MUCHTAN SUJATNO
Departemen Farmakologi dan Terapi Fakultas Kedokteran Universitas Padjadjaran

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-Conglycinin Content Obtained from Two Soybean Varieties Using Different Preparation and Extraction Methods

HAYATI Journal of Biosciences Vol 18, No 1 (2011): March 2011
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Soybean is a good source of protein. It has two major fractions, b-conglycinin (7S) and glycinin (11S). b-conglycinin’s function was known to suppress food intake, and this effect may be due to stimulating endogenous cholecystokinin (CCK) release. The aims of this study were to determine the highest content of total b-conglycinin and b-conglycinin sub unit-b level obtained from two varieties of soybean i.e. Wilis and Detam 1 varieties using different preparation and extraction methods. These two soybean varieties were prepared into tempeh. Then the seed and tempeh were extracted using Deak and Panthee methods. There were six extracts analysed using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) and coomassie brilliant blue (CBB) staining. The result was shown that Detam variety and raw seed contained the highest total b-conglycinin level. And Panthee method was the best method for extraction of total b-conglycinin, while Deak method was the best method for extraction of b-conglycinin subunit-b.

The Effect of Red Fruit Oil Toward NF-kB Expression on Colitis-Associated Cancer Mice Model

Journal of the Indonesian Medical Association Vol. 63 No. 3 March 2013
Publisher : Journal of the Indonesian Medical Association

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Abstract

Introduction: Colorectal cancer has provided a paradigm for the association between inflammation and cancer. Activation of nuclear factor-kB (NF-kB) is associated with various type of cancers due to its key role in innate immunity, inflammation, cell proliferation, and cell survival. Red Fruit (Pandanus conoideus Lam.) which contains large amount of antioxidant has been considered as phytopreventive agent. The aim of this research is to examine the effect of buah merah oil towards NF-kB expression in colitis-associated cancer (CAC) mice model.Methods: BALB/c male mice were divided into four groups (n=6). The negative control and red fruit control groups were given aquabidest and red fruit oil, respectively, without CAC induction. The azoxymethane (AOM)/dextran sulfate sodium (DSS) control and red fruit treatment groups were given AOM followed by DSS to induce CAC. The AOM/DSS control group were given aquabidest while the red fruit treatment group were given buah merah oil. NF-kB colon level was measured using Western blot method. Data were analyzed by One-Way ANOVA continued with Tukey HSD (a=0.05).Result: The result showed that the average NF-kB level of red fruit treatment group was significantly decreased compared to the AOM/DSS control group (p<0.000).Conclusion: Red fruit oil decreased NF-kB level in CAC mice model.J Indon Med Assoc.2013;63:106-11Keywords: inflammation, colorectal cancer, NF-kB, red fruit

Transduksi Sinyal Hormon Kolesistokinin sebagai Target untuk Mengatasi Obesitas

Jurnal Kedokteran Maranatha Vol 9, No 2 (2010)
Publisher : Universitas Kristen Maranatha

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Abstract

In the last decade, the number of overweight children and adults has increased all over the world. In Indonesia,  obesity  needs  serious  management  because  it  is  multi-factorial  etiology.  One  of  the predominant  factors  of  obesity  is  excessive-appetite.  This  paper  aims  to  discuss  a  gut  hormone  called cholecystokinin  (CCK),  which  has  been  proven  to  be  able  to  decrease  appetite.  CCK  has  a  short  term anorectic effect, which becomes the reason for the hormone to be one of therapeutic targets of obesity in the future.  CCK  is  produced  in  the  enteroendocrine  cell  along  the  small  gut  mucous  and  the  secretion  is stimulated  by  protein  and  fat  through  signal  transduction  mechanisms  called  G  Protein  Coupling Receptor  (GPCR),  GPR  93.  Then  they  activate  Extracellular  Signal-Related  Kinase  (ERK)  1/2  through Gαq and Gαi signal transduction. Soybean contains a bioactive protein, β-conglycinin (BconP) subunit–β, which  has  been  proven  to  be  the  best  inducer  for  CCK  secretion.  The  well-known  signal  transduction pathway is Gαq-coupled  GPCR,  which  influences  the  calcium  signal  and  activates  Protein  Kinase  C (PKC). However, the downstream signaling to complete the pathway remains unclear and further study will be needed to explore the whole process.   Keywords: signal transduction, cholecystokinin, fat, protein, β-conglycinin

THE COMPARISON OF EFFECT BETWEEN WATER AND ETHANOL EXTRACT OF MENIRAN HERB ON THE AMOUNT OF EOSINOPHIL PERCENTAGE IN ATOPIC DERMATITIS MICE MODEL

Jurnal Medika Planta Vol 1, No 1 (2010)
Publisher : Jurnal Medika Planta

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Abstract

Introduction: Atopic dermatitis is hypersensitivity reaction type I which is followed by the increasing of eosinophil, which has role in pathogenesis chronic allergic inflammation. One of the treatment target of atopic dermatitis is to decrease the amount of eosinophil, so the inflammation reaction decreased. Meniran herb is usually used by community to treat dermatitis. The aim of this research is to find out the effect of water and ethanol extract of meniran herb (Phyllanthus niruri. L) to the amount of eosinophil percentage. Methods: The experimental preclinic research in laboratorium is longitudinal, prospective, and comparatives. The mice that induced allergy with ovalbumin were divided randomly in groups (n=6). Various doses of  water and ethanol extract meniran herb were given to those groups, then compared to the group which is given the ovalbumin only (control positive). The experimental data of the amount of eosinophil percentage was analyzed by ANOVA, which followed by the Duncan test, with α=0,05. Results: the research proved that decreasing of the amount of eosinophil percentage doses 1, 2, 3, 4, water meniran extract consecutively 34.833%, 16.167%, 22.833%, 27.833% whereas doses 1, 2, 3, 4, etanol meniran extract consecutively 31.167%, 21.667%,35.167%, 25.833% compared with the positive control (46.833%) (p<0,05). Water and ethanol extract have the same maximum effectivity at dose 2 (p>0,05). Conclusion: meniran herb water and ethanol extract have same effect in decreasing the amount of eosinophil percentage.

ANTIINFLAMMATORY EFFECT OF DANDELION INTO ATOPIC DERMATITIS MODELS USING MICE

Jurnal Medika Planta Vol 1, No 1 (2010)
Publisher : Jurnal Medika Planta

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Abstract

Introduction: Atopic dermatitis is an inflammation of the skin based on hypersensitivity type I reaction. Medication for atopic dermatiis can be done in conventional or inconventional ways and dandelion (Taraxacum officinale Weber et Wiggers) is one of them. Objective: to determine antiinflammatory effect of dandelion’s aqueous and ethanol extract in reducing the inflammation area and the number of inflammatory cells on mice’s skin tissue with atopic dermatitis induced by ovalbumin. Methods: The characteristic of this research was prospective experimental laboratory, using mice, injected with 0.2 ml ovalbumin 10% intracutaneously, on the first, seventh, and twenty-first days. The mice were given dandelion’s aqueous and ethanol extract with dose 1: 0.0078g/ mice 20g; dose 2: 0.0195g/ mice 20g;  dose 3: 0.039g/ mice 20g; dose 4: 0.078 g/ mice 20g perorally. Results: The results show that dandelion’s aqueous extract with dose 4 (6.640mm2) and ethanol extract with dose 2 (5.013mm2)  were the most effective doses in reducing the inflammation area (p<0,05). The summary result of the number of inflammation cells shows statistically significant on giving the dandelion’s ethanol extract dose 1 and 2 compared with the positive control (p<0,05). The effectivity of ethanol extract equal to the aqueous extract in reducing the inflammation coverage area and the number of inflammation cells. Conclusion: both dandelion’s aqueous and ethanol extract are effective in reducing the inflammation coverage area, ethanol extract effective to reduce the number of inflammation cells. Both dandelion’s aqueous and ethanol extract are equally effective.

Effect of Physalis minina, Linn, Psidium guajava, Linn., Sweitenia mahagoni, Jacq Ethanol Extract Against Blood Glucose Level

Jurnal Medika Planta Vol 1, No 2 (2010)
Publisher : Jurnal Medika Planta

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Abstract

Introduction: Diabetes mellitus (DM) is a group of metabolic disorder that is characterized by hyperglycemia due to insulin secretion or function disorder. Physalis minina, Linn., Psidium guajava, Linn., Sweitenia mahagoni, Jacq are used empirically as hypoglycemic medicine.Objectives: to compare the effect of Physalis minina, Linn., Psidium guajava, Linn., Sweitenia mahagoni, Jacq ethanol extract against the hypogycaemic effect of alloxan induced diabetic mice.Method: Thirty alloxan induced diabetic Swiss Webster strain mice, which fasting blood glucose level was more than 126 mg/gL, were deivided into 5 groups: each was given Physalis minina, Linn. Ethanol extract 1574,9 mg/kgBB mencit, gom arab 1% and glibenklamid 1,3 mg/kgBB for 7 days. The fasting glucose level of thosemice was measured on the eighth day. The data were analyzed using ANOVA and continued with Tukey HSD test.Results: there was highly significant difference between fasting blood glucose decrement of Physalis minina, Linn. ethanol extract group (54,26 +- 4,617%)Psidium guajava, Linn. ethanol extract group (54,44 +- 7,338%), Sweitenia mahagoni, Jacq ethanol extract group (71,3 +- 11,80%) against negative control group (1,4017 +- 4,7456%)(p<0,01).Conclusions: Physalis minina, Linn., Psidium guajava, Linn., Sweitenia mahagoni, Jacq ethanol extract can decrease blood glucose level of mice which were induced by alloxan, Sweitenia mahagoni, Jacq ethanol extract has the best effect.

The Comparison Effect of Aloe vera L., Psidium guajava Linn, Curcuma domestica Val to Colitis Ulcerative Mice Models Histopatology

Jurnal Medika Planta Vol 1, No 2 (2010)
Publisher : Jurnal Medika Planta

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Abstract

Introduction: Ulcerative colitis (UC) is an inflammatory disease limited to the mucosal and submucosal layers of the colon. UC treatments was aimed to decrease inflammation and oxidation process on colon. Nowadays people start using hermal medicine to attentuate inflammation and oxidation process in ulcerative colitis, e.g. Aloe vera, Psidium guajava Linn, Curcuma domestica Val.Objective: to compare the effect of Aloe vera ethanol extract, Psidium guajava Linn ethanol extract, Curcuma domestica Val ethanol extract on histopatology of ulcerative colitis.Methods: experimental comparative research with completely randomized design. Mice were divided on 5 groups (n=6) which were given Aloe vera ethanol extract 700 mg/kgBB and DSS 2,5%; Psidium guajava Linn ethanol extract 1.591,2 mg/kgBB and DSS 2,5%; Curcuma domestica Val ethanol extract 180.816 mg/kgBB and DSS 2,5%; aquadest and DSS 2,5% (positive control); aquadest (negative control). Data were analyze with ANOVA followed by Tukey HSD test with a=0.05.Results: there were a decrement percentage of crypt loss on Aloe vera ethanol extract group (10.5 +- 4,64681%), Psidium guajava Linn ethanol extract group (11 +- 2,09762%), Curcuma domestica Val ethanol extract group (9 +- 3,09839%) compared with positive control group (26.8 +- 1,72240%)(p<0.05). Aloe vera ethanol extract, Psidium guajava Linn ethanol extract, Curcuma domestica Val ethanol extract had same effectivity (p>0.05).Conclusion: Aloe vera ethanol extract, Psidium guajava Linn ethanol extract, Curcuma domestica Val ethanol extract had same effectivity on decreasing percentage of crypt loss on colitis ulcerative histopatology of mice induced by DSS 2.5%.

Penurunan Aktivitas Tirosinase dan Jumlah Melanin oleh Fraksi Etil Asetat Buah Malaka (Phyllantus emblica) pada Mouse Melanoma B16 Cell-Line

Majalah Kedokteran Bandung Vol 45, No 2 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Akumulasi melanin menyebabkan hiperpigmentasi dan bila terdapat pada wajah dapat menimbulkan masalah psikososial. Bahan depigmentasi dari tanaman semakin banyak digunakan. Bahan yang dikembangkan harus efektif menghambat sintesis melanin serta tidak toksik terhadap melanosit. Penelitian ini bertujuan menguji efek fraksi etil asetat buah Phyllanthus emblica (P. emblica) atau buah malaka, terhadap sintesis melanin dengan mengukur jumlah melanin dan aktivitas tirosinase, yang merupakan enzim utama sintesis melanin, secara spektrofotometrik pada kultur sel melanosit mouse melanoma B16 cell-line. Sitotoksisitas diukur dengan metode 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Penelitian dilakukan pada November–Desember 2009 di Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, Korea Selatan. Hasil penelitian menunjukkan aktivitas tirosinase dan jumlah melanin menurun bergantung pada dosis perlakuan berbagai konsentrasi fraksi etil asetat buah P. emblica dengan inhibition concentration (IC) 50 berturutturut 95,63 dan 16,90 μg/mL, serta lethal dose (LD) 50 pada konsentrasi 106,64 μg/mL. Simpulan, fraksi etil asetat buah P. emblica mempunyai potensi sebagai bahan depigmentasi, karena mampu menurunkan sintesis melanin melalui inhibisi aktivitas tirosinase. [MKB. 2013;45(2):118–24]Kata kunci: Aktivitas tirosinase, etil asetat, melanin, Phyllanthus emblicaReduction of Tyrosinase Activity and Melanin Amount by Ethyl Acetate Fraction from Malaka (Phyllanthus emblica) Fruit in Mouse Melanoma B16 Cell-LineMelanin accumulation can lead to hyperpigmentation, and if it occurs on the face can cause psychosocial problem. Depigmenting agents derived from plants are increasingly utilized. Agents being developed have to be effective in inhibiting melanin synthesis and should not be toxic to melanocyte. This study aimed was to examine the effect of ethyl acetate fraction from Phyllanthus emblica (P. emblica) fruit, also known as malaka fruit, towards melanine synthesis, which was measured from the melanin amount and tyrosinase activity, the key regulatory enzyme in melanin synthesis, spectrophotometrically towards melanocytes of mouse melanoma B16 cell-line. The cytotoxic effect towards melanocytes was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. This study was conducted on November−December 2009 in Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, South Korea. The result of this study showed that tyrosinase activity and melanin amount decreased in a dose-dependent manner towards various concentrations of ethyl acetate fraction of P. emblica fruit with inhibition concentration (IC) 50=95.63 and 16.90 μg/mL, respectively and lethal dose (LD) 50 concentration 106.64 μg/mL. In conclusion, ethyl acetate fraction of P. emblica fruit is a potential depigmenting agent, since it can reduce melanin synthesis by inhibition of tyrosinase activity. [MKB. 2013;45(2):119–24]Key words: Ethyl acetate, melanin, Phyllanthus emblica, tyrosinase activity DOI: http://dx.doi.org/10.15395/mkb.v45n2.115

Toksisitas Akut per Oral Ekstrak Etanol Daun Dewa (Gynura pseudochina (Lour.) DC) terhadap Kondisi Lambung Tikus Jantan dan Betina Galur Wistar

Majalah Kedokteran Bandung Vol 44, No 1 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Toksisitas oral akut merupakan efek samping yang terjadi dalam waktu singkat melalui pemberian tunggal peroral ataupun dengan dosis berulang dalam waktu 24 jam dan dapat terjadi pada setiap organ tubuh. Traktus gastrointestinal sangat potensial terhadap paparan agen toksik yang tampak sebagai gambaran erosi dan tukak. Penelitian ini merupakan penelitian eksperimental uji toksisitas akut per oral dan dilakukan di laboratorium Farmakologi Klinik RS Dr. Hasan Sadikin Bandung pada bulan Juli–September 2009. Penelitian ini bertujuan untuk mengetahui toksisitas akut ekstrak etanol daun dewa terhadap lambung dengan menghitung indeks tukak ekstrak etanol daun dewa (Gynura pseudochina (Lour.) DC) pada tikus galur Wistar. Untuk mengatasi dampak negatif, tindakan penelitian ini dilakukan dengan pendekatan 3R (reduction, refinement, dan replacement). Indeks tukak dinilai dengan menentukan jumlah dan diameter tukak melalui pembedahan hewan coba pada hari ke-15 dan dilakukan pemeriksaan secara mikroskopis dengan penilaian histopatologi. Data dianalisis dengan uji Kolmogorov-Smirnov yang dilanjutkan dengan one sample t-test pada Statistical Package for the Social Sciences (SPSS) for windows versi 13.0 pada derajat kepercayaan 95%. Indeks tukak antar kelompok tikus jantan dan betina menunjukkan perbedaan yang bermakna antara kelompok kontrol dan kelompok ekstrak etanol dosis >1,625 g/kgBB (p<0,05). Simpulan, pemberian ekstrak etanol daun dewa pada tikus galur Wistar menyebabkan tukak lambung pada dosis >1,625 g/kgBB. [MKB. 2012;44(1):38–43].Kata kunci: Gynura pseudochina (Lour.) DC, indeks tukak, toksisitas oral akutAcute Oral Toxicity of Ethanol Extract of Dewa Leaf (Gynura pseudochina (Lour.) DC) to Male and Female Wistar Rats’ Gaster MucosaAcute oral toxicity is the adverse effects occurring within a short time of oral administration of a single dose of a substance or multiple doses given within 24 hours through body’s organs. Gastrointestinal tract potentially influenced by toxic materials exposure, shown as gastric erosion and ulcer. This is an acute oral toxicity experimental research, held in Pharmacology laboratorium of Dr. Hasan Sadikin Hospital on July–September 2009. This research aims to determine ethanol extract of Dewa leaf acute toxicity to gaster by measuring ulcer index of ethanol extract of Dewa leaf (Gynura pseudochina (Lour.) DC) in Wistar rats. This research approached with 3R (reduction, refinement and replacement) to overcome negative impact. Ulcer index determined by ulcer’s quantity and diameter due to rats necropsy at 15th day, and histopathology examined. The results are statistically analyzed using Kolmogorov-Smirnov test and then continued with one sample T-test on Statistical Package for the Social Sciences (SPSS) for windows version 13.0, at 95% confidence interval level. Ulcer index significantly different between control group and ethanol extract of Dewa leaf group dose >1.625 g/kgBB (p<0.05). In conclusion, ethanol extract of Dewa leaf on Wistar rats cause gastric ulcer at dose >1.625 g/kgBB. [MKB. 2012;44(1):38–43]Key words: Acute oral toxicity, Gynura pseudochina (Lour.) DC, ulcer index DOI: http://dx.doi.org/10.15395/mkb.v44n1.71 

PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN SENDOK (PLANTAGO MAYOR L.) TERHADAP PENGHAMBATAN OVEREKSPRESI GEN REGENERATING-1, PENINGKATAN KADAR CASPASE-3 DAN GAMBARAN HISTOPATOLOGI JARINGAN MUKOSA LAMBUNG TIKUS MODEL HIPERGASTRINEMIA - The Effect of Ethanol Extracts of Plantain (Plantago Mayor L.) on Inhibition of Over-expression of Regenerating-1 Gene, Increasing Caspase-3 Level and Histopatologic Feature of Gastric Mucosa of Hypergastrinemic Rat Model

Indonesian Journal of Applied Sciences Vol 4, No 1 (2014)
Publisher : Universitas Padjadjaran

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Abstract

AbstrakEkstrak etanol daun sendok mengandung banyak zat aktif, termasuk triterpenoid dan flavonoid yang memiliki efek anti proliferatif, anti angiogenesis dan menginduksi apoptosis sel kanker. Hipergastrinemia dapat menginduksi over-ekspresi Gen Regenerating 1, menekan produksi Caspase 3 dan perubahan histopatologi jaringan mukosa lambung. Penelitian ini menganalisis pengaruh pemberian ekstrak etanol daun sendok terhadap penghambatan over ekspresi gen Regenerating 1, peningkatan kadar Caspase 3 dan perubahan histopatologi  jaringan mukosa lambung tikus model hipergastrinemia. Penelitian ini dilakukan secara eksperimental dengan post test only with control group design. 28 tikus putih dibagi menjadi empat kelompok. Kelompok I (kontrol) diberi Omeprazol dosis 8mg/200gBB tikus/hari, Ciprofibrate dosis 12,5mg/200gBB tikus/hari dan 3 kelompok studi (Kelompok II, III dan IV diberi Omepr azol dosis 8mg/200gBB tikus/hari, Ciprofibrate dosis 12,5mg/200gBB tikus/hari dan ektrak etanol daun sendok masing-masing dosis 50mg, 100 mg dan 200mg per 200gBB tikus/hari). Semua hewan coba dikorbankan dibawah pengaruh anestesi setelah perlakuan selama 2 bulan. Sampel darah dan gaster hewan coba diambil untuk pemeriksaan kadar gastrin dan Caspase 3 dengan metode ELISA, ekspresi Gen Regenerating1 dengan RT-PCR serta histopatologi jaringan mukosa lambung. Kadar gastrin hewan coba meningkat 3-4 kali kadar gastrin normal (>169 pg/mL). Rerata ekspresi Gen Regenerating 1 terendah terjadi pada Kelompok III (1,226±0,108) Analisis statistik menunjukkan terdapat perbedaan ekspresi Gen Regenerating 1 secara bermakna antara Kelompok III dengan kontrol (nilai p= 0,016;IK 95%). Rerata kadar Caspase 3 tertinggi terdapat pada IV (0,603±0,172ng/mL). Analisis statistik menunjukkan terdapat perbedaan kadar Caspase 3 secara bermakna antara kelompok IV dan Kelompok lainnya (nilai p <0,05;IK 95%). Gambaran histopatologi mukosa lambung menunjukan atropi dan tidak terdapat perbedaan ketebalan jaringan mukosa lambung secara bermakna diantara kelompok penelitian (nilai p=0,092; IK95%). Pemberian ekstrak etanol daun sendok mencegah over-ekspresi Gen Regenerating 1 dengan dosis 100mg/200gBB/hari, meningkatkan kadar Caspase 3 dengan dosis 200mg/200gBB/hari tetapi pada dosis penelitian yang digunakan tidak dapat menghambat perubahan histopatologi jaringan mukosa lambung tikus model hipergastrinemiaKata Kunci: Ekstrak etanol daun sendok, Over ekspresi Gen Regenerating 1, Caspase 3, Histopatologi mukosa lambung, Model HipergastrinemiaAbstractPlantago major L has a lot of active substances, including triterpenoid  and flavonoid  that show anti proliferative effect, anti angiogenesis and induce cancer cells apoptosis. Hypergastrinemia able to induce overexpression of Regenerating 1 gene, inhibit activation of caspases include Caspase 3 and cause histopathologic alteration  of gastric mucosal tissues. This study was to evaluate  the effect of Plantago major L extract.on inhibition of Regenerating 1 gene overexpression, increasing of Caspase-3 level and histopathologic alteration of gastric mucosal tissues on hypergastrinemic rat (Rattus norvegicus) model. By experimental study and post test only with control group design,  28 of rats was divided in to 4 groups. Group I  as a negative control was given Omeprazole 8mg/200gbw rat and Ciprofibrate dose 12,5 mg/200gbw rat/day and aquadest per oral. Group II was given Omeprazole 8mg/200gbw rat and Ciprofibrate dose 12,5 mg/200gbw rat/day and Plantago major L. extract 50mg/200gbw rat/day per oral. Group III was given Omeprazole 8mg/200gbw rat and Ciprofibrate dose 12,5 mg/200gbw rat/day and Plantago major L. extract 100mg/200gbw rat/day per oral. Group IV was given Omeprazole 8mg/200gbw rat and Ciprofibrate  dose 12,5 mg/200gbw  rat/day  and Plan tago major  L. extract  200mg/200gbw rat/day per oral. They were killed after 2 month intervention under anaesthetic. Blood sample and gaster organs were collected for measurement of gastrin and caspase 3 level by using ELISA method, expression of Regenerating 1 gene by RT-PCR and histopatological examination of gastric mucosal tissues. There were increasing of gastrin level of rats up to 3-4 times fold compared to normal level and  to indicate hypergastrinemic condition. The lowest average of Regenerating 1 gene expression was found on Group III (1,226±0,108). Statistic analysis show that there were significantly difference of Regenerating 1 gene expression between group III and Control (p= 0,016; 95%CI) The highest average of Caspase-3 level was found on Group IV(0,603±0,172ng/mL) and there were significantly difference of caspase-3 level between group IV and the other Groups (p< 0,05; 95%CI). There were atrophic process on gastric mucosal tissue but there were not significantly difference of gastric mucosal thickness between group  (p= 0,092; 95%CI). This study can be concluded that administration of Plantago major L extract able to inhibit overexpression of  Regenerating 1 gene by dose 100mg/200gbw rat/day, to increase Caspase 3 level by dose 200mg/200gbw rat/day but can not to inhibit histopathologic alteration of gastric mucosal tissues by using all of the doses.  Keyword: Ethanol extract of Plantago major L, Regenerating 1 gene overexpression, Caspase 3, gastric mucosal histopatology, Hypergastrinemia model