C. SUMANTRI
Departemen Ilmu Produksi dan Teknologi Peternakan, Institut Pertanian Bogor

Published : 52 Documents
Articles

PENDUGAAN BOBOT BADAN MELALUI ANALISIS MORFOMETRIK DENGAN PENDEKATAN REGRESI TERBAIK BEST - SUBSET PADA DOMBA GARUT TIPE PEDAGING, TANGKAS DAN PERSILANGANNYA GUNAWAN, A.; JAMAL, K.; SUMANTRI, C.
Majalah Ilmiah Peternakan Vol 11, No 1 (2008)
Publisher : Majalah Ilmiah Peternakan

Show Abstract | Original Source | Check in Google Scholar

Abstract

ABSTRACT ESTIMATE OF BODY WEIGHT FIGHTING AND MEAT GARUT SHEEP AND CROSSBREED WITH MERFOMETRIC ANALYSIS APPROACH The aims of this study were to estimate body weight and to determine the best equation which can be used to estimate body weight of fighting and meat Garut sheep and its crossbreed type at district and outdistrict Garut. This study used primary data of Garut sheep from Garut district consisting of Margawati, Wanaraja and Sukawening. For outdistrict Garut, it was used Garut sheep from Bogor consisting of Ciomas and Cinagara. The total number of sheep collected were 531 heads. The differences of body measurements locations were analyzed by t-test, Correlation, and Regression Multiple Linear Regression and Polynomial Regression (Linear, Quadratic, and Cubic) Analyses. The results showed that body weight of fighting and meat rams and ewes from Wanaraja were the highset than another Garut sheep and crossbreed. The highest body measurements were found from Wanaraja (fighting and meat type) and Sukawening (fighting type) sheep and the lowest body measurements were found from Margawati and Cinagara. Whilst that some body measurements of rams were not different (P>0.05). Correlation coefficient between body weight and various body measurements body lenght, weither height, chest deep and chest circumference of Garut Sheep were highest at district and outdistrict of Garut. The best regression equations to describe the relation ship between body weight and body measurements for rams and ewes Garut sheep at district and outdistrict Garut were multiple regression equations. The highest determination coefficient (100%) was shown for meat type from Wanaraja and the lowest one (93.93%) was shown of fighting type from Sukawening. ABSTRAK Penelitian ini bertujuan untuk menduga bobot badan dan mencari model terbaik dalam menduga bobot badan domba Garut tangkas, pedaging dan persilangannya di daerah Garut dan luar Garut. Total domba yang digunakan pada penelitian ini sebanyak 531 ekor domba Garut dari beberapa lokasi di kabupaten Garut (Margawati, Wanaraja, Sukawening) dan di Luar Garut (Ciomas dan Cinagara). Data dari hasil penelitian dianalisis dengan menggunakan Uji-t, Korelasi, dan Regresi meliputi Analisis Regresi Linier Ganda dan Analisis Regresi Polinomial (Linier, Kuadratik, dan Kubik). Hasil Uji-t menunjukkan, bahwa rataan bobot badan domba tangkas Garut baik jantan maupun betina dari Wanaraja memiliki bobot badan paling besar dibandingkan dengan domba Garut lain dan persilangannya. Ukuran tubuh tertinggi pada domba Garut ditunjukkan pada domba tangkas dan pedaging Wanaraja serta tangkas Sukawening dan ukuran tubuh terkecil ditunjukkan pada domba Garut di Margawati dan Cinagara. Koefisen Korelasi tertinggi antara bobot badan dan ukuran-ukuran tubuh pada domba Garut tangkas, pedaging da persilanganya ditunjukkan antara bobot badan dengan panjang badan, tinggi pundak, dalam dada dan lingkar dada. Persamaan regresi yang paling baik digunakan menduga bobot badan berdasarkan ukuran-ukuran tubuh pada domba Garut tangkas, pedaging dan persilangannya adalah Persamaan Regresi Linier Ganda dengan tingkat akurasi berkisar antara 93,3%-100%. Tingkat akuras tertinggi ditunjukkan pada domba tangkas Wanaraja dengan tingkat akurasi 100% dan terendah ditunjukkan pada domba tangkas di Sukawening dengan tingkat akurasi 93,93%.
Hubungan Keragaman Gen Leptin dengan Kualitas Fisik Daging Sapi Lokal Di Ciamis (Relationship between Leptin Gene Diversity with Physical Quality of Local Beef In Ciamis) Hilmia, Nena; Noor, R.R; Sumantri, C.; Priyanto, R.; E, Gurnadi
Jurnal Ilmu Ternak Universitas Padjadjaran Vol 15, No 2 (2015)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Original Source | Check in Google Scholar | Full PDF (483.972 KB)

Abstract

Leptin  terlibat  dalam  berbagai  proses  fisiologispembentukan  lemak.  Keragaman  gen Leptin  karena  adanya  SNP  Arg25Cys  pada  exon  2,  mempunyai  hubungan  dengan  deposisi lemak, yang dapat mempengaruhi  kualitas daging. Penelitian  ini bertujuan  untuk mengetahui keragaman gen leptin  dan hubungannya dengan kualitas fisik daging sapi lokal di Ciamis Jawa Barat.  Penelitian  ini  menggunakan  14  sampel  daging  bagian  udamaru  yang  diperoleh  dari peternakan  rakyat.Sampel  DNA diisolasi  dari daging.  Amplifikasi  DNA menggunakan  PCR, dan   penentuan   genotipe   dianalisis   dari   hasil   sequencing   produk   PCR.   Hasil   penelitian menunjukkan Gen Leptin pada sapi lokal Ciamis bersifat polimorfik, terdapat tiga alel, yaitu C, T (memiliki mutasi Arg25Cys) dan H (memiliki mutasi Arg25His) serta terdapat tiga genotipe CC,  CT  dan  CH.  Perbedaan  genotipe  gen  Leptin  tidak  berpengaruh  terhadap  kualitas  fisik daging sapi lokal di Ciamis.Kata kunci: Kualitas daging, Leptin
Karakteristik Morfometrik Ukuran Tubuh dan Bentuk Domba Ekor Gemuk Pulau Madura dan Rote dengan Menggunakan Analisis Komponen Utama Gunawan, A.; Sumantri, C.
Buletin Peternakan Vol 31, No 4 (2007): Buletin Peternakan Vol. 31 (4) November 2007
Publisher : Fakultas Peternakan UGM

Show Abstract | Original Source | Check in Google Scholar | Full PDF (823.855 KB)

Abstract

File lengkap ada dalam bentuk PDF dibawah ini
Polymorphism of growth hormone receptor (GHR) gene in Holstein Friesian dairy cattle Misrianti, Restu; Sumantri, C.; Anggraeni, A.
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 4 (2011): DECEMBER 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Original Source | Check in Google Scholar | Full PDF (297.675 KB)

Abstract

Growth hormone gene have a critical role in the regulation of lactation, mammary gland development and growth process through its interaction with a specific receptor. Growth hormone (GH) is an anabolic hormone which is synthesized and secreted by somatotrop cell in pituitary anterior lobe, and interacts with a specific receptor on the surface of the target cells. Growth hormone receptor (GHR) has been suggested as candidate gene for traits related to milk production in Bovidae. The purpose of this study was to identify genetic polymorphism of the Growth Hormone Receptor (GHR) genes in Holstein Friesian (HF) cattle. Total of 353 blood samples were collected from five populations belonging to Cikole Dairy Cattle Breeding Station (BPPT-SP Cikole) (88 samples), Pasir Kemis (95 samples), Cilumber (98 samples), Cipelang Livestock Embryo Center (BET Cipelang) (40 samples), Singosari National Artificial Insemination Centre (BBIB Singosari) (32 samples) and 17 frozen semen samples from Lembang Artificial Insemination Center (BIB Lembang). Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques then PCR products were genotyped by the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) methods. There were two allele dan three genotypes were found namely: allele A and G, Genotype AA, AG and GG repectively. Allele A frequency (0.70-0.82) relatively higher than allele G frequency (0.18-0.30). Chi square test show that on group of BET Cipelang, BIB Lembang and BBIB Singosari population were not significantly different (0.00-0.93), while on group of BET Cipelang, BIB Lembang dan BBIB Singosari population were significantly different (6.02-11.13). Degree of observed heterozygosity (Ho) ranged from 0.13-0.42 and expected heterozygosity (He) ranged from 0.29-0.42. Key Words: Growth Hormone Receptor, Polymorphism, Holstein Friesian Cattle
Polymorphism of Pituitary-Specific Transcription Factor-1 (Pit-1) Gene at Locus (Pit-1-Hinf1) and its effects on dam body weight and milk production of local sheeps Sumantri, C.; Herdiana, D.; Farajallah, A.; Rahmat, D.
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 3 (2009): SEPTEMBER 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Original Source | Check in Google Scholar | Full PDF (302.36 KB)

Abstract

Pituitary-Specific Transcription Factor-1 (Pit-1) is a transcription factor with critical role in the transcriptional regulation of multiple genes in the pituitary. The objective of this research was to identify polymorphism of Pituitary-Specific Transcription Factor-1 (Pit-1) gene at Locus (Pit-1-Hinf1) and to investigate any possible associations of Pit-1 genotypes on dam body weight, milk production and milk quality in local sheep at the Jonggol Animal Science Teaching and Research Unit (JASTRU), Fact. Anim. Sci. Bogor. Agric. Univ. A total number of 161 blood samples were collected from 3 local sheep, namely Garut from Wanaraja (55 hd), Garut from Margawati (23 hd) and lactating ewes (83 hd) from JASTRU  farm in Bogor. Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques, then PCR products were digested with a Hinf1 enzyme restriction. Fragments of Pit-1gene at locus Pit-1-Hinf1 was detected by a silver-staining method. A length of 637 base pairs (bps) of the Pit-1 gene of local sheep was successfully amplified. The Hinf1 restriction enzyme cut the PCR product into three different length of fragments succesively at 345, 137, and 115   bps designated as A allele; whilst B allele had four fragments at 283, 137, 115, and 62 bps respectively. The locus of Pit-1-Hinf1 was polymorphic in local sheep from Jonggol, however it was monomorfic in Garut sheep. The frequencies of A and B alleles were 0,806 and 0,194 respectively. Pit-1 genotypes had no significant effect on dam body weight and milk production. This result is indicating that the use of single locus Pit-1-Hinf1 in Pit-1 gen is less effective to be used as a candidate in selecting dam body weight and milk production in these three local sheep. Key words: Local Sheep, Pit-1 Gene, Polymorphism, Dam, Milk
Verification for the control of kappa-casein gene on milk protein yield of friesian-holstein in dairy central region of West Java Anggraeni, A.; Sumantri, C.; Farajallah, A.; Andreas, E.
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 2 (2009): JUNE 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Original Source | Check in Google Scholar

Abstract

Genotipe of BB kappa kasein (ĸ-kasein) gene positively influences high milk protein yield. The aim of this research was to verify the use of the к-casein gene as marker of assisted selecion (MAS) of high protein yield in domestic Holstein-Friesian (HF) cattle. Genotyping blood samples was conducted for lactating cows from BPPT-SP Cikole (82 head) and a number of small holders in KPSBU Lembang (190 head). Genotyping the к-casein gene was also conducted for active and non active AI bulls (HF) from BIB Lembang (25 head) and BBIB Singosari (32 head). Varian genotipes were identified trough DNA migration, whereas frequencies of alleles were calculated by Nei Method (1987). The effects of variant genotypes on protein yield and other milk component yields were studied in lactating cows (56 head in BPPT-SP Cikole and 111 head in KPSBU Lembang) by applying Least Square Means (LSM) method of the General Linear Model (GLM). Genotyping the ĸ-kasein gene resulted in three genotypes, namely AA, AB and BB, with two alleles, namely A and B. It was identified that a very limited number of lactating cows with BB genotype (0-9%). These cows produced higher protein yield around 3.37-3.84% than that of AA cows, while AB cows produced protein yield in between, though these differences were not statistically significant (P>0,05). In contrast, AA cows tended to produced higher milk fat yield than BB cows. Cows BB and AB seemingly produced higher dry matter, while both specific density and pH were not affected by variant genotypes of the к-casein gene.  The results showed that BB genotype of the к-casein gene tended to be quitely consistent in controlling high protein yield, so it would be a good oppurtinity to be used as MAS of milk protein yield in HF domestic. Key words: Protein Yield, Dairy Cattle (HF), к-Casein Gene, Genotype BB
Polymorphism of stearoyl-CoA desaturase (SCD1) gene in Indonesian local cattle Hilmia, N.; Noor, R. R.; Sumantri, C.; Gurnadi, R. E.; Priyanto, R.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 1 (2017): March
Publisher : Diponegoro University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (107.456 KB)

Abstract

Stearoyl-Coa desaturase (SCD1) gene is one of genes that involves in converting saturated fatty acids to unsaturated fatty acids. SNP at exon 5 in SCD1 gene that changes amino acid valine to alanine (V293A) has an influence to meat fatty acid composition. The aim of this research was to analyze SCD1 gene polymorphisms based on SNP V293A at exon 5 of three Indonesian local cattle. The identification of SCD1 gene polymorphisms was done by using 98, 20 and 7 DNA sample from Ciamis, Bali/Banteng, and Ongole Grade (PO) cattle, respectively. PCR_RFLP method with AciI enzim was carried out to identify SNP Val293Ala. Allelic frequencies and heterozygosity value were analyzed by using POPGENE32. The result showed that SCD1 gene at Ciamis local cattle and PO cattle were polymorphic. Their frequencies were 74.5% and 71.4% for T and 25.5% and 28.6% for C, respectively. There were three genotypes on Ciamis local cattle i.e TT, CT and CC with their frequencies were 52%, 44.9% and 3.10%, respectively. There were two genotypes on PO cattle i.e TT and CT with their frequencies were 42.9% and 57.1%, respectively. Meanwhile, SCD1 gene in Bali cattle was monomorphic. Heterozygosity value of SCD1 gene in Ciamis and PO cattle were 0.38 and 0.44, respectively. Their heterozigosities were categorized as medium. 
Peningkatan produktifitas lebah madu melalui penerapan sistem integrasi dengan kebun kopi Saepudin, R.; Fuah, A. Fuah M.; Sumantri, C.; Abdullah, L.; Hadisoesilo, S.
Jurnal Ilmu-Ilmu Peternakan Vol 21, No 1 (2011)
Publisher : Fakultas Peternakan Universitas Brawijaya

Show Abstract | Original Source | Check in Google Scholar | Full PDF (405.962 KB)

Abstract

Abstract : The study of relationship between the honey productivity and honey bee-coffee plantation integration was conducted in Kepahiang, the Province of Bengkulu. The objective of this study was to evaluate the application of Apis cerana-coffee plant integration system on honey production and coffee bean as well.. The experiment was arranged in a completely randomized design with two treatments and ten replications. The result showed that honey production was higher by 114% than that outside the plantation. Similar to the honey productionn, coffee been production at honeybee-coffee plantation integration was significantly higher by 10,55 % than that was unpollinated by Apis cerana.Key words: cerana, coffee, integration. production
Verification for the control of kappa-casein gene on milk protein yield of friesian-holstein in dairy central region of West Java Anggraeni, A.; Sumantri, C.; Farajallah, A.; Andreas, E.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 2 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (202.262 KB)

Abstract

Genotipe of BB kappa kasein (ĸ-kasein) gene positively influences high milk protein yield. The aim of this research was to verify the use of the к-casein gene as marker of assisted selecion (MAS) of high protein yield in domestic Holstein-Friesian (HF) cattle. Genotyping blood samples was conducted for lactating cows from BPPT-SP Cikole (82 head) and a number of small holders in KPSBU Lembang (190 head). Genotyping the к-casein gene was also conducted for active and non active AI bulls (HF) from BIB Lembang (25 head) and BBIB Singosari (32 head). Varian genotipes were identified trough DNA migration, whereas frequencies of alleles were calculated by Nei Method (1987). The effects of variant genotypes on protein yield and other milk component yields were studied in lactating cows (56 head in BPPT-SP Cikole and 111 head in KPSBU Lembang) by applying Least Square Means (LSM) method of the General Linear Model (GLM). Genotyping the ĸ-kasein gene resulted in three genotypes, namely AA, AB and BB, with two alleles, namely A and B. It was identified that a very limited number of lactating cows with BB genotype (0-9%). These cows produced higher protein yield around 3.37-3.84% than that of AA cows, while AB cows produced protein yield in between, though these differences were not statistically significant (P>0,05). In contrast, AA cows tended to produced higher milk fat yield than BB cows. Cows BB and AB seemingly produced higher dry matter, while both specific density and pH were not affected by variant genotypes of the к-casein gene.  The results showed that BB genotype of the к-casein gene tended to be quitely consistent in controlling high protein yield, so it would be a good oppurtinity to be used as MAS of milk protein yield in HF domestic. Key words: Protein Yield, Dairy Cattle (HF), к-Casein Gene, Genotype BB
Polymorphism of Pituitary-Specific Transcription Factor-1 (Pit-1) Gene at Locus (Pit-1-Hinf1) and its effects on dam body weight and milk production of local sheeps Sumantri, C.; Herdiana, D.; Farajallah, A.; Rahmat, D.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 3 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (302.36 KB)

Abstract

Pituitary-Specific Transcription Factor-1 (Pit-1) is a transcription factor with critical role in the transcriptional regulation of multiple genes in the pituitary. The objective of this research was to identify polymorphism of Pituitary-Specific Transcription Factor-1 (Pit-1) gene at Locus (Pit-1-Hinf1) and to investigate any possible associations of Pit-1 genotypes on dam body weight, milk production and milk quality in local sheep at the Jonggol Animal Science Teaching and Research Unit (JASTRU), Fact. Anim. Sci. Bogor. Agric. Univ. A total number of 161 blood samples were collected from 3 local sheep, namely Garut from Wanaraja (55 hd), Garut from Margawati (23 hd) and lactating ewes (83 hd) from JASTRU  farm in Bogor. Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques, then PCR products were digested with a Hinf1 enzyme restriction. Fragments of Pit-1gene at locus Pit-1-Hinf1 was detected by a silver-staining method. A length of 637 base pairs (bps) of the Pit-1 gene of local sheep was successfully amplified. The Hinf1 restriction enzyme cut the PCR product into three different length of fragments succesively at 345, 137, and 115   bps designated as A allele; whilst B allele had four fragments at 283, 137, 115, and 62 bps respectively. The locus of Pit-1-Hinf1 was polymorphic in local sheep from Jonggol, however it was monomorfic in Garut sheep. The frequencies of A and B alleles were 0,806 and 0,194 respectively. Pit-1 genotypes had no significant effect on dam body weight and milk production. This result is indicating that the use of single locus Pit-1-Hinf1 in Pit-1 gen is less effective to be used as a candidate in selecting dam body weight and milk production in these three local sheep. Key words: Local Sheep, Pit-1 Gene, Polymorphism, Dam, Milk