. SUHARSONO
Program Studi Teknik Geofisika, Fakultas Teknologi Mineral, UPN “Veteran” Yogyakarta Jl. SWK 104 Lingkar Utara Condongcatur, Depok, Sleman Yogyakarta 55283

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Physiological and Biochemical Responses to Aluminum Stress in the Root of a Biodiesel Plant Jatropha curcas L. TISTAMA, RADITE; WIDYASTUTI, UTUT; SOPANDIE, DIDY; YOKOTA, AKIHO; AKASHI, KINYA; SUHARSONO, .
HAYATI Journal of Biosciences Vol 19, No 1 (2012): March 2012
Publisher : Bogor Agricultural University, Indonesia

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Abstract

We investigated J. curcas responses to aluminum stress, histochemically and biochemically. Histochemical stainings were observed to analysis aluminum accumulation, lipid peroxidation and the loss of plasma membrane integrity on the surface and tissue of the root apex. Enzymatic analysis was conducted to measure malate content in leaf, root and malate efflux in the medium. We used M. malabathricum as a comparison for Al-tolerance plant. J. curcas root elongation was inhibited by 0.4 mM AlCl3, while M. malabathricum root elongation was inhibited by 0.8 mM AlCl3 treatment. Inhibition of root elongation has high correlation with Al accumulation in the root apex, which caused lipid degradation and cell death. Generally, malate content in J. curcas leaf and root was higher than that in M. malabathricum. In the contrary malate efflux from the root into the medium was lower. J. curcas root has a different pattern compared to M. malabathricum in malate synthesis and malate secretion when treated with a different Al concentration. We categorized J. curcas acc IP3 as more sensitive to aluminum than M. malabathricum.
In Vitro Growth and Rooting of Mangosteen (Garcinia mangostana L.) on Medium with Different Concentrations of Plant Growth Regulator HARAHAP, FAUZIYAH; POERWANTO, ROEDHY; SUHARSONO, .; SURIANI, CICIK; RAHAYU, SUCI
HAYATI Journal of Biosciences Vol 21, No 4 (2014): Desember 2014
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Propagation of mangosteen is challenging for many reasons, including limited seed set, slow rate of seedling growth, and difficulty with root formations. The objective of this research was to find the best combination of medium and plant growth regulator for in vitro growth and rooting of mangosteeen seed. Various types of explant (a whole seed; seed divided into 2, 3, and 4 cross sections; seed divided into 2, 3, and 4 longitudinal sections) were treated with five concentrations of benzyl amino purine (BAP; 0, 2.5, 5, 7.5, 10 mg/L) for shoot induction in ½ Nitrogen (N) Murashige and Skoog (MS) medium. The shoots were rooted on MS and woody plant medium (WPM) media with several combinations of indole butyric acid (IBA) and naphtalene acetic acid (NAA). Treatments for root induction were applied as follows: (i) low dose, given during induction of rooting, (ii) soaking the base of the shoots in medium treated with a high dose of auxin for 5 days, and then growing the shoots in MS ½ N with 1 mg/L NAA +  1 mg/L BAP medium. Our result show that BAP positively affected mangosteen bud growth. The best medium for mangosteen shoot regeneration was found to be  MS ½ N  + 5 mg/L BAP. This medium induced  the highest number of shoots from the seed explant cut into four cross sections. We found the best medium to induce in vitro rooting of mangosteen shoot was MS ½ N + 3 mg/L indole butiric acid (IBA) + 4 mg/L NAA medium. Some treatment negatively affected growth. Soaking the mangosteen shoot base in a medium with an overly high dose of auxin seemed to disrupt and inhibit growth of the mangosteen shoot.
Uji Adaptasi Galur Harapan Kedelai di Lahan Podsolik Merah Kuning Kabupaten Bangka Handara, N; Suharsono, .; Mustikarini, ED
ENVIAGRO Vol 7, No 2 (2014): ENVIAGRO
Publisher : Universitas Bangka Belitung

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Abstract

The purpose of this study was to determine the productivity and adaptability of ten soybean genotypes in red-yellow podzolic soils. The experiment conducted in Balunijuk village, Merawang subdistrict, Bangka on July-October 2010. Experimental design used randomized complete block design with 10 genotypes soybean replicated three times. Tested genotypes consisted of six promising lines (KH 8, KH 9, KH 31, KH 38, KH 55, and KH 71) and 4 national varieties (Slamet, Anjasmoro, Wilis and Tanggamus) as comparison. The results indicated that there were significant differences among genotypes for character plant productivity, seed size, plant height, number of root nodules, effective number of root nodules, number of pods, number of productive branches, and sheer number of seeds. Entire promising lines tested had a larger grain size than the check varieties, and the largest grain size and the highest productivity was obtained in KH 71. Therefore, KH 71 considered more promising to be released as new varieties for red-yellow podzolic soils.
The Distribution and Abundance of Black Band Disease and White Syndrome in Kepulauan Seribu, Indonesia Johan, Ofri; Bengen, Dietriech Geoffrey; Zamani, Neviaty Putri; Suharsono, .; Sweet, Michael John
HAYATI Journal of Biosciences Vol 22, No 3 (2015): July 2015
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Coral diseases that have emerged since the early 1970s have caused significant regional ecological impacts. However, there has been a paucity of research into coral disease in South-East Asia, including Indonesia. This study provides baseline coral disease data in the Kepulauan Seribu Marine National Park. In this study we show a positive correlation between overall coral cover and the dominant reef building coral Montipora spp. and found two main diseases, black band disease (BBD) and WS, were highly prevalent throughout all reefs. Based on spatial location, the highest abundance of BBD (0.08 col./m2) was found at sites nearer (zone 1) to the mainland, whilst for WS (0.05 col./m2) highest abundance was found at middle sites (zone 2). According to the temporal data, the highest abundance of BBD (0.77 col./m2) was found during the transition period (between wet and dry seasons), whereas for WS higher abundance occurred within the dry season (0.07 col./m2). There was a significant difference in disease abundance among seasons which was correlated with increasing temperature and light intensity along with variations in total organic matters, nitrite and phosphate levels. Moreover, the middle sites experienced additional stress from the waste material originating from the mainland.
Establishment of Hevea brasiliensis lines overexpressing genes involved in ethylene signalling pathway LESTARI, Retno; RIO, Maryannick; MARTIN, Florence; LECLERCQ, Julie; DESSAILLY, Florence; SUHARSONO, .; MONTORO, Pascal
E-Journal Menara Perkebunan Vol 84, No 1: Oktober 2016
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

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Abstract

The gaseous plant hormone ethylene has a wide variety of applications in agriculture and horticulture. Ethylene Response Factors (ERF) are the last transcription factors of the ethylene signalling pathway and control a large number of ethylene-responsive genes. Two Hevea brasiliensis ERF, HbERF-IXc4 and HbERF-IXc5, are orthologs to ERF1 a key regulator at the crosstalk of ethylene and jasmonate signalling pathways. These genes were suggested to play an important role in regulating latex cell metabolism in response to tapping and ethephon stimulation. In this study, transgenic lines overexpressing HbERF-IXc4 and HbERF-IXc5 under control of 35S CaMV and HEV2.1 promoter have been conducted. Transgenic Hevea lines were obtained by Agrobacterium tumefaciens-mediated genetic transformation. The somatic embryogenesis process was affected by these modifications. Agrobacterium tumefaciens genetic transformation procedure has been developed from friable callus line for clone PB260. Hevea callus was sub-cultured as small aggregates on paromomycin selection medium. Transgenic callus lines were established from sub-aggregates showing full GFP activity. Ten transgenic lines were confirmed as transgenic by Southern blot hybridization. This result showed successfully establishment of H. brasiliensis transgenic lines. Further plant regeneration and characterization were necessary to understand the function HbERF-IXc4 and HbERF-IXc5 in latex.
IMPROVEMENT METHOD OF GENE TRANSFER IN Kappaphycus alvarezii Triana, St. Hidayah; Alimuddin, .; Widyastuti, Utut; Suharsono, .; Suryati, Emma; Parenrengi, Andi
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 8, No 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Bogor Agricultural University

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Abstract

Method of foreign gene transfer in red seaweed Kappaphycus alvarezii has been reported, however, li-mited number of transgenic F0 (broodstock) was obtained. This study was conducted to improve the method of gene transfer mediated by Agrobacterium tumefaciens in order to obtain high percentage of K. alvarezii transgenic. Superoxide dismutase gene from Melastoma malabatrichum (MmCu/Zn-SOD) was used as model towards increasing adaptability of K. alvarezii to environmental stress. The treat-ments were the culture media and recovery duration, and each treatment consisted of three replica-tions. The best method was co-cultivation using liquid media, then recovery was conducted in liquid media for 10 days. That treatment allowed higher transformation percentage (90%), regeneration effi-ciency (90%), putative bud efficiency (100%), number of buds and explants sprouted (100%) and transgenic explants (100%). The transgenic explants showed an amplification PCR product of Mm-Cu/Zn-SOD gene fragment, whereas the non-transgenic explants showed no amplification product.  All results revealed that suitable method of transgenesis for K. alvarezii has been developed. Keywords:       Agrobacterium tumefaciens, culture media, Kappaphycus alvarezii, recovery duration, transformation
Establishment of Hevea brasiliensis lines overexpressing genes involved in ethylene signalling pathway LESTARI, Retno; RIO, Maryannick; MARTIN, Florence; LECLERCQ, Julie; DESSAILLY, Florence; SUHARSONO, .; MONTORO, Pascal
E-Journal Menara Perkebunan Vol 84, No 1: Oktober 2016
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Original Source | Check in Google Scholar | Full PDF (480.251 KB)

Abstract

The gaseous plant hormone ethylene has a wide variety of applications in agriculture and horticulture. Ethylene Response Factors (ERF) are the last transcription factors of the ethylene signalling pathway and control a large number of ethylene-responsive genes. Two Hevea brasiliensis ERF, HbERF-IXc4 and HbERF-IXc5, are orthologs to ERF1 a key regulator at the crosstalk of ethylene and jasmonate signalling pathways. These genes were suggested to play an important role in regulating latex cell metabolism in response to tapping and ethephon stimulation. In this study, transgenic lines overexpressing HbERF-IXc4 and HbERF-IXc5 under control of 35S CaMV and HEV2.1 promoter have been conducted. Transgenic Hevea lines were obtained by Agrobacterium tumefaciens-mediated genetic transformation. The somatic embryogenesis process was affected by these modifications. Agrobacterium tumefaciens genetic transformation procedure has been developed from friable callus line for clone PB260. Hevea callus was sub-cultured as small aggregates on paromomycin selection medium. Transgenic callus lines were established from sub-aggregates showing full GFP activity. Ten transgenic lines were confirmed as transgenic by Southern blot hybridization. This result showed successfully establishment of H. brasiliensis transgenic lines. Further plant regeneration and characterization were necessary to understand the function HbERF-IXc4 and HbERF-IXc5 in latex.
In Vitro Growth and Rooting of Mangosteen (Garcinia mangostana L.) on Medium with Different Concentrations of Plant Growth Regulator HARAHAP, FAUZIYAH; POERWANTO, ROEDHY; SUHARSONO, .; SURIANI, CICIK; RAHAYU, SUCI
HAYATI Journal of Biosciences Vol 21, No 4 (2014): December 2014
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (943.386 KB)

Abstract

Propagation of mangosteen is challenging for many reasons, including limited seed set, slow rate of seedling growth, and difficulty with root formations. The objective of this research was to find the best combination of medium and plant growth regulator for in vitro growth and rooting of mangosteeen seed. Various types of explant (a whole seed; seed divided into 2, 3, and 4 cross sections; seed divided into 2, 3, and 4 longitudinal sections) were treated with five concentrations of benzyl amino purine (BAP; 0, 2.5, 5, 7.5, 10 mg/L) for shoot induction in ? Nitrogen (N) Murashige and Skoog (MS) medium. The shoots were rooted on MS and woody plant medium (WPM) media with several combinations of indole butyric acid (IBA) and naphtalene acetic acid (NAA). Treatments for root induction were applied as follows: (i) low dose, given during induction of rooting, (ii) soaking the base of the shoots in medium treated with a high dose of auxin for 5 days, and then growing the shoots in MS ? N with 1 mg/L NAA +? 1 mg/L BAP medium. Our result show that BAP positively affected mangosteen bud growth. The best medium for mangosteen shoot regeneration was found to be? MS ? N? + 5 mg/L BAP. This medium induced? the highest number of shoots from the seed explant cut into four cross sections. We found the best medium to induce in vitro rooting of mangosteen shoot was MS ? N + 3 mg/L indole butiric acid (IBA) + 4 mg/L NAA medium. Some treatment negatively affected growth. Soaking the mangosteen shoot base in a medium with an overly high dose of auxin seemed to disrupt and inhibit growth of the mangosteen shoot.
Physiological and Biochemical Responses to Aluminum Stress in the Root of a Biodiesel Plant Jatropha curcas L. TISTAMA, RADITE; WIDYASTUTI, UTUT; SOPANDIE, DIDY; YOKOTA, AKIHO; AKASHI, KINYA; SUHARSONO, .
HAYATI Journal of Biosciences Vol 19, No 1 (2012): March 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Original Source | Check in Google Scholar

Abstract

We investigated J. curcas responses to aluminum stress, histochemically and biochemically. Histochemical stainings were observed to analysis aluminum accumulation, lipid peroxidation and the loss of plasma membrane integrity on the surface and tissue of the root apex. Enzymatic analysis was conducted to measure malate content in leaf, root and malate efflux in the medium. We used M. malabathricum as a comparison for Al-tolerance plant. J. curcas root elongation was inhibited by 0.4 mM AlCl3, while M. malabathricum root elongation was inhibited by 0.8 mM AlCl3 treatment. Inhibition of root elongation has high correlation with Al accumulation in the root apex, which caused lipid degradation and cell death. Generally, malate content in J. curcas leaf and root was higher than that in M. malabathricum. In the contrary malate efflux from the root into the medium was lower. J. curcas root has a different pattern compared to M. malabathricum in malate synthesis and malate secretion when treated with a different Al concentration. We categorized J. curcas acc IP3 as more sensitive to aluminum than M. malabathricum.