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Infusa Daging Buah Mahkota Dewa Memperbaiki Kerusakan Testis dan Parameter Sperma Tikus Diabetik Mahkota Dewa Mesocarp Infusion Improved Testicular Damage and Sperm Count In Diabetic Rat Sulistyoningrum, Evy; -, Setiawati; Nindyastuti, Herlina; Putra, Adrian Nugraha
Jurnal Sains Medika Vol 4, No 2 (2012)
Publisher : Fakultas Kedokteran UNISSULA

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Abstract

Background: Global prevalence of Diabetes Mellitus (DM) is predicted to be increasing with worldwide populationincrease, longer life expectancy, urbanization, change in lifestyle and higher incidence of obesity. Diabetes Mellituswhich is characterized by increasing level of blood glucose can induce increasing activity of ROS (Reactive OxygenSpecies) which can further lead to abnormality in male reproductive system, especially testis. Mahkota dewa(Phaleria macrocarpa (Scheff.) Boerl.=PM) contain flavonoid, alkaloid and saponin which can prevent furthernegative effect on ROS increase in male reproductive system. The aim of this study was to know effect of PMmesocarp infusion on testicular damage and sperm count in alloxan induced diabetic rat.Design and Method: The design of this study was post test only with control group design. Rats in this study weredivided into 4 groups (6 rats each group): control group, diabetic group, diabetic group treated with PM mesocarpinfusion (9% and 13.5%). Diabetes was induced by alloxan 50 mg/BW single dose injection intramuscularly. PMmesocarp’s infusion was given in 4 weeks. Testicular damage was observed in hematoxylin-eosin stain usingJohnsen criteria for spermatogenesis level and tubulus seminiferous’ diameter. Sperm count was performed onthe semen taken from vas defferens using Giemsa stain.Result: PM mesocarp infusion group has higher spermatogenic level (p < 0,05), higher tubulus seminiferus diameterand sperm count (p < 0,05) than diabetic group.Conclusion: Mahkota dewa mesocarp infusion improved testicular damage and sperm count in alloxan-induceddiabetic rat (Sains Medika, 4(2):115-123).Pendahuluan: Prevalensi global Diabetes Mellitus diperkirakan akan semakin meningkat dengan peningkatanpopulasi global, angka harapan hidup yang semakin panjang, urbanisasi perubahan gaya hidup dan tingginyakejadian obesitas. Diabetes Mellitus yang ditandai dengan peningkatan kadar glukosa darah dapat menginduksipeningkatan aktivitas ROS (Reactive Oxygen Species) yang dapat menyebabkan abnormalitas pada saluranreproduksi maskulina, terutama testis. Daging buah Mahkota dewa (Phaleria macrocarpa (Scheff.) Boerl.) (PM)mengandung flavonoid, alkaloid dan saponin yang dapat mencegah dampak negatif lanjut akibat peningkatanROS pada sistem reproduksi maskulina. Tujuan penelitian ini adalah mengetahui efek infusa daging buah PMpada kerusakan testis dan jumlah sperma pada tikus diabetik yang diinduksi alloksanMetode Penelitian: Penelitian ini menggunakan pendekatan post test only with control group design. Tikus dibagisecara acak dalam 4 kelompok (masing-masing 6): kelompok kontrol, kelompok diabetik, kelompok diabetikmendapat infusa daging buah MD dosis 9% dan 13.5%. Diabetes diinduksi dengan injeksi alloxan 50 mg/kgBBdosis tunggal secara intramuskular. Infusa daging buah MD diberikan selama 4 minggu. Kerusakan testis diamatidengan pewarnaan hematoksilin eosin dengan menggunakan diameter tubulus seminiferus dan kriteria Johnsonuntuk menentukan tingkat spermatogenesis. Penghitungan jumlah sperma dilakukan pada cairan sperma dariductus defferens dengan pewarnaan Giemsa.Hasil Penelitian: Kelompok yang mendapat infusa daging buah PM memiliki tingkat spermatogenesis, diametertubulus seminiferus, jumlah sperma yang lebih tinggi (p < 0,05).Kesimpulan: Rebusan daging buah Mahkota dewa (Phaleria macrocarpa (scheff.) boerl.) memperbaiki kerusakantestis dan jumlah sperma pada tikus diabetik yang diinduksi alloksan (Sains Medika, 4(2):115-123).
Role of Adiponectin in Atherosclerosis Sulistyoningrum, Evy; Susilowati, Rina
Jurnal Anatomi Indonesia Vol 1, No 2 (2007)
Publisher : Jurnal Anatomi Indonesia

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Abstract

Role of Adiponectin in Atherosclerosis
PERUBAHAN SELULER DAN MOLEKULER PADA NEFROPATI DIABETIK Sulistyoningrum, Evy
MANDALA of Health Vol 7, No 1 (2014): Mandala Of Health
Publisher : Jurusan Kedokteran FK Unsoed

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Diabetic nehropathy is the most prevalent complication of diabetes mellitus. Hiperglycemia in diabetes mellitus activates several pathways leading to PKC activation. This activation then induce molecular changes involving many growth factors and cytokines. Various growth factors and cytokines further induce extracellular matrix accumulation and mesangial cell expansion leading to glomerular hypertrophy and sclerosis. This changes affects renal function in filtering albumin and further damage can cause abnormal renal function and lead to renal failure
TINJAUAN HISTOLOGIS PEMBULUH DARAH TIKUS PUTIH (Rattus norvegicus) DIABETES YANG DIBERI REBUSAN DAGING BUAH MAHKOTA DEWA (Phaleria macrocarpha (Scheff.) Boerl.) Sulistyoningrum, Evy; Kautsari, Septina; Susatyo, Priyo
MANDALA of Health Vol 4, No 2 (2010): Mandala Of Health
Publisher : Jurusan Kedokteran FK Unsoed

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Prevalence of Diabetes Mellitus among adult in Indonesia reached 4,1% in 1995 and projected to 6,5% in 2025. Complication of diabetes mellitus which come earlier and widely distributed yis atherosclerosis.Atherosklerosis can lead to other serious complications such as gangrene, stroke, cardiomiopathy, andcoronary artery diseases. This research was aimed to know the effect of Mahkota dewa (Phaleriamacrocarpha (Scheff.) Boerl.) boiled water to regeneration of aorta’s atherosclerosis post diabetic inductionwith alloxan. The research was conduct in four weeks to 25male albino rats (Rattus norvegicus) 3 monthaged, 120-180 grama weighed. The subjects was divided into five groups:positive control, negative control,A, B and C. A, B and C groups was treated Mahkota dewa boiled water with 4,5%, 9%, and 13,5%concentration respectively. The drug was administered orally. III, IV, and V groups are injected by alloxan toinduce diabetes. Atherosclerosis frequency was counted from histological section stained with Haematoxylineosin.Statistical analysis using Chi square analysis showed that Mahkota Dewa boiled water significantlyreduced atherosclerosis frequency in aorta. It can be concluded that Mahkota dewa (P.macrocarpha(Scheff.) Boerl.) regenerates atherosclerosis injury in diabetic blood vessel.
TINJAUAN MOLEKULAR DAN ASPEK KLINIS RESISTENSI INSULIN Sulistyoningrum, Evy
MANDALA of Health Vol 4, No 2 (2010): Mandala Of Health
Publisher : Jurusan Kedokteran FK Unsoed

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Abstract

Insulin resistance is defined by an unresponsive condition in the target organ that normallyresponded to insulin hormone. Insulin resistance play a major role in many serious health problem such asmetabolic syndrome, type II diabetes, polycistic ovarii syndrome, cancer and cardiovascular diseases. Themechanism of insulin resistance is still unclearly understood. Researchers postulated two mechanism ofinsulin resistance, based on inflammatory theory and obesity theory. These two causes of insulin resistancehave different mechanism of action. Clinical diagnosis of insulin resistance can be performed by clampmethod and minimal model of frequency sampled intravenous glucose tolerance test (FSIVGTT) but thesegold standard diagnosis are very invasive. Non-invasive methods for diagnosing insulin resistance can beperformed by using various formulas derived from base examination in glucose and insulin plasma level.One of the formulas which have high sensitivity and specificity is HOMA-IR index (Homeostasis ModelAssessment-Insulin Resistance). Different characteristic of population required different cut-off fordetermining insulin resistant condition.
PENGARUH PEMBERIAN SUSPENSI MENIRAN (Phyllanthus niruri L.) TERHADAP KERUSAKAN HEPAR TIKUS PUTIH YANG DIINDUKSI ANTITUBERKULOSIS RIFAMPISIN DAN ISONIAZID Sulistyoningrum, Evy; Pribadi, Fajar Wahyu
MANDALA of Health Vol 4, No 1 (2010): Mandala Of Health
Publisher : Jurusan Kedokteran FK Unsoed

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Abstract

Tuberculosis treatment required at least two antituberculosis drugs (ATDs) and long term course.Isoniazid (INH) and Rifampicin are the two most active ATDs and were used in whole course of treatment.INH and Rifampicin combination increased risk of hepatotoxixity. Meniran (Phyllanthus niruri L.) containsphyllanthin, active subtance that is believed to have hepatoprotective activity. The aim of this study was toknow the effect of meniran suspension on AST/ALT blood levels and histopathological findings afterinduction of Rifampicin and INH. Twenty five male albino rats (Rattus norvegicus) wistar strain aged twomonths and weighed 150-200 grams were divided into five groups of five each. Positive control (A) wastreated with aquadest, negative control (B) was treated with Rifampicin and INH; one dose meniran (I) waspre-treated with 16,2 mg meniran before ATDs; two dose meniran (II) was pre-treated with 32,4 mg meniranbefore ATDs, three dose meniran (III) was pre-treated with 48,6 mg meniran before ATDs. The drugs wereadministered orally for 28 days. Blood samples for ALT/AST levels and histopathology sample were taken atthe end of study. One way ANOVA, post hoc and linear regression were used for data analysis. There wassignificant mean difference for ALT levels (p=0,000) but not for AST level (p> 0,05). Increasing dose ofmeniran decreased serum level of ALT (r=-0,539). Vacuolar degeneration, necrosis and portal triadleucocytes infiltration were most common in negative control groups, while these changes were reduced inmeniran-treated groups. We can conclude that meniran pretreatment reduces INH-rifampicin-inducedhepatotoxicity.
Mahkota Dewa Mesocarp Infusion Improved Testicular Damage and Sperm Count In Diabetic Rat Sulistyoningrum, Evy; Setiawati, Setiawati; Nindyastuti, Herlina; Putra, Adrian Nugraha
Sains Medika Vol 4, No 2 (2012): Juli-Desember 2012
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

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Abstract

Background: Global prevalence of Diabetes Mellitus (DM) is predicted to be increasing with worldwide population increase, longer life expectancy, urbanization, change in lifestyle and higher incidence of obesity. Diabetes Mellitus which is characterized by increasing level of blood glucose can induce increasing activity of ROS (Reactive Oxygen Species) which can further lead to abnormality in male reproductive system, especially testis. Mahkota dewa (Phaleria macrocarpa (Scheff.) Boerl.=PM) contain flavonoid, alkaloid and saponin which can prevent further negative effect on ROS increase in male reproductive system. The aim of this study was to know effect of PM mesocarp infusion on testicular damage and sperm count in alloxan induced diabetic rat.Design and Method: The design of this study was post test only with control group design. Rats in this study were divided into 4 groups (6 rats each group): control group, diabetic group, diabetic group treated with PM mesocarp infusion (9% and 13.5%). Diabetes was induced by alloxan 50 mg/BW single dose injection intramuscularly. PM mesocarp’s infusion was given in 4 weeks. Testicular damage was observed in hematoxylin-eosin stain using Johnsen criteria for spermatogenesis level and tubulus seminiferous’ diameter. Sperm count was performed on the semen taken from vas defferens using Giemsa stain.Result: PM mesocarp infusion group has higher spermatogenic level (p < 0,05), higher tubulus seminiferus diameter and sperm count (p < 0,05) than diabetic group.Conclusion: Mahkota dewa mesocarp infusion improved testicular damage and sperm count in alloxan-induced diabetic rat (Sains Medika, 4(2):115-123).
Kafein Meningkatkan Ketebalan Tunika Muskularis Eksterna Rectum Embrio Gallus gallus Strain ISA Brown Arjadi, Fitranto; Sulistyoningrum, Evy
Prosiding 2nd Seminar Nasional IPTEK Terapan (SENIT) 2017 Vol 2, No 1 (2017): Mei 2017
Publisher : Politeknik Harapan Bersama Tegal

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Abstract

Kafein dipergunakan secara luas dalam konsumsi sehari-hari dan di bidang pengobatan. Kafein meningkatkan kadar katekolamin, merangsang pertumbuhan dan proliferasi otot polos dan dapat merangsang sistem saraf simpatis. Tujuan penelitian adalah mengetahui pengaruh pemberian kafein terhadap ketebalan tunika muskulartis eksterna rectum embrio Gallus-gallus strain ISA brown. Metode penelitian adlah eksperimental dengan menggunakan tigapuluh butir telur Gallus-gallus strain ISA brown fertil dengan berat 60-70 gram dikelompokkan dalam 2 kelompok,  15 butir dalam perlakuan “caff” yaitu diberi kafein 0,1 mg/ml setiap hari sejak hari ke-4 masa inkubasi sampai hari ke-8 inkubasi dan  kelompok kontrol mendapat aquabidestilasi dalamn waktu yang sama. Hari ke-19 inkubasi, hewan coba dibedah dan diambil rectum, diukur ketebalan tunika musculartis eksterna (stratum sirkuler dan stratum longitudinal) dibawah mikroskop. Hasil penelitian menunjukkan rerata ketebalan tunika muskularis rektum adalah 54,5313 µm (stratum longitudinal,“caff”)  dibanding 42,0833 µm (stratum longitudinal, “kontrol”) dan  74,2969 µm (stratum  sirkular “caff”) dibanding  72,7083 µm  (stratum sirkular, “kontrol”) tetapi tidak ada perbedaan yang signifikan antara kelompok perlakuan dengan ketebalan tunika muskularis rektum stratum longitudinal (t=0,442) dan stratum sirkular (t=0,135) secara statistik. Kesimpulan terdapat peningkatan ketebalan tunika muskularis eksterna rektum embrio Gallus-gallus strain ISA brown setelah pemberian kafein walaupun  secara statistik tidak signifikan.
D-galactose-induced animal model of male reproductive aging Sulistyoningrum, Evy
Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 8, No 1, (2017)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

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Aging is a complex biological process involving molecular, cellular and organic changes. Aging process is not merely caused by chronological age but it can be accelerated by environmental factors contributes to oxidative stress. Aging in male reproductive system is characterized by many conditions such as terticular atrophy, decreases testicular function of spermatogenesis, decreased testicular function of testosteron production which can lead to a serious clinical condition, infertility. Management of male infertility with excellent outcome is still a challenge and therefore new strategies need to be examined. Because of difficulties and ethical issues in human research, animal models of male reproductive aging are useful research tools for this purpose. Rodent models of male reproductive aging are the first choice because similarity of metabolism system and short lifespan. D-galactose animal model for aging seems to be closer to human aging of male reproductive system. It is an advantageous model for testing potencies of pharmacological agent and natural compounds on aging.
Pengaruh propolis terhadap profil lipid plasma tikus model hiperkolesterolemia Diah, Krisnansari; Hapsari, Ariadne Tiara; Sulistyoningrum, Evy; Prastowo, Agus
Jurnal Gizi Klinik Indonesia Vol 8, No 3 (2012): Januari
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, Fakultas Kedokteran UGM

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Background: Nowadays, cardiovascular disease caused by hypercholesterolemia has become the main cause of death. Propolis has been used widely to reduce plasma cholesterol levels.Objective: The aims of this research was to study the effect of propolis on lipid profile of hypercholesterolemic Sprague Dawley rats.Method: This was an experimental study with pre-post test. Twenty four (24) male Sprague Dawley rats aged 12-16 week old, weighing 125-200 g were allocated into 4 groups. Group I received standard meal + aquadest-gavage; group II received high cholesterol meal + PTU 0,01 + aquadest gavage; group III received high cholesterol meal + PTU 0,01 + 0,027 g propolis gavage; group IV received high cholesterol meal + PTU 0,01 + 0,054 g propolis gavage. Total cholesterol, triglycerides, HDL cholesterol and LDL cholesterol levels before and after treatment were measured. The data were then analyzed with One Way Anova.Results: The study showed that there were no significant differences in changes of body weight. There were significant differences in total cholesterol levels between all groups of treatment. Triglyceride levels were significantly different among all groups, except between group I and IV. Furthermore, the HDL cholesterol levels of group I vs III and group I vs IV were significantly different. However, there were no differences found in LDL cholesterol levels among all groups of treatment.Conclusion: Provision of 0,027 g and 0,054 g propolis improve lipid profile (total cholesterol, triglyceride and HDL cholesterol levels) of hypercholesterolemic rats.