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Senyawa Aktif Sayuran Cruciferae dan Perubahan Kadar Kolesterol serta Vitamin C pada Tikus Hiperkholesterolemia Sunarsih, Endang Sri; Hakim, Lukman; Sugiyanto, Sugiyanto; Sumantri, Sumantri
MEDIA MEDIKA INDONESIANA 2011:MMI Volume 45 Issue 3 Year 2011
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

ABSTRACTActive compounds of cruciferae family and the change of total cholesterol level and vitamine C in hipercholesterolaemic ratsBackground: Cauli flower is a member of the Cruciferae family. One of the active compound content of indole-3-carbinol, is used to prevent and treat cancer. Vitamine C in the cauli flower as an antioxidant is able to avert free radicals that are formed in aerobic organisms and hipercholesterolaemic condition. The study aimed to determine the active compounds contained in the of cauli flower and its role in changing of the lipid profile and vitamine C levels in hipercholesterolaemic rats.Methods: To identify up the active compounds of cauli flower by maceration repeately, with alcohol: ammoniak (19:1). The results were determinated by thin layer chromatography (TLC). Spotting results obtained were compared with standard spots in its color reaction and Rf spots with Rf standard samples. Twenty rats were divided into 4 groups, and made hipercholesterolaemic, group I as a negative control, group II as a positive control were given green tea 0.605 g/kgBW, group III-IV were given cauli flower juice for 14 days dose of 10 and 15 ml/kgBW, the blood were analyzed for total cholesterol level and vitamine C plasma.Results: Cauli flower active compounds are indole-3-carbinol, indole-1-3-carbinol, indole-3-carboxylic acid and sulforafan they were capable of decreasing total cholesterol levels to 45%, and increasing plasma vitamine C level by 55% compared to before treatment.Conclusion: Cauli flower contains 4 indoles, and is able to decrease total cholesterol and increase vitamine C plasma in hipercholesterolaemic rats.Keywords: Cruciferae, indole, TLC, cholesterol, vitamine CABSTRAKLatar belakang: Bunga kubis, termasuk keluarga Cruciferae. Salah satu kandungan senyawa aktifnya indol-3-karbinol, mampu mencegah dan mengobati penyakit kanker. Vitamin C yang terdapat di dalam bunga kubis sebagai antioksidan mampu menangkal radikal bebas yang terbentuk pada organisme aerobik, kondisi hiperkolesterolemia. Penelitian ini bertujuan untuk mengetahui senyawa aktif yang terkandung dalam bunga kubis (Brassica Oleracea var Botrytis L) dan perannya dalam mengubah profil lipid dankadar vitamin C pada tikus hiperkolesterolemia.Metode: Untuk menyari senyawa aktif bunga kubis dilakukan macerasi berulang, dengan alkohol: ammoniak (19:1). Hasil penyarian dilakukan penetapan senyawa aktif secara kromatografi lapis tipis (KLT). Hasil bercak yang diperoleh dibandingkan dengan bercak standar dengan membandingkan reaksi warna dan Rf bercak sampel dengan Rf standar.Untuk mengamati efek farmakologi, 20 ekor tikus dibagi menjadi 4 kelompok, dibuat hiperkolesterolemia, kelompok I sebagai kontrol negatif, kelompok II sebagai kontrol positif diberi teh hijau 0,605 g/kgBB, kelompok III-IV diberi jus bunga kubis bunga selama 14 hari dosis 10 ml/kgBB dan 15 ml/kgBB, data darah dianalisis kadar kholesterol total serum dan vitamin C plasma.Hasil: Bunga kubis (Brassica Oleracea var Botrytis L) mengandung senyawa aktif: indol-3-karbinol, indol-1-3-karbinol, indol-3- asam karboksilat dan sulforafan yang mampu menurunkan kadar kolesterol total serum sampai 45% dan meningkatkan kadar vitamin C plasma sampai 55% dibanding sebelum perlakuan.Simpulan: Bunga kubis mengandung 4 senyawa indol dan mampu menurunkan kolesterol total serum dan meningkatkan vitamin C plasma pada tikus hiperkolesterolemia
PENGARUH PEMBERIAN INFUSA DAUN SALAM (Syzygium polyanthum(Wight) Walp) SEBAGAI ANTIDISENTRI PADA GAMBARAN HISTOPATOLOGI MUKOSA KOLON MENCIT BALB/c YANG DIINFEKSI Shigella flexneri Sunarsih, Endang Sri; Setyowati, Lusiana Elia; Sulistyarini, Indah
STIFAR - Sekolah Tinggi Ilmu Farmasi Vol 3, No 2 (2008)
Publisher : STIFAR - Sekolah Tinggi Ilmu Farmasi

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The aim of this research is to know the effect of salam leaf infusion (Syzygium polyanthum (Wight) Walp) to repair the tissue damage observed by histopathology profile of colon mucous on BALB/c mice which were infected by Shigella flexneri and to know the effective dosage of the salam leaf administration. The tested animal were divided in 6 groups. The first group as the positive control, were infected by Shigella flexneri, without medication. The 2nd group as the negative control, normal, healthy mice, without infected by Shigella flexraeri. The 3th group were infected by Shigella, flexneri and were given salam leaf infuse dosage 3.02g/kg BW. The 4th group were infected by Shigella flexneri and were given salam leaf infuse dosage 4.12g/kg BW. The 5th group were infected by Shigella flexneri and were given salam leaf infuse dosage 5.16g/kg BW. The 6th group as the comparative group were infected by Shigella flexneri, then they were treated with ciprofloxasin dosage 182mg/kg BW. The administiation of salam leaf infuse and the standard comparative medication of ciprofloxasin was started at the 4th day, for 5 days. At the 3th and 6th after the administration of salam leaf infuse and ciprofloxasin colon mucous histopathology test was conducted, then analyzed based on the Scoring Modified System For Shigellosis category. The result showed a significant different (p<0,05). Mann Whitney test was conducted to indicate which group showed the different. The result indicates that salam leaf infuse can cure the damaged colon mucous tissue on mice which were infected by Shigella flexneri with the third dosage as the effective dosage as much as 5.16 g/kg BW for 5 days medication.   Keyword: Shigella flexneri, salam leaf infuse, histopathology
PENGARUH PRAPERLAKUAN JUS KUBIS BUNGA (Brassica oleracea L. var botrytis L.) TERHADAP AKTIVITAS DIKLOFENAK DALAM TERAPI INFLAMASI Sunarsih, Endang Sri; Palupi, Dwi Hadi Setya; Hapsari, Indriati
Majalah Obat Tradisional Vol 16, No 1 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/mot-TradMedJ16iss1pp%p

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Terapi suatu obat yang bersamaan dengan konsumsi makanan dapat mempengaruhi efektivitas obat tersebut. Penelitian ini bertujuan untuk mengetahui pengaruh praperlakuan jus kubis bunga (Brassica oleracea L. var. botrytis L.) terhadap aktivitas diklofenak dalam terapi inflamasi pada tikus putih jantan galur Wistar. Tiga puluh ekor tikus dibagi enam kelompok secara acak. Kelompok I (kontrol negatif) hanya diberi aquadest, kelompok II (kontrol positif) diberikan perlakuan natrium diklofenak dosis 5,04 mg/kg BB secara peroral. Kelompok III diberi praperlakuan jus kubis bunga dosis 20 ml/kg BB 1 jam sebelum perlakuan natrium diklofenak. Kelompok IV diberi praperlakuan jus kubis bunga dosis 20 ml/kg BB 1 hari sebelum perlakuan natrium diklofenak. Kelompok V diberi praperlakuan jus kubis bunga dosis tunggal 20 ml/kg BB selama 3 hari sebelum perlakuan natrium diklofenak dan kelompok VI diberi praperlakuan jus kubis bunga dosis tunggal 20 ml/kg BB selama 5 hari sebelum perlakuan natrium diklofenak. Tiga puluh menit setelah perlakuan natrium diklofenak, kemudian telapak kaki tikus diinjeksi dengan karagenin 1% sebanyak 0,10 ml secara subplantar. Volume udema diukur dari jam ke-0 sampai jam ke-5 setelah injeksi karagenin menggunakan pletismometer. Hasil analisa statistik data AUC dan DAI menunjukkan bahwa praperlakuan jus kubis bunga yang diberikan 1 jam sebelum pemberian natrium diklofenak tidak memperlihatkan perubahan yang bermakna terhadap kontrol positif. Hasil penelitian ini menunjukkan bahwa praperlakuan jus kubis bunga selama 3 hari dan 5 hari mampu menurunkan daya antiinflamasi dari diklofenak, sehingga terapi inflamasi dengan diklofenak menjadi tidak maksimal.
Influence of administration of gadung corm ((Dioscorea hispida Dennst) infusion to decrease of blood glucose level at aloksan inducted male diabetic rats. Sunarsih, Endang Sri; ., Djatmika; Utomo, Retno Sri
INDONESIAN JOURNAL OF PHARMACY Vol 18 No 1, 2007
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.566 KB) | DOI: 10.14499/indonesianjpharm0iss0pp29-33

Abstract

Traditional herba medicine, Gadung (Dioscorea hispida Dennst) has already known can be used for oral anti diabetic drug. But it have not be proved experimentally. The purpose of experiment want to know the influence of Gadung (Dioscorea hispida Dennst) corm infuse, can decrease blood glucose consentration diabetic aloxan inducted rats.This experiment use 20 rats by randomized design at 2-3 months, 140-280 gram BW, Aloxan inducted diabetic male albino Wistar strain. The dose of aloxan 150 mg/BW given by intraperitonial. The rats divided in 4 groups, each group consist of 5 rats. First group : positif controle which is given insulin 12,6 UI/BW subcutanly; Second group : negative controle which is given aqua dest 5 mL/BW orally; Third group is given Gadung (Dioscorea hispida Dennst) corm infuse 630 mg/BW orally and Forth group is given Gadung (Dioscorea hispida Dennst ) corm infuse 1260 mg/BW dose orally. The experiment results of blood glucose level, was analyzed by 2 way ANOVA (p<0,05).The conclusion of the experiment is the rats has given Gadung (Dioscorea hispida Dennst) corm infuse 630 mg/BW and 1260 mg/BW can decrease blood glucose consentration proporsionally with insulin effect (p<0,05).Key words : Gadung (Dioscorea hispida Dennst), Aloksan, antidiabetic drug.
The effect of variation of dose, time toward cauli flower (Brassica oleracea var BotrytisL.) On hepatic cytochrome P-450 level rats given theophylline Sunarsih, Endang Sri; hakim, Lukman; ., Sugiyanto; ., Sumantri
INDONESIAN JOURNAL OF PHARMACY Vol 22 No 4, 2011
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.974 KB) | DOI: 10.14499/indonesianjpharm0iss0pp323-329

Abstract

Cytochrome  P-450  as  a  major  component  enzyme  system  in  drug metabolism. Activity  enzyme  of  cytochrome  P-450  was  influenced  by  internal and external factors. Vegetables of Brassicaceae such as cauli flower were often consumed  in  the  long  term,  its   has  inductor  activity  of  oxidation  enzyme systems and conjugation reactions. Theophylline as a bronchodilator drugs have the unknown effects on hepatic microsomal enzyme, such as cytochrome P-450 and   have  not  been  many  studies  that  tried  to  prove  it.  The  purpose  of  this study  was  to  prove  the  effects  of Cauliflower  and  indol  on  level  cytochrome  P-450  enzyme.  90  rats  were  divided  into  3  groups.  Group   I,  were  given Theophylline  20  mg/kg  BW.  In  group  II  30  rats  were  treated  with  indole  1,2; 2,4; 3,6 mg/kg BW, and group III 30 rats were treated with cauliflower extract respectively  doses  100,  200,  300  g/kg  BW.  Each  dose  was  given  on  10  rats, each group were divided 2 sub-groups were treated for 5, 10 days. On the last day  of  treatment  were  given  Theophylline  20  mg/kg  BW.   Cytochrome  P-450 enzyme  levels  were  determined  by  the  method  of  Omura  and  Sato(Snell,  and Mullock,1987). An induction cauliflower and indole did not increased levels of hepatic  cytochrome  P-450.  The  long  treatment  and  the   increased  of administered  dose  did  not  enhanced  the  levels  of  hepatic  cytochrome  P-450 enzyme. cauliflower and indole contained in vegetables when consumed together with theophylline drug, would not affect the metabolism of theophyllineKey words: Cauli flower, cytochrome P-450, theophylline, indole, spectrofotometric.
The effect of cauli flower (Brassica oleracea var. botrytis L.) to the change of : cholesterol, MDA level of rats hiperlipide Sunarsih, Endang Sri; S., M. Caecilia Nanny; Fajaryanti, Nita
INDONESIAN JOURNAL OF PHARMACY Vol 20 No 1, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.885 KB) | DOI: 10.14499/indonesianjpharm0iss0pp35-41

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The unbalance of oxidant and antioxidant can effect of various diseases, for example atherosclerosis. High total cholesterol level was given the risk factor to atherosclerosis. This research aims to know the effect of cauliflower (Brassica oleracea var. botrytis L.) juice to change of total cholesterol level and peroxide of lipid (MDA level). A twenty white rats Wistar strain were treated as hiperkolesterolemia with consumed a high fat meal for 14 days, then devided in to 4 groups. Group I as positive control was given a green tea leaf infuse through a dosage 0,605 g/ kg BW, group II as negative control was given an aquadestilata. Then Group III and IV as were given cauliflower juice through a dosage 15 mL/ kg BW and 20 mL/ kg BW for 14 days. The result of this research were concluded that cauliflower juice through a dosage 15 mL/ kg BW and 20 mL/ kg BW was decreased of total cholesterol level and lipid peroxide (MDA level). Cauliflower juice through a dosage 20 mL/kg BW was found as an effective dosage to decreased of total cholesterol level and lipid peroxide (MDA level) of male white rats Wistar strain with high fat diet treatment.Key words : Juice, cauliflower, total cholesterol, peroxide of lipid, MDA.
PENGARUH PEMBERIAN MINYAK JELANTAH TERHADAP PROFIL FARMAKOKINETIK PARASETAMOL PADA TIKUS WISTAR Kusuma, Faizurrahman Andi; Sunarsih, Endang Sri; Annisaa, Eva
JURNAL KEDOKTERAN DIPONEGORO Vol 5, No 4 (2016): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Jurusan Kedokteran Umum, Fakultas Kedokteran, Universitas Diponegoro

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Latar Belakang : Penggunaan minyak goreng yang telah dipanaskan berulangkali banyak dilakukan di masyarakat untuk menekan pengeluaran biaya dalam memasak. Minyak goreng yang dipanaskan dan digunakan berulang akan membentuk suatu radikal bebas yang dapat menjenuhkan antioksidan endogen, yaitu gluthation. Parasetamol mempunyai efek analgesik dan antipiretik yang banyak digunakan masyarakat. Dalam metabolisme parasetamol, gluthation mempunyai peran yang penting.Tujuan : Mengetahui pengaruh minyak jelantah terhadap profil farmakokinetik parasetamol dalam darah tikus wistarMetode : True experimental dengan post test only control group design. Setelah diadaptasi dengan diet standar selama 7 hari, 14 ekor tikus wistar jantan dibagi menjadi 2 kelompok. Kelompok K sebagai kelompok kontrol, dan kelompok P sebagai kelompok perlakuan. Kelompok K diberi diet standar dan kelompok P diberi diet minyak jelantah ad libitum selama 56 hari. Pada hari ke-57 semua tikus diberi parasetamol oral 12,5 mg/200gramBB. Pengambilan cuplikan darah dilakukan dari vena retroorbita pada menit ke-3, 5, 10, 20, 30, 40, 60, 90, 120, 180, 240, 300, dan 360. Kadar parasetamol dalam plasma diukur dengan spektrofotometer UV pada panjang gelombang 242 nm.Hasil : Uji statistik dengan uji t tidak berpasangan menunjukkan perbedaan bermakna pada parameter Cl, Kel, t1/2e, Cmaks, AUC (p<0,05) dan menunjukkan perbedaan tidak bermakna pada parameter Ka,Vd, tmaks (p>0,05).Simpulan : Minyak jelantah dapat mempengaruhi profil farmakokinetik parasetamol dengan meningkatkan parameter t1/2e, Cmaks, AUC dan menurunkan parameter Cl, dan Kel.
Protective effect of Brassica oleracea var. botrytis L. against theophylline-induced hepatocellular abnormalities in rats Sunarsih, Endang Sri; Hakim, Lukman; Sugiyanto, Sugiyanto; Sumantri, Sumantri
Universa Medicina Vol 31, No 1 (2012)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2012.v31.12-19

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BackgroundIndole and its derivatives, such as indole acetic acid are active constituents of cauliflower (Brassica oleracea var. botrytis L.) with are known to function as plant growth hormones. Drug metabolism mainly occurs in the liver, so that the possibility of damage to this organ is considerable. The purpose of this study was to evaluate the effect of cauliflower extract on alanine transaminase (ALT) levels and on the hepatocyte morphology induced by theophylline in Wistar rats.MethodsTwenty Wistar rats were divided randomly into four groups. Group I was given only theophylline 20 mg/kg BW. Groups II, III, and IV were given cauliflower extract 200 g/kg BW, for 5, 10, 15 days, and on the last day were given theophylline 20 mg/kg BW. On days 5, 10, and 15 the animals were terminated, their livers taken and observed for microscopic appearance, while blood samples were taken for measuring ALT levels.ResultsCauliflower affected a reduction in ALT levels, mean serum ALT concentration in rats given cauliflower for 5 days (109.86 ± 2.20 IU/L was significant higher compared to rats given cauliflower for 15 days (71.58 ± 3.17 IU/L) (p=0.000). Mean values of histopathologic liver cells in rats given cauliflower for 15 days (1.17 ± 0.01) significant lower compared to rats given cauliflower for 5 days (1.45 ± 0.06) (p=0.000).ConclusionSCauliflower extract reduced the level of ALT and improved the microscopic appearance of hepatic cells in the rats. Cauliflower extract could lead to the development of potent anti-tumor agents for hepatic cancer treatment.
Upaya Meningkatkan Kinerja Melalui Kepuasan Kerja (Studi pada Kantor Kesyahbandaran dan Otoritas Pelabuhan Kelas IV Tegal) Suparjo, Suparjo; Sunarsih, Endang Sri; Santoso, Kadar
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Publisher : UNTAG Semarang

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Penelitian ini bertujuan untuk mengetahui  alternatif upaya untuk meningkatkan kinerja pegawai  Kantor Kesyahbandaran dan Otoritas Pelabuhan Kelas IV Tegal melalui kepuasan kerja pegawai. Untuk keperluan tersebut  dilakukan analisis pengaruh kompensasi, motivasi, dan kepuasan kerja , terhadap kinerja pegawai.  Populasi penelitian ini adalah seluruh pegawai pada Kantor Kesyahbandaran dan Otoritas Pelabuhan Kelas IV Tegal sebanyak 44 pegawai dan sekaligus dijadikan sebagai  sampel penelitian. Teknik penentuan sampel menggunakan sampling jenuh atau sensus. Teknik analisis yang digunakan adalah Regresi Linier Berganda.Uji hipotesis yang digunakan adalah Uji T dan Uji Mediasi.Hasil penelitian mwnunjukkan bahwa kompensasi berpengaruh positif dan signifikan berperhadap kinerja pegawai, kompensasi berpengaruh positif dan signifikan terhadap kepuasan kerja pegawai, motivasi berpengaruh positif dan signifikan terhadap kinerja pegawai, motivasi berpengaruh positif dan signifikan terhadap kepuasan kerja pegawai, kepuasan kerja berpengaruh positif dan signifikan terhadap kinerja pegawai.Kata kunci : kompensasi, motivasi, kepuasan kerja, kinerja pegawai AbstractThis study aims to find out the alternative efforts to improve the performance of the employees of the Class IV Port Authority and Harbormaster Office through employee job satisfaction. For this purpose an analysis of the effect of compensation, motivation and job satisfaction is carried out on employee performance. The population of this study were all employees of the Tegal Class IV Port Authority and Port Authority and Authority as many as 44 employees and at the same time used as research samples. Sample determination technique uses saturated sampling or census. The analysis technique used is Multiple Linear Regression. The hypothesis test used is the T Test and Mediation Test. The results showed that compensation had a positive and significant effect on employee performance, compensation had a positive and significant effect on employee job satisfaction, motivation had a positive and significant effect on employee performance, motivation had a positive and significant effect on employee job satisfaction, job satisfaction had a positive and significant effect on performance employee.Keywords: compensation, motivation, job satisfaction, employee performance
Pengaruh Pemberian Vitamin C Terhadap Kadar Kolesterol Total Serum Tikus Jantan Hiperlipidemia Setelah Perlakuan Jus Lidah Buaya (Aloe vera Linn) Sunarsih, Endang Sri; Pamuji, Slamet
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 05 No. 02 Agustus 2007
Publisher : Fakultas Farmasi, Universitas Muhammadiyah Purwokerto

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Penelitian ini bertujuan untuk mengetahui pengaruh pemberian vitamin C terhadap kadar kolesterol total serum tikus hiperlipidemia yang telah di beri jus Aloe vera. Linn. Penelitian dilakukan dengan desain Pre and Post Randomized Controlled  Group Design, menggunakan tikus Wistar yang telah dibuat hiperlipidemia. Tikus diberi jus Aloe vera Linn dengan dosis 4 ml/kg BB/hari selama 15 hari. Kemudian dilanjutkan pemberian vitamin C selama 3 hari dengan dosis 3,38 mg/kg BB dan 11,25 mg/kg BB. Pemeriksaan kadar kolesterol total serum menggunakan metode  CHOD-PAP. Data yang diperoleh dianalisis menggunakan uji t berpasangan dan anova pada derajat kemaknaan  5%. Hasil penelitian menunjukkan bahwa pemberian vitamin C secara bermakna menurunkan kadar kolesterol total serum pada tikus hiperlepidemia yang telah diberi jus  Aloe vera. Linn. Peningkatan dosis vitamin C berpengaruh secara bermakna dalam menurunkan kadar kolestrol total serum tikus.