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AKTIVITAS ANTIHIPERURIKEMIA EKSTRAK ETIL ASETAT DAN ETANOL BUAH SALAK VARIETAS BONGKOK (Salacca edulis Reinw.) PADA TIKUS GALUR WISTAR [Antihyperuricemic Activity of Ethyl Acetate and Ethanol Extracts of Snake Fruit var. Bongkok (Salacca edulis Reinw.) o Afrianti, Leni Herliani; Sukandar, Elin Yulinah; Adnyana, I Ketut; -, Slamet Ibrahim
Jurnal Teknologi Dan Industri Pangan Vol 22, No 1 (2011): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/3389

Abstract

AKTIVITAS ANTIHIPERURIKEMIA EKSTRAK ETIL ASETAT DAN ETANOL BUAH SALAK VARIETAS BONGKOK (Salacca edulis Reinw.) PADA TIKUS GALUR WISTAR [Antihyperuricemic Activity of Ethyl Acetate and Ethanol Extracts of Snake Fruit var. Bongkok (Salacca edulis Reinw.) on Wistar Rat] Leni Herliani Afrianti1)*, Elin Yulinah Sukandar2), I Ketut Adnyana2), dan Slamet Ibrahim3) 1) Departemen Teknologi Pangan Universitas Pasundan Bandung 2) Farmakologi-Farmasi Klinik, Sekolah Farmasi Institut Teknologi Bandung 3) Farmasi Kimia Analisis, Sekolah Farmasi Institut Teknologi Bandung   Diterima 07 Januari 2009 / Disetujui 21 Juni 2011 ABSTRACT   The aims of the study was to determine antihyperuricemic activity of ethyl acetate and ethanol extractsof snake fruit (Salacca edulis Reinw.) var. Bongkok on Wistar male rats. Wistar male rats administered with 100 and 200 mg/kg b.w ethyl acetat extract and 200 mg/kg b.w ethanol extract and simulationsly induced with potassium oxonate peritoneally and uric acid orally showed descreased uric acid serum level significantly as compared to control group at 6th and 7thhour (p < 0.05). Meanwhile ethanol extract at 100 mg/kg bw did not affect uric acid serum level significantly. Determination of uric acid level in urine of the rats, indicated that administration ofethanol extract at 200 mg/kg bw, orprobenecid as a standard at 45 mg/kg bw, increased excretion of urine uric acid level significantly as compared to control group at 7thhour (p < 0.05). Additionally, administration of ethyl acetate extract at 100 and 200 mg/kg bw did not show an increase of uric acid excretion in urine. Mechanism of action of the ethyl acetate extract and ethanol extract as an antihyperuricemic agent has been proposed by inhibition of xanthine oxidase activity wich decrease the synthesis of uric acid. Hence, the mechanism of action of antihyperuricemia of the ethanol extract was suggested to be an uricosuric i.e. increases the excretion of urine uric acid and xanthine oxidase inhibitory.   Keyword : Snake fruit var. Bongkok, ethyl acetate extract, ethanol extract, Antihyperuricemic, probenecid, Wistar male rat
SENYAWA ASAM 2- METILESTER-1-H-PIROL-4-KARBOKSILAT DALAM EKSTRAK ETIL ASETAT BUAH SALAK VARIETAS BONGKOK SEBAGAI ANTIOKSIDAN DAN ANTIHYPERURICEMIA Afrianti, Leni Herliani; Sukandar, Elin Yulinah; Ibrahim, Slamet; Adnyana, I Ketut
Jurnal Teknologi Dan Industri Pangan Vol 21, No 1 (2010): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/2464

Abstract

SENYAWA ASAM 2- METILESTER-1-H-PIROL-4-KARBOKSILAT DALAM EKSTRAK ETIL ASETAT BUAH SALAK VARIETAS BONGKOK SEBAGAI ANTIOKSIDAN DAN ANTIHYPERURICEMIA [Studies on 2-Methylester-1-H-Pyrolle-4-Carboxylic Acid Compound in Ethylacetate Extract of Snake Fruit Variety Bongkok as Antioxidant and Anthyperuricemic] Leni Herliani Afrianti 1)*, Elin Yulinah Sukandar 2), Slamet Ibrahim 3), dan I Ketut Adnyana 2). 1) Departemen Teknologi Pangan Universitas Pasundan, Bandung. 2) Farmakologi-Farmasi Klinik, Sekolah Farmasi Institut Teknologi Bandung. 3) Farmasi kimia Research Group, Sekolah Farmasi Institut Teknologi Bandung.   Diterima  1 Juli 2009 / Disetujui 20 Mei  2010 ABSTRACT   The aim of the study was to determine the antioxidant and antihyperuricemia activity of ethyl acetate extract of snake fruit (Salacca edulis Reinw.) var. Bongkok. The research methods used in this study comprised  of three stages. First stage, the isolation processes, consist ed of maceration, fractionation, and purification using several techniques of chromatography. The chemical structures of the isolated compounds were determined based on UV, IR, 1-D NMR, and 2-D NMR spectral data. The ethyl acetate extract of snake fruit var. Bongkok isolated was a new compound 2-methylester-1-H-pyrolle-4- carboxylic acid. In the second stage the antioxidant activity of the extract and the isolated compounds were measured by 1,1 diphenol  (DPPH) method. The antioxidant activity of the extracts and the isolated compounds were expressed as IC50, The ethyl acetate extracts at concentrations of 0.2, 2, 20, 200, 400, and 2000 µg/mL showed inhibition of 9.67, 4.47, 41.89, 96.06, 82.54, and 90.60 % respectively, with an IC50 of 1.6 µg/mL. Ascorbic acid standards at the same concentration range showed an  IC50 of 0.54 µg/mL. Meanwhile, at the same concentrations the 2-methylester-1-H-pyrolle-4-carboxylic acid showed free radical inhibition of 17.48, 21.48, 18.14, 31.87, and 62.34 % respectively, with an  IC50 of 3.27  µg/mL. During the third stage,  the antihyperuricemic properties of the extracts and the isolated compound were examinated in vitro using inhibition of xanthin oxidase method. The ethyl acetate extracts at concentrations of 0.01, 0.02, 0.2, 2, and 2000  µg/mL showed xanthin oxidase inhibition of 49.24, 49.58, 50.28 and 52.26 % respectively, with an  IC50 of 24.75 µg/mL. At the same concentrations, the 2-methylester-1-H-pyrolle-4- carboxylic acid, showed xanthin oxidase inhibition of 27.7, 30.5, 37.3, 50.27 and 50.55 % respectively, with an IC50 of 48.86 µg/mL.  Allopurinol as a standard drug showed an IC50 of 0.92 µg/mL.   Key word : 2-methylester-1-H-pyrolle-4- carboxylic acid compound, ethyl acetate extract of snake fruit var. Bongkok, antioxidant activity, antihyperuricemia, IC50.
Pengaruh Pemberian Ekstrak Air Daun Jati Belanda (Guazuma Ulmifolia Lamk.) terhadap Kadar Lipid Darah pada Tikus Jantan Sukandar, Elin Yulinah; ., Elfahmi; ., Nurdewi
Jurnal Kedokteran Maranatha Vol 8, No 2 (2009)
Publisher : Universitas Kristen Maranatha

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Jati belanda (Guazumaulmifolia Lamk) has traditionally been used as a slimming agent. This study attempted to determine the effect of water extract of jati belanda on the blood&nbsp; lipid concentration of Wistar rats induced - hyperlipidemia. These rats were divided into four groups, each consisting of five rats. Three of these groups were subsequently induced by (1) jati belanda extract at a dose of 25 mg/kg body weight, (2) jati belanda extract at a dose of 50 mg/kg body weight, and (3) simvastatin. The fourth was the control group. The extract as well as the simvastatin was given orally for 14 days whereas the control group received vehicle. Blood lipid concentration was determined on day 7 and day 14. The result of this study was analyzed statistically using SPSS program. The result showed that the group induced with water extract of jati belanda at a dose of 50 mg/kg body weight had a significantly lower level of total cholesterol and LDL cholesterol than the control group.
Pengaruh Kombinasi Ekstrak Umbi Lapis Bawang Putih dan Ekstrak Rimpang Kunyit terhadap Janin Mencit Swiss-Webster Sukandar, Elin Yulinah; Fidrianny, Irda; Garmana, Afrillia Nuryanti
Jurnal Kedokteran Maranatha Vol 8, No 1 (2008)
Publisher : Universitas Kristen Maranatha

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The influence of solution of garlic (Allium sativum L.) in water and solution of turmeric (Curcuma domestica Val.) in ethanol had been studied in pregnant Swiss-Webster mice. The garlic and turmeric extract were administered orally at dose level combination of 100:100, 500:500, and 1000:1000 mg/kg body weight on day sixth to fifteenth of gestation. The mother was sacrified on day eighteenth day of gestation to observe life fetus, resorption, and growth failure. One third of the fetuses were eviscerated for skeletal staining with alizarin red and two third of the fetuses were hardened with Bouin&rsquo;s fixation solution. No malformation were found in the eviscerated group. Resorption of 1.11 % fetuses and 1.11 % growfail fetuses showed by extract combination of 100:100 mg/kg body weight. Growfail Growth failure of&nbsp; 4.59 % fetuses showed by extract combination of 500:500 mg/kg body weight. Resorption of&nbsp; 0.96&nbsp; % fetuses showed by extract combination of 1000:1000 mg/kg body weight. The conclusion of this study, is that the combination of turmeric and garlic extract of 100:100 mg/kg body weight, which is equivalent to usual dose in human showed safe as herbal medicine, while in higher dose it is not recommended for pregnant woman.
Studi Efek Teratogenik Ekstrak Buah Mengkudu (Morinda citrifolia) pada Tikus Wistar Putih Anggadiredja, Kusnandar; Sukandar, Elin Yulinah; Santosa, Slamet
Jurnal Kedokteran Maranatha Vol 5, No 2 (2006)
Publisher : Universitas Kristen Maranatha

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Pengembangan sediaan fitofarmaka yang berasal dari bahan alam, khususnya tanaman telah banyak dilakukan. Pemakaian luas sediaan fitofarmaka tidak menutup kemungkinan akan penggunaannya oleh wanita hamil. Oleh karenanya perlu dilakukan uji efek teratogenik yang akan memeriksa kemungkinan kelainan morfologik pada fetus. Pada penelitian ini dilakukan uji efek teratogenik ekstrak buah mengkudu (Morinda citrifolia), salah satu tanaman yang banyak digunakan oleh masyarakat dan berasal dari suatu sediaan kapsul ekstrak buah mengkudu pada tikus Wistar. Suspensi ekstrak (dalam pembawa tragakan 2%) pada dosis 27, 500, dan 1000 mg/kg bobot badan diberikan secara oral pada hari ke 8,9 dan 10 kehamilan. Pada hari ke-19 kehamilan, tikus dibedah untuk pengambilan fetus. Pemeriksaan terhadap jaringan lunak maupun kerangka fetus tidak menunjukkan adanya kelainan morfologik akibat pemberian ekstrak buah mengkudu. Hasil ini mengindikasikan bahwa ekstrak buah mengkudu aman untuk digunakan oleh wanita hamil.
Anti-Rheumathoid Arthritis Effect of Water Fraction of Siwalan Fruit (Borassus flabellifer L.) to Mice Induced by Complete Freunds Adjuvants Adnyana, I Ketut; Sukandar, Elin Yulinah; Indriasari, Wenny
Jurnal Medika Planta Vol 2, No 1 (2012)
Publisher : Jurnal Medika Planta

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Background : Rheumathoid arthritis is a systemic autoimmune disorder, characterized by erosive arthritis in synovial joints and chronic symmetrical which causes severe dysfunction and disability. People used Siwalan (Borassus flabellifer L.) for antidiabetogenic, antipyretic, antiinflamation, immunosupressive, antibecterial and fertility for a man. Objective: to determine the anti-rheumatoid arthritis activity of the water fraction fruit siwalan in mice models Rheumathoid Arthritis.Methods: Mice induced intraplantar with Complete Freund&rsquo;s adjuvants on the right foot with a dose of 0.25 ml / animal. Grouping animals were divided into 5 treatment groups, the control group (CFA), the comparison group (methylprednisolone), water fraction group I (dose 22.82 mg / kg), the water fraction II (dose 89.1 mg / kg). Metilprednisolone and water fraction for all dose are given for 20 days. Data were taken on days 1, 5, 10, 15 and 21. The parameters are swelling volume, ankle diameter, scoring macroscopic and back limb histological using Hematoxyllin-eosin staining.Results: Results of data processing using one-way ANOVA on the 15th day of the swelling volume, there were significant differences (p &lt;0.05) between the control group and comparison. There is a trend toward reduction in the swelling volume of water fraction group dose I (0,054 ml &plusmn; 0,0056) and dose II (0,055 ml &plusmn; 0,0028). In the rat foot diameter measurement the 15th day there are significant differences (95% confidence level) between the control group with the comparison group, the water fraction dose I andIII. And there were no significant differences between the comparison groups with all water fraction group. This means that all water fraction group and the comparison group had the effect of decreasing the diameter of the ankle as well. For data analysis macroscopic scoring on the 15th day there are significant differences (Asymp. Sig. &lt;0.05) between the control group with the comparison group, the water fraction dose II. There were no significant differences between the comparison groups with the water fraction dose group I (22.28 mg / kb mm) and II (89.1 mg / kg bw).Conclusion: Water fraction of siwalan has an anti-rheumathoid arthrithis in mouse models of rheumatoid arthritis induced by CFA.
ANTIHYPERLIPIDEMIC AND ANTIDIABETIC EFFECT OF COMBINATION OF GARLIC AND TURMERIC EXTRACT IN RATS Sukandar, Elin Yulinah; Sigit, Joseph I.; Deviana, Riva
Jurnal Medika Planta Vol 1, No 1 (2010)
Publisher : Jurnal Medika Planta

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Introduction: The effect of oral administration of turmeric (Curcuma longa L) rhizomes, and garlic (Allium sativum L) bulbs extracts, and its combination on blood lipoprotein and glucose profile of&nbsp; hyperlipoproteinemic-diabetic male Sprague-Dawley rats had been studied. Methods: The rats were induced hyperlipoproteinemic and diabetic with high fat diet, propylthiouracyl-containing drink, and monohydrate alloxan as a diabetogen. Results: showed that the combination showed higher effect than that of each extract&nbsp; alone. The combination at 100 mg/kg bw&nbsp; of each extract decreased blood glucose,&nbsp; total cholesterol, LDL&nbsp; and triglycerides level by (47,96 &plusmn;&nbsp; 41,25)% (p=0.008),&nbsp; (24,15 &plusmn;&nbsp;&nbsp; 8,89)% (p= 0.001), (8,19 &plusmn;&nbsp;&nbsp; 31,76)% (p=0,065) and&nbsp; (47,85 &plusmn; 20,38)% (p=0.004) respectively while turmeric&nbsp; extract at a dose of 100 mg/kg bw&nbsp; decreased blood glucose, blood total cholesterol, LDL,&nbsp; and triglycerides level by( 41,24 &plusmn;&nbsp; 29,39)% (p= 0.020), (13,01 &plusmn;15,75)% (p=0.056),&nbsp; ( 3,92 &plusmn; 21,09)%&nbsp; (p=0,025)&nbsp; and&nbsp; (17,97 &plusmn; 21,1)% (p=0.201) respectively&nbsp; and&nbsp; garlic extract at a dose of 100 mg/kg bw decreased blood glucose, total cholesterol&nbsp; and triglycerides level by (24,39 &plusmn;&nbsp; 33,19)%&nbsp; (p=0.09),&nbsp; (14,67 &plusmn;13,28)% &nbsp;&nbsp;(p= 0.035),&nbsp; (5,27 &plusmn;&nbsp;&nbsp; 23,38)% (p= 0,334) and&nbsp; (28,18 &plusmn; 7,72)% (p&lt;0.001). respectively. Blood HDL level was 25.83% - increased&nbsp; by the combination at&nbsp; doses of 100 mg/kg bw each&nbsp; (p=0.256). Conclusion: the combination showed higher effect than that of each extract&nbsp; alone.
Influence of Ethanol Extract of Binahong (Anredera cordifolia (Ten.)Steenis) Leaves on Renal Failure Rat Model Sukandar, Elin Yulinah; Qowiyyah, Aun; Minah, Nilna
Jurnal Medika Planta Vol 1, No 2 (2010)
Publisher : Jurnal Medika Planta

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Introduction: Binahong (Anredera cordifolia (Ten.) Steenis) leaves, traditionally, are used to treat various diseases including improvement renal failure.Objective: This study aims to determine the effect of binahong leaves extract to imporve renal function in renal failure conditions.Methods: The reseach includes quality analysis and testing of the effects of binahong leaves extract to renal failure. The ethanol extract of "binahong" leaves were tested on renal failure female Wistar rats induced by gentamycin at a dose of 100mg/kg bw intraperitoneally and captopril at a dose of 10 mg/kg bw orally with blood creatininelevel as parameter.Result: the research showed that the ethanol extract of "binahong" leaves a a dose of 50; 100 and 200 mg/kg bw could repair renal function showed by lowering creatinine level significantly different compared to control group (p&lt;0.05). Bowman capsulle and glomerullus cell were recovered by the extract according to histopathology examination.Conclusion: Ethanol extract of binahong leaves can be used to improve renal failure in rats.
Effect of Methanol Extract Hearhleaf Madeiravine (Anredera cordifolia (Ten.) Steenis) Leaves on Blood Sugar in Diabetes Mellitus Model Mice Sukandar, Elin Yulinah; Qowiyyah, Atun; Larasari, Lady
Jurnal Medika Planta Vol 1, No 4 (2011)
Publisher : Jurnal Medika Planta

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Heartleaf madeiravine (Anredera cordifolia (Ten.) Steenis) leaves, traditionally, are used to treat various diseases including to decrease blood glucose level.Objective: This study aims to determine the effect of heartleaf madeiravine leaves extract in lowering blood glucose level and in repairing a damage of -pancreas cells. Methods : This experimental research uses Swiss Webster mice The extract was tested on diabetic Swiss Webster mice induced by alloxan at a dose of 70 mg/kg bw intravenously . Doses used for treating these mice were 50 mg/kg bw and 200 mg/kg bw orally for 14 days .Blood glucose level of the mice were measured after 7 and 14 days administration of the extract. The pancreas cell of treated mice were observed histologicaly on day 14. Result: The research showed that the methanol extract of heartleaf madeiravine leaves at a dose of 50, 100 and 200 mg/kg bw could lower blood glucose level significantly different compared to control group (p&lt;0.05) either on day 7 or on day 14 and increased the number and repaired the damage of - pancreas cell. Conclusion: Methanol extract of heartleaf madeiravine leaves can be used to treat diabetes mellitus induced by alloxan in mice.
The Effect of Coconut "Genjah Salak" (Cocos Nucifera L) Water and Isotonic Drinks on Blood Glucose Levels Syafriani, Rini; Sukandar, Elin Yulinah; Apriantono, Tommy; Sigit, Joseph I.
Jurnal Medika Planta Vol 1, No 5 (2012)
Publisher : Jurnal Medika Planta

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Introduction: Since years ago the first coconut water has been used as tradicional medicine to cure various diseases. The content of glucose, electrolytes, vitamins, and proteins in the coconut water can serve as a replacement of body fluitds, as an energy source, and rehydration enhancer. Today there are a lot of isotonic drinks products marketed in the community. The drinks usually contain glucose, sodium, and potassium. Isotonic drinks are used in this study is almost the same composition with coconut "genjah salak" water.Objective:&nbsp;to know when the glucose levels increase and decrease after administration of coconut "genjah salak" water and the provision of isotonic drinks. The results of this study is expected to initiate the development of natural isotonic beverage as the replacement of body fluidsMethod: The research method is the human clinical trials testing using double-blind cross-over design of the experiment. The measured parameter is the measurement of glucose tolerance in the body per 15 minutes for 2 hours after the administration of coconut "genjah salak" water and the provision of isotonic drinks without doing physical activities during the measurement. Resullt: The glucose levels in the group given coconut "genjah salak" water and the group given isotonic drinks significantly increased, compared with the control group at the 15th , 30th and 45th minutes, but from 60th to 120th minutes there were no significant differences in the confidence level of &alpha; 0.05. Coconut "genjah salak" water group significantly increase at the 30th, 45th, and 60th minutes, compared with isotonic drinks at the confidence level of &alpha; 0.05. Conclusion: consuming coconut water can maintain and improve glucose levels required to replace body fluids. The body takes about 15-30 minutes to absorb the drinks in order to work effectively, so it is recommended to consume coconut water or isotonic drinks approximately 15-30 minutes before doing physical activities.