Dewi Fatma Suniarti
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430
Articles
3
Documents
EFFECT OF CHITOSAN ON PROTEIN CONTENT IN THE MEDIUM CULTURE OF OSTEOBLASTS EXPOSED TO OXIDATIVE STRESS

dentika Dental Journal Vol 16, No 1 (2011)
Publisher : Fakultas Kedokteran Gigi USU

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Abstract

Abstract Chitosan is a derivative of chitin which has potential for use in bone regeneration and has been reported can stimulate bone formation. Oxidative stress as one cause of bone damage, was found increased in osteoporosis, periodontitis and arthritis. One of the species oxygen reactive (ROS), hydrogen peroxide, has been reported can inhibit osteoblast pro-liferation. This study was aimed to investigate the effect of various chitosan concentrations on protein content in the culture medium of human osteoblast-like cell line, MG 63, which was exposed to hydrogen peroxide. MG 63 cells were exposed to various chitosan concentrations (% w/v) 0.1, 0.2, 0.4, and 1.6%.  Culture cells without chitosan were used as a control. Cells were growth with α-MEM medium (37ºC, 5% CO2) until they became confluent, then they were exposed to hydrogen peroxide for 4 hours. The protein content in the culture medium was measured by using Bradford protein assay at 655 nm wavelength. The result showed that hydrogen peroxide decreased protein concentration in the medium culture compared with group without hydrogen peroxide. Treatment group with chitosan concentration 0.4% and 1.6% exhibited a significant increasing of protein concentration in osteoblast culture medium compared with control. In conclusion, in osteoblast culture medium chitosan can inhibit the decreasing of total protein concentration which was caused by oxidative stress. Key words: osteoblast, hydrogen peroxide, chitosan, protein concentration

EFEK SAMPING OBAT YANG BERMANIFESTASI DALAM RONGGA MULUT

Journal of Dentistry Indonesia Vol 10, No 2 (2003): AUGUST
Publisher : Faculty of Dentistry, University of Indonesia

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Abstract

Every drug can produce side effect, even when used according to standard or recommended methods of administration.Side effect of drugs can involve every organ and system of the body and are frequently mistaken for signs of underlying disease. Similarly, the mouth and associated structures can be affected by many drugs or chemicals. Good oral health including salivary function is very important in maintaining whole body health.Certain drugs can cause change in the oral cavity. Intrinsic discoloration can occur with the tertracyclines and extrisic stains are associated with certain metal containing liquids. Ginigival hyperplasia can occur with some anticonvulstants, calcium channel blockers, and cyclosporine. Xerostomia are produced by hypertensives, antipsikosis, antihistamin, antikolinergik and many other drugs. Regarding different parts of the oral system, drug side effects can be categorized to oral mucosa and tongue, periodontal tissue, dental structures, salivary glands, cleft lip and palate, muscular and neurological disorders, taste disturbances and drug-induced oral infection.The knowledge about drug-induced oral side effects helps dentist and to better diagnose oral disease, administer drugs, improve patient compliance during drug therapy, and may influence a more rational use of drugs.

RAMBUTAN HONEY PHARMACEUTICAL STANDARDIZATION FOR WOUND HEALING TREATMENT

Bionatura Vol 17, No 1 (2018): Bionatura Maret 2018
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan

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Abstract

Human wounds require proper management so they may heal faster and without complication. Honey is empirically effective to accelerate wound healing but scientifically has been proven yet as a pharmaceutical product is very limited. This study aimed to obtain rambutan honey pharmaceutical grade by setting specific and nonspecific parameters of simplicia. The research method was experiments laboratory by nonspecific parameters test (diastase enzyme activity, HMF, pH value, moisture content, ash content, glucose level, heavy metal contamination: Pb, Cd, Hg, and As, chloramphenicol, microbial contamination: coliform, and yeasts) as well as specific parameters (simplicia identity and organoleptic test). The results showed that rambutan honey proved had good quality of specific and nonspecific parameters. Rambutan honey water content has been pharmaceutical standardized according to drug standard that is to be 10.15%. Based on the results of this study it is necessary to test rambutan honey efficacy in vivo and in vitro and effects of rambutan honey topical products to humans towards phytopharmaca medicine.