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Penggunaan Batang Tanaman Betadin (Jatropha mulitifida Linn) untuk Meningkatkan Jumlah Trombosit pada Mus musculus

MEDIA MEDIKA INDONESIANA 2011:MMI Volume 45 Issue 2 Year 2011
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

ABSTRACTThe use of plant stem betadin (Jatropha mulitifida Linn) to increase the number of thrombocyt in Mus musculusBackground: Jatropha multifida Linn has been used by the people of Bengkulu as an ornamental plant, some of people use as medicinal for a new wound. That is why known as betadin plants. Jatropha multifida Linn contain flavonoids compound. The aims of this study is to isolate the total flavonoids compound from stems of Jatropha multifida Linn, and then it was used to increase the number of thrombocyt.Methods: Stem Jatropha multifida Linn was isolated by EtOH (96%), the filtrate was evaporated using a rotary evaporator. Fifteen of healthy Mus musculus with 20-50 g, were grouped into 4; group 1 (P0) was treated by aquadest, group 2 (P1) was treated with 0.2 mL of palm oil, group 3 (P2) was treated with a dose of guava extract (0.028 g/kgBW), group 4 (P3) and (P4) were given total flavonoids of Jatropha multifida Linn stems in which each was given with a dose of 0.028g/kgBW and 0,056g/kgBW. After 24 hours of treatment, the thrombocyt changes owere counted under a microscope.Results: The rendement of flavonoids compound in the stem of Jatropha multifida Linn which can be isolated by EtOH is 4%. The flavonoids compound from Jatropha multifida Linn stems at a doses of 0.028 g/kgBW could increased the number of thrombocyt to 543.000/mm3 and at a 0.56 g/kgBW to 813.000/mm3.Conclusion: The flavonoids compoud from Jatropha multifida Linn stems at a doses of 0.028 g/kgBW and doses of 0.56 g/kgBWcould increased the number of thrombocyt at Mus musculus.Keywords: Jatropha multifida Linn, trombocyt, flavonoids, Mus musculusABSTRAKLatar belakang: Jatropha multifida Linn oleh masyarakat Bengkulu digunakan sebagai tanaman hias, sebagian masyarakat ada yang menggunakan sebagai tanaman untuk obat luka baru. Itulah sebabnya maka dikenal dengan tanaman betadin. Batang tanaman Jatropha multifida Linn mengandung senyawa flavonoid. Penelitian ini bertujuan untuk mengisolasi senyawa flavonoid total dari batang tanaman Jatropha multifida Linn, kemudian diujicobakan untuk melihat peningkatan jumlah trombosit pada Mus musculus.Metode: Batang tanaman Jatropha multifida Linn diisolasi dengan maserasi menggunakan EtOH (96%), filtrat diuapkanmenggunakan rotary evaporator. Lima belas Mus musculus sehat dengan berat 20-50 g, dikelompokkan menjadi 4 yaitu kelompok 1 (P0) diberi perlakuan secara oral dengan aquades, kelompok 2 (P1) diberi perlakuan secara oral dengan 0,2 mL minyak manis, kelompok 3 (P2) diberi perlakuan secara oral dengan ekstrak daun jambu biji dengan dosis 0,028 g/kgBB, kelompok 4 (P3) dan kelompok 5 (P4) diberi perlakuan secara oral dengan senyawa flavonoid total batang tanaman Jatropha multifida Linn masingmasingdengan dosis 0,028 g/kgBB dan 0,056 g/kgBB. Setelah 24 jam untuk setiap perlakuan, kenaikan jumlah trombosit dihitung dibawah mikroskop.Hasil: Rendemen flavonoid total dari batang tanaman Jatropha multifida Linn yang dapat diisolasi menggunakan EtOH sebesar 4%. Pemberian senyawa flavonoid total dari batang Jatropha multifida Linn secara oral pada Mus musculus dengan dosis 0,028 g/kgBB mampu meningkatkan jumlah trombosit sebesar 543.000/mm3 dan dosis 0,056 g/kgBB mampu meningkatkan jumlah trombosit sebesar 813.000/mm3.Simpulan: Pemberian senyawa flavonoid total dari batang tanaman Jatropha multifida Linn dengan dosis 0,028 g/kgBB dan 0,056 g/kgBB secara oral mampu meningkatkan jumlah trombosit pada Mus musculus, dan semakin tinggi dosis yang diberikan semakin tinggi kenaikan jumlah trombosit.

Development of The Stem of Jatropha multifida Linn as A New Antimalarial through Erythrocytes Test on Mus musculus Infected by Plasmodium berghei

Journal of Biomedicine and Translational Research Vol 1, No 1 (2015): April 2015
Publisher : Faculty of Medicine, Diponegoro University

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Introduction : Jatropha multifida Linne is known as Betadin plant by the Bengkulunese. The stem extract of J. multifida has antibacterial activity. This study examined the potential of stem extract of J. multifida to be developed as an anti-malarial drugs through trials in M. musculus whose erythrocytes infected by Plasmodium berghei.Methods : Phytochemical test of the stem of J. multifida to test the flavonoids, alkaloids, tannins, saponins, terpenoids and steroids. The stem extract of J. multifida obtained by maceration with 96% ethanol. 30 M. musculus divided 6 groups, each consist of 5 animals. P0 is the negative control groups that were not infected by P berghei. P1 is the positive control groups that were only infected by P berghei, P2 is a comparison groups that were infected with P berghei and chloroquine, groups P3, P4, and P5 were infected by P berghei and treated with stem extract of J multifida with consecutive doses 0.028 g / kgbw, 0.056 g / kgbw and 0.084 g / kgbw. After 24 hours, the number of erythrocytes was observed with hemocytometer then was counted the number of uninfected erythrocytes by P berghei using a microscope at 1000x magnification.Results :The stem extract of J. multifida at doses of 0.028 g/kgbw, 0.056 g/kgbw and a dose of 0.084 g/kgbw were able to increase the number of uninfected erythrocytes by P berghei in M. musculus, respectively an average of 9.135million cell eritrosit/mm3, 7.618 millioncell eritrosit/mm3, and 9.856 millioncell eritrosit/mm3. The ability of stem extract of J. multifida in increasing the number of erythrocytes uninfected with P berghei was much higher than the malaria drug chloroquine diposphat. On one way ANOVA analysis of Fcount ( 13,2 ) > Ftable (2.76 ) , with (α = 0.05), there are noticeable differences in the provision of treatment. The increasing number of uninfected erythrocytes by P berghei was due to the content of flavanol glycosides in J multifida stem. Flavanol glycosides was expected to form Flvanolglycosides-heme complex that could inhibit the formation of P berghei parasites HemazoinConclusion:The stem extract of J. multifida could be developed as a potential anti-malarial drugs since it could increase the number of uninfected erythrocytes by P berghei in M. musculus. Its ability was much higher than the malaria drug chloroquine diposphat

UJI AKTIVITAS ANTIPLASMODIUM FRAKSI N-HEKSANA DAUN Peronema canescens TERHADAP Mus musculus

Alotrop Vol 1, No 1 (2017)
Publisher : FKIP University of Bengkulu

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Abstract

Peronema canescens (Sungkai) has been used in Bengkulu as raw herbal remedy to reduce the fever, some people are using as a malaria drug. P.  canescens leaves contain alkaloids, flavonoids, tannins and terpenoids – steroids .This study aims to analyze the effect of P.  canescens leaves  n-hexane fraction.  against paracetemias in Mus musculus infected with Plasmodium berghei, then to prove whether the . P.  canescens leaves n-hexane fraction  has potential as alternative medicine for malaria .   To make P. canescens extract , leaves was macerated using EtOH (96%), then filtrate was evaporated using a rotary evaporator, then fractionated with n-hexane. The 25 healthy  M.musculus  weighing 20-40g each, infected with P.berghei, grouped into 5 ie group 1 (K-) was treated orally with aquades, group 2 (K +) was treated orally with 0.42 mg of Chloroquine, Group 3 (P1) Group 4 (P2), group 5 (P3) was treated orally with a P.  canescens leaves n-hexane fraction  each at a dose of 0.028 ,  0.056 and 0.084 g / kgBW. After 3 days of digestion for each treatment, the amount of erythrocytes was calculated under a microscope. : The giving of the with dose 0,028 g / kgBW able to inhibit paracetemia 33,49%,  0,056 g / kgBW able to  57.91% and  0,084 g / kgBW able to  61.69%   The conclusion is the P. canescens leaves n-hexane fraction  at a dose of 0.028 , 0.056 , and 0.084 g / kgBW orally wil be decreased the amount of paracetemia in M.musculus, and the higher the dose given the higher percent of the inhibition.

AKTIVITAS FRAKSI ETANOL DARI EKSTRAK DAUN Peronema canescens TERHADAP TINGKAT PERTUMBUHAN Plasmodium berghei

Alotrop Vol 2, No 1 (2018)
Publisher : FKIP University of Bengkulu

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 [THE ACTIVITY OF THE Peronema canescens LEAVES ETHANOL EXTRACTS FRACTION AGAINST Plasmodium berghei GROWTH RATE ] The purpose of this research was to identify the secondary metabolites of compounds contained in the fraction of ethanol from leaves of Peronema canescens (Sungkai) as well as to measure the level of activity of ethanol fraction antiplasmodium leaf P.canescens against the male mice (Mus musculus) infected with Plasmodium berghei which is parasitic hemaprotozoa that can cause malaria in rodents such as rats and mice as well as molecular similarity with the parasite Plasmodium. falciparum that causes malaria in humans. The methods used in this study i.e. in maceration extraction methods to attract secondary metabolite compounds from the leaves of P.canescens followed by oil bath method to get a fraction of ethanol with the purpose of separating polar compounds from a mixture of secondary metabolite compounds contained in the sample leaf P.canescens. Assay activity against antiplasmodium males mice infected with P. berghei is done by dividing into the 5 mice group treatment i.e. Group K (-) that are quads, group K (+) given kloroquin anti malarial drug, and P1, P2, P3, which each group was given a dose of ethanol fraction from leaves of P. canescens with each dose of 0.028 (P1); 0.056 (P2), and 0.0084 g/kgBB (P3).The results of research conducted showed that administering ethanol fraction from leaves of P. canescens antimalarial activity can increase with very real on the best dose of kg BB 0084 g i.e. with the percentage inhibition of 54.06%.

Pembelajaran probing prompting untuk meningkatkan berpikir kritis siswa anggota kelompok ilmiah remaja

PendIPA Journal of Science Education Vol 2, No 2 (2018): june
Publisher : PendIPA Journal of Science Education

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Abstrak Kegiatan ilmiah mahasiswa dapat dilakukan melalui kegiatan ekstrakurikuler Kelompok Ilmiah Remaja (KIR). Anggota KIR diharuskan memiliki keterampilan berpikir kritis dalam mencari topik untuk menulis makalah ilmiah. Salah satu metode pembelajaran yang dapat dilakukan untuk meningkatkan pemikiran kritis siswa KIR adalah dengan mempelajari instruksi yang dibantu oleh video-assisted. Sebuah eksperimen semu dengan desain desain one pretest-posttest dilakukan untuk mengukur tingkat berpikir kritis anggota KIR dengan memberikan pretest sebelum mempelajari probing yang mendorong tes video-aided dan post setelah belajar dorongan video-assisted probing. Analisis data dilakukan dengan menggunakan uji efektivitas dan uji Wilcoxon. Hasil data dengan efek pengukuran nilai efek diperoleh E> 0,8 yang berarti ada peningkatan berpikir kritis sebelum dan sesudah belajar. Uji Wilcoxon diperoleh nilai p (Z) = 0,0344. Nilai p (Z) <0,05 yang berarti kemampuan berpikir kritis siswa meningkat dari pretest ke posttest. Kata kunci: Probing prompting; berpikir kritis; kelompok ilmiah remaja

Pembelajaran Fisika Konsep Kalor Dengan Menggunakan Media Pirolisis Sampah Plastik Untuk Meningkatkan Ketrampilan Berpikir Kritis Siswa Di SMAN 3 Bengkulu Tengah

PendIPA Journal of Science Education Vol 1, No 1 (2017): october
Publisher : University of Bengkulu

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The objectives of this research are (1) to know the value of density, viscosity, fog point, oil absorption caloric, odor, color and fire sensitivity, in pyrolysis of plastic garbage from condenser one and two adsorbed using zeolite and activated charcoal, (2) the content contained in pyrolysis plastic waste oil adsorbed using active zeolite and charcoal, (3) knowing differences in critical thinking skills among students who learn to use guided inquiry learning model with students who study conventionally on physics learning. Convert plastic waste into fuel oil using a pyrolysis process with two water condensers, then plastic waste pyrolysis oil adsorbed using activated zeolite and charcoal. The results showed that the density value of the adsorbed oil for one condenser was 772 Kg / m3 and the condenser was 770 Kg / m3, the viscosity value of the adsorbed oil from the condenser one and two had the same value of 0.49 m2 / s, the fog point value oil that has been adsorbed on the condenser yield one and two is the same value that is -18oC, the calorific value of oil absorption, color, odor, and fire sensitivity shows the more adsorbent and the longer the adsorption process, the oil absorbs the smaller, , the odor does not sting, and the fire sensitivity gets smaller. GC-MS test results obtained the most compounds contained in pyrolysis oil plastic waste that has been adsorbed that is 1,3,5-trimethylcyclohexene. The implementation of physics learning on the concept of heat shows that there are differences in critical thinking skills in control and experiment class. Based on different test on posttest result the first material got 0,022 and postest second material equal to 0,047, the value is smaller than level of significance 0,05 so Ha accepted.

Analisis kadar komponen kimia pelepah sawit varietas dura sebagai bahan baku pulp yang diterapkan pada pembelajaran kimia

PendIPA Journal of Science Education Vol 2, No 1 (2018): February
Publisher : University of Bengkulu

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ABSTRAK Penelitian ini bertujuan untuk menentukan kadar komponen kimia yang meliputi zat ekstraktif, lignin, holoselulosa dan ?-selulosa pelepah sawit varietas dura, menjelaskan pengaruh bagian pelepah sawit varietas dura terhadap kadar ekstraktif, lignin, holoselulosa dan ?-selulosa, membandingkan perbedaan kadar komponen kimia antar bagian pangkal, tengah dan ujung dari pelepah sawit varietas dura, mengindentifikasi gugus fungsi yang ada pada sampel (lignin, holoselulosa dan ?-selulosa) dengan menggunakan metode spektroskopi FTIR, membandingkan pengaruh perbedaan hasil belajar sebelum dan sesudah diberi pengembangan konsep makromolekul dengan melihat video yang diaplikasikan pada Lembar Kerja Peserta Didik (LKPD) terhadap cara berfikir kritis siswa Olimpiade Kimia. Zat ekstraktif, holoselulosa, ?- selulosa dan lignin dianalisis dengan menggunakan Tappi Test methode :204, T 203, T:222 serta diidentifikasi dengan spektroskopi FTIR. Hasil penelitian Kadar komponen kimia pada pelepah sawit varietas dura menurut bagiannya dari pangkal, tengah, ujung berturut-turut dengan rerata untuk kadar zat ekstraktif 8,92%, 7,80% , 7,23%. Lignin 24,89 %, 26,87 %, 24,17 %. Holoselulosa 84,11 %, 83,47 %, 82,24% dan ?- selulosa 45,76 %, 45,76 %, 45,54%. Penelitian pendidikan dengan desain one-group pretes-postes. Penelitian pendidikan dilakukan dengan melakukan proses pembelajaran menggunakan Video dan LKPD sesuai dengan keterampilan berpikir kritis. Pada implementasi dalam pembelajaran terdapat perbedaan pada hasil belajar kimia sebelum dan sesudah proses pembelajaran menggunakan video dan LKPD. Rata-rata nilai pretest 42,25 sedangkan rata-rata postest sebesar 83,50 dan terdapat peningkatan hasil belajar setelah dilakukan proses pembelajaran menggunakan Video dan LKPD secara signifikan sebesar 41.25. Kata kunci: zat ekstraktif; holoselulosa; selulosa; lignin; LKPD.

Uji Aktivitas Ekstrak Biji Caesalpinia Sp terhadap Jumlah Eritrosit Mus musculus yang Diinfeksi Plasmodium berghei serta Implementasinya dalam Pembelajaran Kimia

PendIPA Journal of Science Education Vol 2, No 3 (2018): October
Publisher : FKIP University of Bengkulu

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Abstract

AbstraCT[Activity test of Caesalpinia sp seed extract on erythrocytes amount of Mus musculus infected by Plasmodium berghei and its implementation in chemistry learning]. The aims of this study were to (1) determine the activity of seed extract (Caesalpinia sp) on the number of Erythrocytes of M. Musculus of Swiss Webster Strain infected by P.berghei, (2) Find  the active compounds obtained from the isolation of Caesalpinia sp seed extract (3) Find the differences in student learning outcomes about the separation of secondary metabolites using thin layer chromatography (TLC) which is taught in chemistry learning by using LKS. In this study Caesalpinia sp seed extract was purified by thin layer chromatography and column coromatography, and was identified by IR and 1H-NMR spectroscopy. M. musculus infected with P. berghei was given Caesalpinia sp seed extract at a dose of 0.028 g/Kgbb, 0.056 g/Kgbb, and 0.084 g / Kgbb by gavage, while the positive control group was given Kloroquin diphosphate, negative control was given olive oil. Caesalpinia sp seed extract with a dose of 0.056 g/Kgbb inhibited the highest plasmodium, which was 42.89% and even exceeded the malaria drug commonly used chloroquin diphosphate 29.85%. Caesalpinia sp seed extract capable of reducing plasmodium in erythrocytes is also able to increase the number of uninfected erythrocytes. The active compounds obtained from the isolation of Caesalpinia sp seed extract are alkaloids, flavonoids, saponins, and terpenoids. In the implementation of extracurricular group learning is taught by using worksheets. There are significant differences where the average posttest score is 88.67 and the average pretest is 32.1. Keywords: Caesalpinia Sp.; Erythrocyte; Plasmodium berghei; Mus muscullus; Student working sheet.

Isolasi ekstrak kulit batang J.multifida L., serta implementasinya pada modul pembelajaran Kimia Organik Bahan Alam

PendIPA Journal of Science Education Vol 2, No 2 (2018): june
Publisher : PendIPA Journal of Science Education

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 Abstrak Penelitian ini bertujuan untuk mengetahui bagaimana hasil karakterisasi senyawa metabolit sekunder dari hasil isolasi kulit batang J. multifida L dan bagaimana peningkatan hasil belajar mahasiswa pada pembelajaran kimia organik bahan alam menggunakan modul. Kulit batang J. multifida L dimaserasi menggunakan etanol (96%),filtrat diuapkan menggunakan rotary evaporator, kemudian difraksinasi cair-cair menggunakan pelarut dengan kepolaran bertingkat berturut-turut dengan pelarut n-heksana dan etil asetat. Tahap selanjutnya, Isolasi sampel menggunakan kromatografi kolom. Implementasi penggunaan modul dilakukan pada mahasiswa Pendidikan kimia semester VI pada mata kuliah kimia organik bahan alam (KOBA). Mata kuliah ini hanya diikuti oleh satu kelas yang bertindak sebagai kelas eksperiment. Pada awal pembelajaran dilakukan pretest dan diakhir pembelajaran diberikan posttest. Senyawa metabolit sekunder yang dapat diisolasi dari fraksi n-heksan pada kulit batang J.multifida L., Berdasarkan hasil analisis spektroskopi diduga bahwa senyawa memiliki kerangka senyawa steroid dengan golongan Stigmasterol dengan rumus molekul C29H48O. Pembelajaran Kimia Organik Bahan Alam (KOBA) dengan menggunakan modul memberikan peningkatan hasil belajar mahasiswa, terdapat perbedaan secara signifikan antara nilai rata-rata posttest (82,4) dengan rata-rata nilai pretest (58,4) dan KKM (75). Kata kunci: Kulit batang J.multifida L; isolasi; hasil belajar; modul.

Pengembangan media pembelajaran kimia menggunakan video untuk mengukur kemampuan berfikir kritis siswa

PendIPA Journal of Science Education Vol 2, No 2 (2018): june
Publisher : PendIPA Journal of Science Education

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ABSTRAKPenelitian ini bertujuan untuk mengembangkan media pembelajaran dalam bentuk video pembelajaran kimia dan digunakan untuk mengukur kemampuan berpikir kritis siswa. Metode yang digunakan adalah penelitian dan pengembangan (Research and Development) pembelajaran kimia pada bahan minyak bumi dengan model Dick dan Carey, melalui tahapan: desain, validasi, revisi dan tes formatif untuk siswa kelas XI di SMAN 1 Bengkulu Tengah. Kemampuan berpikir kognitif siswa dapat diukur dan dilihat dari hasil tes formatif, data yang diperoleh dalam bentuk data kuantitatif dan dilakukan analisis deskriptif untuk menilai kemampuan berpikir kritis siswa. Berdasarkan hasil tes formatif, 13,3% siswa kurang kritis, 60% siswa kritis, 20% siswa kritis dan 6,67% sangat penting.Kata kunci: Pengembangan; media; pembelajaran kimia; video; berfikir kritis.