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Yeast Isolation for Bioethanol Production RURIANI, EKA; SUNARTI, TITI CANDRA; MERYANDINI, ANJA
HAYATI Journal of Biosciences Vol 19, No 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.19.3.145

Abstract

We have isolated 12 yeast isolates from five different rotten fruits by using a yeast glucose chloramphenicol agar (YGCA) medium supplemented with tetracycline. From pre-screening assay, four isolates exhibited higher substrate (glucose-xylose) consumption efficiency in the reaction tube fermentation compared to Saccharomyces cerevisiae dan Saccharomyces ellipsoids as the reference strains. Based on the fermentation process in gooseneck flasks, we observed that two isolates (K and SB) showed high fermentation efficiency both in sole glucose and mixed glucose-xylose substrate. Moreover, isolates K and SB produced relatively identical level of ethanol concentration compared to the reference strains. Isolates H and MP could only produce high levels of ethanol in glucose fermentation, while only half of that amount of ethanol was detected in glucose-xylose fermentation. Isolate K and SB were identified as Pichia kudriavzeevii (100%) based on large sub unit (LSU) ribosomal DNA D1/D2 region.
SIFAT FUNGSIONAL PATI GARUT HASIL MODIFIKASI HIDROKSIPROPILASI DAN TAUT SILANG [Functional Properties of Hydroxypropylated and Crosslinked Arrowroot Starch] Maulani, Rijanti Rahaju; Fardiaz, Dedi; Kusnandar, Feri; Sunarti, Titi Candra
Jurnal Teknologi Dan Industri Pangan Vol 24, No 1 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/6958

Abstract

SIFAT FUNGSIONAL PATI GARUT HASIL MODIFIKASI HIDROKSIPROPILASI DAN TAUT SILANG [Functional Properties of Hydroxypropylated and Crosslinked Arrowroot Starch] Rijanti Rahaju Maulani1)*, Dedi Fardiaz2), Feri Kusnandar2) dan Titi Candra Sunarti3) 1) Program Studi Rekayasa Pertanian, Sekolah Ilmu dan Teknologi Hayati, Institut Teknologi Bandung,  Jl. Ganesa No. 10, Bandung, Indonesia 2) Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor,  Kampus IPB Darmaga, Bogor, Indonesia 3) Departemen Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor, Indonesia   Diterima 25 September 2012 / Disetujui 29 Juli 2013 ABSTRACT   Dual-modified arrowroot starch using hydroxypropylation and cross-linking methods was carried out to overcome the deficiency in of native arrowroot starches for food processing application. The modification applied the combination concentration of propylene oxide (8, 10, and 12%) and ratio of sodium trimetaphosphate (STMP):sodium tripolyphosphate (STPP) (1%:4%, 2%:5%, and 3%:6%). The resulting dual-modified arrowroot starches had lower gelatinization temperature (68.45–70.00ºC) than that of native arrowroot starch (72.85ºC). The modified arrowroot starches also had a higher peak viscosity (>5500 cp) than that of native arrowroot starch (4209 cP). Breakdown and setback viscosity of modified arrowroot starch was higher values than the native. At acidic pH, the viscosity decreased at different levels of temperature changes as compared to that of normal pH. Modified starch made with 8% propylene oxide and ratio of STMP: STPP 2%:5% and 3%:6%; as well as that made with 10% propylene oxide and 1% STMP:4% STPP had the lowest syneresis tendency. Decrease in the paste clarity occured with increasing concentration of STMP:STPP. The sedimentation volume of the modified starch was higher (29.17-35.83%) than  that of native starch (28.08%), except for those made with 1% STMP: 4% STPP at concentration of propylene oxide 8% and 12%. The gel strength increased (61.77-78.97 gf) at 8% propylene oxide, but decreased (66.50-47.77 gf) at higher concentrations.
PERUBAHAN STRUKTUR PATI GARUT (Maranta arundinaceae) SEBAGAI AKIBAT MODIFIKASI HIDROLISIS ASAM, PEMOTONGAN TITIK PERCABANGAN DAN SIKLUS PEMANASAN-PENDINGINAN [Structure Changes of Arrowroot (Maranta arundinaceae) Starch as Influenced b Faridah, Didah Nur; Fardiaz, Dedi; Andarwulan, Nuri; Sunarti, Titi Candra
Jurnal Teknologi Dan Industri Pangan Vol 21, No 2 (2010): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/3410

Abstract

PERUBAHAN STRUKTUR PATI GARUT (Maranta arundinaceae)  SEBAGAI AKIBAT MODIFIKASI HIDROLISIS ASAM, PEMOTONGAN TITIK PERCABANGAN DAN                    SIKLUS PEMANASAN-PENDINGINAN [Structure Changes of Arrowroot (Maranta arundinaceae) Starch as Influenced by Acid Hydrolysis, Debranching and Autoclaving-Cooling Cycle Modifications] Didah Nur Faridah1)*, Dedi Fardiaz1), Nuri Andarwulan1) dan Titi Candra Sunarti2) 1) Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor. 2) Departemen Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Institut Pertanian Bogor. Diterima 05 Agustus 2010 / Disetujui  20 Desember 2010 ABSTRACT   The effects of lintnerization (2.2 N HCl, 2 hours), debranching with pullulanase (1.3 U/g and 10.4 U/g starch) and/or three-auctoclaving-cooling cycles at 121oC for 15 minutes on the changes of arrowroot starch structures were studied. The structural modifications of amylose and amylopectin were measured by Gel Permiation Chromatography (GPC), and the distribution of degree of polimerization (DP) was analyzed by Fluorophore-Assisted Capillary Electrophoresis (FACE). The GPC profile of native starch using Toyopearl HW-65S gel gave mainly two fractions. Fraction I (Fr. I) was a high molecular weight component and Fraction II (Fr. II) was a low molecular weight component. After acid modification, the carbohydrate content of Fr. II increased while that of Fr. I decreased. The amount of  DP of 6 to 8 increased in all modified arrowroot starches. The GPC and FACE analyses showed that all starch modification techniques caused the structural changes of amylopectin molecules to form short chain amyloses.   Key words: arrowroot starch, degree of polimerization, FACE (Fluorophore-Assisted Capillary Electrophoresis) and GPC (Gel Permiation Chromatography)
Immobilization of Extracellular Xylanase from Streptomyces sp. 45 I-3 for Hydrolysis of Corncob Xylan Sunarti, Titi Candra; Mutia, Ferry; Gusmawati, Niken Financia; Lestari, Yulin; Meryandini, Anja
Jurnal Teknologi Dan Industri Pangan Vol 20, No 1 (2009): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/313

Abstract

PENGGUNAAN  XILANASE  Streptomyces sp. 45 I-3 AMOBIL UNTUK HIDROLISIS XILAN TONGKOL JAGUNG [Immobilization of Extracellular Xylanase from Streptomyces sp. 45 I-3 for Hydrolysis of Corncob Xylan ] Anja Meryandini1),2), Titi Candra Sunarti3), Ferry Mutia3), Niken Financia Gusmawati4), dan Yulin Lestari2) 1)Pusat Penelitian Sumberdaya Hayati dan Bioteknologi IPB,Gedung PAU, Kampus IPB Darmaga 16680 2)Departemen Biologi, FMIPA-IPB, Gedung Fapet Lt 5 Wing 1, Kampus IPB Darmaga 16680 3)Departemen Teknologi Industri Pertanian, FATETA-IPB, Kampus IPB Darmaga 16680 4)PS Bioteknologi-Sekolah Pasca Sarjana IPB,  Gedung PAU, Kampus IPB Darmaga 16680 Autor korespondensi: ameryandini@yahoo.com Diterima 15 Desember 2008/Disetujui 13 Juni 2009 ABSTRACT   Xylan extraction from corncob is done by using alkaline as solvent. Xylan extraction from corncob could give the yields as 10.9%. One percent of corncob xylan is used as substrate to produce the xylanase, compared to oatspelt xylan. Immobilization of xylanase was performed using 1% EudragitTM S100 solution (w/v), with 5:1 volume ratio of xylanase and 1 % EudragitTM S100 (w/v). Activity of the immobilized xylanase was decreased to 23.97% compared with free xylanase. Immobilized xylanase have optimum pH and temperature at 6.0 and 40°C  respectively, have also thermal stability at 30–40°C for an hour. Immobilized xylanase could be reused, but its activity decreased to 52.38% after 3 times application.   Key words : xilanase, Streptomyces, amobile
Adsorpsi Surfaktan Nonionik Alkil Poliglikosida pada Antar muka Fluida-Fluida Adisalamun, Adisalamun; Mangunwidjaja, Djumali; Suryani, Ani; Sunarti, Titi Candra; Arkeman, Yandra
Jurnal Rekayasa Kimia & Lingkungan Vol 9, No 1 (2012): Jurnal Rekayasa Kimia & Lingkungan
Publisher : Chemical Engineering Department, Syiah Kuala University, Banda Aceh, Indonesia

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Abstract

Nonionic surfactants have been used extensively in various industrial applications such as cleaning, personal care, crop protection, paint and coating, textile finishing, emulsion stabilization, food and leather processing. In this study, we have studied the adsorption of three nonionic surfactants of alkyl polyglycoside, namely commercial APG from Cognis and APG produced from glucose as well as APG synthesized from sago starch, at fluid–fluid interfaces. The variation of surface and interfacial tension with the concentration of surfactant in the bulk was studied, and the data were fitted using a surface equation of state derived from the Langmuir isotherm. The agreement between ?(c) data and Langmuir isotherm model was very good.Keywords: adsorption, air/water interface, interfacial tension, surface tension
PENINGKATAN KECERAHAN PADA PROSES SINTESIS SURFAKTAN NONIONIK ALKIL POLIGLIKOSIDA (APG) BERBASIS TAPIOKA DAN DODEKANOL Bastian, Februadi; Suryani, Ani; Sunarti, Titi Candra
REAKTOR Volume 14, Nomor 2, Oktober 2012
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/reaktor.14.2.143-150

Abstract

Alkylpolyglycosides (APG) is a nonionic surfactant that has been getting some green labels such asEcocert, EU Eco-flower and Green Seal as an environmentally friendly surfactant. Sugar is the mainraw material which is supplied the hydrophilic group, and fatty alcohol as hydrophobic group. Someundesirable compounds formed during the APG production and caused low quality. The aim of thisresearch to increase the quality and performance of APG, by controlling its process. Addition 0-10%of activated carbon and 0-0.3% of NaBH4 in APG pre-purification process; 2% (w/w) of H2O2, 35%and 500 ppm of MgO in the bleaching process were examined to process high quality and highperformance of APG. The best APG was obtained from purification step by addition 0% of activatedcarbon and 0.2% of NaBH4, with the characteristics of clarity of 59.02(%T); the ability to reducesurface and interfacial tensions at 1% concentration were 61.94% and 95.6% respectively; 81.71%of stability of emulsion, 62.5% of foam height and stable up to 315 minutes. Alkil Poliglikosida (APG) merupakan surfaktan nonionic yang telah mendapatkan beberapa greenlabel seperti Ecocert, EU Eco-flower Green Seal dan sebagainya sebagai surfaktan yang ramahlingkungan. Bahan dasar APG yaitu gula untuk membentuk gugus hidrofiliknya dan fatty alcoholuntuk membentuk gugus hidrofobiknya. Permasalahan dalam produksi APG adalah timbulnya warnagelap yang tidak dikehendaki yang menyebabkan penurunan mutu APG. Tujuan dari penelitian iniyaitu untuk meningkatkan mutu dan kinerja dari APG. Untuk meningkatkan kecerahan APG, makasebelum dilakukan proses destilasi dilakukan penambahan arang aktif 0-10% serta NaBH4 0-0,3%.Pada proses pemucatan ditambahkan 2% (b/b) H2O2 35% dan MgO 500 ppm. Hasil terbaik yaitupenambahan arang aktif 0% dan NaBH4 0,2% dengan karakteristik kecerahan 59,02(%T);kemampuan menurunkan tegangan permukaan dan antar muka pada konsentrasi 1% sebesar 61,94%dan 95,6%; kestabilan emulsi 81,71%, tinggi busa 62,5% dan umur busa 315 menit.
Evaluation of Growth and Physiological Responses of Three Rice (Oryza sativa L.) Varieties to Elevated Temperatures Kurniawan, Dede Yudo; Junaedi, Ahmad; Lubis, Iskandar; Sunarti, Titi Candra
Journal of Tropical Crop Science Vol 6 No 01 (2019): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.944 KB)

Abstract

Temperature is a primary factor that affects the rate of plant development and has great impacts on plant growth, metabolism, and yield. A study was conducted to analyze the effects of elevated temperature on rice morphological and the physiological growth. The research was arranged in a nested randomized block design consisting of two factors, temperatures and rice varieties. Elevated temperatures were provided through the uses of different materials of plastic roof and walls to have an average and maximum temperature of 27.6 °C and 41.6 °C (T1); 28.1°C and 43.8 °C (T2), and 29.5°C and 47.1 C (T3), respectively. The study used three varieties of rice, “Ciasem”, “Ciherang”, and “IR64”. All rice varieties showed signifi cant increases in tiller number per hill and shoot dry weight, but had a decrease in the stomatal conductance, transpiration rate, and SPAD values at grain fi lling stage with the increasing temperatures. The number of tiller per hill increased when temperature was elevated from 27.6 to 28.1 and 29.5°C by about 29.9 and 21.3%, respectively.
Kemasan Antimikrob untuk Memperpanjang Umur Simpan Bakso Ikan Warsiki, Endang; Sunarti, Titi Candra; Nurmala, Lala
Jurnal Ilmu Pertanian Indonesia Vol 18, No 2 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Active packaging is a technique that can be developed to prolong shelf-life or enhance safety or sensory properties, while maintaining the quality of fresh products inside. This experiment produced active packaging of antimicrobial (AM) film in which an antimicrobial (AM) agent was added into the film (instead of food) and then used it to coat the food product. The AM agents slowly release into the product surrounds, inhibit the growth of microbes thus packing and preserving were done in a single step. Carragenan was chosen as a matrix film with garlic extract as an AM agent. Fish balls were chosen as a product to be coated by this AM film. The study consisted of 2 steps i.e.: (i) prepared AM film, and (ii) coated the fish balls with AM film and then tested the quality during storage. In general, the quality of fish balls before and after storage still meet the SNI 0-3819-1995. In the application, the fish balls with AM film has longer shelf life than that without AM. Further more, the result of organoleptic test on appearance of odor and color showed that the panelists prefered coated fish balls than the uncoated one. The panelist prefered coated fish ball which is stored at 5 and 15 °C. Keywords: antimicrobial packaging, fish ball, garlic extract  
Rekayasa Proses Hidrolisis Pati dan Serat Ubi Kayu (Manihot utilissima) untuk Produksi Bioetanol Susmiati, Yuana; Setyaningsih, Dwi; Sunarti, Titi Candra
Agritech Vol 31, No 4 (2011)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.9648

Abstract

Ethanol production from cassava (Manihot utilissima) usually uses enzymatic process for starch hydrolysis. Enzymatichydrolysis by α-amylase and amyloglucosidase enzymes are not able to convert cassava fibers into sugars. Dilute acid hydrolysis is applied to convert both starch and fibers, which will increase the yield of simple sugars as fermentable sugars and resulting in high ethanol production. In this research there are two steps of dilute acid hydrolysis, first for starch hydrolysis at H SO concentration of 0.1-0.5 M, 5-15 minutes and second for fiber hydrolysis at 0.5-1.0 24M H SO , 10-20 minutes, at the same temperature of 121-127 oC and pressure of 1.0-1.5 atm. The disadvantage of acid hydrolysis is the formation of toxic compounds such as hydroxymethyl furfural (HMF) which is inhibited yeast fermentation. Therefore, acid hydrolyzates were detoxified with NH OH before use as fermentation substrate. The best starch hydrolysis condition was obtained at 0.4 M H SO  for 10 minutes which gave 257.37 g/l of total sugars, 24229.38 g/l of reducing sugars, 89.59 of dextrose equivalent (DE) and 0.57 g/l of HMF. While the best fiber hydrolysis performed at 1.0 M H SO  solution for 20 minutes which gave 79.74 g/l of total sugars, 70.88 g/l of reducing sugars, 2488.99 of DE and 0.0142 g/l of HMF. Single direct acid hydrolysis was the most suitable substrate for yeast fermentationwith the ethanol concentration of 5.7 % (w/v) and 30.5 % (w/w) of ethanol yield. This result is comparable with enzymatic hydrolysis which gave ethanol yield of 30 % (w/w).ABSTRAKProduksi etanol dari ubi kayu biasanya menggunakan enzim untuk menghidrolisis pati. Hidrolisis secara enzimatismenggunakan enzim α-amilase dan amiloglukosidase tidak mampu mengkonversi serat menjadi gula. Hidrolisis asam  berkonsentrasi  rendah  dilakukan  untuk  mengkonversi  pati  dan  serat,  sehingga  gula-gula  sederhana  yang dapat difermentasi meningkat dan menghasilkan produksi etanol tinggi. Pada penelitian ini ada dua tahap hidrolisis menggunakan asam berkonsentrasi rendah, yaitu tahap pertama untuk menghidrolisis pati dengan konsentrasi H SO 0,1-0,5 M selama 5-15 menit dan tahap kedua untuk menghidrolisis serat dengan kensentrasi H SO  0,5-1,0 M selama 2410-20 menit pada suhu dan tekanan sama, yaitu 121-127 oC dan 1,0-1,5 atm. Kekurangan pada hidrolisis asam adalahterbentuknya senyawa toksik seperti hidroksimetil furfural (HMF) yang mengganggu fermentasi khamir. Oleh karena itu hidrolisat asam didetoksifikasi menggunakan NH OH sebelum digunakan sebagai substrat fermentasi. Kondisi terbaik hidrolisis pati diperoleh pada konsentrasi H SO  0,4 M selama 10 menit dengan nilai total gula 257,37 g/l, 24gula pereduksi 229,38 g/l, dextrose equivalent (DE) 89,59 dan HMF 0,57 g/l. Selain itu kondisi terbaik hidrolisis serat diperoleh pada konsentrasi H SO  1,0 M selama 20 menit dengan nilai total gula 79,74 g/l, gula pereduksi 70,88 g/l, 24DE 88,99 dan HMF 0,0142 g/l. Hidrolisat asam yang paling sesuai digunakan sebagai substrat fermentasi adalah darihidrolisis satu tahap tanpa pemisahan serat yang menghasilkan etanol dengan konsentrasi 5,7 % (b/v) dan rendemen etanol 30,5 (b/b). Hasil tersebut tidak berbeda jauh dengan hidrolisis enzimatis yang mengasilkan rendemen etanol 30% (b/b).
Inovasi Teknologi untuk Pengembangan Jagung dalam Rangka Mendukung Ketahanan Pangan dan Energi Hermawan, Wawan; santoso, Roh; Muhandri, Tjahja; Sunarti, Titi Candra
Jurnal Ilmu Pertanian Indonesia Vol 17, No 3 (2012): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Recent researches resulted several innovations such as an integrated machine for planting and fertilizer application for corn cultivation, corn noodle production, and delignification process of corn stover. The objectives of this research were (1) to improve working performances of the first prototype of integrated machine for tillage, planting, and fertilizer application for corn cultivation, (2) to develop an automatic irrigation system using solar energy, (3) to optimize processing variables on corn noodle production, and (4) to explore the delignification process of corn stover to improve the enzymes susceptibility in saccharification process. The planting and fertilizer application machine for corn cultivation has been successfully modified and showed better working performance. An automatic irrigation system using solar energy for corn cultivation has been arranged using a solar panel, a water pump, a battery, and a controlling system. The pumping discharge from a 10 m depth of well was 0.1 l/s. The optimum processing condition was resulted from the combination of 80% dough’s moisture content, at 90 °C extruder temperature and 75 rpm screw speed. The microwave heating treatment could destruct and solubilize the lignin, as compared to chemical and hydrothermal processes, and reduced the fiber crystallinity. Keywords: automatic irrigation, corn noodles, corn stover, delignification, planting machine