SRIANI SUJIPRIHATI
Departemen Agronomi dan Hortikultura, Institut Pertanian Bogor, Jl. Meranti, Kampus IPB Darmaga, Bogor 16680

Published : 68 Documents
Articles

Diallel Analysis using Hayman Method to Study Genetic Parameters of Yield Components in Pepper(Capsicum annuum L.) SYUKUR, MUHAMAD; SUJIPRIHATI, SRIANI; YUNIANTI, RAHMI; UNDANG, .
HAYATI Journal of Biosciences Vol 17, No 4 (2010): December 2010
Publisher : Bogor Agricultural University, Indonesia

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Abstract

One method to obtain genetic information is the diallel cross analysis. The objective of this study was to eavluate the genetic parameters of six inbred pepper (Capsicum annuum L.) using full diallel crosses. The experiment was conducted at IPB Experiment Field, Cikabayan, Darmaga. The design was randomized complete block design (RCBD) using three replications as blocks. Data from generation F1 and parents were analyzed using the Hayman Method. Results indicated that no epistatic effects were significant for all the traits assessed. Additive genetic effects were larger than the dominant effects for yield per plant, fruit length, and  diameter fruit traits. Dominant genetic effects were larger than the additive effects for fruit weight traits. Narrow-sense and broad-sense heritability were high for all the traits assessed. The character of the yield per plant, fruit weight and fruit diameter shows that there were more dominant genes in the parents. There were more recessive genes in parents for the fruit length character. IPB C7 parent was the most recessive genes containing control characters in the yield per plant. In the new improved varieties of high yielding, IPB C7 could be crossed with IPB C9. Employing individual or mass selection breeding should be successful in developing high-productivity lines in this population.
Evaluation of Plant Growth Promoting Rhizobacteria as a Protecting Agent Against Cucumber Mosaic Virus and Chilli Veinal Mottle Virus on Chillipepper TAUFIK, MUHAMMAD; HIDAYAT, SRI HENDRASTUTI; SUASTIKA, GEDE; SUMARAW, SIENTJE MANDANG; SUJIPRIHATI, SRIANI
HAYATI Journal of Biosciences Vol 12, No 4 (2005): December 2005
Publisher : Bogor Agricultural University, Indonesia

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Abstract

This study was conducted to evaluate the effectiveness of plant growth promoting rhizobacteria (PGPR) in protecting chillipepper plant from infection of cucumber mosaic virus (CMV) and chilli veinal mottle virus (ChiVMV). Seven isolates of PGPR, i.e. BC1, BTP2H, BTP3G, BTP3O BTP1, BTP2D, and T1F were applied as seed treatment and soil drench. Plants height, number of branch, and fruits weight were measured every one and ten weeks after virus inoculation. Virus concentration in plants and disease incidence were confirmed by enzyme-linked immunosorbant assay (ELISA). Results showed that inoculation with PGPR improved the seed germination. Eight days after sowing, the percentage of PGPR treated seed germination reached 50-84%; whereas those of untreated seed reached only 18%. In general, PGPR treatment significantly reduced (p < 0.05) the effect of virus infection on plant growth. Two PGPR isolates, i.e. BTP1 and BTP2H, maintained fruit weight of infected plants as good as those of healthy plants. Based on ELISA, PGPR was able to inhibit the disease incidence. The BTP3O and BTP2D isolates even protected the plant from ChiVMV infection. Concentration of salicylic acid and peroxidase were relatively higher on plants treated with PGPR than those without PGPR treatment. This gave an indication that PGPR may act as induction agents for systemic acquired resistance. Therefore, PGPR treatment is a promising strategy to control viral diseases on chillipepper.
Metode Pengusangan Cepat untuk Pengujian Vigor Daya Simpan Benih Cabai (Capsicum annuumL.) Ekowahyuni, Luluk Prihastuti; Sutjahjo, Surjono Hadi; Sujiprihati, Sriani; Suhartanto, Mohamad Rahmad; Syukur, Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 40, No 2 (2012): JURNAL AGONOMI INDONESIA
Publisher : Bogor Agricultural University

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Abstract

The ability of seed to maintain seed quality during storage is called seed vigor. Accurate method for seed vigor testing of pepper seeds is necessary to accurately determine seed storability during seed distribution. The aim of this research was to determine the best accelerated aging method (AAM) of pepper seed. Freshly harvested seeds of Capsicum annuumL., IPB C9 genotype were used in this study. The experiment used randomized complete block design (RCBD) with three replications. The experimental treatments were seed vigor testing methods i.e. natural deterioration test at room temperature in controlled humidity (RH 90-95%), and four AAM (hot water 60 ºC, methanol 20%, ethanol 20%, and high temperature at 40 ºC). The best method was selected using analysis of variance, coeficient of variance, regresion dan t student analysis. AAM using 20% methanol at periods of 0, 2, 4, 6 and 8 hours was the best method of seed vigor testing for pepper seed. Keywords: accelerated aging, deterioration, ethanol, methanol, high temperature
Pendugaan Parameter Genetik pada Beberapa Karakter Kuantitatif pada Persilangan antara Cabai Besar dengan Cabai Keriting (Capsicum annuumL.) Arif, Abdullah Bin; Sujiprihati, Sriani; Syukur, Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 40, No 2 (2012): JURNAL AGONOMI INDONESIA
Publisher : Bogor Agricultural University

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Abstract

Selection method is one of the most important factors in determining the success of pepper breeding programs. Selection method will be effective if it is supported by a complete knowledge of genetic character inheritance. The aim of this research was to investigate the inheritance patterns to quantitative characters using large pepper (P1 (IPB C5)), curly pepper (P2 (IPB C105)), F1, F1R, BCP, BCP2 and F2. The result showed that dichotomous height, days to harvest and weight per fruit characters were not maternally inherited. The additive-dominant genetic model was the most suitable for dichotomous height character. The additive-dominant with influence of interaction additive-dominant and interaction dominant-dominant genetic model was suitable for days to harvest character. The additive-dominant with influence of interaction additive-additive and interaction dominant-dominant genetic model was suitable for the weight of fruit character. Broad-sense and narrow-sense heritabilities range medium for dichotomous height and weight per fruit characters. Heritability of the days to harvest character was high in a broad-sense but was low in the narrow-sense. Keywords: genetic model, heritability, inheritance
Karakter Fisik dan Kimia Buah Pepaya pada Stadia Kematangan Berbeda Suketi, Ketty; Poerwanto, Roedhy; Sujiprihati, Sriani; Sobir, ,; Widodo, Winarso Drajad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 1 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The objective of the experiment was to investigate the physical and chemical characteristics of three stadia of maturity based on a range of peel color from green to yellow or based on percentage of the yellow area of fruit peel (stadium 1 = 25-49 % yellow, stadium 2 = 50-74 % yellow, and stadium 3 = above 75 % yellow) on six genotypes of papaya. Each genotype exhibited different days to maturity for each stadium. The fruits of stadium 1, 2 and 3 for IPB 1 were picked at 130, 135, and 140 days after anthesis (DAA); IPB 10A at 160, 165, and 170 DAA;  IPB 1 x PB 174 at 135, 140 and 145 DAA; while PB 174,  IPB 1 x IPB 10A and IPB 10A x PB 174 were picked at 140, 145 and 150 DAA, respectively. The results indicated that peel firmness was affected by maturity stage on female fruit of IPB 10A. Maturity stage affected chemical characteristics of papaya included total soluble solids (TSS) content (IPB 10A, female fruit of  PB 174, female fruit of IPB 1 x IPB 10A, and female fruit of IPB 1 x PB 174), vitamin C content (hermaphrodite fruit of 10 A, female fruit of IPB 1 x IPB 10A) and juice pH (hermaphrodite fruit of  IPB 1).  IPB 1 genotype can be harvested at all stadia of maturity stage. Hermaphrodite and female fruit of IPB 10 A, female fruit of  PB 174, female fruit of  IPB 1 x IPB 10A and female fruit of IPB 1 x  PB 174 genotype would be better harvested at stadium 3 of maturity stage.   Keywords: Carica papaya, papaya genotype, hermaphrodite fruit, female fruit, fruit quality, fruit maturity stage
Ketahanan terhadap Antraknosa yang Disebabkan oleh Colletotrichum acutatum pada Beberapa Genotipe Cabai (Capsicum annuum L.) dan Korelasinya dengan Kandungan Kapsaicin dan Peroksidase Syukur, Muhamad; Sujiprihati, Sriani; Koswara, Jajah; Widodo, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 37, No 3 (2009): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Anthracnose is one of the most destructive pepper diseases in Indonesia. Colletotrichum acutatum has been identified as a predominant species in pepper fields of Asian countries including Indonesia. The experiment used completely randomized block design with 2 factors and 4 replications. The first factor was 14 genotypes (c-1,2,3,4,5,7,8,9,15,18,19,28,47, and 49, and the second factor was 4 isolates of C. acutatum (PYK 04, BGR 027, MJK 01, and PSG 01). Each experimental unit used 10 green pepper fruits.  Inoculation methods followed the AVRDC procedure and resistance score followed the modified procedure of Yoon method. Symptoms were evaluated five days after inoculation. Disease incidence was evaluated using Yoon method with slight modifications. The experiments showed that C-15 genotype was more resistant to anthracnose than others; C-8 and C-49 genotypes were recorded as susceptible to anthracnose. Except the three genotypes, all other genotypes were recorded as highly susceptible to anthracnose. Capsaicin content and peroxsidase activities were not correlated with resistance to anthracnose.   Key words:  pepper, resistance, anthracnose, Colletotrichum acutatum
Evaluasi Daya Hasil Cabai Hibrida dan Daya Adaptasinya di Empat Lokasi dalam Dua Tahun Syukur, Muhamad; Sujiprihati, Sriani; Yunianti, Rahmi; Kusumah, Darmawan Asta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 1 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The aim of the experiment was to study the performance of yield components of  eight pepper hybrids and their adaptation at four locations in two years (2008 and 2009). The experimental design used was Randomized Complete Block Design (RCBD), three replications as a block was nested in the location. Plant materials were eight hybrids (IPB CH1, IPB CH2, IPB CH3, IPB CH25, and four commercial hybrids were Adipati, Biola, Gada, and Hot Beauty). The IPB CH3 hybrid produced higher fruit width, fruit length, fruit weight, fruit flesh density and yield per plant compared to commercial hybrids. Days to flowering and days to harvesting of IPB CH3 was earlier than that of Hot Beauty. The performance of yield characters in Boyolali, Rembang and Subang was better than that in Bogor. The IPB CH3 hybrid was specifically adapted to Subang and Rembang, while IPB CH1, IPB CH2, IPB CH25 were suitable for Boyolali.   Keywords: fruit, yield components, genotype x location interaction, Capsicum annuum L.
Evaluasi Daya Gabung dan Heterosis Lima Galur Jagung Manis (Zea mays var. saccharata) Hasil Persilangan Dialel Iriany, R. Neni; Sujiprihati, Sriani; Syukur, Muhamad; Koswar, Jajah; Yunus, Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 2 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

  The objective of this research was to estimate general combining ability (GCA), specific combining ability (SCA), and heterosis of five sweet corn inbreds in order to be selected to develop hybrid varieties. Five inbred lines as parent were Mr12/SC/BC4-6-1B-1, Mr14/SC/BC4-6-1B-1, Mr4/SC/BC4-2-1B-1, Mr11/SC/BC4-2-1B-1, and Mr12/SC/BC3-3-1B-1. The experiment was conducted from April to June 2010 using a Randomized Complete Block Design with two replications at Indonesian Cereal Research Institute (ICERI) experimental station, Maros, South Sulawesi. Analyses of GCA and SCA were based on the Griffing’s fixed model of Diallel Design Method I. Heterosis values were predicted based on the average values of their parents whereas heterobeltiosis were predicted based on the average values of the highest parents. Cross B x D (Mr14/SC/BC4-6-1B-1 x  Mr11/SC/BC4-2-1B-1) had  the highest SCA for yield 990.67. Cross A x B (Mr12/SC/BC4-6-1B-1 x Mr14/SC/BC4-6-1B-1) had the highest SCA for ear diameter 0.36. Cross A x D (Mr12/SC/BC4-6-1B-1 x Mr11/SC/BC4-2-1B-1) had the highest heterosis and heterobeltiosis for yield, while Cross A x D (Mr12/SC/BC4-6-1B-1 x Mr11/SC/BC4-2-1B-1) had the highest heterosis and heterobeltiosis for ear  length. Cross A x B (Mr12/SC/BC4-6-1B-1 x Mr14/SC/BC4-6-1B-1) and E x A (Mr12/SC/BC3-3-1B-1 xMr12/SC/BC4-6-1B-1) had the highest heterosis and heterobeltiosis for ear diameter.   Keywords: combining ability, diallel crossing, heterosis, sweet corn 
Pengaruh Perlakuan Ethyl Methane Sulfonate pada Tanaman Cabai (Capsicum annuum L.) dan Ketahanannya Terhadap Chilli Veinal Mottle Virus (ChiVMV) Manzila, Ifa; Hidayat, Sri Hendrastuti; Mariska, Ika; Sujiprihati, Sriani
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 3 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Ethyl Methane Sulfonate (EMS) may induce mutation leading to somaclonal variation if it is used at the appropriate combination of EMS concentration and exposure time. Variation in somaclonal might be valuable as a source of resistance to plant pathogens including  plant viruses. This study was aimed 1) to determine the optimum EMS concentration and incubation time that may induce somaclonal variation in chilli pepper; and 2) to evaluate the resistance of the somaclone to ChiVMV  infection. Shoot-tip explants of five chilli pepper genotypes (Jatilaba, ICPN 12 no. 4, PBC495, Helem, and Gelora) were treated with EMS at combination of different concentrations (0.25%, 0.5%  1.0% and control),  and incubation time (15, 30, 60 min). Subsequently, each explant was grown  in multiplication media (MS media + 5 mg L-1 BAP + 0.5 mg L-1 TDZ), rooting media (MS  media +  1 mg L-1 NAA), and acclimatization media (mixture of soil : sand : compost  2:1:1 w/w). Our results showed that the higher EMS concentration and the longer incubation period the smaller the number of survive explants. The highest survival rate  20.4 %  was achieved with 0.5% EMS in combination with 60 min  incubation period. This treatment combination also showed induction of phenotypic variation. Two somaclonal plants derived from Gelora genotype, designated as somaclones K1 and K2,  survived until fruit development and maturation. A total of 245 progenies of K1 and 243 progenies of K2, respectively were evaluated for their resistance to ChiVMV infection through mechanical inoculation using ChiVMV-Cikabayan isolate. Following the detection of ChiVMV using DAS-ELISA, it was confirmed that  4.09% of the somaclonal progenies were  resistance to ChiVMV.   Keywords:  Capsicum annuum, ChiVMV, ethyl methane sulfonate, induce mutation, resistance
Parametric Stability Analysis for Yield of Chili Pepper (Capsicum annuum L.) Syukur, Muhamad; Sujiprihati, Sriani; Yunianti, Rahmi; Kusumah, Darmawan Asta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 1 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The objective of this study was to identify the stability of seven hybrid chili pepper genotypes that have been developed at Genetics and Plant Breeding Laboratory, Department of Agronomy and Horticulture IPB. The study used eight yield stability analyses and Additive Main Effect Multiplicative Interaction (AMMI) methods. The design was randomized complete block design with three replications as blocks using the genotypes of IPB CH1, IPB CH2, IPB CH3, IPB CH5, IPB CH25, IPB CH28, IPB CH50, and fi  ve commercial varieties, i.e. Adipati, Biola, Gada, Hot Beauty and Imperial. These genotypes were planted at six different locations at Ciherang, Leuwikopo, Tajur, Subang, Rembang and Boyolali. IPB CH28, IPB CH25, IPB CH1 and IPB CH2  were more stable cultivars than IPB CH3, IPB CH5, IPB CH50, Adipati and Biola, which had 10, 9, 8, and 6 out of all 10 stability statistics used, respectively. IPB CH28 and IPB CH25 being the most stable cultivars. IPB CH3 was the best genotype compared to the checks based on pair wise GxE interaction test. Based on post predictive success, the AMMI2 model was able to explain 85.51% of the interaction-influenced variation. The stable genotypes in six locations were IPB CH1, IPB CH2, IPB CH25, IPB CH28, and IPB CH50. IPB CH3 genotype was locally adapted for Subang.   Keywords: chili pepper, multi location trials, yield stability