Titiek Sumarawati
Department of Biomedical Sciences, Medical Faculty, Sultan Agung Islamic University Raya Kaligawe Street KM. 4. Semarang, Central Java Indonesia

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Pengaruh Tempe terhadap Kemampuan Fagositosis Makrofag Studi Eksperimental pada Mencit Jantan Strain Balb/c afiyata, noven; Sarosa, Hadi; Sumarawati, Titiek
Jurnal Sains Medika Vol 3, No 1 (2011)
Publisher : Fakultas Kedokteran UNISSULA

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Abstract

Background: Consuming tempeh regularly increases the levels of isoflavones in the our body influencingthe IFNγ and macrophage activating factor (MAF) that lead to the in increase in the ability of macrophagephagocytosis. Macrophage phagocytosis gives an illustration of the macrophages ability as the first lineof phagocytic cells to digest foreign particles. This study aims at finding out the influence of tempeh onthe ability of macrophage phagocytosis.Design and Methods: In an experimental study using post test only control group design, 15 mice Balb/c were divided into 3 groups of 5 mice each. Gp1: 0.5 gr of tempeh juice, Gp2: 1 mg tablet imboost in 1 ccof water (positive control), Gp3: 1 cc of 1 times (negative control). Treatment was given daily for 12 days.On day 13, Phagocytic ability of macrophages were examined using latex beads method. Data wereanalyzed using one Kruskal Wallis test followed by Independent T-Test.Results: The phagocytic ability for the three groups were 760,6 -----± 109,898 ; 244.2 ± 70.159; 9.6 ± 2.839respectively. Independent t- test resulted in the significance difference (p = 0.008) between treated groupsand control groups, both positive and negative ones (p<0,05).Conclusion: There is a difference in phagocytic ability between treatment and control groups (Sains Medika,3(1):54-62).ABSTRAKLatar belakang: Konsumsi tempe secara teratur dapat meningkatan kadar isoflavon dalam tubuh yangmampu mempengaruhi IFNγ dan Macrophage Activating Factor (MAF) sehingga menyebabkanpeningkatan kemampuan fagositosis makrofag. Fagositosis makrofag memberi gambaran mengenaikemampuan makrofag sebagai sel fagosit lini pertama untuk mencerna partikel asing. Tujuan penelitainini untuk mengetahui pengaruh tempe terhadap kemampuan fagositosis makrofag.Metode: Jenis penelitian eksperimental dengan rancangan post test only control group designmenggunakan15 ekor mencit strain Balb/c yang dilakukan random sampling dan dibagi menjadi 3kelompok, terdiri dari 5 ekor mencit. Kelompok perlakuan: 0,5 gram jus tempe/ hari, kelompok kontrolpositif : 1 mg imboost tablet dalam 1 cc air/ hari, kelompok kontrol negatif : pemberian 1 cc/ hari.Perlakuan dilakukan selama 12 hari. Pemeriksaan kemampuan fagosit makrofag dilakukan pada harike-13 dengan latex beads method dan data yang diperoleh di uji dengan Uji Kruskal Wallis dilanjutkandengan T-Test Independent.Hasil: Kemampuan fagosit mnakrofag pada kelompok perlakuan sebesar 760,6 -----± 109,898; kelompokkontrol positif 244,2 ± 70,159 kelompok kontrol negatif 9,6 + 2,839. Hasil uji T-Test Independentmenunjukkan terdapat perbedaan yang signifikan antara kelompok perlakuan dengan kelompokkontrol, baik positif maupun negative (p<0,05).Kesimpulan: Terdapat perbedaan rerata kemampuan fagosit antara kelompok perlakuan dan kelompokkontrol (Sains Medika, 3(1):54-62).
Efek Ekstrak Tanin Buah Pisang Kluthuk (Musa balbisiana Colla) sebagai Antiplasmodia Sumarawati, Titiek; Hussaana, Atina
Jurnal Sains Medika Vol 2, No 1 (2010)
Publisher : Fakultas Kedokteran UNISSULA

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ABSTRACTBackground: Malaria, the best-known tropical disease is said to be the most important parasitic diseasethat afflicts humans today. Recently, studies show the Increasing antimalarial drug resistance. Thus,alternative antimalarial compound need to be examined to find a new antiplasmodial compound includingtannin. This study aimed at finding out the effect of tannin extract isolated from pisang kluthuk or Musabalbisiana on the parasitemia in Plasmodium berghei infected Balb/c mice.Design and Method: In this post test only control group design study, 24 male Balb/c mice were randomlyassigned to receive orally administered aquadest, extract of pisang klutuk of 50% or 75% or 100% for 10days once daily. Parasitemia blood level in mice on day 5 for the four groups were 32.07%, 2.43%, 1.35%,and 0.32% respectively; whereas parasitemia blood level in mice on day 10 were 39.45%, 1.13%, 0.47%, and0.20% respectively.Result: One-Way Anova shows difference in parasitemia level among the treated groups (p< 0.05). Extractof Musa balbisiana colla lowers the level of parasitemia in Balb/C mice infected by Plasmodium berghei.Conclusion: The 100% consentration was shown to have more effect compared to 75% and 50% (SainsMedika, 2(1): 8-14).ABSTRAKPendahuluan: Malaria merupakan suatu penyakit infeksi parasitik yang paling penting dan masih menjadimasalah penyakit tropis di dunia. Akhir-akhir ini mulai banyak diketahui bahwa telah terjadi peningkatanresistensi terhadap obat antimalaria, sehingga perlu dilakukan eksplorasi senyawa antiplasmodia baru,salah satunya dari tanin. Penelitian ini bertujuan untuk mengetahui efek ekstrak tanin buah pisangkluthuk (Musa balbisiana colla) terhadap penurunan parasitemia mencit balb/c yang diinfeksi Plasmodiumberghei.Metode Penelitian: Rancangan penelitian yang digunakan adalah post test only control group design.Sebanyak 24 ekor mencit Balb/c jantan terbagi dalam 4 kelompok perlakuan, yaitu: K1 sebagai kontrolhanya diberi aquades, P1 diberi ekstrak pisang kluthuk konsentrasi 50% (EPK 50%), P2 diberi EPK 75%,dan P3 diberi EPK 100%. Pemberian perlakuan dilakukan secara oral selama 10 hari, dengan frekuensipemberian 1 kali sehari. Tingkat parasitemia darah mencit pada hari ke-5 dan ke-10 pada K1, P1, P2, P3pada hari ke-5 masing-masing sebesar 32,07%, 2.43%, 1.35%. dan 0.32%; sedangkan pada hari ke-10masing-masing sebesar 39,45%, 1.13%, 0.47%, dan 0.20%.Hasil Penelitian: Hasil analisis One-Way Anova menunjukkan terdapat perbedaan tingkat parasitemiadari keempat kelompok perlakuan (p< 0,05). Ekstrak pisang kluthuk (Musa balbisiana colla) dapatmenurunkan parasitemia mencit Balb/C yang diinfeksi Plasmodium berghei.Kesimpulan: Efek penurunan parasitemia setelah pemberian ekstrak pisang kluthuk konsentrasi 100%lebih besar daripada ekstrak pisang klutuk konsentrasi 75 % maupun 50 % (Sains Medika, 2(1): 8-14).
The Effect of Fasting on the Concentration of Enzimatic Antioxidants (Superoxide Dismutase and Glutathione Peroxidase) in Rats Jannah, Muliatul; Nasihun, Taufiqurrahman; Sumarawati, Titiek
Sains Medika Vol 7, No 1 (2016): January - June 2016
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26532/sainsmed.v7i1.1166

Abstract

 Introduction: Consumption of excessive calories can increase the incidence of degenerative diseases mediated by ROS. Caloric restriction, have been shown to increase levels of antioxidants superoxide dismutase (SOD) and Gluthatione Peroxidase (GPx). Fasting like Ramadan fasting (FLRF) is a form of calorie restriction, but its effect on levels of SOD and GPx remains unclear. Objectives: to investigate the effect of fasting on levels of SOD and GPx. Methods: in a post-test only control group design, sample of 24 rats Sprague Dawley Rats aged 3-month-old, weighing 250-300 grams, were randomly divided into 4 groups. Group 1 (P-70), 2 (P-100), and 3 (P-140) were fasted for 6 hours/day, each group received of 70%, 100% and 140% calories respectively. Group 4 (C-AL) received 100% calories, ad libitum. Day 16 blood was taken and levels of SOD and GPx were determined by ELISA. Data were analyzed using one-way ANOVAs, followed by post hoc LSD tests, p<0.05 was considered statistically significant. Results: the results showed that the levels of SOD and GPx occur significant differences between the groups, p = 0.000. The test results post hoc SOD (318.64) and GPx (89.16) group P-70, compared with group C-AL (278.60 and 57.20) was significantly higher (p = 0.00). SOD and GPx P-70 group compared with the group P-140 (92.03 and 48.79), significantly higher (p = 0.00). Compared with group P-100 (296.70 and 75.71) SOD and GPx in group P-70 was significantly higher, p = 0.000. Conclusion: Fasting with calorie intake of 70% and 100% for 15 days can increases levels of SOD and GPx in male rats.
Risk Factors of Acute Respiratory Infections in Practice Area for Community of Medical Students in Semarang Zulaikhah, Siti Thomas; Soegeng, Purwito; Sumarawati, Titiek
Jurnal Kesehatan Masyarakat Nasional Volume 11, Issue 4, May 2017
Publisher : Faculty of Public Health Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21109/kesmas.v11i4.1281

Abstract

Acute respiratory infection (ARI) ranks first of 10 major diseases in Primary Health Care Bangetayu with the highest percentage in Penggaron Lor Subdistrict. Skill to learn distribution and frequency of diseases as well as determinant factors that affect human health is needed in determine the most effective intervention to increase public health level. This study aimed to determine dominant factors related to ARI incidence in location of practice for community of medical students of Islam Sultan Agung University, Semarang. This study used cross-sectional design with 100 respondents and the samples were collected by stratified random sampling. Ten variables examined were environmental sanitation risk factors, while six related to behavior and health care. Data analysisused a chi-square test (bivariate) and multiple regression logistic (multivariate). Environmental sanitation factors were significantly related to ARI including the presence of ventilation, smoke hole kitchen, bedroom, residential density and the most dominant factor was the habit of smoker family members in Penggaron Lor Subdistrict. This location can be used as a practice area for the community of medical students who take Public Health Studies due to complex health problems.AbstrakInfeki saluran pernapasan akut (ISPA) menempati urutan pertama dari 10 besar penyakit di Puskesmas Bangetayu dengan persentase terbanyak di Kelurahan Penggaron Lor. Keterampilan untuk mempelajari distribusi dan frekuensi penyakit serta faktor determinan yang memengaruhi manusia sangat diperlukan untuk menetapkan intervensi yang paling efektif untuk meningkatkan derajat kesehatan masyarakat. Perlu dilakukan penelitian untuk mengetahui faktor risiko yang paling dominan berhubungan dengan kejadian ISPA di lokasi praktik komunitas mahasiswa Fakultas Kedokteran Universitas Islam Sultan Agung Semarang. Penelitian ini menggunakan desain potong lintang dengan jumlah responden 100 orang dan sampel dikumpulkan dengan menggunakan stratified random sampling. Sepuluh variabel yang diteliti adalah faktor risiko sanitasi lingkungan, sedangkan enam faktor risiko terkait dengan perilaku dan pelayanan kesehatan. Data dianalisis secara bivariat dengan uji kai kuadrat dan multivariat dengan regresi logistik ganda. Faktor yang berhubungan dengan kejadian ISPA adalah ventilasi, lubang asap dapur, ruang tidur, dan kepadatan hunian. Faktor yang paling dominan adalah kebiasaan anggota keluarga yang merokok di Kelurahan Penggaron Lor. Lokasi ini dapat digunakan sebagai lahan praktik komunitas bagi mahasiswa kedokteran yang sedang kepaniteraan di program studi Ilmu Kesehatan Masyarakat karena memiliki permasalahan kesehatan yang kompleks.
Effect of White Turmeric Extract (Curcuma zedoaria) Using Zam-zam Solvent Compare with Ethanol Solvent Against Breast Cancer Cell T47D Hudaya, Isna; Nasihun, Taufiqurrahman; Sumarawati, Titiek
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 6, No 2 (2015): July-December 2015
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26532/sainsmed.v6i2.601

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Introduction: Curcuma zedoaria is one of the herbal plants potentially protects and cures many diseases, particularly as anti-cancer and anti-tumor. Anti-cancer active compounds of it are flavonoid, phenolic, and saponin. Objectives: This study aimed to explore the cytotoxicity of Curcuma zedoria extract (CZE). Methods: Experimental Quasi with post test non equivalent control group design on T47D cell line at Biology laboratory, Faculty of Medicine, Sultan Agung Islamic University, Semarang. The research was consisted of 2 groups, namely: intervention group with CZE zam-zam solvent and intervention group with CZE ethanol solvent given 10 different dosages each for 1.000 μg/mL; 500 μg/mL; 250 μg/mL; 125 μg/mL; 62.5 μg/mL; 31.25 μg/mL; 15.62 μg/mL; 7.81 μg/mL; 3.90 μg/mL; 1.95 μg/mL. Cytotoxicity test of IC50 using direct counting method and analyzed by probit analysis. Results: IC50 value of CZE in zam-zam and ethanol solvent were 28.24 μg/ml and 13.71 µg/ml respectively at the same 24 hours incubation period. Chi-square test revealed p value was 0.000 (α = 0.05), meaning that there was significant relationship. Conclusion: CZE activity using ethanol is highly active while CZE using zam-zam water is moderate and both of them have the toxicity on breast cancer cell. As the result, apoptosis process may occur.
Studi Salinitas Air terhadap Penyerapan Logam Berat Merkuri oleh Ikan Mujair (Tilapia mosambicca) Sumarawati, Titiek
Jurnal Kimia Sains dan Aplikasi Vol 2, No 3 (1999): Volume 2 Issue 3 Year 1999
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.2.3.90-98

Abstract

Merkuri adalah logam berat yang toksik didam jumlah kecil, akan tetapi toksisitasnya bervariasi tergantung dari bentuk senyawa kimianya dan tempat pemaparannya. Dalam perairan, kandungan logam merkuri sekalipun kadarnya relatif rendah, dapat diabsorbsi dan terakumulasi secara biologis oleh ikan dan akan terlibat dalam sistim jaringan makanan. Pengamatan penelitian ini melihat kondisi air tambak oso yang meliputi suhu, pH, salinitas, kandungan merkuri dalam air dan kandungan merkuri dalam ikan mujair. Jenis penelitian ini adalah penelitian analitik yang bersifat observasional, yang dilakukan secara cross-sectional. Hasil dari penelitian ini menunjukkan bahwa kandungan logam merkuri dalam air rata rata sebesar 0,0024 ppm, di mana suhu berfluktasi, pH dan salinitas adalah relatif rendah. Rata rata suhu dari air 28,23°C, pH 7,28 dan salinitas 0,3084 o/ooo. Merkuri dalam sampel ikan mujair rata-rata sebesar 0,00084 ppm. Sampel ikan mujair yang berumur 3 bulan. 4 bulan dan 5 bulan mempunyai kandungan merkuri yang berbeda
The Effect of Tempeh on the Ability of Macrophage Phagocytosis An experimental study in male Balb/c mice Afiyata, Noven; Sarosa, Hadi; Sumarawati, Titiek
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 3, No 1 (2011): Januari-Juni 2011
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26532/sainsmed.v3i1.409

Abstract

Background: Consuming tempeh regularly increases the levels of isoflavones in the our body influencing the IFNã and macrophage activating factor (MAF) that lead to the in increase in the ability of macrophage phagocytosis. Macrophage phagocytosis gives an illustration of the macrophages ability as the first line of phagocytic cells to digest foreign particles. This study aims at finding out the influence of tempeh on the ability of macrophage phagocytosis.Design and Methods: In an experimental study using post test only control group design, 15 mice Balb/c were divided into 3 groups of 5 mice each. Gp1: 0.5 gr of tempeh juice, Gp2: 1 mg tablet imboost in 1 cc of water (positive control), Gp3: 1 cc of 1 times (negative control). Treatment was given daily for 12 days. On day 13, Phagocytic ability of macrophages were examined using latex beads method. Data were analyzed using one Kruskal Wallis test followed by Independent T-Test.Results: The phagocytic ability for the three groups were 760,6 -----± 109,898 ; 244.2 ± 70.159; 9.6 ± 2.839 respectively. Independent t- test resulted in the significance difference (p = 0.008) between treated groups and control groups, both positive and negative ones (p<0,05).Conclusion: There is a difference in phagocytic ability between treatment and control groups (Sains Medika, 3(1):54-62).
The Study of Mouth Washing Duration with Boiled Uncaria gambir on the Dental Plague Formation Susilowati, Aning; Sumarawati, Titiek
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 4, No 1 (2012): Januari-Juni 2012
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26532/sainsmed.v4i1.385

Abstract

Background: Dental plague has been known to be the major agent causing dental carries and periodontal disease which are the most common dental and soft tissue diseases with the prevalence of 70-80%. The preventive measure including the use of chemical substance of catechin having anti-plague activity in the form of mouth wash has been shown to have effects on the dental plague formation. The objective of this study was to find out the duration of mouth washing with boiled gambir (Uncaria ambir) on the plague formation in Pondok Pesantren Al-Badriyah Mranggen, Demak.Design and Method: In this study using Pretest-Posttest Control Group Design, 44 samples of subjects was divided into 4 groups of 11 each. Group I, II, II were treated with the mouth wash of Uncaria gambir boiled 2, 3, 4 minutes respectively. Group IV serving as control was treated with mouth wash of water for 3 minutes. The data was analyzed with non parametric test using Kruskal-Wallis followed by Mann-Whitney test with p < 0.05.Result: Kruskal Wallis analysis showed a significant difference in mean of plague before and after the treatment among the four groups (p < 0.05). Mann-Whitney test showed no significant difference between group I and III ( p> 0.05) and significant difference for the rest of the groups (p < 0.05). Conclusion: The mouth rinse duration using boiled Gambir has been shown to have an effect on the dental plague formation. 3 minute mouth washing shows the optimum effect (Sains Medika, 4(1):57-62).
The effect of Gandarusa Leaf extract (Justicia gendarussa Burm F.) Administration on Estradiol Hormone Level and the Amount of Antral Ovarium Follicle on Female Mice Laili, Ni'mah Hidayatul; Nasihun, Taufiqurrachman; Sumarawati, Titiek
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 9, No 2 (2018): July-Desember 2018
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/sainsmed.v9i2.2099

Abstract

 Background: Physiologically, woman experiencing aging characterized by menopause. Gandarusa leaf contains isoflavon, a phytoesterogen expected to be used as esterogen replacement therapy. Objective: the aim of this research is to investigate the administrative effect of gandarusa leaf extract on the increase of estradiol hormone level and the number of antral ovarium follicle of female mice.Methods: This is experimental study with posttest only control group research design. Samples are 24 balb-c female mice, aged 16-17 months, weighed 19-35 grams, divided into 4 groups. Control group (Ctrl-G) were given 0.48 ml aquadest; group JB10-G, JB20-G, and JB30-G, each were given gandarusa leaf extract with concentration 10%, 20%, dan 30% in volume 0.48 ml orally twice a day. After treatment, dissection were conducted to make ovarium histology preparation with Hematoxylin Ehrlich-Eosin staining, observation were conducted using microscope. Results: Post hoc analysis indicated that the estradiol level and the number of antral ovarium follicle on administration of gandarusa leaf extract with concentration 10%, 20%, dan 30% are significantly higher, p < 0.05. The increase of estradiol level with extract concentration of 10% are significantly negatively correlated with the number of antral ovarium follicles p<0.05. Conclusion: The administration of gandarusa leaf extract increase the estradiol hormone level and the number of antral ovarium follicle in female mice. Keywords : gandarusa leaves extract, estradiol endogenous, ovary histology
Hypoxia enhances self-renewal properties and markers of mesenchymal stem cells Yustianingsih, Vivi; Sumarawati, Titiek; Putra, Agung
Universa Medicina Vol 38, No 3 (2019)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2019.v38.164-171

Abstract

BackgroundMesenchymal stem cells (MSCs) are multipotent stromal cells that express CD73, CD90, and CD105 surface markers, but not CD14, CD45, CD34, CD11b, and HLA-DR. MSCs under hypoxic conditions have the essential role of maintaining the stemness capacity by releasing several growth factors into their medium, known as hypoxia conditioned medium (HCM). This study was performed to compare the effect of percentage of HCM to normoxic medium (NM) in increasing MSC proliferation marked by proliferation rate and surface marker expression.MethodsThis study was of post-test only control group design using human umbilical cord-MSCs (hUC-MSCs) as subjects. The HCM treatment group was obtained by culturing MSCs under 5% O2, whereas the NM control group was grown under 20% O2. The hUC-MSCs were divided into 4 groups with different dose ratios of HCM to NM (25%:75%; 50%:50%; 75%:25% for P1, P2 and P3, respectively and 100% of NM for the controls). All of these groups were maintained at 37oC and the data was collected after 72 hours incubation. MSC marker expression of CD73, CD90 and CD105 was analyzed using flow cytometry and MSC proliferation by trypan blue assay. ResultThere were significant differences in MSC marker expression of CD73, CD90 and CD105 and proliferation at all dose ratios of HCM to NM (p&lt;0.05).ConclusionLow oxygen concentration promotes MSC proliferation and stemness thus it might be beneficial for maintaining the MSC physiologic niche in-vitro.