Articles

Found 34 Documents
Search

Different Percentages of Gonad Maturity on The Fertility and Haching Rate in Artificial Breeding of Abalone (Haliotis asinina)

SAINTEK PERIKANAN Vol 6, No 1 (2010): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

Show Abstract | Original Source | Check in Google Scholar | Full PDF (236.656 KB)

Abstract

Abalone is one of the marine mollusk that has high economic value. Mass abalone cultivation requires the mature gonads broodstock to produce seeds, but the maturity of the gonads of male and female often can not be simultaneously. Artificial insemination using a solution of ammonia is one solution to produce abalone seed in the hatchery. Ammonia solution can increase motility, fertilization rate of eggs and abalone seed production, so the provision of seeds for cultivation of abalone is independent from nature. The research of artificial insemination was carried out at various levels of gonadal maturation dams abalone (H. asinina) to determine the percentage of fertility and hatching rate of eggs from each Gonad Maturity Level. In stadia gonadal recovery, no fertilization occurs, whereas in the gonads maturing stadia had fertilization and hatching rate 24.33 ± 2.08% and 22.12 ± 2.18%, respectively at ripe stadia, gonads had been fertilization and hatching rate were 94.67% ± 1.53% and 82.82% ± 3.58%, wasn’t recorded in the stadia spent fertilization did not occur because the eggs were released in culture media. Gonad Maturity Level, thus can be used in artificial insemination was the ripe gonadal stadia, which had the highest percentage of both levels of fertilization and hatching rate with a diameter ranging from 189.6-252.8 μm.   Key Words : Abalone (H. asinine); Artificial breeding; Gonad Maturity Level; Hatching Rate  

The Addition of Ammonia Solution with Different Concentrations in the Sperms on the Percentage Ovulation Egg of Abalone, Haliotis asinina

SAINTEK PERIKANAN Vol 5, No 1 (2009): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

Show Abstract | Original Source | Check in Google Scholar | Full PDF (156.172 KB)

Abstract

The aim of this research was to know the impact of addition of ammoniak in different concentration in the sperm on the percentage of egg ovulation. The research method was carried out by completely randomized design with four treatments and three replicates, respectively. Those treatments were the addition of ammoniac solution with concentration of 0% (treatment A), 0.005% (treatment B), 0.010% (treatment C), and 0.015% (treatment D). The data was analyzed by using ANOVA and Duncan’s Multiple Range Test to know the effect of between the other treatments. The results showed that was the highly significant difference effect (p<0.01) on the egg ovulation of Abalone (H. asinina). The highest egg ovulation rate was obtained in treatment B (94.67% ). The ovulation rate was significantly higher than those in treatments C, D, and A, respectively. According to the results, the best concentration of the ammonia solution to the abalone sperm added was 0.005%. Furthermore, the regression test also showed a positive, linear relationship between spermatozoa motility rate and egg ovulation rate. However, the analysis of orthogonal polymonial provided a ammonia solution with the highest ovulation rate of 0.0057%.  Keywords: Concentration, ammonia solution, ovulation egg, abalone, H. asinina

PERFORMA PERTUMBUHAN HARPATICOIDA Tigriopus sp. DALAM KONDISI KULTUR SEMI MASSAL DENGAN DIET MIKROALGA YANG BERBEDA

Sains Akuakultur Tropis Vol 2, No 2 (2018)
Publisher : Departemen Akuakultur FPIK UNDIP

Show Abstract | Original Source | Check in Google Scholar

Abstract

Tigriopus sp. is a copepod included harpacticoid ordo which is a live food organism for marine fish and shrimp larvae. The lack of information about the appropriate microalgae diet is an obstacle in the semi-mass culture of Tigriopus sp. The purpose of this study was to determine the effect of different microalgae diets and the type of microalgae diet which provides the best growth performance of Tigriopus sp. in semi-mass culture conditions. The experimental design used was a Completely Randomized Design (CRD) with 4 treatments and 4 repetitions namely Tigriopus sp. with a microalgae diet; A. Chlorella vulgaris; B. Tetraselmis chuii; C. Chaetoceros calcitrans; and D. Isochrysis galbana. The results showed that giving C. calcitrans microalgae diet has significant effect (p<0.05) and provides growth performance of Tigriopus sp. Best total density reached 9,75±0,50 inds.mL-1; specific growth rate of the population was 0,114±0,003 inds.day-1 and egg production was 9.26 ± 0.09 eggs.ind.-1 Keywords: Tigriopus sp., growth performance, semi-mass culture, microalgae diet

PENGGUNAAN JENIS MEDIA KULTUR TEKNIS TERHADAP PRODUKSI DAN KANDUNGAN NUTRISI SEL Spirulina platensis

SAINTEK PERIKANAN Vol 4, No 2 (2009): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

Show Abstract | Original Source | Check in Google Scholar | Full PDF (168.332 KB)

Abstract

ABSTRAKPenelitian ini bertujuan untuk mengetahui perbedaaan tiga jenis komposisi media kultur teknis terhadap hasil produksi dan kandungan nutrisi sel S. platensis dan untuk mengetahui jenis media yang terbaik dari ketiga jenis komposisi tersebut. Kultur sel S. platensis dilakukan dengan menggunakan bak fiber gelas volume 300 l, dengan periode kultur selama 7 hari dengan kepadatan awal 50.000 sin/ml. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 3 perlakuan dan masing-masing terdiri dari 3 ulangan. Kemudian dari masing-masing kultur tersebut dianalisis kandungan nutrisinya. Hasil penelitian menunjukkan bahwa perlakuan W berbeda sangat nyata (p<0.01) terhadap kelimpahan sel S. platensis pada puncak pertumbuhan dan kandungan nutrisi (protein, lemak, karbohidrat dan abu). Kelimpahan sel, kandungan protein dan lemak tertinggi terjadi pada perlakuan W yang masing-masing nilainya 5,684 log sin/ml, 67,58%, dan 11,61%. Disimpulkan bahwa media Walne merupakan media yang terbaik pengaruhnya terhadap kelimpahan sel pada puncak populasi dan nilai nutrisi, khususnya nilai kandungan protein dan lemak.Kata kunci : S. platensis, Media kultur, Produksi sel, Kandungan nutrisi.ABSTRACTThe purpose of this research was to know the differences three technical culture media composition and the best compotition of technical media culture on the production and nutrition contents of S. platensis cells. The material of S. platensis cells were cultured by using fiber glass tank volume 300 l. Those cells were cultured during 7 days with initial cell density of 50.000 sin/ml. The experimental method was employed in this research with completely randomized design, through three treatments and three replicates, respectively. Then each treatment was analyzed for cells production and it’s nutritional content. The research results showed that W treatment was highly significantly different effect (p<0.01) on the maximal cell densities and nutrition content (protein, fat, and ash) of S. platensis cells. The highest cell density and protein content were W treatment of 5,684 log sin/ml and 67,58%, respectively. There was concluded that Walne media had significant effects on the best cell density and nutrition value of S. platensis cells, especially in the protein and fat contents.Key words : S. platensis, Culture medium, Cells production, and Nutrition content

PERTUMBUHAN BAKTERI PROBIOTIK Alkaligenus sp. DAN Flavobacterium sp. YANG DIISOLASI DARI USUS UDANG PADA MEDIA KULTUR MOLASE DAN KAOLIN

SAINTEK PERIKANAN Vol 4, No 1 (2008): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

Show Abstract | Original Source | Check in Google Scholar | Full PDF (277.578 KB)

Abstract

Penelitian ini bertujuan untuk mengetahui konsentrasi tetes tebu (molase) dan tepung kaolin yang tepat untuk media kultur massal bakteri Alkaligenes sp. dan Flavobacterium sp. pada kondisi lingkungan (suhu, salinitas, dan pH) yang terbaik bagi pertumbuhan. Metoda yang digunakan pada penelitian ini adalah eksperimen dengan   3 (tiga) macam perlakuan dan masing-masing dilakukan pengulangan sebanyak 3 (tiga) kali. Perlakuan perbedaan  konsentrasi  molase yaitu M1 (16 gr/mL), M2 (32 gr/mL), dan  M3 (48gr/mL). Sedangkan perlakuan perbedaan konsentrasi tepung kaolin adalah K1 (8 gr/mL), K2 (16 gr/mL), dan K2 (32 gr/mL). Data yang dikumpulkan dalam penelitian ini adalah kelimpahan bakteri yang diukur melalui Opical Density pada spectrofotometer.  Analisa statistik uji varian, uji wilayah  ganda duncan telah digunakan dalam analisa data penelitian ini untuk mengetahui pengaruh perbedaan berbagai kondisi lingkungan media kultur. Hasil penelitian menunjukkan bahwa perlakuan perbedaan konsentrasi molase berpengaruh sangat nyata (p<0,01) terhadap pertumbuhan bakteri Alkaligenes sp. dan Flavobacterium sp. Pada perlakuan perbedaan konsentrasi tepung kaolin menunjukkan adanya pengaruh yang sangat nyata (P<0,01) terhadap pertumbuhan bakteri Alkaligenes sp., tetapi tidak berpengaruh nyata terhadap pertumbuhan bakteri Flavobacterium sp.   Kata Kunci : Bakteri probiotik, pertumbuhan, jenis media, kondisi lingkungan

PENGGUNAAN MEDIA KULTUR YANG BERBEDA TERHADAP PERTUMBUHAN Chlorella sp

SAINTEK PERIKANAN Vol 4, No 1 (2008): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

Show Abstract | Original Source | Check in Google Scholar | Full PDF (233.456 KB)

Abstract

Usaha budidaya ikan di perairan payau dan laut semakin berkembang dan tidak dapat terlepas dari tahap pembenihan. Diperlukan budidaya benih dengan pemberian pakan alami yang cukup dan berkualitas. Pakan alami belum dapat digantikan oleh pakan buatan terutama pada saat awal pemeliharaan larva. Salah satu jenis pakan alami adalah Chlorella sp. yang diberikan kepada jenis zooplankton maupun langsung kepada kultivan ikan atau udang. Media kultur Walne, Gullard’s f/2 dan Erdschreiber merupakan media yang cocok digunakan untuk mengkultur phytoplankton. Tujuan penelitian adalah mengetahui pengaruh penggunaan media Walne, Gullard’s f/2 dan media Erdschreiber terhadap pertumbuhan Chlorella sp dan mengetahui media terbaik dari ketiga media tersebut terhadap pertumbuhan Chorella sp. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap dengan 3 perlakuan dan 3 kali ulangan, yaitu perlakuan A menggunakan Media Walne, perlakuan B menggunakan Media Guillard’s f/2, dan perlakuan C menggunakan Media Erdschreiber. Hasil penelitian menunjukkan bahwa penggunaan media kultur yang berbeda berpengaruh nyata (P<0,05) terhadap waktu lag phase dan berpengaruh sangat nyata (P<0,01) terhadap konstanta pertumbuhan spesifik, puncak populasi dan kepadatan akhir Chlorella sp. Media Guillard f/2 memberikan pertumbuhan Chlorella sp terbaik dengan waktu lag phase tercepat (0,290 hari), konstanta pertumbuhan spesifik terbaik (0,655), puncak populasi tertinggi (8,53 x 107  sel/ml) dan kepadatan akhir tertinggi (2,77 x 106 sel/ml)   Kata Kunci : media, kultur, pertumbuhan, Chlorella sp

Using the Live Food Enrichment with the Squid Egg Extract on the Quantity and Quality of Rotifer, Brachionus plicatilis O.F. Muller

SAINTEK PERIKANAN Vol 3, No 2 (2008): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

Show Abstract | Original Source | Check in Google Scholar | Full PDF (164.167 KB)

Abstract

The Objective of the research was to know the effect of live food enrichment of rotifer with squid egg extract on the growth patterns and quality of rotifer, especially the EPA and DHA contents. The experiment method was employed in this research and it carried out in the aquaculture laboratory, Faculty of Fisheries and Marine Sciences, Diponegoro University. The research planning was a completely randomized design with five treatments and three replicates, respectively. The recording data was analyzed to get the variables of rotifer growth patterns and the most of rotifer samples were analyzed EPA and DHA contents in the chemical and biochemical laboratory center, Gajah Mada University. The results showed that the addition of squid egg extract in the culture media of rotifer was highly significant effect (p<0.01) on the specific growth rate, the maximal density, and the final density of rotifer culture. However, there was also highly significsnt effect (p<0.01) on the nutrition quality through EPA and DHA contents. To increase the quantity and quality of rotifer, B. plicatilis O.F. Muller in the culture was addition of squid egg extract of 1,2 g/1 million ind. Rotifers during twelve two hours in the rotifer culture. Key word : Rotifer, Brachionus plicatilis O.F. Muller, enrichment, quantity and quality, EPA dan DHA

Effect of Bakers Yeast, Vitamin B12, and Vitamin C as Nutritional Improvement of Food on the Density Production of Brachionus plicatilis

SAINTEK PERIKANAN Vol 5, No 2 (2010): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

Show Abstract | Original Source | Check in Google Scholar | Full PDF (231.9 KB)

Abstract

The important factor of seedling production is live food with good quality. One way to improve quqlity of live food, Brachionus plicatilis, to gain a high quality of larvae in seedling production, is through implementing food enrichment. Chlorella sp, which is its live food, can be enriched by combination of baker’s yeast, vitamin B12 and vitamin C, which then given, in order to increase Branchionus plicatilis growth and nutrition content. Purpose of this study is to acknowledge comparative population growth and improvement of Brachionus plicatilis with different kinds of foods enrichment, to understand the right food enrichment composition for Brachionus plicatilis growth. This research are laboratory experimental, with 10 ind/ml early spread density on 5 litres capacity container. The experiment using a Completely Randomize Design with 4 treatment and 3 repetitions. That four treatments included A (Chlorella sp), B (Chlorella sp + baker’s yeast), C (Chlorella sp + baker’s yeast + vitamin B12), D (Chlorella sp + baker’s yeast + vitamin B12 + vitamin C). Result from the research shows that implementation of food enrichment, which using baker’s yeast, vitamin B12 and vitamin C, brings about a great significant differences (p<0,01) in each treatment to population growth. D is the best treatment with specific growth rate of 0,661, maximal density of 530,4 ind/ml and final density of 381,7 ind/ml. Key Words: Elements of Food Enrichment, Population, Brachionus plicatilis

Different Percentages of Gonad Maturity on the Fertility and Haching Rate in Artificial Breeding of Abalone (Haliotis Asinina)

SAINTEK PERIKANAN Vol 5, No 2 (2010): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

Show Abstract | Original Source | Check in Google Scholar | Full PDF (229.882 KB)

Abstract

Abalone is one of the marine mollusk that has high economic value. Mass abalone cultivation requires the mature gonads broodstock to produce seeds, but the maturity of the gonads of male and female often can not be simultaneously. Artificial insemination using a solution of ammonia is one solution to produce abalone seed in the hatchery. Ammonia solution can increase motility, fertilization rate of eggs and abalone seed production, so the provision of seeds for cultivation of abalone is independent from nature. The research of artificial insemination was carried out at various levels of gonadal maturation dams abalone (H. asinina) to determine the percentage of fertility and hatching rate of eggs from each Gonad Maturity Level. In stadia gonadal recovery, no fertilization occurs, whereas in the gonads maturing stadia had fertilization and hatching rate 24.33 ± 2.08% and 22.12 ± 2.18%, respectively at ripe stadia, gonads had been fertilization and hatching rate were 94.67% ± 1.53% and 82.82% ± 3.58%, wasn’t recorded in the stadia spent fertilization did not occur because the eggs were released in culture media. Gonad Maturity Level, thus can be used in artificial insemination was the ripe gonadal stadia, which had the highest percentage of both levels of fertilization and hatching rate with a diameter ranging from 189.6-252.8 μm. Key Words: Abalone (H. asinine); Artificial breeding; Gonad Maturity Level; Hatching Rate

KAJIAN STANDAR PRODUK PORSELEN UNTUK PERALATAN MAKAN DAN PERALATAN DAPUR

JURNAL STANDARDISASI Vol 14, No 2 (2012): Vol. 14(2) 2012
Publisher : Badan Standardisasi Nasional

Show Abstract | Original Source | Check in Google Scholar

Abstract

Porselen adalah bahan keramik yang dibuat dengan memanaskan bahan baku, pada umumnya termasuk tanah liat dalam bentuk kaolin, dalam tanur pada suhu antara 1.200°C (2192°F) dan 1.400°C (2552°F). Berdasarkan data Badan Pusat Statistik tahun 2010, nilai ekspor produk porselen untuk peralatan makan dan peralatan dapur dari Indonesia ke 5 Negara tujuan ekspor (Amerika, Inggris, Jepang, Belgia, Korea) mencapai US$ 59.741.798. Dalam era perdagangan bebas, semua negara dapat dipertimbangkan sebagai pasar yang penting bagi eksportir suatu negara, termasuk Indonesia. Strategi Indonesia ke depan adalah mendorong ekonomi nasional dengan meningkatkan volume ekspor dan menghambat arus impor produk akhir yang sub standar. Dua hal tersebut harus didukung dengan standar nasional yang berkemampuan memenuhi aspek persyaratan teknis, regulasi dan batasan lainnya yang dipersyaratkan oleh negara tujuan ekspor. Selain itu, standar juga harus berkemampuan menguatkan persyaratan teknis K3L untuk mengeliminasi arus impor. Tujuan kajian ini adalah mengetahui jumlah SNI dari produk porselen untuk peralatan makan dan peralatan dapur, memberikan rekomendasi pengembangan terkait persyaratan teknis dalam SNI. Metode yang digunakan dalam kajian ini adalah metode deskriptif yaitu menjelaskan data – data mengenai standar dan regulasi yang didapatkan melalui penelusuran pustaka. Kajian ini telah mengidentifikasi standar terkait produk porselen untuk peralatan makan dan peralatan dapur yaitu 2 standar SNI, 3 standar ISO, 1 standar ASTM, 2 standar JIS, 6 standar BS EN. SNI 12-4682:1998 - Alat masak keramik (cooking ware) telah berusia lebih dari 5 tahun serta persyaratan kandungan timbal dan cadmium tidak harmonis dengan standar ISO, sehingga perlu dikaji ulang dengan menggunakan referensi ISO 6486:1999 Ceramic ware, glass-ceramic ware and glass dinnerware in contact with food -- Release of lead and cadmium -- Part 2: Permissible limits, dan memperhatikan juga kemampuan industri nasional.