SONY HERU SUMARSONO
School of Life Sciences and Technology, Bandung Institute of Technology, Jalan Ganeca 10, Bandung 40132

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Development of Domestic Cat Embryo Produced by Preserved Sperms

HAYATI Journal of Biosciences Vol 15, No 4 (2008): December 2008
Publisher : Bogor Agricultural University, Indonesia

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Abstract

The ability to mature and fertilize oocytes of endangered species may allow us to sustain genetic and global biodiversity. Epididymis sperms may be the last chance to ensure preservation of genetic materials after injury or death of a valuable animal. Studies have been conducted to determine wether both epididymis sperms and oocytes can be used to produce viable embryos and offspring. The purpose of this study was to determine how long cats sperms contained in epididymis were remain motile and had intact membranes when preserved at 4 oC, and to determine whether such those preserved sperms are able to fertilize oocytes. Epididymis was preserved immediately in phosphate buffer saline at 4 oC for 1, 3, and 6 days. The observation of sperm quality and viability after preservation was performed by vital staining acrosom and Hoechst-Propidium Iodine. Biological functions of sperms were evaluated by in vitro culture technique for fertilization, micro fertilization and embryonic development rate in CR1aa medium. The results showed that average motility of sperms collected from ductus deferens, cauda and corpus epididymis decreased not significantly (P > 0.05) from 0, 1, 3, and 6 days of preservation times (from 83.0%, 80.2%, 79.0%; 80.9%, 75.0%, 75.5%; 52.0%, 63.2%, 55.0% to 34.6%, 34.6%, 33.3%, respectively). The general results showed that sperms from epididymis preserved for 1, 3, and 6 days can be used for IVF. The rate of embryonal cleavage produced by IVF technique using sperms collected from epididymis preserved for 1-, 3- and 6-days were 33.3, 26.7, and 20.0%, respectively and significantly different (P < 0.05) from that of controll (50.0%). In conclusion, sperms contained in epididyimis preserved at 4 oC in PBS (Phospate Buffer Saline) for 1-6 days can be used to IVF and in vitro production of cat embryos. Key words: gamet, preservation, in vitro fertilization

Hubungan Antara Reactive Oxygen Species (ROS), Superoxide Dismutase (SOD) dengan Protein α-Sinuklein-larut Air pada Batang Otak Tikus yang Diinduksi Rotenon

Global Medical & Health Communication (GMHC) Vol 3, No 2 (2015)
Publisher : Fakultas Kedokteran Universitas Islam Bandung

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Abstract

Abstrak Parkinson adalah salah satu penyakit neurodegeneratif dengan ganggunan gerak bila kematian neuron dopaminergik lebih dari 70 %. Paparan neurotoksin diduga menjadi penyebab terjadinya Parkinson sporadik. Tujuan penelitian ini adalah menentukan kondisi stres oksidatif pada batang otak tikus Wistar yang diinduksi rotenon. Metode: Penelitian eksperimental dengan menggunakan tikus Wistar jantan. Variabel bebas: kelompok tikus, lama perlakuan, waktu pengamatan, dan lokasi di batang otak. Variabel terikat: konsentari SOD, konsentrasi protein α-sinuklein-larut air, dan densitas ROS. Densitas ROS berbeda secara signifikan antara kelompok perlakuan (sig: 0,029), waktu pengamatan (sig: 0,0001), dan lokasi di batang otak (sig: 0,001). Konsentrasi SOD tidak berbeda secara signifikan antar kelompok perlakuan (sig:0,566), waktu pengamatan (sig:0,441) dan lokasi di batang otak (sig: 0,091). Konsentrasi protein α-sinuklein-larut air berbeda secara signifikan antar kelompok perlakuan (sig: 0,001), waktu pengamatan (sig: 0,001) tetapi tidak berbeda secara signifikan pada lokasi di batang otak (0,625). Densitas ROS relatif tertinggi pada hari ke-10 dan 40. Sementara itu konsentrasi SOD pada hari ke-10 dan 40 relatif rendah sedangkan konsentrasi protein α-sinuklein-larut air pada hari ke-10 relatif tinggi dibandingkan dengan hari ke-40. Dengan demikian dapat disimpulkan bahwa stres oksidatif pada batang otak tikus Wistar yang diinduksi rotenon berpengaruh pada struktur protein α-sinuklein.   Kata kunci: Batang otak, protein α-sinuklein, ROS, rotenon, SOD, stres oksidatif   Abstract Parkinson is the neurodegenerative disease with movement disordered, if the dopaminergic neurons dead more than 70%. Neurotoxins exposure is predicted cause sporadic Parkinson. The research aim is to determine oxidative stress stage in the brainstems Wistar rat’s treated-rotenone. Methods: An experimental study using male Wistar rats. The independent variable: groups of rats, long treatment, observation time, and location in the brainstem. The dependent variable: SOD concentration, concentration of protein α-synuclein-water soluble, and ROS density. ROS density significantly different among treatment groups (sig: 0,029), observation time (sig: 0.0001), and the location in the brainstem (sig: 0,001). SOD concentrations not significantly different among treatment groups (sig: 0.566), observation time (sig: 0.441) and the location in the brainstem (sig: 0.091). The concentration of protein α-synuclein-water soluble significantly different among treatment groups (sig: 0,001), observation time (sig: 0,001), but not significantly different at the location in the brainstem (0,625). ROS density relatively high at day 10 and 40. Meanwhile SOD concentration on day 10 and 40 are relatively low. And the concentration of α-synuclein protein-water soluble on day 10 is relatively higher than on day 40. The conclution is oxidative stress in the brainstem Wistar rat’s treated-rotenone effected on the protein α-synuclein structure Key word: α-synuclein protein, brainstem, oxidative stress, ROS, rotenone, SOD

Face Shape Variation Among Sundanese People from Western Java, Indonesia

HAYATI Journal of Biosciences Vol 22, No 1 (2015): January 2015
Publisher : Bogor Agricultural University, Indonesia

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Abstract

The face is an important visual stimulus in daily life and each face identifies a particular person. The bone structure of the skull along with various soft tissues and coloration influence perception of the face. Facial averageness, and bilateral symmetry are the two most commonly used criterion of facial attractiveness, yet, both may be perceived differently based on hormonal status of the person observed. Facial perceptions may also differ according to cultural norms. In this research, we examined variations in face-shape among Sundanese male and female adults aged 18 to 40. We applied geometric-morphometric methods to analyze the landmark-based morphological variations in the frontal and lateral views of subjects’ faces. We identified five types of female frontal face views and four of male. We also identified five types each of female and male lateral face views. The trichion, gonion and gnathion were three most variable landmarks among the face views in our study, and highly determined the shape of the individuals’ faces. Multiple face type variation may refer to many categories of attractive faces since there is no exactly perfect category in the assessment of facial attractiveness by the viewers. Therefore, we believe that the configuration of facial features cannot constitute the sole visual criterion of facial attractiveness.

PRODUKSI EMBRIO KUCING SECARA IN VITRO DARI SPERMATOZOA HASIL PRESERVASI MELALUI FERTILISASI MIKRO

Jurnal Kedokteran Hewan Vol 7, No 1 (2013): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

Penelitian ini bertujuan mengetahui motilitas dan kemampuan memfertilisasi sperma kucing yang berasal dari epididimis yang disimpan pada suhu 4° C. Epididimis disimpan dalam media phosphate buffer saline (PBS) pada suhu 4 C selama 1, 3, dan 6 hari. Viabilitas spermatozoa diamati dengan pewarnaan hoechst-propidium iodine. Fungsi biologis spermatozoa dievaluasi melalui teknik kultur in vitro dengan fertilisasi mikro dan perkembangan embrio di dalam media kultur CR1aa. Hasil penelitian menunjukkan persentase spermatozoa hidup pada hari ke-1, 3, dan 6 penyimpanan masing-masing adalah 81,0; 71,7; dan 70,7% (duktus deferens), 84,0; 81,2; dan 63,2% (kauda epididimis), 84,0%; 75,0; dan 74,7% (korpus epididimis). Persentase pronukleus (PN) yang didapat dengan teknik intra cytoplasmic sperm injection (ICSI) menggunakan spermatozoa epididimis pada hari ke-1, 3, dan 6 hari penyimpanan masing-masing adalah 8,0; 10,0; dan 5,9%. Preservasi epididimis pada suhu 4 C dalam PBS dapat digunakan untuk fertilisasi dan menghasilkan embrio kucing secara in vitro.

Ekstrak Etanol Biji Pinang Muda (Areca catechu L) Meningkatkan Apoptosis pada Sel Testikular Mencit (Mus musculus L)

Journal of Biota Biota Volume 3,Nomer 1 Tahun 2018
Publisher : University of Atma Jaya Yogyakarta

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Abstract

Biji pinang memiliki potensi sebagai bahan antifertilitas dan diduga dapat menginduksi apoptosis sel-sel spermatogenik. Penelitian ini membahas tentang perubahan jaringan testikular mencit akibat pemberian ekstrak etanol biji pinang muda. Ekstrak etanol biji pinang muda disiapkan dengan maserasi, penjemuran, perendaman dan evaporasi hingga diperoleh serbuk ekstrak etanol yang kemudian dilarutkan dalam larutan Gum Arab 1% sesuai dosis. Mencit jantan galur Swiss Webster (umur8 minggu, berat 30-35 gram) dibagi dalam 5 kelompok perlakuan: (1) K = Kontrol, (2) KP = Kontrol pelarut (Gum Arab 1%), (3) P1 = Perlakukan 1 (dosis 300 mg/kg berat badan (bb)), (4) P2= Perlakuan 2 (dosis 500 mg/kg bb), dan (5) P3=Perlakuan 3 (dosis 700 mg/kg bb), diberikan secara oral (gavage) selama 17 hari. Mencit ditimbang sebelum perlakuan dan setelah perlakuan. Mencit dibunuh pada hari ke 18 dengan dislokasi leher, testis diisolasi, ditimbang dan difoto untuk mengetahui penambahan berat dan ukuran. Preparat histologi sayatan testis disiapkan dengan pewarnaan HE dan TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay untuk mengamati apoptosis. Hasil pengamatan menunjukkan terjadi (1) penurunan berat relatif testis kelompok P3, (2) ukuran testis relatif lebih kecil pada kelompok perlakuan, (3) perbedaan struktur jaringan testis pada kelompok P2 dan P3, al: (a) jumlah tubulus seminiferus lebih sedikit, (b) diameter tubulus seminiferus lebih kecil, (c) jarak antar tubulus seminiferus lebih renggang, (d) penurunan jumlah sel testikular, dan (e) peningkatan jumlah sel yang mengalami apoptosis. Ekstrak etanol biji pinang menyebabkan peningkatan apoptosis dan memiliki potensi sebagai agen alami antifertilitas pria. 

Oral Administration of Cogongrass (Imperata cylindrica L) Root Ethanol- Extract causes Mouse Epididymal Sperm Abnormality (PEMBERIAN EKSTRAK ETANOL AKAR ALANG-ALANG (IMPERATA CYLINDRICA L) SECARA ORAL MENYEBABKAN ABNORMALITAS SPERMA EPIDIDYMIS MENCIT)

Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Sperm morphology is an important parameter to be observed in the male fertility. Some of the bioactive compounds of cogongrass root such as alkaloid and terpenoid, affect male fertility by interference the spermatogenesis. The objective of the study was to observe the effect of cogongrass root ethanol extract on mouse sperm morphology. This study was carried out by oral administration of two different doses i.e 90 and 115 mg/kg body weight of cogongrass root ethanol extract into 8-10 weeks old DDY strain mice for 14 days to evaluated the acute effect due to the administration of cogongrass root ethanol extract on mouse sperm morphology. The results showed that treatment with cogongrass root ethanol extract significantly increased sperm abnormalities followed a dose depending pattern (p<0.05). Interestingly, the administration of cogongrass root extract did not affect sperm head morphology but tailless, folded and bent sperm increased linearly with the administration dose of cogongrass root ethanol extract. In conclusion, cogongrass root ethanol extract causes  secondary sperm abnormalitties on mouse sperm. 

Hubungan Reactive Oxygen Species (ROS), Superoxide Dismutase (SOD) dengan Protein α-Sinuklein-Larut Air pada Batang Otak Tikus yang Diinduksi Rotenon

Global Medical & Health Communication (GMHC) Vol 3, No 2 (2015)
Publisher : Faculty of Medicine Universitas Islam Bandung

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Abstract

Parkinson adalah salah satu penyakit neurodegeneratif dengan ganggunan gerak bila kematian neuron dopaminergik lebih dari 70%. Paparan neurotoksin diduga menjadi penyebab terjadinya Parkinson sporadik. Tujuan penelitian ini adalah menentukan kondisi stres oksidatif pada batang otak tikus Wistar yang diinduksi rotenon. Metode penelitian eksperimental dengan menggunakan tikus Wistar jantan. Penelitian dilakukan di Sekolah Ilmu dan Teknologi Hayati (SITH) periode Januari 2011–November 2013. Variabel bebas yaitu kelompok tikus, lama perlakuan, waktu pengamatan, dan lokasi di batang otak. Variabel terikat yaitu konsentrasi superoxide dismutase (SOD), konsentrasi protein α-sinuklein-larut air, dan densitas reactive oxygen species (ROS). Densitas ROS berbeda secara signifikan antara kelompok perlakuan (p=0,029), waktu pengamatan (p=0,0001), dan lokasi di batang otak (p=0,001). Konsentrasi SOD tidak berbeda secara signifikan antarkelompok perlakuan (p=0,566), waktu pengamatan (p=0,441), dan lokasi di batang otak (p=0,091). Konsentrasi protein α-sinuklein-larut air berbeda secara signifikan antarkelompok perlakuan (p=0,001) dan waktu pengamatan (p=0,001), tetapi tidak berbeda secara signifikan pada lokasi di batang otak (p=0,625). Densitas ROS relatif tertinggi pada hari ke-10 dan ke-40. Sementara itu, konsentrasi SOD pada hari ke-10 dan ke-40 relatif rendah, sedangkan konsentrasi protein α-sinuklein-larut air pada hari ke-10 relatif tinggi dibanding dengan hari ke-40. Dengan demikian, dapat disimpulkan bahwa stres oksidatif pada batang otak tikus Wistar yang diinduksi rotenon berpengaruh pada struktur protein α-sinuklein. THE RELATIONSHIP AMONG REACTIVE OXYGEN SPECIES (ROS), SUPEROXIDE DISMUTASE (SOD), AND α-SYNUCLEIN PROTEIN-WATER SOLUBLE IN WISTAR RAT'S BRAINSTEM TREATED WITH ROTENONEParkinson is the neurodegenerative disease with movement disordered, if the dopaminergic neurons dead more than 70%. Neurotoxins exposure is predicted cause sporadic Parkinson. The research aim was to determine oxidative stress stage in the brainstems Wistar rat’s treated-rotenone. An experimental study using male Wistar rats. The study was held in School of Life Sciences and Technology during January 2011–November 2013. The independent variables: groups of rats, long treatment, observation time, and location in the brainstem. The dependent variables: superoxide dismutase (SOD) concentration, concentration of protein α-synuclein-water soluble, and reactive oxygen species (ROS) density. Reactive oxygen species (ROS) density significantly different among treatment groups (p=0.029), observation time (p=0.0001), and the location in the brainstem (p=0.001). Superoxide dismutase (SOD) concentrations were not significantly different among treatment groups (p=0.566), observation time (p=0.441), and the location in the brainstem (p=0.091). The concentration of protein α-synuclein-water soluble was significantly different among treatment groups (p=0,001) and observation time (p=0.001), but not significantly different at the location in the brainstem (p=0.625). Reactive oxygen species (ROS) density were relatively high at day 10 and 40. Meanwhile SOD concentration on day 10 and 40 are relatively low. The concentration of α-synuclein protein-water soluble on day 10 was relatively higher than on day 40. The conclution is oxidative stress in the brainstem Wistar rat’s treated-rotenone effected on the protein α-synuclein structure changes.