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Journal : Indonesian Journal of Chemistry

DISSOLUTION BEHAVIOR, STABILITY AND ANTI-INFLAMMATORY ACTIVITY OF KETOPROFEN COATED TRIPOLYPHOSPHATE MODIFIED CHITOSAN NANOPARTICLE Hardi, Jaya; Sugita, Purwantiningsih; Ambarsari, Laksmi
Indonesian Journal of Chemistry Vol 13, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.003 KB) | DOI: 10.22146/ijc.21298

Abstract

Ketoprofen coated tripolyphosphate modified chitosan nanoparticle was resulted from ionic gelation process by ultrasonication, centrifugation, and spray dry methods. Particle was analyzed by particle Size Analyzer. Dissolution and stability of them have conducted by Row Hansen methods at 37±0.5 °C and 150 rpm and using climatic chamber at 40±2 °C and relative humidity 75±5% for 3 months, respectively. Anti-inflammatory activities have conducted by Plate Reader methods. The result showed that B formulae have particles diameter of 563.1±157.5 nm and entrapment efficiency of 89.663%, respectively. Dissolution behavior showed that ketoprofen restrained released within acid medium and maximum released within base medium of 11.35-15.87% and 45.73-99.25%, respectively. Kinetically, dissolution of ketoprofen within intestinal and gastric pH condition was Korsmeyers-Peppas kinetic models. While, stability behavior showed that B formula has ketoprofen percentage left in nanocapsule after 3 months greater than 90% with water content and shelf life of 19.68%, and 14.19 week, respectively, and degradation kinetic model followed Avrami-Eroveef equation. Anti-inflammation test showed that B formula had the COX-2 inhibitory activity of 90.86% is higher than free ketoprofen.
STABILITY OF KETOPROFEN COATED BY CHITOSAN-GUAR GUM GEL Sugita, Purwantiningsih; Srijanto, Bambang; Arifin, Budi; Setyowati, Ellin Vina
Indonesian Journal of Chemistry Vol 9, No 3 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (356.602 KB) | DOI: 10.22146/ijc.21504

Abstract

The coating stability of ketoprofen by chitosan-guar gum gel has been studied. Into 228.6 mL of 1.75% (w/v) chitosan solution in 1% (v/v) acetic acid, 38.1 mL of guar gum (gg) solution was added with concentration variation of 0.35, 0.55, and 0.75% (w/v) for ketoprofen microcapsules, and stirred with magnetic stirrer until homogenous. Afterwards, 7.62 mL of glutaraldehyde (glu) was added slowly under stirring, with concentrations varied: 3, 3.5, and 4% (v/v). All mixtures were shaked for 20 min for homogenization. Into each microcapsule mixture for ketoprofen, a solution of 2 g of ketoprofen in 250 mL of 96% ethanol was added. Every mixture was then added with 5 mL of 2% Tween-80 and stirred with magnetic stirrer for an hour at room temperature. Conversion of suspension into fine powders/granules (microcapsules) was done by using spray dryer. Every microcapsule formula was packed into capsules, as much as 100 g per capsule. The capsules were contained in 100-mL dark bottles and the bottles were kept in climatic chamber at (40 ± 2) °C and RH (75 ± 5) % for 3 months. The microcapsule stabilities were tested chemically and physically. The result showed that formulation of ketoprofen preparation composed of 1.75% (w/v) chitosan, 0.35% (w/v) gg, and 3.50% (v/v) glu, was relatively the best, with ketoprofen percentage left in microcapsule after 3 months, degradation rate constant, and shelf life of 80.33%, 0.0351 % week-1, and 18.92 months, respectively. Reaction kinetic model for this formula followed Prout-Tompkins equation and the degradation of ketoprofen was seem to follow autocatalytic reaction mechanism controlled by the formation and growth of reaction core.   Keywords: Ketoprofen, chitosan-guar gum gel
DIFFUSION BEHAVIOR OF KETOPROFEN THROUGH CHITOSAN-ALGINATE MEMBRANES Sugita, Purwantiningsih; Asnel, Rini Siswati; Arifin, Budi; Wukirsari, Tuti
Indonesian Journal of Chemistry Vol 10, No 3 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.425 KB) | DOI: 10.22146/ijc.21429

Abstract

Chitosan-alginate membrane diffusion behavior has been investigated for its application in drugs delivery system. Ketoprofen diffusion behavior assay were performed at 37 and 42 °C to membrane thickness (h) and donor cell concentration of ketoprofen variations (A). The results showed that equilibrium concentrations (Cs) of ketoprofen equation was 27.0087 + 0.09067T - 1.7499h + 0.1030A + 0.0161h2 - 0.0022A2 + 0.0040Th - 0.0018TA + 0.0095hA. The value of Cs was closer to the expected therapy concentration at 50 and 75 mg/L with thin membrane (10-34 μm). Based on Higuchi equation, the model for J and D were J = 11.0849 - 0.2713T - 0.3132h - 0.7461Cs - 0.0096A - 0.0001h2 - 0.0131Cs2 + 0.0002A2 + 0.0084Th + 0.0275TCs - 0.0018TA - 0.0059hCs + 0.0021hA + 0.0037CsA with R2 = 97.9% and D = -12.5000 + 0.2266T + 0.1313h + 0.1538Cs + 0.1200A - 0.0009h2 + 0.0240 Cs2 - 0.0009A2 + 0.0015Th - 0.0150 TCs - 0.0011TA - 0.0096hCs + 0.0004hA + 0.0039CsA with R2 = 98.7%, respectively. The two dimensional contour maps of J versus A and h, both at 37 and 42 °C, showed an increasing of J value as A, h, or T increased.
Significance of Glucose Addition on Chitosan-Glycerophosphate Hydrogel Properties Susanthy, Dian; Sugita, Purwantiningsih; Achmadi, Suminar Setiati
Indonesian Journal of Chemistry Vol 16, No 1 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.767 KB) | DOI: 10.22146/ijc.21179

Abstract

Chitosan-glycerophosphate hydrogel can be used as dental scaffold due to its thermosensitivity, gelation performance at body temperature, suitable acidity for body condition, biocompatibility, and ability to provide good environment for cell proliferation and differentiation. Previous study showed that glucose addition to the chitosan solution before steam sterilization improved its hydrogel mechanical strength. However, the effectiveness of glucose addition was still doubted because glucose might undergo Maillard reaction in that particular condition. The aims of this study are to confirm whether the glucose addition can increase the hydrogel mechanical strength and gelation rate effectively and also to compare their performance to be dental scaffold. This research was performed through several steps, namely preparation of chitosan-glycerophosphate solution, addition of glucose, gelation time test, gel mechanical strength measurement, functional group analysis, and physical properties measurements (pH, viscosity, and pore size). The result showed that glucose addition did not improve the hydrogel mechanical strength and gelation rate, neither when it was added before nor after steam sterilization. Glucose addition before steam sterilization seemed to trigger Maillard reaction or browning effect, while glucose addition after steam sterilization increased the amount of free water molecules in the hydrogel. Chitosan and glycerophosphate interact physically, but interaction between chitosan and glucose seems to occur chemically and followed by the formation of free water molecules. Glucose addition decreases the solution viscosity and hydrogel pore size so the hydrogel performance as dental scaffold is lowered.