Articles

Development of Sequence-Based Microsatellite Marker for Phalaenopsis Orchid FATIMAH, .; SUKMA, DEWI
HAYATI Journal of Biosciences Vol 18, No 2 (2011): June 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (88.455 KB)

Abstract

Phalaenopsis is one of the most interesting genera of orchids due to the members are often used as parents to produce hybrids. The establishment and development of highly reliable and discriminatory methods for identifying species and cultivars has become increasingly more important to plant breeders and members of the nursery industry. The aim of this research was to develop sequence-based microsatellite (eSSR) markers for the Phalaenopsis orchid designed from the sequence of GenBank NCBI. Seventeen primers were designed and thirteen primers pairs could amplify the DNA giving the expected PCR product with polymorphism. A total of 51 alleles, with an average of 3 alleles per locus and polymorphism information content (PIC) values at 0.674, were detected at the 16 SSR loci. Therefore, these markers could be used for identification of the Phalaenopsis orchid used in this study. Genetic similarity and principle coordinate analysis identified five major groups of Phalaenopsis sp. the first group consisted of P. amabilis, P. fuscata, P. javanica, and P. zebrine. The second group consisted of P. amabilis, P. amboinensis, P. bellina, P. floresens, and P. mannii. The third group consisted of P. bellina, P. cornucervi, P. cornucervi, P. violaceae sumatra, P. modesta. The forth group consisted of P. cornucervi and P. lueddemanniana, and the fifth group was P. amboinensis.
Induksi Ginogenesis melalui Kultur Multi Ovule Slice dan Kultur Ovary Slice Dianthus chinensis Kartikaningrum, Suskandari; Purwito, Agus; Wattimena, Gustaaf Adolf; Marwoto, Budi; Sukma, Dewi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 41, No 1 (2013): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (381.553 KB)

Abstract

Callus induction was studied in five genotypes of Dianthus chinensis using 2.4 D and NAA. Calluses can be obtainedfrom unfertilized ovule culture and ovary culture. The aim of the research was to study gynogenic potential and responseof Dianthus chinensis through ovule slice and ovary slice culture for obtaining haploid plants. Five genotypes of Dianthuschinensis and five media were used in ovule slice culture and two genotypes and three medium were used in ovary culture.Flower buds in the 7th stage were incubated for the purpose of dark pre-treatment at 4 oC for one day. Ovules and ovaries wereisolated and cultured in induction medium. Cultures were incubated for the purpose of dark pre-treatment at 4 oC for seven days, followed by 25 oC light incubation. The result showed that 2.4D was better than NAA in inducing callus. Percentage of regenerated calluses were produced in V11, V13 and V15 genotypes in M7 medium (MS + 2 mg L-1 2.4D + 1 mg L-1 BAP + 30 g L-1 sucrose and M10 medium (MS + 1 mg L-1 2.4D + 1 mg L-1 BAP + 20 g L-1 sucrose). All calluses originated from ovule and ovary cultures flowered prematurely. Double haploid (V11-34) were obtained from ovule slice culture based on PER (peroksidase) and EST (esterase) isoenzym marker.Keywords: ovule slice culture, ovary slice culture, callus, Dianthus sp., haploid
Aktivitas Kitinase dan Peroksidase dari Ekstrak Kasar Protein Asal Kalus dan Berbagai Jaringan Tanaman Trichosanthes cucumerina var. anguina Sukma, Dewi; Poerwanto, Roedhy; Sudarsono, ,; Khumaida, Nurul; Artika, I Made; Wiyono, Suryo
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 40, No 3 (2012): JURNAL AGONOMI INDONESIA
Publisher : Bogor Agricultural University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (296.26 KB)

Abstract

Chitinase and peroxydase are important bioactive proteins or are specific enzymes that are related to plant resistance to pathogens. The aims of the research were to analyze the chitinase and peroxidase activities of crude protein extract from calli, stem, leaves and roots of T. cucumerinavar. anguina. In the first experiment, chitinase and peroxydase activities were analyzed from in vitro calli, leaves and roots obtained from 2-month-old of field grown plants. The media for calli induction were Murashige and Skoog medium with addition of 1 µM NAA + 1 µM BA, 2 µM NAA + 2 µM BA, 3 µM NAA + 3 µM BA, or 4 µM NAA + 4 µM BA. In the second experiment, the chitinase and peroxydase activities from crude protein extract of roots, stems and leaves were analyzed. The extracts were from 3-week-old seedling (less than a month), 1-month and 2-month-old plants. The first and the second experiment results showed that crude protein extracts of plant roots from the field grown plants had the highest chitinase and peroxidase activities. Stem of field grown plants had the similar level of chitinase activities with the plant roots. Chitinase activities of in vitro calli were not significantly different from those of plant roots so that it could be used as an alternative for plant roots in studying chitinase from T. cucumerinavar. anguina. Chitinase activities in crude protein extracts of roots appeared constant whereas peroxidase tend to increase with plant age. Keywords: calli, enzyme activities, leaves, roots, stem
Variasi Genetik Mutan Anggrek Spathoglottis plicataBlume. Berdasarkan Marker ISSR Romeida, Atra; Sutjahjo, Surjono Hadi; Purwito, Agus; Sukma, Dewi; Rustikawati, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 40, No 3 (2012): JURNAL AGONOMI INDONESIA
Publisher : Bogor Agricultural University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (390.572 KB)

Abstract

Mutants of Spathoglottis plicataBlume were obtained from plants treated with Gamma Irradiation at 30-100 Gray. The mutants showed variations in the flower morphology. The objective of this experiment was to identify genetic variations of orchids S. plicataand its mutants using inter-simple sequent repeat (ISSR) markers. The amplified product of 10 ISSR primers produced 360 bands and 71 ISSR of the loci (90.14%) were polymorphic. The coefficient of similarity and principal component analysis produced five major groups with similarity coefficient of 0.68. The goodness of fit correlation matrix value reached 0.91. Therefore the ISSR isa good marker for identification of S. plicatamutants.Keywords: gamma irradiation, ISSR marker, mutant, orchid
Aktivitas Kitinase dan Peroksidase dari Ekstrak Protein Daun, Akar, Kalus dan Tunas In Vitro Trichosanthes tricuspidata Lour. Sukma, Dewi; Poerwanto, Roedhy; Sudarsono, ,; Khumaida, Nurul; Wiyono, Suryo; Artika, I Made
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 1 (2008): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (188.991 KB)

Abstract

A number of Trichosanthes species has been reported as a source of bioactive protein associated with defense mechanisms such as chitinase. Chitinase and peroxidase of crude protein extracted from leaves, roots, in vitro calli and shoots of T. tricuspidata had been analysed. Calli were induced on MS medium containing combinations of 1 µM NAA + 1 µM BA  (K1), 2 µM NAA + 2 µM BA (K2), 3 µM NAA + 3 µM BA (K3), or 4 µM NAA + 4 µM BA (K4). Shoots were cultured in MS with 1 mg/l of BA, while leaves and roots were harvested from six-month old plants grown on the field. Results of the experiment suggested that K1-K4 medium could be used to induce calli although weight of calli from all medium composition  was not significantly different (0.19-0.31 g/explant/4 weeks). Calli from K1 medium had the highest of total crude protein content (3.24 mg/ml). The highest of chitinase activity was found in in vitro shoots (6.51 mM pNP/hour/mg protein) and the highest peroxidase activity was in the plant roots (0.25 ∆ 420/minute/mg protein).   Key words:  in vitro calli, shoots, crude protein, chitinase and peroxidase activities
Karakter Morfologi dan Kimia 18 Kultivar Pamelo (Citrus maxima (Burm.) Merr.) Berbiji dan Tanpa Biji Susanto, Slamet; Rahayu, Arifah; Sukma, Dewi; Dewi, Iswari S.
Jurnal Ilmu Pertanian Indonesia Vol 16, No 1 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Original Source | Check in Google Scholar | Full PDF (610.042 KB)

Abstract

Pamelo is one of the oranges species which have variety of form, size, colour and taste. Most of the pamelo cultivars with seeds, while part of it is seedless. The concumen prefer to chose seedles than with seed because they could consume more. The research proposed to know the morphologycal and chemical characteristics of pamelo with seed and seedless. Characteristication done in RGCI and port harvesting laboratory at IPB to the Pamelo come from Sumedang, Pati, Kudus, Magetan, Aceh and Pangkep (South Sulaewsi) in the period of April 2009 until July 2010. The result of research shown that several pamelo seedless cultivars have pyriform, while other with seed have spheroid form. A few fruit from pamelo seedless cultivars have sweet taste until less taste, with pH of its juice vary from 6.2-6.3, except Jawa cultivar which have pH 4 which its total tertiration acid is 0.47- 0.50 g/g, PTT 9.8- 11.0 ( 0brix), PTT/ATT 19,5-25,3. Vitamine C content 38-48.2 mg/100 g and narigin content from 118,3-1063,2 mg/ml, while pamelo with seed have taste sweet acid, with fruit juice pH 3.7 - 4.7, except "red bali 1", which have pH 6.0, ATT 0,35 - 0,59 g/g, PTT 8.7 - 11.3 ( 0brix), PTT/ATT 16.9 - 24.6, vita mine C content 28.6 - 43.8 and narigin content 55.2 - 461.2 mg/ml. Keywords: Pamelo, morphology characters, chemical characters, PTT/ATT, narigin. 
Karakterisasi Morfologi Anggrek Phalaenopsis spp. Spesies Asli Indonesia Fauziah, Ni'mah; Aziz, Sandra Arifin; Sukma, Dewi
Buletin Agrohorti Vol 2, No 1 (2014): Januari 2014
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Original Source | Check in Google Scholar | Full PDF (237.498 KB)

Abstract

Keberadaan anggrek Phalaenopsis spesies atau anggrek hutan sudah semakin menurun, sehingga perlu dilakukan upaya perbanyakan untuk melestarikannya. Upaya perbanyakan melalui persilangan buatan merupakan salah satu upaya untuk menghasilkan tanaman yang lebih baik dari tetuanya, namun sering mengalami kegagalan akibat incompatibitas tetua. Hal tersebut dapat dikurangi dengan melakukan karakterisasi untuk mengetahui kekerabatan antar tetua persilangan. Tujuan dari penelitian ini adalah untuk mempelajari karakter morfologi 14 aksesi anggrek Phalaenopsis spesies yaitu Phalaenopsis gigantea A (PGA), Phalaenopsis bellina (PB),? Phalaenopsis amabilis Halong (PAH), Phalaenopsis violacea (PV), Phalaenopsis doritis (PD), Phalaenopsis schilleriana (PS), Phalaenopsis modesta (PM), Phalaenopsis cornu-cervi (PC), Phalaenopsis pantherina (PP), Phalaenopsis tetraspis (PT), Phalaenopsis pulchra (PPA), Phalaenopsis amabilis Cidaun (PAC), Phalaenopsis amabilis Trenggalek (PAT) dan Phalaenopsis gigantea B (PGB). Hasil penelitian menunjukkan setiap anggota masing-masing aksesi pada Phalaenopsis spesies memiliki kekerabatan sangat dekat yaitu 100% kecuali Phalaenopsis tetraspis yang berkerabat 87% akibat perbedaan bentuk ujung daun. Berdasarkan klasifikasi daun aksesi yang berkerabat dekat adalah Phalaenopsis gigantea A dengan Phalaenopsis bellina (87%), Phalaenopsis amabilis Trenggalek dengan Phalaenopsis gigantea B (87%), Phalaenopsis violacea dengan Phalaenopsis pulchra (87%) dan Phalaenopsis amabilis Halong dengan Phalaenopsis pantherina (87%). Berdasarkan klasifikasi bunga aksesi yang memiliki kekerabatan dekat adalah Phalaenopsis violacea dengan Phalaenopsis modesta (78%). Berdasarkan klasifikasi daun dan bunga aksesi yang berkerabat dekat adalah Phalaenopsis violacea dengan Phalaenopsis modesta (70%).
Pengaruh Waktu dan Frekuensi Aplikasi Pupuk Daun Terhadap Pertumbuhan dan Pembungaan Anggrek Dendrobium ’Tong Chai Gold’ Sukma, Dewi; Setiawati, Ary
Jurnal Hortikultura Indonesia Vol 1, No 2 (2010): Jurnal Hortikultura Indonesia
Publisher : Perhimpunan Hortikultura Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (31.479 KB)

Abstract

Most of orchids grow slowly. Low fertilizer concentration with high frequency of fertilization can be the alternative to increase orchids growth. The objective of the research was to evaluate the effect of time and frequency of fertilizer application on Dendrobium ‘Tong Chai Gold’. The experiment was arranged in randomized of completely block design with two factor of treatment. The first factor is time of application and the second one is frequency of application of foliar fertilizer. The time applications in the morning about 6.30 to 7.30 o’clock, at mid day about 11.30 to 12.30 o’clock, and in the afternoon about 16.30 to 17.30 o’clock. The frequency of foliar fertilizer application were every three days or six days. The result of this experiment showed that time and frequency application of foliar fertilizer could not increased number of bulb, but increased young leaf length, and width. Fertilizer was applied in the morning combined with every three  days frequency of fertilizer tended to result  a better flowering of Dendrobium ‘Tong Chai Gold’.   Key words : Dendrobium, fertilizer, application time and  frequency, growth, flowering, 
Budi Daya Lisianthus (Eustoma grandiflorum (Raf.) Shinn.) di Mayungan, Bali Demas, Antony; Sukma, Dewi; Kartika, Juang Gema
Buletin Agrohorti Vol 4, No 3 (2016): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Original Source | Check in Google Scholar | Full PDF (401.246 KB)

Abstract

Tujuan dari penelitian ini adalah untuk meningkatkan pengetahuan dan keterampilan dasar yang berkaitan dengan pengalaman praktik kerja lapangan pada budi daya lisianthus (Eustoma grandiflorum (Raf) Shinn.). Penelitian dilaksanakan di Mayungan, Bali dari Februari - Juni 2009. Selama penelitian berlangsung, pekerjaan yang dilakukan sebagai buruh, pengawas asisten, dan asisten manajer. Beberapa data diperoleh melalui wawancara dan diskusi dengan pekerja dan manajer, selain itu juga pengamatan langsung di lapangan. Informasi yang diperoleh berkaitan dengan profil perusahaan, kondisi tanah, konstruksi penanaman, dan kendala pekerjaan. Fakta budi daya lisianthus, seperti pengembangan tanaman di pembibitan dan rumah kaca, karakteristik tanaman, metode irigasi, pupuk, persentase tanaman produktif, sebaran panen, dan vase life telah diamati. Lisianthus memiliki karakteristik yang unik sebagai tanaman bunga potong, selain itu bervariasi antar varietasnya. Metode budidayanya tidak hanya merawat tanaman muda pada plug produksi tetapi juga menumbuhkan lisianthus dalam rumah kaca produksi, hingga penanganan pascapanen. Terdapat kekurangan dalam produktivitas tanaman yang terlihat sangat rendah. Secara khusus pada produksi dan sistem pemasaran dalam rangka untuk bersaing dengan petani bunga potong berpengalaman dan terlatih.
Aktivitas Kitinase dan Peroksidase dari Ekstrak Protein Daun, Akar, Kalus dan Tunas In Vitro Trichosanthes tricuspidata Lour. Sukma, Dewi; Poerwanto, Roedhy; Sudarsono, ,; Khumaida, Nurul; Wiyono, Suryo; Artika, I Made
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 1 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

Show Abstract | Original Source | Check in Google Scholar

Abstract

A number of Trichosanthes species has been reported as a source of bioactive protein associated with defense mechanisms such as chitinase. Chitinase and peroxidase of crude protein extracted from leaves, roots, in vitro calli and shoots of T. tricuspidata had been analysed. Calli were induced on MS medium containing combinations of 1 ?M NAA + 1 ?M BA ?(K1), 2 ?M NAA + 2 ?M BA (K2), 3 ?M NAA + 3 ?M BA (K3), or 4 ?M NAA + 4 ?M BA (K4). Shoots were cultured in MS with 1 mg/l of BA, while leaves and roots were harvested from six-month old plants grown on the field. Results of the experiment suggested that K1-K4 medium could be used to induce calli although weight of calli from all medium composition ?was not significantly different (0.19-0.31 g/explant/4 weeks). Calli from K1 medium had the highest of total crude protein content (3.24 mg/ml). The highest of chitinase activity was found in in vitro shoots (6.51 mM pNP/hour/mg protein) and the highest peroxidase activity was in the plant roots (0.25 ? 420/minute/mg protein). ? Key words: ?in vitro calli, shoots, crude protein, chitinase and peroxidase activities
Co-Authors , Nazi, , , Rustikawati , Sudarsono ,, Giyanto . FATIMAH AGUS PURWITO Agus Rachmat Agus Sutanto Aji, Titistyas Gusti Alvianti Yaufa Desita, Alvianti Yaufa Arifah Rahayu Arrin Rosmala, Arrin Ary Setiawati Atra Romeida Azmi, Tubagus Kiki Kawakibi Budi Marwoto Catur Hermanto Demas, Antony Demas, Antony DINI NURDIANI Eka Martha Della Rahayu Elina, Juanita Elina, Juanita ELSJE T. TENDA, ELSJE T. Erick Raynalta, Erick Erna Sulistiana Fajar Pangestu, Fajar Fauziah, Ni'mah Fauziah, Ni'mah Firgiyanto, Refa Giyanto, dan Gustaaf Adolf Wattimena HAJRIAL ASWIDINNOOR Handini, Aline Sisi Hengky Novarianto I MADE ARTIKA Irawati Irawati Ismail Maskromo Iswari S. Dewi Juang Gema Kartika Kartiman, Roni Krishardianto, Adimas Krishardianto, Adimas M. Syukur Megawati, Gina MEITY A. TULALO, MEITY A. MUHAMAD SYUKUR Muhammad Syukur Noer, Ira Fauziah Noer, Ira Fauziah Nofia Hardarani NUR AJIJAH, NUR Nurul Khumaida Putri, Astrid Aditya Putri, Yane Riana Putri, Yane Riana rawati ,, rawati Ritonga, Arya Widura ROEDHY POERWANTO Rr. Sri Hartati Rubiyo Rubiyo Sandra A. Aziz SANDRA ARIFIN AZIZ Sandra Arifin Aziz, Sandra Satya Nugroho Sintho Wahyuning Ardie Slamet Susanto SUDARSONO SUDARSONO Sudarsono, dan sudarsono, dan SUKENDAH SUKENDAH, SUKENDAH SURJONO HADI SUTJAHJO Surjono Hadi Sutjahjo SURYO WIYONO Suskandari Kartikaningrum Swastika, Maria Syarifah Iis Aisyah Syarifah Iis Aisyah, Syarifah Syukur, dan Muhamad Syukur, dan Muhamad Tya Wulandari, Tya Ummu Kalsum Wahyu Arif Sudarsono Yafqori Ardigusa, Yafqori Yasmin, Zahra Fadhlia Yasmin, Zahra Fadhlia Yulianti, Yustia