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0.486
P-Index
Sunarjati Sudigdoadi
Department of Microbiology and Parasitology, Faculty of Medicine Universitas Padjadjaran
Articles
5
Documents
Methicillin-Resistant Staphylococcus aureus pada Penderita Dermatitis Atopik dan Sensitivitasnya terhadap Mupirosin Dibandingkan dengan Gentamisin

Majalah Kedokteran Bandung Vol 45, No 1 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) mulai dijumpai pada penderita dermatitis atopik (DA) derajat sedang hingga berat dan bersifat multiresisten terhadap antibiotik topikal. Gentamisin telah digunakan secara luas, sedangkan mupirosin merupakan pilihan utama eradikasi MRSA. Penelitian ini bertujuan untuk mengetahui kolonisasi MRSA pada penderita DA dan sensitivitasnya terhadap mupirosin dibandingkan dengan gentamisin di Poliklinik Departemen Ilmu Kesehatan Kulit dan Kelamin Rumah Sakit Umum Pusat Dr. Hasan Sadikin Bandung. Survei potong lintang analitik dilakukan selama 19 September–31 Oktober 2011 terhadap 22 penderita DA derajat sedang hingga berat sesuai dengan Scoring Atopic Dermatitis objektif dan 22 individu sehat. Seluruh subjek dilakukan pemeriksaan apusan kulit untuk deteksi MRSA dan uji sensitivitas terhadap mupirosin dan gentamisin. Pada kedua kelompok masing-masing didapatkan 10 laki-laki dan 12 perempuan. Pada kelompok penderita DA didapatkan DA derajat sedang (10/22) dan berat (12/22), dengan lesi akut (14/22) dan kronik (8/22). Pertumbuhan S. aureus didapatkan pada 17/22 penderita DA dengan sensitivitas terhadap mupirosin 14/17 dan gentamisin 11/17 penderita. Pada individu sehat tidak didapatkan pertumbuhan koloni S. aureus. Galur MRSA ditemukan pada 3/17 penderita DA dan dianggap berbeda secara bermakna bila dibandingkan dengan individu sehat (p<0,01), galur ini bersifat sensitif terhadap mupirosin pada 2/3 penderita dan seluruhnya resisten terhadap gentamisin. Simpulan, kolonisasi MRSA perlu dicurigai pada penderita DA derajat sedang hingga berat, sehingga perlu pengobatan tambahan antibiotik topikal mupirosin. [MKB. 2013;45(1):36–44]Kata kunci: Dermatitis atopik, gentamisin, MRSA, mupirosinMethicillin-Resistant Staphylococcus aureus in Atopic Dermatitis Patients and Its Sensitivity to Mupirocin Compared to GentamycinMethicillin-resistant Staphylococcus aureus (MRSA) is found in moderate to severe atopic dermatitis (AD) and is multiresistant against topical antibiotic. Gentamycin is widely used while mupirocin is the first line therapy to eliminate MRSA. This research is intended to observe the colonization of MSRA case in AD patients and its sensitivity to mupirocin compared to gentamycin in Dermato-venereology Clinic Dr. Hasan Sadikin General Hospital Bandung. An analytical cross sectional survey was intended from 19th September–31st October 2011 in 22 moderate to severe AD patients based on objective Scoring Atopic Dermatitis and 22 healthy persons. All subjects went through skin-scraping, MSRA was detected and sensitivity test was done for mupirocin and gentamycin. The subjects consisted of 10 boys and 12 girls in both group. Based on severity, 10/22 of AD subjects were in moderate AD and 12/22 were severe AD, 14/22 of AD subjects presented with acute lesions and 8/22 subjects had chronic lesions. In the group of AD patients, 17/22 growth of S. aureus were found with sensitivity against mupirocin 14/17 and gentamycin 11/17. In healthy individual group, no  S. aureus growth was found. Methicillin-resistant Staphylococcus aureus strain in AD patients was observed on 3/17 patients, and this was declared significantly different compared to healthy individuals (p <0.01). The isolates in 2/3 patients were sensitive to mupirocin and all patients were resistant to gentamycin. In conclusion, the patients of moderate to severe AD are prone to MRSA colonization and therefore requires mupirocin topical antibiotic treatment.  [MKB. 2013;45(1):35–43]Key words: Atopic dermatitis, gentamycin, MRSA, mupirocin DOI: http://dx.doi.org/10.15395/mkb.v45n1.203

Isolation and Identification of Pathogenic Fungi from Air Conditioners in Tutorial Rooms of the Faculty of Medicine, Universitas Padjadjaran

Althea Medical Journa Vol 1, No 1 (2014)
Publisher : Althea Medical Journa

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Abstract

Background: Awareness about health problems caused by air conditioner is very important. Thus, it is crucial to have knowledge about proper maintenance of air conditioner. At the Faculty of Medicine, Universitas Padjadjaran, air conditioners are used in every tutorial rooms. This study was performed to provide adequate information on the fungi, such as Aspergillus, Penicillium and Mucor, found in air conditioners.Methods: A descriptive laboratory study was used to identify the presence and the type of pathogenic fungi from air conditioners in tutorial rooms. Thirty-four samples were collected from the air outlet grille of the air conditioners and cultured on Sabouraud agar at 27°C for 2 weeks. Fungi presence were then identified microscopicallyResults: The results showed that the majority of air conditioners in tutorial rooms of the Faculty of Medicine, University Padjadjaran contained many types of fungus that grew in Sabouraud agar. From 34 samples, thirty two samples were positive and 2 samples were negative. Various fungus have been identified, those were Penicillium (37.5%), Aspergillus (25%), Mucor (2.5%) and unidentified (35%)Conclusions : The majority of air conditioners in tutorial rooms of the Faculty of Medicine, University Padjadjaran contained many types of opportunistic fungus. [AMJ.2014;1(1):21–4]Keywords : Air conditioner, Aspergillus, fungi, Mucor, Penicillium Isolasi dan Identifikasi Jamur Patogen dari Pendingin Udara di Kamar Tutorial dari Fakultas Kedokteran, Universitas PadjadjaranLatar Belakang: Kesadaran tentang masalah kesehatan yang disebabkan oleh pendingin udara sangat penting. Dengan demikian , sangat penting untuk memiliki pengetahuan tentang perawatan yang tepat dari pendingin udara. Di Fakultas Kedokteran, Universitas Padjadjaran, pendingin udara digunakan di setiap kamar tutorial . Penelitian ini dilakukan untuk memberikan informasi yang memadai tentang jamur, seperti Aspergillus, Penicillium dan Mucor, yang ditemukan di pendingin udara. Metode: Sebuah studi laboratorium deskriptif digunakan untuk mengevaluasi keberadaan dan jenis jamur patogen dari pendingin udara di kamar tutorial. Tiga puluh empat sampel dikumpulkan dari kisi-kisi pendingin udara dan dikultur pada Sabouraud agar pada 27 °C selama 2 minggu. Pertumbuhan jamur kemudian diidentifikasi secara mikroskopis.Hasil: Hasil penelitian menunjukkan bahwa pada sebagian besar pendingin udara di ruang tutorial Fakultas Kedokteran Universitas Padjajdjaran terdapat banyak jamur yang dapat tumbuh di Sabaroaud agar, 32 hasil positif dan 2 hasil negatif untuk pertumbuhan jamur . Jamur yang tumbuh adalah Penicillium (37,5%, Aspergillus (25%), Mucor (2,5%) dan tidak teridentifikasi (35%).Simpulan : Sebagian besar pendingin udara di ruang tutorial Fakultas Kedokteran Universitas Padjadjaran Jatinangor menunjukkan adanya pertumbuhan jamur oportunistikKata kunci : Aspergillus, jamur , Mucor , Penicillium, pendingin udara

Pengaruh Ekstrak Etanol Propolis Terhadap Aktivitas dan Kapasitas Fagositosis pada Kultur Makrofag yang Diinfeksi Enteropathogenic Escherichia coli (EPEC)

Majalah Kedokteran Bandung Vol 47, No 2 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Penanganan penyakit infeksi diperlukan suatu imunostimulator. Propolis adalah campuran resin yang dikumpulkan lebah dari tumbuh-tumbuhan, digunakan sebagai material isolasi sarang lebah, merupakan bahan yang berpotensi sebagai imunostimulator. Penelitian dilakukan di Laboratorium Penelitian dan Pengujian Terpadu Universitas Gadjah Mada, pada Januari–Maret 2013. Penelitian ini bertujuan menganalisis peningkatan aktivitas dan kapasitas fagositosis kultur makrofag yang diberi ekstrak etanol propolis terhadap Enteropathogenic Escherichia coli (EPEC). Desain penelitian eskperimen. Ke dalam kultur makrofag yang diperoleh dari tiga orang subjek, masing-masing diberi ekstrak etanol propolis 6,25, 12,5, 25, 50, dan 100 µg/mL serta kontrol tanpa ekstrak etanol propolis. Inkubasi dilakukan satu hari. Kultur diinfeksikan EPEC selama 30 menit, diwarnai dengan Giemsa dan diamati dengan mikroskop. Persentase aktivitas fagositosis diperoleh dengan menghitung jumlah makrofag yang aktif dalam 100 makrofag. Nilai kapasitas fagositosis diperoleh dengan menghitung jumlah bakteri yang difagosit oleh 50 makrofag. Uji analysis of variance (ANOVA) menunjukkan perbedaan signifikan aktivitas maupun kapasitas fagositosis kultur makrofag yang diberi ekstrak etanol propolis dibanding dengan kontrol (p=0,000). Uji Tukey HSD pada aktivitas fagositosis menunjukkan hasil signifikan antara kontrol dan konsentrasi 6,25 µg/mL (p=0,008), sedangkan pada kapasitas fagositosis terlihat antara kontrol dan konsentrasi 12,5 µg/mL (p=0,001). Simpulan, ekstrak etanol propolis meningkatkan aktivitas dan kapasitas fagositosis kultur makrofag terhadap EPEC dengan konsentrasi minimum 12,5 µg/mL. [MKB. 2015;47(2):102–8]Kata kunci: Aktivitas fagositosis, Enteropathogenic Escherichia coli (EPEC), kapasitas fagositosis, makrofag, propolis.    The Effect of Ethanolic Extract Propolis on Phagocytosis Activity and Capacity Macrophages Culture which Infected by Enteropathogenic Escherichia coli (EPEC)AbstractThe use of immunostimulant for treatment infectious diseases is needed. Propolis is mixture of resin collected from plants by bees, used as an insulating material in beehives, which has potential as immunostimulant. The research was performed at Laboratory of research and integrated testing Gadjah Mada University in January−March 2013. This experimental research is amied to analyze the increase of phagocytosis activity and capacity on macrophages culture which added by propolis ethanolic extract againts Enteropathogenic Escherichia coli (EPEC). The design research is experimental. Macrophage culture obtained from three subjects, individually culture supplemented with the propolis ethanolic extract 6.25, 12.5, 25, 50, and 100 µg/mL as well as control without the addition of propolis ethanolic extract. The cultures were incubated for one day. Macrophage cultures infected by EPEC for 30 minutes, stained by Giemsa and observed by microscope. Percentage of phagocytosis activity is determined by the number of active macrophages per 100 macrophages. Phagocytosis capacity value determined by counting the number of bacteria that phagocytized by 50 macrophages.  Analysis of variance (ANOVA) showed significantly different phagocytosis activity and capacity between macrophage cultures that added by propolis ethanolic extract and control (p=0.000). Tukey HSD analysis showed significantly different phagocytosis activity between control and subject with concentration 6.25 µg/mL (p=0.008) whereas on phagocytosis capacity appears between control and subject with concentration 12,5 µg/mL (p=0.001). In conclusion, propolis ethanolic extract is able to increase the phagocytosis activity and capacity of macrophages againts EPEC with minimum concentration 12.5µg/mL. [MKB. 2015;47(2):102–8] Key words: Enteropathogenic Escherichia coli (EPEC), macrophage, phagocytosis activity, phagocytosiscapacity, propolis DOI: 10.15395/mkb.v47n2.460

Enteric Pathogen Bacteria in Non-Broiler Chicken Egg Shells from Traditional Market and Supermarket, Jatinangor Subdistrict, West Java

Althea Medical Journal Vol 2, No 3 (2015)
Publisher : Althea Medical Journal

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Abstract

Background: Around 1.5 million of children dying annually due to diarrhea. Contaminated food is one of the sources of the diarrhea incidence (food borne diseases). Eggs are one of the least expensive forms of protein which is affordable by the community and is easily to find in either traditional or modern market/supermarkets.The objective of this study was to identify enteropathogenic bacteria contamination on non-broiler (ayam kampung) egg shell and to compare the findings between eggs sold in traditional and modern markets.Methods: This was a descriptive study performed at the Microbiology Laboratory of the Faculty of Medicine, Universitas Padjadjaran. A total of 40 eggs were used, 20 from two traditional markets and 20 from two modern markets. The eggs were swabbed using saline, dipped in tryptic soy broth and streaked on Mac Conkey agar. The collected data were analyzed and presented in tables.Results: Out of 40 samples, there were 19 positive cultures found from the traditional market and 16 from the modern market. There were 30 pink colonies indicating that they were lactose fermented, 5 transparent colonies indicated non-lactose fermentation, 4 showed no colony growth, and 1 grew an unidentified colony. The most found bacteria were Klebsiella sp. and Enterobacter sp. in both market. Conclusions: Eggs shells from traditional and modern markets are contaminated with Enteropathogenic microbes. DOI: 10.15850/amj.v2n3.487

Antimicrobial Suspectibility of Bacterial Isolated from Burn Unit in Dr. Hasan Sadikin General Hospital Bandung, Indonesia from June to September 2013

Althea Medical Journal Vol 2, No 3 (2015)
Publisher : Althea Medical Journal

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Abstract

Background: Development of infection in burned patients is a crucial matter because it may increase morbidity and mortality. Factors contributing to high risk for infections are disrupted skin barrier, dysfunction of the immune system and prolonged hospitalization. Crowded hospital condition and transfer of patients from one unit to another can affect development of infection caused by environmental factors. The aim of the study was to identify the antimicrobial suspectibility of bacterial isolated from Burn Unit in Dr. Hasan Sadikin General Hospital, Bandung.Methods: A descriptive study was carried out in Burn Unit Dr. Hasan Sadikin General Hospital Bandung, Indonesia, from June to September 2013.  A total of 30 environment samples were collected and cultured to identify the bacterias. Futhermore, the colonies  found, underwent antimicrobial susceptibility test.Results: Most common bacteria found from the environmental samples in Burn Unit was Staphylococcus saprophyticus (24%). Other bacterias found were Klebsiella pneumonia (17%), Acinetobacter baumanii (15%), Staphylococcus epidermidis (12%), Bacillus sp.(12%), Pseudomonas sp.(7%), Staphylococcus aureus (5%), Enterobacter aerogenes (5%) and Serratia sp. (2%). Some bacteria still had good sensitivity to antibiotics while the Enterobacteriaceae were almost completely resistant to antimicrobial used in the study.Conclusions: The most common bacteria found from the environment samples in Burn Unit is Staphylococcuss aprophyticus. Additionally, Klebsiella pneumoniae as one of the Enterobacteriaceae groups, appears as an emerging hospital associated infection pathogen with their resistant to many antimicrobials. DOI: 10.15850/amj.v2n3.592