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Journal : Jurnal Ilmu Pertanian

Effects of Plant Growth Promoting Rhizobacteria (PGPR) on Growth and Yield of Shallot in Sandy Coastal Land Tuhuteru, Sumiyati; Sulistyaningsih, Endang; Wibowo, Arif
Jurnal Ilmu Pertanian Vol 1, No 3 (2016): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.16349

Abstract

The marginal sandy coastal land should be utilized to maintain production level of shallot. But for increasing the productivity of sandy coastal land, in the shallot cultivation should be applied biological fertilizers, such as Plant Growth Promoting Rhizobacteria (PGPR). The purpose of this research was to obtain the most effective isolate of PGPR (Plant Growth Promoting Rhizobacteria) to the growth of three shallot cultivars in the sandy coastal land. The research had been conducted in August-November 2015 at Samas sandy coastal land, Bantul. The Factorial treatments were assigned in Randomized Complete Block Design with three blocks as replications. The first factor was shallot cultivars consisted of Crok, Tiron and Tuk-tuk, while the second factor was the PGPR suspension, i.e. control (without PGPR), PGPR isolates BP25.2 (Bacillus methylotrophicus), BP25.6 (Bacillus amyloliquofaciens), BP25.7 (Bacillus subtilis), BrSM 4 (Burkholderiacepacia), and BrSG 5 (Burkholderiaseminalis). The data of growth and yield were analyzed using ANOVA with ? = 5%, then followed by DMRT ? 5% . The result showed that Tiron cultivar was able to grow in sandy coastal land, proven from its good germination, high number of bulb, high fresh weight, and dry weight of bulb. Meanwhile, BP25.2 isolate was able to increase seed growth simultaneously of Tiron cultivar and BrSM 4 isolate was able to increase the chlorophyll content of Tuk-tuk cultivars.
Callus Induction on True Shallot Seed Explant Using a Combination of BA and 2,4-D Dharmayanti, Krisna; Sulistyaningsih, Endang; Wulandari, Rani Agustina
Jurnal Ilmu Pertanian Vol 2, No 3 (2017): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.26276

Abstract

BA and 2,4-D combination were commonly used for in vitro culture of Shallot (Allium cepa L. var agregatum 2n = 2x = 16) to induce callus, but there was no information for callus induction on shallot seed (TSS) explant. Callus could be utilized for in vitro selection and generating of genetic variation. The aims of the research was to identify the response of TSS (Trisula and Tuk Tuk) as explant  and to obtain the optimum combination of BA and 2,4-D (mg.L-1): (0?0, 2?1, 2?2, 2?3, and 2?4) in callus induction. The research had been carried out in the Tissue Culture Laboratory, Faculty of Agriculture, Universitas Gadjah Mada during the year 2015-2016. Factorial treatments of variety and growth regulators were arranged in Completely Randomized Design with four replications. Data of percentage of germination, shoot height, root length, percentage of callus formation, callus weight, and chromosomes number of callus were recorded. The results showed that combination of 2 mg.L-1 BA + (1? 4) mg.L-1 2,4-D induced callus formation on TSS but inhibits shoots and roots growth. The best callus proliferation was at a concentration of 1 mg.L-1 2,4-D. Tetraploid callus chromosomes (2n = 4x = 32) was detected in Trisula grown in the 2 mg.L-1 BA + 4 mg.L-1 2,4-D, but in the Tuk Tuk callus did not detected the changing of chromosomes number.
The Effect of Drying and Storage on the Quality of Shallot (Allium cepa L. Aggregatum group) Bulbs Sri Lestari, Rohimah Handayani; Sulistyaningsih, Endang; Purwantoro, Aziz
Jurnal Ilmu Pertanian Vol 3, No 3 (2018): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.34203

Abstract

Post-harvest handling in shallot such as drying of bulbs can influence its quality during and after storage. The objective of this study was to determine the impact of drying and storage treatment on the quality of shallot bulbs during 12 weeks of storage . The study was carried out in Samiran hamlet, Parangtritis village, Bantul district, Special Region of Yogyakarta and Crop Science Laboratory of the Faculty of Agriculture, Universitas Gadjah Mada, from June until November 2016. It was arranged in factorial randomized complete block design (RCBD) and consisted of two factors. The first was the drying treatments: drying the bulbs on the field and on woven bamboo nets both plastic covered and uncovered. The second was the storage treatments: storing the bulbs in the farmer?s warehouse (31,030C±0,04 and RH of 60,50%±0,28), in air-conditioned room (22,40oC± 0,02 and RH of 61,60%±0,09), and at room temperature (30,47oC±0,03 and RH of 60,50%±0,12). Each treatment combination was replicated three times as blocks. The results showed that water content of all treatments were changing followed by fluctuating of the total soluble solid throughout the storage period while bulb firmness tended to decrease. Bulbs which were stored in air-conditioned rooms showed the highest percentage of sprouted bulbs, vigor index and germination rate than other treatments. Meanwhile, drying treatment did not give significant influence.          
Various Shallot Seed Treatments with Trichoderma to Increase Growth and Yield on Sandy Coastal Darsan, Stefany; Sulistyaningsih, Endang; Wibowo, Arif
Jurnal Ilmu Pertanian Vol 1, No 3 (2016): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.12564

Abstract

Sandy coastal area is a poor land, with minimal nutrients and low ability to store water and nutrition. Therefore, plant is difficult to grow well, consequently the treatments for improving sandy coastal is required before planting to support their growth. Application of Trichoderma as a plant-growth promoting and controlling pathogens had been known. Shallot seed treatment by Trichoderma agents was intended to improve and support plant growth and yield in the sandy coastal land. The aim of experiment was to determine the most effective treatment to improve growth and yield of shallot on sandy coastal land. The experiment had been carried out in Yogyakarta during August - November 2015. The factorial treatments of cultivar and Trichoderma applications were arranged in Completely Randomized Design with three replications. The shallot cultivars consisted of Tiron, Crok, and Biru, while Trichoderma application consisted of control (no treatment), sprayed with Trichoderma, soaked in Trichoderma, matricontioning media i.e. rice husk charcoal and brick in combination with Trichoderma. The collected data related to plant growth and yield. The result showed that seed treatment methods with Trichoderma increased the plant height, the leaf area index, extensive root, net assimilation rate (NAR), plant growth rate (PGR), Nitrate Reductase Activities (NRA), total chlorophyll, and fresh bulb weight. The best technical treatment for cultivar Tiron was soaked with Trichoderma, and Crok was sprayed with Trichoderma, while Biru cultivar was using brick in combination with Trichoderma.
In Vitro Induced Resistance of Fusarium Wilt Disease (Fusarium oxysporum f.sp. cepae) by Salicylic Acid in Shallot CV ‘Bima Brebes’ Khotimah, Khusnul; Sulistyaningsih, Endang; Wibowo, Arif
Jurnal Ilmu Pertanian Vol 2, No 1 (2017): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.12840

Abstract

Fusarium wilt caused by Fusarium oxysporum f.sp.cepae (Foc) is one of the most important diseases of onion in Indonesia. Induced resistance was one of the effective techniques to acquire resistance to fusarium wilt in shallot. Salicylic acid (SA) had been inferred to be in endogenous signal in the systemic acquired resistance response of plants. This research aimed to study the effect of exogenously added SA to resistance in shallot callus cv ?Bima Brebes? to fusarium wilt disease in vitro; and to determine the effective concentration of SA to induce resistance. A group of shallot callus was grown in MS medium containing varying SA concentration (0, 15, 20 and 25 ppm) in vitro for 2 weeks. Then, the callus were treated with toxin of Fusarium oxysporum, namely fusaric acid, to have observe the resistance response. In vitro selection was done twice in different fusaric acid concentration. Application of exogenous SA at all concentration did not suppres time of appearance of disease symptom yet. Toxic symptom in the callus was shown by the browning or blackening (off) of callus. Salicylic acid at concentration of 20 ppm effectively reduced the toxic symptom up to 16.66% and supported callus regeneration better than the concentration of 15 ppm and 25 ppm. The number of resistant callus regenerated was 66.67% at pretreatment of 20 ppm of SA.
Effect of Cropping Pattern and Fertilizer Dose Applied in Raised-Bed on The Growth and Yield of Rice (Oryza sativa L.) in Sunken-Bed of The Surjan Rice Field Suryaningndari, Damar; Indradewa, Didik; Kurniasih, Budiastuti; Sulistyaningsih, Endang
Jurnal Ilmu Pertanian Vol 3, No 2 (2018): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.31420

Abstract

Surjan system (alternating bed system) is an agricultural system that combines dry and wet bedding system. It integrates food crop culture in the sunken-bed and annual crops in the raised-bed of the rainfed rice field. In Bantul, farmers commonly apply Surjan system in rice field by growing shallot (Allium cepa L. aggregatum group) and chili (Capsicum annuum L.) in the raised-bed, whereas no crop is grown in the sunken-bed. This present experiment evaluated the utilization of the sunken-bed for rice cultivation by utilizing fertilizer runoff from the raised-bed. Rice plants in the sunken-bed were not fertilized due to the expectation that it could utilize the fertilizer runoff from the raised-bed. The cropping pattern and the fertilizer dose in the raised-bed are suspected to affect the growth and yield of rice in the sunken-bed. The purpose of this study was to determine the effect of cropping pattern and fertilizer dose applied in raised-bed on the growth and yield of rice in sunken-bed of the Surjan rice field. This study were laid out in a split plot design with three replication. The main plot consists of two cropping pattern, namely shallot monoculture and intercropping shallot with chili. The fertilizer dose applied in raised-bed, namely 100% farmer's habit; 50% farmer?s habit; and 25% farmers' habit, occupying the sub plot. Dose of fertilizer applied in riased- bed according to the farmer's habit in research locatin is 622 kg NPK/ha (16-16-16) ; 228 kg ZA/ha and 76 kg KCl/ha. The results showed that there was no interaction between the cropping pattern and the fertilizer dose applied in the raised-bed on the growth and yield of rice in the sunken-bed. Compared with shallot monoculture, intercropping shallot with chili in raised-bed decreased the growth of rice in the sunken-bed. Compared with the 100% fertilizer dosage of farmer's habits, the fertilizer dose of 50% of the farmer's habits in the raised-bed increased the growth of rice in the sunken-bed. Cropping pattern and fertilizer dose in the raised-bed did not significantly affect the rice yield grown in the sunken-bed of the Surjan rice field.
Effects of Pyraclostrobin on Growth and Yield of Curly Red Chili (Capsicum Annum L.) Hardiansyah, Arizal Nur; Sulistyaningsih, Endang; Putra, Eka Tarwaca Susila
Jurnal Ilmu Pertanian Vol 2, No 1 (2017): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.12841

Abstract

Curly red chili was one of vegetable commodities in Indonesia used for seasoning of home cuisine, food industry, and pharmaceutical industry. Curly red chili cultivation needed fungicide to prevent fungal disease. Pyraclostrobin was a fungicide which could overcome fungal attack and improve plant growth. This research was conducted to study the effectiveness of pyraclostrobin application on vegetative growth of curly red chili plant. Research had been done in farmer?s land in Kemiriombo Village, Dukun Sub District, Muntilan District, Magelang Regency from December 2013 to June 2014. The treatments were assigned in the Randomized Complete Block Design with three replications. The treatments consisted of P1: pyraclostrobin doses 1.5 kg ha-1, 30 and 90 days after planting (dap), P2: pyraclostrobin 1.5 kg ha-1 at 30, 60, and 90 dap, P3: pyraclostrobin 1.5 kg ha-1, at 15, 30, 60, and 90 dap, P4: pyraclostrobin 3 kg ha-1, at 30 and 90 dap, P5: pyraclostrobin 3 kg ha-1, at 30, 60, and 90 dap, P6: pyraclostrobin 3 kg ha-1, at 15, 30, 60, and 90 dap, and P0: control (no treatment). Data were analyzed by orthogonal contrast test with ?=5%. The result indicated significant different in the dry weights of root, stem, leaf, and total yield at 12 weeks after planting; number of flower at 9-11 week after planting, 14 week after planting, and 18-21 week after planting; and number of fruits at 10-12 week after planting, 15, and 16 week after planting. The application of pyraclostrobin at all dosages could increase IAA content. The numbers of flower and fruit were influenced by the increasing of IAA content in plant tissue, but did not affect the yield.
The Effect of Various Weedy Periods on Growth and Yield of Soybean in Agroforestry System with Kayu Putih Nugroho, Joko Budi Santoso; Sulistyaningsih, Endang; Suryanto, Priyono
Jurnal Ilmu Pertanian Vol 2, No 1 (2017): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.17700

Abstract

This study was aimed to detect the effect of various treatment periods of weedy on the growth and yield of soybean; and to determine the most appropriate time periods of weedy for soybean in agroforestry systems with kayu putih. The experiment had been conducted in Menggoran, BDH Playen, KPH Yogyakarta, Gunungkidul Regency, Special Province of Yogyakarta from February 28 to May 9, 2015. Randomized Complete Block Design (RCBD) with three blocks as replications waas applied  in this experiment. The treatments were weedy periods on 0, 14, 28, 42, and 56 days after planting (dap) and weed-free period on 0, 14, 28, 42, and 56 dap. The results showed that there were significant differences in the soil moisture content, root surface area, root length, chlorophyll content, root dry weight, shoot dry weight, dry weight of soybean seeds, and weed dry weight. The highest seed weight per hectare was found in weed-free until harvest treatment but it was not significantly different from weedy periods after 56 dap and weed-free after 14 dap. The effective periods of weedy time for soybean in agroforestry systems with kayu putih began at 28 dap - 56 dap.
Shallot Growth and Yields Based on Ammonium:Nitrate Ratio on Coastal Sandy Soil Safitri, Wiji; Sulistyaningsih, Endang; Purwanto, Benito Heru; Harper, Stephen
Jurnal Ilmu Pertanian Vol 2, No 2 (2017): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.18333

Abstract

Nitrogen was a key component for increasing yield and quality of vegetables like shallots. The growth and development of plants were influenced by nitrogen form. Common plants preferred nitrate for growth, but the enormity preference varies within plant species and other environmental factors. The purpose of this experiment was to study the effect of ammonium:nitrate ratio in sandy soil to growth and yield of shallot (Allium cepa L. Aggregatum group). The experiment had been conducted in August-October 2015 in the sandy land on Samas Beach, Bantul, Yogyakarta, Indonesia. The research was arranged in a Split-Plot design. The main plot was the source of nitrogen (NH4+; NO3-; NH4+: NO3- 1:1; NH4+: NO3- 1:2; and NH4+: NO3- 2:1) and the subplot was cultivars (Crok Kuning, Tiron, and Bima Brebes). Each subplot covered an area of 2.5 m² (2.5 m x 1 m) with three blocks as replications. Shallot bulbs were planted in sixth-row, spacing 20 cm between rows and 15 cm within rows. The dose was applied according to the recommendation of BPTP (urea 200 kg ha-1, ZA 250 kg ha-1, SP-36 150 kg ha-1, and KCl 150 kg ha-1) 144.5 kg N ha-1. Ammonium:nitrate ratio influenced shallot growth in sandy soil through number of leaves at maximum vegetative phase and its leaf area. The NH4+: NO3- 1:2 ratio gave the best result in dry weight of leaves following bulb dry weight than other ratio. Nevertheless, ammonium:nitrate ratio had no significant influence on bulb dry weight (ton ha-1).
The Growth and Flowering of Potted Chrysanthemum (Chrysanthemum morifolium Ramat) on Types of Organic Media and Watering Frequent Rahmawati, Ika; Sulistyaningsih, Endang
Jurnal Ilmu Pertanian Vol 4, No 2 (2019): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.42163

Abstract

The soils in Samigaluh are mostly dominated by clay and used by farmer for crops production. The growing media for potted chrysanthemum requires additional organic media that could be mixed with the organic material to improve chemical and physical soil properties. Furthermore, the research location is a region with little water in dry season. The research aimed to determine the growth and flower yield response of Avanthe agrihorti to the different organic material mixed with clay and watering frequency. The research was arranged in a split plot design with 3 replications, conducted at an altitude of 462 m above sea level in the village of Gerbosari, Samigaluh, from March to June 2018. The main plot was watering frequency, i.e. every day, every three days and every five days. The subplot was types of media, i.e. clay + manure, clay + manure + cocopeat, clay + manure +rice husk, and clay + manure + rice husk charcoal. Data on the growth and yield of chrysanthemum plants were observed and statistically analyzed with ANOVA and continued with DMRT test at 5%. The results showed that the types of media and watering frequency did not significantly give different effect on most of the observed variables. However, Avanthe Agrihorti planted on clay + manure + cocopeat at all watering frequencies showed better growth and yield of flowers than those planted on other media. The efficient watering frequency for Avanthe Agrihorti was every three days. This study provides information for farmers on an alternative method to prepare the best media for the cultivation of potted chrysanthemum on the soil clay.