth rate comparison of white oyster mushroom (Pleurotus ostreatus (Jacq. ex Fr)Kummer) mycelium in the composition of different seed (F3) and baglog media . Cultivation ofoyster mushroom (Pleurotus ostreatus (Jacq. ex Fr) Kummer) has grown rapidly along with the increasein income and health awareness. Oyster mushrooms growing need for media with a particular compositionin order to grow optimally. Oyster mushroom production is determined by the quality of the seeds (F3)is used, which is sourced from the media with good quality and composition. The research aimed todetermine the rate of growth of white oyster mushroom mycelium in the different composition of seedmedium (F3) (sawdust: fine bran: corn flour: CaCO3 ). The experiments was conducted at nurseriesand oyster mushroom development, Jl. Siulan Gang Zella No. 7 Denpasar, from June to August 2013.Each treatment contained 50 bottles, and 10 bottles only used as a sample, in environmental conditionswith temperature and humidity ranges, 20-29oC and 59-86% respectively . T-test was used todifferentiate the growth rate of white oyster mushroom mycelium with different compositions. Theresults showed that seeds (F3) derived from the growing media composition, sawdust (1 week old):fine bran: corn flour: CaCO3 (10:4:2:0,5) significantly different and better than the composition sawdust(age 1 month ): fine bran: corn flour (20:2:1:0.5), with a growth rate of mycelium in a mean 6.14±0.56cm/week and 1,81±0,82 cm/week, respectively. Spawn running in baglog with media composition10:4:2:0.5 was 2.77±1.22cm/week, but with composition media 20:2:1:0.5 mycelium could not grow.Effect of temperature and humidity on the growth rate of white oyster mushroom mycelium in seedmedia (F3) is not significantly.
Pemanfaatan Aeromonas hydrophila Untuk Mengendalikan Penyakit Layu Fusarium Pada Beberapa Varietas Sorgum Manis (Sorghum bicolor (L.) Moench ARIAWAN, I WAYAN GENTA; SUPRAPTA, DEWA NGURAH; SUNITI, NI WAYAN
E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.2, April 2015
Publisher : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

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The Use of Aeromonas hydrophila to Control Fusarium Wilt Disease on Several Varieties of Sweet Sorghum (Sorghum bicolor (L.) Moench) Objective of this study was to test the effectiveness of A. hydrophila to control wilt disease caused by Fusarium sp. on several varieties of sweet sorghum. Results of this study showed that application of rhizobacteria affected the growth and the yield of sweet sorghum. Under in vitro condition, treatments with A. hydrophila isolate KtBlt2 could suppress the growth of the Fusarium sp. with inhibitory activity 76.06% when compared to control. Under field condition treatment P6 (treatment wilt A. hydrophila on variety FS-501) could suppress Fusarium wilt disease with the lowest disease incidence by 3.24%. It is necessary to test the stability of A. hydrophila as biocontrol agent against Fusarium sp. Keywords : A. hydrophila, Fusarium sp., sweet sorghum