Siti Sunarintyas
Bagian Biomaterial, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia

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Effect of Bombyx mori silk-fiber volume on flexural strength of fiber-reinforced composite Fransiska, Aria; Sunarintyas, Siti; Dharmastiti, Rini
Majalah Kedokteran Gigi Indonesia Vol 4, No 2 (2018): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (489.826 KB) | DOI: 10.22146/majkedgiind.25186

Abstract

Dental glass fiber is one of dental synthetic fibers that are widely used in dentistry as a dental resin reinforcement, such as in dentin replacement material. The availability of glass fiber is limited in Indonesia because it must be imported and relatively expensive. Bombyx mori silk-fiber is one of the strongest natural fiber derived from silkworm cocoon processing. Silk-fiber is used in medical applications as a post-surgical sutures, scaffolds for tissue engineering and drug delivery. The purpose of this study was to evaluate the effect of Bombyx mori silk-fiber volume on the flexural strength of fiber-reinforced composite (FRC). We used Bombyx mori silk-fiber (Perhutani Pati, Central Java, Indonesia) and flowable composite (Charmfil flow, Denkist, Korea) in this study. The FRC samples were divided into 4 groups consisting of fiber volumes of 0%, 5%, 10% and 15% (n = 4). Tests of flexural strength were performed according to ISO 4049. The results were analyzed using one way ANOVA (p<0.05). The study showed that the means of the flexural strength (MPa) of Bombyx mori silk-fiber FRC for volume of 0%, 5%, 10% and 15% were 149.2 ± 5.5; 127.6 ± 3.8; 110.9 ± 3.5; 71.2 ± 4.2. One-way ANOVA test showed that the means of FRC flexural strength on the four groups’ silk-fiber Bombyx mori volumetric were significantly different (p<0.05). This study concluded that Bombyx mori silk-fiber volumetric influences the flexural strength of fiber- reinforced composite. An increase in Bombyx mori silk-fiber volume decreases the flexural strength of FRC because there is a small gap due to the weak interfacial bonds between dental flowable composite and Bombyx mori silk-silk-fiber.
EFEK APLIKASI PADA HIDROKSIAPATIT TERHADAP PERLEKATAN SEL OSTEOBLAS Sunarintyas, Siti
Jurnal Teknosains Vol. 1 No. 1 tahun 2011
Publisher : Sekolah Pascasarjana UGM

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Abstract

Hydroxyapatite (HA) is often used as a human bone graft. Modification of HA surface is developed to increase osseointegration process which is influenced by cells attachment surrounded the tissue. Sericin has polar side groups which accelerate cells attachment. The aim of this research was to determine the effect of sericin application over HA surface on the attachment of osteoblast cells. The research used HA synthesized from gypsum (Kulon Progo, Yogyakarta). Sericin was extracted from silkworm cocoons of Bombyx mori. Sericin of 0.01, 0.05, 0.1% were applied over HA discs surface (10 mm in diameter, 3 mm thick). The discs were inserted into osteoblast cells culture of MC3T3E1 for attachment test. The data were analyzed by ANOVA and LSD. The result showed that the amount of osteoblast cells attached to HA (7.60±0.42) was significantly difference (p&lt;0.05) with HA + sericin 0.01% (10.30±0.52), HA + sericin 0.5% (10.63±0.70), HA + sericin 0.1% (10.67±0.87). Variation of sericin concentrations applied over HA did not influence any significant difference on the amount of cells attachment (p&gt;0.05). In conclusion, sericin application over HA surface increased the amount of osteoblast cells attachment. Concentrations of sericin application over HA (0.01, 0.05, 0.1%) did not influence osteoblast cells attachment.
PENGARUH ORIENTASI DAN VOLUMETRIK GLASS FIBER NON DENTAL TERHADAP KEKUATAN PERLEKATAN GESER FIBER REINFORCED COMPOSITES UNTUK SPLINTING PERIODONTAL Sumantri, Dedi; Sunarintyas, Siti; Herawati, Dahlia
B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah Volume 2, Nomor 1, Juni 2015
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.474 KB) | DOI: 10.33854/JBDjbd.5

Abstract

Fiber Reinforced Composite (FRC) menggunakan E-glass fiber dental dalam bidang kedokteran gigi telah banyak diaplikasikan secara klinis, salah satunya dalam aplikasi splinting periodontal. Ketersediaan E-glass fiber dental di Indonesia masih terbatas dengan harga relatif mahal. Terdapat glass fiber non dental di Indonesia yang banyak digunakan bidang teknik, dengan harga terjangkau. Tujuan dari penelitian ini untuk mengetahui pengaruh orientasi dan volumetrik glass fiber non dental terhadap kekuatan perlekatan geser FRC pada splinting periodontal. Bahan penelitian berupa glass fiber non dental yang terdiri dari tiga jenis yaitu glass fiber mats (LT, China), glass fiber roving (CMAX, China), dan glass fiber woven roving (HJ, China), serta E-glass fiber dental (Fiber-Splint, Polidentia SA, Switzerland). Objek dibagi menjadi 8 kelompok, masing-masing kelompok terdiri atas 4 sampel. Kelompok I (kontrol) tanpa penambahan glass fiber non dental, kelompok II E-glass fiber dental 2,8 vol%, dan kelompok III - VIII terdiri dari glass fiber non dental mats, glass fiber non dental roving, dan glass fiber non dental woven roving, dengan fraksi volumetrik 2,8 dan 5,4 vol%. Seluruh sampel direndam dalam air destilasi selama 24 jam pada suhu 37o C. Pengujian kekuatan perlekatan geser menggunakan Universal Testing Machine. Data dianalisis menggunakan ANAVA dua jalur, uji t dan LSD. Hasil penelitian menunjukkan rerata kekuatan perlekatan geser (MPa) terendah pada kelompok tanpa fiber (kontrol) (8,95 ± 0,95) dan tertinggi pada 5,4 vol% glass fiber non dental woven roving (14,11 ± 0,35). Hasil analisis ANAVA dua jalur menunjukkan variabel orientasi dan volumetrik glass fiber non dental memberikan pengaruh signifikan (p<0,05). Uji post hoc LSD dan uji t menunjukkan perbedaan signifikan (p<0,05) pada seluruh kelompok perlakuan untuk variabel orientasi dan volumetrik. Kesimpulan penelitian ini adalah orientasi dan volumetrik glass fiber non dental memberikan pengaruh terhadap peningkatan kekuatan perlekatan geser FRC.
LAMA PEMBERSIHAN EFISIEN PAPAIN PADA PENGLEPASAN PLAK GIGITIRUAN Sunarintyas, Siti
Journal of Dentistry Indonesia Vol 10, No 1 (2003): APRIL
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (501.334 KB) | DOI: 10.14693/jdi.v10i2.424

Abstract

Everyday denture cleaning is needed to prevent the mouth from denture stomatitis and esthetic concern. Enzyme cleansers are developed in attempt to break down the organic components of denture plaque. The aim of this research is to determine the efficient cleansing duration of papain solution to remove 24-hour denture plaque. The research was done on 16 patients with complete dentures. Plaque removal was determined by Lowry method and SDS PAGE. The result shows that papain dose required to hydrolyze 24-hour dentue plaque is 15.66 TU/mg enzyme activity for 10 minutes soaking. Soaking the denture in papain for 10 minutes makes all of the detected plaque protein bands remobe. In conclusion, efficient cleansing duration of papain solution to remove 24-hour denture plaque is papain activity of 15.66 TU/mg by soaking duration of 10 minutes. Further research is suggested to examine papain residue on the denture that may influence the denture wearer biocompatibility.
HUBUNGAN ANTARA TEGANGAN PERMUKAAN RESIN AKRILIK DAN JUMLAH PELIKEL YANG TERADSORPSI Irnawati, Dyah; Sunarintyas, Siti
Journal of Dentistry Indonesia Vol 7, No 1 (2000)
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.12 KB) | DOI: 10.14693/jdi.v7i1.521

Abstract

The aim of this research is to investigate the correlation of acrylic resins surface tension (chemial, heat, and microwave activated) on the amount of adsorbed pellicle on the acrylic surfaces. Sixty samples (10x10x2 mm)  were made of chemical activated (Hilon, England), heat activated (Stellon, England), and microwave activated (Acron MC, Japan) acrylic resins. Contact angle (surface tension of acrylic resins and saliva (5 treatment groups, n=3) an acrylic resins and aquadest (control group, n=5) were measured by photographing method. The samples from treatment groups were contacted with saliva for 1 hour, 2, 3, 4 and 5 hours and the protein concentration were measured by Bradford method. The data were analyzed by analysis of variants, LSD, and correlations. The results showed that: (1) acrylic resins type influenced the surface tensions (p&lt;0,01), (2) the contact duration influenced the pellicle concentrations (p&lt;0,01), and (3) there was not any correlation between acrylic resins surface tensions and the concentration of adsorbed pellicle (p&gt;0,05). It was concluded that there is not any correlation between acrylic resins surface tension and the amount of adsorbed pellicle.
KARAKTERISASI PELIKEL YANG TERADSORPSI PADA PERMUKAAN RESIN AKRILIK Sunarintyas, Siti; Irnawati, Dyah
Journal of Dentistry Indonesia Vol 7, No 1 (2000)
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.917 KB) | DOI: 10.14693/jdi.v7i1.522

Abstract

This research was aimed to determine the composiiton of adsorbed pellicle on acrylic resin surface. Fifteen plates of 10x10x2 mm were made from chemical, heat, and microwave activated acrylic resin. The plates were incubated in saliva for 1,2,3,4, and 5 hours. Pellicle characterization was done every hour by SDS PAGE electrophoresis. The result showed that: 1. chemical, heat, and microwave activated acrylic resin adsorbed salivary pellicle by molecular weight of 50-61 kDa (amylase band); 2. incubation of 1,2,3,4, and 5 hours in saliva resulted in similar pellicle composition. In conclusion, adsorbed salivary pellicle on acrylic resin surface during 5 hours showed similar characteristic in every hour, with the major protein adsorbed: amylase.
Efektivitas Desensitizing Agent dengan dan tanpa Fluor pada Metode in Office Bleaching terhadap Kandungan Mineral Gigi (Kajian In Vitro) Kristanti, Yulita; Asmara, Widya; Sunarintyas, Siti; Handajani, Juni
Majalah Kedokteran Gigi Indonesia Vol 21, No 2 (2014)
Publisher : Majalah Kedokteran Gigi Indonesia

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Abstract

In office bleaching menggunakan hidrogen peroksida 40% sering memberikan efek samping berupa linu baik selama maupun setelah perawatan tersebut dilakukan. Penelitian ini dilakukan dengan tujuan untuk mengetahui pengaruhwaktu aplikasi dan kandungan fluor desensitizing agent pada metode in office bleaching terhadap kandungan mineral gigi. Delapan gigi masing-masing dipotong menjadi 4 bagian, 6 potong digunakan untuk pemeriksaan XRD awal. Gigi dikeringkan dalam oven suhu 50° selama 30 menit diserbuk, diambil 1 mg untuk diperiksa kandungan mineralnya dengan goniometer. Dua puluh empat potong yang lain dibagi dalam 4 kelompok perlakuan. Kelompok I: gigi diaplikasi bahan bleaching 0,5-1 mm hidrogen peroksida 40% selama 1 jam, dicuci, dikeringkan, diikuti aplikasi 0,1 ml desensitizing agent tanpa fluor (CPP-ACP) selama 30 menit, dicuci, dikeringkan. Kelompok II : gigi diaplikasi CPP-ACP 30 menit, dicuci, dikeringkan, dibleaching menggunakan hidrogen peroksida 40% selama 1 jam. Selanjutnya gigi diaplikasi CPP-ACP 30 menit lagi, dicuci, dikeringkan. Kelompok III gigi dibleaching menggunakan hidrogen peroksida 40% selama 1 jam, dicuci, dikeringkan, diikuti aplikasi desensitizing agent yang mengandung fluor (CPP-ACFP) selama 30 menit, dicuci, dikeringkan. Kelompok IV: gigi diaplikasi CPP-ACFP 30 menit, dicuci, dikeringkan, dibleaching menggunakan hidrogen peroksida 40% selama 1 jam. Selanjutnya gigi diaplikasi CPP-ACFP selama 30 menit, dicuci dikeringkan. Sesudah perlakuan, semua gigi dilakukan pemeriksaan kandungan mineral gigi dengan prosedur yang sama. Uji Mann Whitney menunjukkan penurunan mineral paling sedikit terjadi pada kelompok IV (4500). Desensitizing agent mengandung F sebelum dan sesudah perlakuan in office bleaching menunjukkan penurunan mineral paling kecil. ABSTRACT: The Effectiveness of Desensitizing Agent with and without Fluorine in Office Bleaching Method to Tooth Mineral content. Tooth sensitivity arises during or after an in-office bleaching was performed is usually overcome by using desensitizing agent with or without fluor. So far, desensitizing method applied only reduces tooth sensitivity but it had not overcome demineralization problem yet. This study was aimed to determine the influence of an applicationprocess and fluoride containing desensitizing agent in tooth mineral lossEight teeth were divided into four parts. Six specimens were smoothened using agate mortar. One mg of powder was inserted into the sample holder and mounted ongoniometer heads to examine mineral content before treatment using X-Ray Diffraction (XRD). Twenty-four specimens were classified into four groups. In group I, 0,1 ml desensitizing agent without fluoride (CPP-ACP) was applied for 30 minutes after an in-office bleaching using 40% Hydrogen Peroxide was performed, while in group II, CPP-ACP was applied before and after an in-office bleaching was performed. In group III 0,1 ml fluoride containing desensitizing agent (CPP-ACFP) was applied for 30 minutes after in-office bleaching using 40% Hydrogen Peroxide was performed, while in group IV fluoride containing desensitizing agent was applied before and after an in-office bleaching was performed. Finally, all of the specimens were measured in their after-treatment mineral contain. Mann Whitney test showed that the lowest mineral lost was detected in group IV (4500). Fluoride containing desensitizing agent applied before and after an in-office bleaching effectively reduced mineral loss.
Anti-Inflammatory Activity of Ethanol Extract of Cashew Stem Bark (Annacardium Occidentale L.) on Rat Paw Edema Induced by Carrageenan Harsini, Harsini; Sutardja, Iwa; Martono, Sudibyo; Sunarintyas, Siti; Sudarsono, Sudarsono
The Indonesian Journal of Dental Research Proceeding Book
Publisher : The Indonesian Journal of Dental Research

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (19.124 KB)

Abstract

Introduction: Cashew stem bark (Anacardium occidentale L.) was traditionally used to cure inflammation in the oral cavity. Phenolic substances such as phenol and anacardic acid that have anti-inflammatory effect was found in cashew stem bark. The aim of this study was to investigate the anti-inflammatory activity of the ethanol extract of cashew stem bark and indometazine as Non-steroid anti-inflammatory drug. Materials and Methods: Cashew stem bark was collected from Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia. Extraction was done by maceration method using ethanol as solvent. Anti-inflammatory activity of 40 mg/kg bw, 80mg/kg bw, 160mg/kg bw dosage of cashew stem bark extract was monitored and indometazine 10 mg/kg bw was used as positive control. Edema volume determination on rat paw was counted as area under cure (AUC) value and anti-inflammatory percentage. Result: This study result showed that total phenolic content on cashew stem bark was 12.25 ± 0.26% w/w gallic acid equivalent (GAE). The anti-inflammatory activity of cashew stem bark extract in this study were 9.985±6.483% for 40mg/kg BW, 15.576±6.754% for 80mg/kg bw, 25.87±19.7% for 160mg/kg bw and 56.85 ±15.52% for Indometazine 10 mg/kg bw. Analysis of Variance (ANOVA) method was applied on the results and showed significant anti-inflammatory activity of ethanol extract on cashew stem bark (p&lt;0.05). Conclusion: In conclusion, ethanol extract of cashew stem bark has anti-inflammatory activity. However, its’ activity is lower than indometazine.
Color stability of visible light cured composite resin after soft drink immersion Hasan, Alizatul Khairani; Sunarintyas, Siti; Irnawati, Dyah
Dental Journal (Majalah Kedokteran Gigi) Vol 42, No 3 (2009): (September 2009)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (227.059 KB) | DOI: 10.20473/j.djmkg.v42.i3.p123-125

Abstract

Background: Composite resin is a tooth-colored filling material containing Bis-GMA which exhibits water sorption properties. People tend to consume soft drink with various colors. Water sorption properties can alter the color stability of composite resin purpose. Purpose: This study was to determine the influence of immersion durations of composite resin in soft drink on color stability. Methods: The visible-light cured hybrid composite resin and soft drink were used. Ten disk specimens (2.5 mm thickness and 15 mm diameter) of composite resin were prepared and light cured for 20 seconds, then stored in distilled water for 24 hours at 37° C. The initial color of specimens were measured by Chromameter. After that, each specimen was immersed in 30 ml of soft drink up to 48, 72, and 96 hours at 37° C. The specimens’ color were measured again after each immersion. The color changes were calculated by CIE L*a*b* system formula. The data was analyzed by One-Way ANOVA and LSD (α = 0.05). Result: The ANOVA showed that the immersion durations of composite resin in soft drinks had significant influence on the color stability (p < 0.05). The LSD0.05 tests showed significant differences among all groups. The least color change was detected from the group of 48 hours immersion, while the greatest color change was from the group of 96 hours immersion. Conclusions: The immersion of composite resin in soft drinks influenced the color stability (began after 48 hours immersion).
EFEK APLIKASI SERICIN PADA HIDROKSIAPATIT TERHADAP PERLEKATAN SEL OSTEOBLAS Sunarintyas, Siti; Yustisia, Yeni; Tontowi, Alva Edy
Jurnal Teknosains Vol 1, No 1 (2011): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2056.833 KB) | DOI: 10.22146/teknosains.3989

Abstract

Hydroxyapatite (HA) is often used as a human bone graft. Modification of HA surface is developed to increase osseointegration process which is influenced by cells attachment surrounded the tissue. Sericin has polar side groups which accelerate cells attachment. The aim of this research was to determine the effect of sericin application over HA surface on the attachment of osteoblast cells. The research used HA synthesized from gypsum (Kulon Progo, Yogyakarta). Sericin was extracted from silkworm cocoons of Bombyx mori. Sericin of 0.01, 0.05, 0.1% were applied over HA discs surface (10 mm in diameter, 3 mm thick). The discs were inserted into osteoblast cells culture of MC3T3E1 for attachment test. The data were analyzed by ANOVA and LSD. The result showed that the amount of osteoblast cells attached to HA (7.60±0.42) was significantly difference (p<0.05) with HA + sericin 0.01% (10.30±0.52), HA + sericin 0.5% (10.63±0.70), HA + sericin 0.1% (10.67±0.87). Variation of sericin concentrations applied over HA did not influence any significant difference on the amount of cells attachment (p>0.05). In conclusion, sericin application over HA surface increased the amount of osteoblast cells attachment. Concentrations of sericin application over HA (0.01, 0.05, 0.1%) did not influence osteoblast cells attachment.