Articles

Found 14 Documents
Search

INTERVENSI CYTOADHERENCE SEBAGAI PELUANG UNTUK PENCEGAHAN DAN TERAPI MALARIA BERAT

Jurnal Kedokteran Brawijaya Vol 22, No 2 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Original Source | Check in Google Scholar

Abstract

Abstract  In  malarial  infection,  erythrocytes  infected  wih  Plasmodium  falciparum  bind  to  endothelial  vascular  (endothelial cytoadherence). This binding is implicated in the forming of sequestre and  rosette that affects the vascular circulation, and thus injures  the capillary wall. This mechanism is important in pathogenesis of malarial due to dysfunction of several organs. There are several receptors of  cytoadherence in human e.g Thrombospondine (TSP), CD-36, ICAM-1, and ELAM-1 as well as specific ligands of the parasite  e.g  Plasmodium falciparum Erythrocyte Membrane Protein-1 (PfEMP-1), 220 kDa protein of Pf60, Pf332, sequestrin, Pfaldhesin and STEVOR.  PfEMP-1 has been revealed as a molecule that is responsible for pathogenesis of severe malaria. This protein can pass parasitophorous vacoular membrane (PVM) of the parasite by attaching its molecule to carry Protein Export Elemen (PEXEL) and than go to the surface of erythrocytes in combination with specific helper protein in maeurer cleft.  The intervention on cytoadherence process through blocking of specific ligand directly or blocking the translocation of this ligand to the surface of erythrocytes might be important in regulating the outcome of malarial infection.  Key words : Cytoadherence, severe malaria, intervention, adhesion molecule.

Karakteristik Atom Mineral pada Osteoporosis dengan Arsitektur Porosis dan Nonporosis

Majalah Kedokteran Bandung Vol 45, No 1 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Original Source | Check in Google Scholar

Abstract

Terdapat hasil yang bervariasi dalam peningkatan bone mineral density (BMD) akibat asupan kalsium mengindikasikan keterlibatan atom mineral lain dalam perkembangan struktur tulang. Kuantitas atom mineral tulang yaitu kadar atom mineral dalam struktur tulang. Penelitian ini bertujuan untuk membandingkan karakteristik atom mineral pada gambaran arsitektur (scanning electron microscope/SEM) porosis dibandingkan dengan nonporosis. Sepuluh penderita osteoporosis dengan gambaran SEM porosis dan tujuh penderita gambaran SEM nonporosis terlibat dalam penelitian ini. Tulang yang diambil ketika operasi fraktur dianalisis mikrostruktur dengan SEM dan karakter atom mineral tulang dengan X-ray fluorescence (XRF). Pemeriksaan SEM dan XRF dilaksanakan di Laboratorium Fisika Universitas Negeri Malang. Penelitian dilakukan mulai Agustus 2010 sampai Januari 2011. Uji t dilakukan untuk menentukan perbedaan kadar mineral tulang. Hasil SEM menunjukkan perbedaan porositas dan integritas trabekular antara SEM porosis dan SEM nonporosis. Analisis XRF didapatkan komposisi atom yang lebih tinggi pada tulang SEM nonporosis yaitu P, S, Fe, Ni, Cr, Pb, dan Si. Sebaliknya, komposisi atom yang lebih tinggi pada tulang SEM porosis adalah Cu, Zn, Ca, dan Mo. Perbedaan komposisi atom tersebut tidak berbeda secara bermakna (p>0,05). Simpulan, perkembangan porositas pada osteoporosis tidak didasari oleh kuantitas atom mineral tulang. [MKB. 2013;45(1):23–7]Kata kunci: Arsitektur, atom mineral, komposit, osteoporosisMineral Atomic Characteristic on Porotic and Non Porotic Architecture  of OsteoporosisThere was variance result in increased bone mineral density (BMD) due to calcium intake which indicates involvement of another mineral atomic in bone structure development. Bone mineral atomic quantity is the level or concentration of mineral atomic in bone structure. Aim of this study was to compare mineral atomic characteristic in architecture (scanning electron microscope/SEM) porotic and non porotic figure. Ten osteoporosis patients with porotic SEM and seven non porotic SEM patients were involved in this study. Bone which was obtained from fracture surgery was analyzed for microstructure by SEM and for bone atomic mineral characteristic by X-ray fluorescence (XRF). Analysis of SEM and XRF were done in Physics Laboratory State University of Malang. This study was done from August 2010 to January 2011. t-test was done to analyse the difference of atomic mineral level. Scanning electron microscope result showed the difference in porosity and trabecular integrity between porotic and non porotic SEM. Analysis of XRF showed atomic composition was higher in porotic SEM such as P, S, Fe, Ni, Cr, Pb and Si. Conversely atomic composition such as Cu, Zn, Ca, and Mo, was higher in non porotic SEM. There was no significant difference between each groups (p>0.05). In conclusion, the development of porocity in osteoporosis is not depend on bone mineral atomic quantity. [MKB. 2013;45(1):23–7]Key words: Architecture, composite, mineral atomic, osteoporosis DOI: http://dx.doi.org/10.15395/mkb.v45n1.96

Detection of GAD65 autoantibodies of type-1 diabetes using anti-GAD65-abs reagent produced from bovine brain tissue

Medical Journal of Indonesia Vol 14, No 4 (2005): October-December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (296.419 KB)

Abstract

Clinically, type 1 diabetes may presents as type 2 diabetes which sometimes not easily differentiated. Perhaps only autoimmune markers of β-cells destruction could differentiate those two clinical conditions. Due to extremely high cost ( $ 150/test), examination of anti-glutamic acid decarboxylase-65 auto-antibodies (anti-GAD65Abs) may not be routinely performed in most, if not all, clinical laboratories in Indonesia. Hence, the production of anti-GAD65 Abs reagent in Indonesia may reduce the cost and improve the quality of diabetes care in Indonesia. We produce reagent to detect anti-GAD65-Abs using bovine brain tissue as source of GAD enzyme in 3 steps. Step 1, isolation, purification of GAD65 from bovine brain tissue and used it as a primary antigen to stimulate the generation of anti-GAD65 antibodies in Wistar rat. Step 2, the purified GAD65 antibodies were than used as a secondary antibody to induce the production of anti-anti-GAD65-antibodies in Wistar rat and rabbit. Step 3. Labeling  anti-anti GAD65-antibodies with alkaline phoshpatase and peroxidase, and detecting anti-GAD65Abs previously detected using commercial kit. The anti-anti-GAD65- antibodies reagent produced in our laboratories  successfully identify anti-GAD65-Abs of type 1 diabetic patients previously detected  with commercial reagent. (Med J Indones 2005; 14: 197-203)Keywords: GAD, type-1 Diabetes

High parity and hormonal contraception use as risk factors for cervical cancer in East Kalimantan

Medical Journal of Indonesia Vol 19, No 4 (2010): November
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (32.845 KB)

Abstract

Aim: To find risk factors associated with cervical cancer.Methods: This a case-control study conducted in A.W. Sjahranie County General Hospital at Samarinda East Kalimantan from January until July 2009. There were 58 patients for each case and control group. Variables in this study were age, menarche, menopause, age of first marriage, parity, spouse’s smoking status, hormonal contraception use, type of hormonal contraception, duration of hormonal contraception, IUD (intra uterine device) contraception use and duration of IUD contraception.Results: final data analysis shows that parity and duration of hormonal contraception use increased the risk of cervical cancer. Women who had 5-12 children than 0-4 children had 2.6-folds increased risk to be cervical cancer. Compared to women never use of hormonal contraception, those who ever had hormonal contraception for 1-4 years and 5-25 years had two time and 4.5 times increased risk to be cervical cancer respectively.Conclusion: Cervical cancer screening recommended to be focused on high-risk groups, among others, women with the number of children born more than fi ve people or women in particular users of hormonal contraception methods with a range of use more than fi ve years. (Med J Indones 2010; 19:268-72)Keywords: Cervical cancer, hormonal contraception, menarche, parity

EMSA Eritin Blocks Nuclear Factor-Kappa B in Diabetes Mellitus Mice Model

The Journal of Experimental Life Science Vol 5, No 1 (2015)
Publisher : Graduate School, University of Brawijaya

Show Abstract | Original Source | Check in Google Scholar

Abstract

Diabetes mellitus is one of the common metabolic disorders with increasing prevalence during recent years with hyperglycemia as its characteristic. DM has been shown to be a state of free radicals over production resulted from hyperglycemia that can activate cellular signaling pathways transcription of factor NF-κB which stimulates the production of several inflammatory mediators andl lead to chronic inflammation. Chronic inflammation is implicated in β-cell damage and function and promotes apoptosis. EMSA Eritin is a polyherbal consisting of soy bean extracts, coconut water extract and red rice extract that assumed to be antidiabetic and anti-inflammatory. This study will assess the effectiveness of EMSA Eritin against inflammation in diabetes mellitus by measuring levels of NF-κB produced by immunocompetent cells in DM mice model. Streptozotocin 100 mg.kg-1 BW is used to induce diabetes mellitus in mice. Oral administration of EMSA Eritin was given for 14 days with dose of 0.3125 mg.g-1 BW, 3.125 mg.g-1 BW and 31.25 mg.g-1 BW. Data were analyzed using One-way ANOVA (p<0.05) and Duncan test using SPSS 16.0 for Windows. The results showed that EMSA Eritin can be used as an alternative therapy for the treatment of DM. The level of NF-κB in diabetic mice significantly decreased when the mice received EMSA Eritin.Keywords: Diabetes Mellitus, EMSA Eritin, NF-κB, ROS

Bone microstructure and atomic periodic disharmonization in osteoporosis

Universa Medicina Vol 31, No 2 (2012)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Original Source | Check in Google Scholar

Abstract

BackgroundBoth cortical and cancellous bone display a complex, porous microstructure whose properties depend on the macrostructure of bone as well as age and health of the individual. The aim of this study was to compare the microstructure and characteristics of mineral atoms in osteoporotic and normal bone. MethodsA prospective laboratory experimental study was conducted from August to December 2010 at several hospitals in Banjarmasin. Twenty patients with osteoporosis and twenty six normal patients were involved in this study. Bone obtained from surgery was analyzed for microstructure by scanning electron microscopy (SEM), while mapping of mineral atoms was performed by means of SEM-Energy dispersive X-ray spectroscopy (SEM-EDAX) at the Brawijaya University, Malang.     ResultsThe osteoporotic subjects with mean age of 64.65 ± 16.41 years were older than the normal bone subjects with mean age of 39.38 ± 17.16 years. The body mass index was similar in both groups of subjects. From SEM-EDAX results, three patterns of mineral atoms were apparent in osteoporotic and normal bone. In osteoporotic bone, these patterns were indicative of a disordered substitution or incorporation process. SEM results showed degeneration of microarchitecture (resorption cavities, perforations, and prominent granules) in osteoporotic but not in normal bone. There was a significant difference in microstructure between osteoporotic and normal bone, which was caused by differences in atomic properties.    ConclusionsMicrostructural abnormalities of bone and disharmonization of mineral atoms in the periodic system were found in osteoporosis.

Computational Study of Oxidized Linoleic Acid on Dipeptidyl Peptidase-4 Shedding

Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
Publisher : Proceeding International Conference on Global Resource Conservation

Show Abstract | Original Source | Check in Google Scholar

Abstract

Dipeptidyl Peptidase-4 is a epithelial transmembrane protein of many cell type such as gastrointestinal tract, biliary tract, pancreas, kidney, thymus, uterus, prostate and capillary endothelial cell. It has a cytoplasmic domain (amino acids 1-6), transmembrane domain  (amino acid 7-28) and extracellular domain (amino acids 29-766). Dipeptidyl Peptidase-4 also found in soluble form of serum and other body fluid. Soluble DPP-4 has not a cytoplasmic domain and transmembrane domain. The level of soluble dipeptidyl peptidase-4 increased in diabetes. In diabetes, Hydrogen Peroxide as Reactive Oxygen Species (ROS) involved in transmembrane DPP-4 shedding to produce soluble DPP-4. Hydrogen peroxide can be converted into hydroxyl radicals (•OH) which are highly reactive. Hydroxyl radicals can oxidize  fatty acid such as linoleic acid which is found mainly in Low Density Lipoprotein (LDL). This study was aimed to elucidate the molecular mechanism of oxidation to shed  transmembrane DPP-4 to produce soluble DPP-4. The DPP-4 model used is DPP-4 with 10-766 amino acid derived from Uniprot and 3D Jigsaw program. Oxidized linoleic acids were derived from NCBI pubchem. DPP-4 as a receptor was docked with oxidized linoleic acids as ligand by patchdock program. And the result show that 13-HODE more easily interact acid with DPP-4than  the other oxidized linoleic. That  oxidants had the active site on DPP-4 (Threonine 36 and Alanine 37) that allow the shedding of transmembrane DPP-4 to produce soluble DPP-4. But that oxidant also interact with the other  site  of DPP-4 that may destroy the catalytic domain.Keywords: DPP-4; oxidized linoleic acid; shedding

PROSPEK SARI BUAH TIN LOKAL (Ficus glumerata Rob) SEBAGAI AGEN PRESERVASI MOTILITAS SPERMATOZOA KAMBING

Jurnal Kedokteran Hewan Vol 7, No 1 (2013): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (358.473 KB)

Abstract

Penelitian ini bertujuan mengetahui prospek sari buah tin lokal (Ficus glumerata Rob) di dalam pengencer berbasis Tris dan kuning telur untuk mempertahankan motilitas progresif spermatozoa kambing peranakan Boer yang disimpan pada suhu 4-5 C. Perlakuan yang diberikan adalah perbedaan dosis sari buah tin dalam masing-masing pengencer yakni 0, 1, 2, 3, 4, 5, 6, dan 7% (S0, S1, S2, S3, S4, S5, S6, dan S7). Pengamatan motilitas progresif spermatozoa dilakukan segera setelah pengenceran dan setiap 24 jam sampai jam ke-144 setelah pengeceran dan penyimpanan. Persentase motilitas progresifs permatozoa pada kelompok S4 (39,0±2,33) dan S6 (38,0±2,49) lebih tinggi dibandingkan S0; S1; S2; S3; dan S5 yakni masing-masing 32,0±1,86; 30,0±2,11; 29,0±1,80; 31,5±3,66; dan 34,5±3,02. Persentase motilitas terendah ditunjukkan oleh S7 yaitu 22,0±3,59. Dari hasil penelitian ini dapat disimpulkan bahwa sari buah tin mampu mempertahankan motilitas progresif spermatozoa kambing dan konsentrasi optimal dicapai pada 4 dan 6%.

INTERVENSI CYTOADHERENCE SEBAGAI PELUANG UNTUK PENCEGAHAN DAN TERAPI MALARIA BERAT

Jurnal Kedokteran Brawijaya Vol 22, No 2 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Original Source | Check in Google Scholar

Abstract

Abstract  In  malarial  infection,  erythrocytes  infected  wih  Plasmodium  falciparum  bind  to  endothelial  vascular  (endothelial cytoadherence). This binding is implicated in the forming of sequestre and  rosette that affects the vascular circulation, and thus injures  the capillary wall. This mechanism is important in pathogenesis of malarial due to dysfunction of several organs. There are several receptors of  cytoadherence in human e.g Thrombospondine (TSP), CD-36, ICAM-1, and ELAM-1 as well as specific ligands of the parasite  e.g  Plasmodium falciparum Erythrocyte Membrane Protein-1 (PfEMP-1), 220 kDa protein of Pf60, Pf332, sequestrin, Pfaldhesin and STEVOR.  PfEMP-1 has been revealed as a molecule that is responsible for pathogenesis of severe malaria. This protein can pass parasitophorous vacoular membrane (PVM) of the parasite by attaching its molecule to carry Protein Export Elemen (PEXEL) and than go to the surface of erythrocytes in combination with specific helper protein in maeurer cleft.  The intervention on cytoadherence process through blocking of specific ligand directly or blocking the translocation of this ligand to the surface of erythrocytes might be important in regulating the outcome of malarial infection.  Key words : Cytoadherence, severe malaria, intervention, adhesion molecule.

INHIBIN B MENURUNKAN KONSENTRASI FOLLICLE STIMULATING HORMONE (FSH) PADA TIKUS PUTIH (Rattus norvegicus): UPAYA PENGEMBANGAN KONTRASEPSI HORMON PRIA BERBASIS PEPTIDA

Jurnal Kedokteran Hewan Vol 9, No 1 (2015): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (213.745 KB)

Abstract

Penelitian ini bertujuan mengetahui efek injeksi inhibin B terhadap penurunan konsentrasi follicle stimulating hormone (FSH) di dalamserum pada tikus putih (Rattus norvegicus). Dalam penelitian ini digunakan 24 ekor tikus putih berjenis kelamin jantan dengan strain Wistar berumur 4 bulan dengan bobot badan 150-200 g. Tikus-tikus dikelompokkan secara acak ke dalam 4 kelompok, yaitu KK0, KP1, KP2, dan KP3, masing-masing kelompok terdiri atas 6 ekor. Kelompok KK0 merupakan kelompok kontrol hanya diinjeksi dengan phosphate buffer saline (PBS), sedangkan kelompok KP1, KP2, dan KP3 diinjeksi dengan inhibin B dengan dosis berturut-turut 25, 50, dan 100 pg/ekor. Injeksi inhibin B dilakukan secara intraperitoneum sebanyak 5 kali selama 48 hari dengan interval waktu 12 hari. Injeksi pertama inhibin B dilarutkan dengan0,05 ml PBS dan 0,05 ml Freud’s complete adjuvant (FCA). Injeksi kedua sampai kelima, inhibin B dilarutkan dengan 0,05 ml PBS dan 0,05 ml Freud’s incomplete adjuvant (FICA). Pada hari ke-6 setelah injeksi inhibin B terakhir, tikus dikorbankan secara dislocatio cervicalis,lalu darah dikoleksi langsung dari jantung dan didiamkan hingga didapatkan serum untuk pemeriksaan konsentrasi FSH dengan menggunakan metode enzyme-linked immunosorbent assay (ELISA). Hasil penelitian menunjukkan bahwa injeksi inhibin B dengan dosis 100 pg/ekor menurunkan konsentrasi FSH secara nyata (P<0,05) bila dibandingkan dengan kelompok kontrol. Berdasarkan hal tersebut, inhibin B berpeluang untuk dikembangkan sebagai kandidat kontrasepsi pria hormon berbasis peptida.